LOCUS       sequence-238700-        7076 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Prey vector AT1G08590 X046_pECIA14 should be used with bait vector 
            AT1G08590 X046_pECIA2..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 7076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: AT1G08590 X046_pECIA14
FEATURES             Location/Qualifiers
     source          1..7076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     105..124
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     318..340
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     333..349
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        355..781
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     799..852
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2704..2727
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             4402..4425
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4441..4458
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4481..4498)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4531..4665
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4555..4574)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4609..4628
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4809..4825)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4809..4825)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4822..4844)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4833..4849
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4857..4863,4864..4881,4882..4887))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4902..4923
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5040..5057)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5211..5799)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5291..5310)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5970..6761,6762..6830))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6593..6612
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6831..6935)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7003..7021
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(join(7059..7076,1..5))
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
       61 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
      121 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata
      181 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
      241 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
      301 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattaattcg
      361 ttgcaggaca ggatgtggtg cccgