LOCUS       sequence-238661-       10201 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-MET17 ICE plasmid used to facilitate yeast recombination 
            cloning and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10201)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 10201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-MET17
FEATURES             Location/Qualifiers
     source          1..10201
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      75..530
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(162..181)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     372..393
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        587..826
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             827..5005
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5406..5425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5525..5547
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5585..5603)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             5860..7194
                     /codon_start=1
                     /gene="S. cerevisiae MET17"
                     /gene_synonym="MET15; MET25"
                     /product="O-acetylserine and O-acetylhomoserine 
                     sulfhydrylase, required for sulfur amino acid synthesis"
                     /label=MET17
                     /translation="MPSHFDTVQLHAGQENPGDNAHRSRAVPIYATTSYVFENSKHGSQ
                     LFGLEVPGYVYSRFQNPTSNVLEERIAALEGGAAALAVSSGQAAQTLAIQGLAHTGDNI
                     VSTSYLYGGTYNQFKISFKRFGIEARFVEGDNPEEFEKVFDERTKAVYLETIGNPKYNV
                     PDFEKIVAIAHKHGIPVVVDNTFGAGGYFCQPIKYGADIVTHSATKWIGGHGTTIGGII
                     VDSGKFPWKDYPEKFPQFSQPAEGYHGTIYNEAYGNLAYIVHVRTELLRDLGPLMNPFA
                     SFLLLQGVETLSLRAERHGENALKLAKWLEQSPYVSWVSYPGLASHSHHENAKKYLSNG
                     FGGVLSFGVKDLPNADKETDPFKLSGAQVVDNLKLASNLANVGDAKTLVIAPYFTTHKQ
                     LNDKEKLASGVTKDLIRVSVGIEFIDDIIADFQQSFETVFAGQKP"
     promoter        7245..7349
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7350..7418,7419..8210)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7568..7587)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8381..8969
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8870..8889
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9123..9140
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9257..9278
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9293..9298,9299..9316,9317..9323)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9331..9347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9336..9358
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9355..9371
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9355..9371
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9390..9410
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9392..9410
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    9465..9968
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9999..10015
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9999..10015
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10049..10065)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10050..10066)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(10090..10109)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10091..10109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10119..10136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10119..10135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10128..10150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc
       61 tgaatggcga atggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
      121 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
      181 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
      241 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
      301 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
      361 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
      421 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
      481 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt cctgatgcgg
      541 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tagatcaatt ccacttgcaa
      601 ttacataaaa aattccggcg gtttttcgcg tgtgactcaa tgtcgaaata cctgcctaat
      661 gaacatgaac atcgcccaaa tgtatttgaa gacccgctgg gagaagttca agatatataa
      721 gtaacaagca gccaatagta taaaaaaaaa tctgagttta ttacctttcc tggaatttca
      781 gtgaaaaact gctaattata gagagatatc acagagttac tcactaatga ctaacgaaaa
      841 ggtctggata gagaagttgg ataatccaac tctttcagtg ttaccacatg actttttacg
      901 cccacaacaa gaaccttata cgaaacaagc tacatattcg ttacagctac ctcagctcga
      961 tgtgcctcat gatagttttt ctaacaaata cgctgtcgct ttgagtgtat gggctgcatt
     1021 gatatataga gtaaccggtg acgatgatat tgttctttat attgcgaata acaaaatctt
     1081 aagattcaat attcaaccaa cgtggtcatt taatgagctg tattctacaa ttaacaatga
     1141 gttgaacaag ctcaattcta ttgaggccaa tttttccttt gacgagctag ctgaaaaaat
     1201 tcaaagttgc caagatctgg aaaggacccc tcagttgttc cgtttggcct ttttggaaaa
     1261 ccaagatttc aaattagacg agttcaagca tcatttagtg gactttgctt tgaatttgga
     1321 taccagtaat aatgcgcatg ttttgaactt aatttataac agcttactgt attcgaatga
     1381 aagagtaacc attgttgcgg accaatttac tcaatatttg actgctgcgc taagcgatcc
     1441 atccaattgc ataactaaaa tctctctgat caccgcatca tccaaggata gtttacctga
     1501 tccaactaag aacttgggct ggtgcgattt cgtggggtgt attcacgaca ttttccagga
     1561 caatgctgaa gccttcccag agagaacctg tgttgtggag actccaacac taaattccga
     1621 caagtcccgt tctttcactt atcgcgacat caaccgcact tctaacatag ttgcccatta
     1681 tttgattaaa acaggtatca aaagaggtga tgtagtgatg atctattctt ctaggggtgt
     1741 ggatttgatg gtatgtgtga tgggtgtctt gaaagccggc gcaacctttt cagttatcga
     1801 ccctgcatat cccccagcca gacaaaccat ttacttaggt gttgctaaac cacgtgggtt
     1861 gattgttatt agagctgctg gacaattgga tcaactagta gaagattaca tcaatgatga
     1921 attggagatt gtttcaagaa tcaattccat cgctattcaa gaaaatggta ccattgaagg
     1981 tggcaaattg gacaatggcg aggatgtttt ggctccatat gatcactaca aagacaccag
     2041 aacaggtgtt gtagttggac cagattccaa cccaacccta tctttcacat ctggttccga
     2101 aggtattcct aagggtgttc ttggtagaca tttttccttg gcttattatt tcaattggat
     2161 gtccaaaagg ttcaacttaa cagaaaatga taaattcaca atgctgagcg gtattgcaca
     2221 tgatccaatt caaagagata tgtttacacc attattttta ggtgcccaat tgtatgtccc
     2281 tactcaagat gatattggta caccgggccg tttagcggaa tggatgagta agtatggttg
     2341 cacagttacc catttaacac ctgccatggg tcaattactt actgcccaag ctactacacc
     2401 attccctaag ttacatcatg cgttctttgt gggtgacatt ttaacaaaac gtgattgtct
     2461 gaggttacaa accttggcag aaaattgccg tattgttaat atgtacggta ccactgaaac
     2521 acagcgtgca gtttcttatt tcgaagttaa atcaaaaaat gacgatccaa actttttgaa
     2581 aaaattgaaa gatgtcatgc ctgctggtaa aggtatgttg aacgttcagc tactagttgt
     2641 taacaggaac gatcgtactc aaatatgtgg tattggcgaa ataggtgaga tttatgttcg
     2701 tgcaggtggt ttggccgaag gttatagagg attaccagaa ttgaataaag aaaaatttgt
     2761 gaacaactgg tttgttgaaa aagatcactg gaattatttg gataaggata atggtgaacc
     2821 ttggagacaa ttctggttag gtccaagaga tagattgtac agaacgggtg atttaggtcg
     2881 ttatctacca aacggtgact gtgaatgttg cggtagggct gatgatcaag ttaaaattcg
     2941 tgggttcaga atcgaattag gagaaataga tacgcacatt tcccaacatc cattggtaag
     3001 agaaaacatt actttagttc gcaaaaatgc cgacaatgag ccaacattga tcacatttat
     3061 ggtcccaaga tttgacaagc cagatgactt gtctaagttc caaagtgatg ttccaaagga
     3121 ggttgaaact gaccctatag ttaagggctt aatcggttac catcttttat ccaaggacat
     3181 caggactttc ttaaagaaaa gattggctag ctatgctatg ccttccttga ttgtggttat
     3241 ggataaacta ccattgaatc caaatggtaa agttgataag cctaaacttc aattcccaac
     3301 tcccaagcaa ttaaatttgg tagctgaaaa tacagtttct gaaactgacg actctcagtt
     3361 taccaatgtt gagcgcgagg ttagagactt atggttaagt atattaccta ccaagccagc
     3421 atctgtatca ccagatgatt cgtttttcga tttaggtggt cattctatct tggctaccaa
     3481 aatgattttt accttaaaga aaaagctgca agttgattta ccattgggca caattttcaa
     3541 gtatccaacg ataaaggcct ttgccgcgga aattgacaga attaaatcat cgggtggatc
     3601 atctcaaggt gaggtcgtcg aaaatgtcac tgcaaattat gcggaagacg ccaagaaatt
     3661 ggttgagacg ctaccaagtt cgtacccctc tcgagaatat tttgttgaac ctaatagtgc
     3721 cgaaggaaaa acaacaatta atgtgtttgt taccggtgtc acaggatttc tgggctccta
     3781 catccttgca gatttgttag gacgttctcc aaagaactac agtttcaaag tgtttgccca
     3841 cgtcagggcc aaggatgaag aagctgcatt tgcaagatta caaaaggcag gtatcaccta
     3901 tggtacttgg aacgaaaaat ttgcctcaaa tattaaagtt gtattaggcg atttatctaa
     3961 aagccaattt ggtctttcag atgagaagtg gatggatttg gcaaacacag ttgatataat
     4021 tatccataat ggtgcgttag ttcactgggt ttatccatat gccaaattga gggatccaaa
     4081 tgttatttca actatcaatg ttatgagctt agccgccgtc ggcaagccaa agttctttga
     4141 ctttgtttcc tccacttcta ctcttgacac tgaatactac tttaatttgt cagataaact
     4201 tgttagcgaa gggaagccag gcattttaga atcagacgat ttaatgaact ctgcaagcgg
     4261 gctcactggt ggatatggtc agtccaaatg ggctgctgag tacatcatta gacgtgcagg
     4321 tgaaaggggc ctacgtgggt gtattgtcag accaggttac gtaacaggtg cctctgccaa
     4381 tggttcttca aacacagatg atttcttatt gagatttttg aaaggttcag tccaattagg
     4441 taagattcca gatatcgaaa attccgtgaa tatggttcca gtagatcatg ttgctcgtgt
     4501 tgttgttgct acgtctttga atcctcccaa agaaaatgaa ttggccgttg ctcaagtaac
     4561 gggtcaccca agaatattat tcaaagacta cttgtatact ttacacgatt atggttacga
     4621 tgtcgaaatc gaaagctatt ctaaatggaa gaaatcattg gaggcgtctg ttattgacag
     4681 gaatgaagaa aatgcgttgt atcctttgct acacatggtc ttagacaact tacctgaaag
     4741 taccaaagct ccggaactag acgataggaa cgccgtggca tctttaaaga aagacaccgc
     4801 atggacaggt gttgattggt ctaatggaat aggtgttact ccagaagagg ttggtatata
     4861 tattgcattt ttaaacaagg ttggattttt acctccacca actcataatg acaaacttcc
     4921 actgccaagt atagaactaa ctcaagcgca aataagtcta gttgcttcag gtgctggtgc
     4981 tcgtggaagc tccgcagcag cttaaggttg agcattacgt atgatatgtc catgtacaat
     5041 aattaaatat gaattaggag aaagacttag cttcttttcg ggtgatgtca cttaaaaact
     5101 ccgagaataa tatataataa gagaataaaa tattagttat tgaataagaa ctgtaaatca
     5161 gctggcgtta gtctgctaat ggcagcttca tcttggttta ttgtagcatg aatcatattt
     5221 gccttttttt cctgtaattc aatgattctt gcttctatac tatcctcaat gcaaaacctt
     5281 gtgatcttca caggtcgata ctgaccaatt ctatgaactc tatcaccact ttgccattca
     5341 acactagggt tccaccatgg gtctaaaatg aatacttgcg aagctgatct atggtgcact
     5401 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     5461 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     5521 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     5581 aagggcctcg tgatacgcct atttttatag gttaatgtca tgatagccat cctcatgaaa
     5641 actgtgtaac ataataaccg aagtgtcgaa aaggtggcac cttgtccaat tgaacacgct
     5701 cgatgaaaaa aataagatat atataaggtt aagtaaagcg tctgttagaa aggaagtttt
     5761 tcctttttct tgctctcttg tcttttcatc tactatttcc ttcgtgtaat acagggtcgt
     5821 cagatacata gatacaattc tattaccccc atccatacaa tgccatctca tttcgatact
     5881 gttcaactac acgccggcca agagaaccct ggtgacaatg ctcacagatc cagagctgta
     5941 ccaatttacg ccaccacttc ttatgttttc gaaaactcta agcatggttc gcaattgttt
     6001 ggtctagaag ttccaggtta cgtctattcc cgtttccaaa acccaaccag taatgttttg
     6061 gaagaaagaa ttgctgcttt agaaggtggt gctgctgctt tggctgtttc ctccggtcaa
     6121 gccgctcaaa cccttgccat ccaaggtttg gcacacactg gtgacaacat cgtttccact
     6181 tcttacttat acggtggtac ttataaccag ttcaaaatct cgttcaaaag atttggtatc
     6241 gaggctagat ttgttgaagg tgacaatcca gaagaattcg aaaaggtctt tgatgaaaga
     6301 accaaggctg tttatttgga aaccattggt aatccaaagt acaatgttcc ggattttgaa
     6361 aaaattgttg caattgctca caaacacggt attccagttg tcgttgacaa cacatttggt
     6421 gccggtggtt acttctgtca gccaattaaa tacggtgctg atattgtaac acattctgct
     6481 accaaatgga ttggtggtca tggtactact atcggtggta ttattgttga ctctggtaag
     6541 ttcccatgga aggactaccc agaaaagttc cctcaattct ctcaacctgc cgaaggatat
     6601 cacggtacta tctacaatga agcctacggt aacttggcat acatcgttca tgttagaact
     6661 gaactattaa gagatttggg tccattgatg aacccatttg cctctttctt gctactacaa
     6721 ggtgttgaaa cattatcttt gagagctgaa agacacggtg aaaatgcatt gaagttagcc
     6781 aaatggttag aacaatcccc atacgtatct tgggtttcat accctggttt agcatctcat
     6841 tctcatcatg aaaatgctaa gaagtatcta tctaacggtt tcggtggtgt cttatctttc
     6901 ggtgtaaaag acttaccaaa tgccgacaag gaaactgacc cattcaaact ttctggtgct
     6961 caagttgttg acaatttaaa gcttgcctct aacttggcca atgttggtga tgccaagacc
     7021 ttagtcattg ctccatactt cactacccac aaacaattaa atgacaaaga aaagttggca
     7081 tctggtgtta ccaaggactt aattcgtgtc tctgttggta tcgaatttat tgatgacatt
     7141 attgcagact tccagcaatc ttttgaaact gttttcgctg gccaaaaacc atgagtgtgc
     7201 gtaatgagtt gtagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     7261 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     7321 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     7381 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     7441 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     7501 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     7561 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     7621 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     7681 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     7741 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     7801 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     7861 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7921 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7981 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     8041 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     8101 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     8161 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     8221 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     8281 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     8341 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     8401 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     8461 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     8521 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     8581 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     8641 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     8701 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     8761 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     8821 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     8881 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8941 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     9001 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     9061 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     9121 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     9181 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     9241 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     9301 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     9361 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     9421 tggagctcca ccgcggtggc ggccgcgcca cctgggtcct tttcatcacg tgctataaaa
     9481 ataattataa tttaaatttt ttaatataaa tatataaatt aaaaatagaa agtaaaaaaa
     9541 gaaattaaag aaaaaatagt ttttgttttc cgaagatgta aaagactcta gggggatcgc
     9601 caacaaatac taccttttat cttgctcttc ctgctctcag gtattaatgc cgaattgttt
     9661 catcttgtct gtgtagaaga ccacacacga aaatcctgtg attttacatt ttacttatcg
     9721 ttaatcgaat gtatatctat ttaatctgct tttcttgtct aataaatata tatgtaaagt
     9781 acgctttttg ttgaaatttt ttaaaccttt gtttattttt ttttcttcat tccgtaactc
     9841 ttctaccttc tttatttact ttctaaaatc caaatacaaa acataaaaat aaataaacac
     9901 agagtaaatt cccaaattat tccatcatta aaagatacga ggcgcgtgta agttacaggc
     9961 aagcgatccg tcctaagaag cgctaagagg gcggccgctc tagaactagt ggatcccccg
    10021 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
    10081 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
    10141 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
    10201 g
//
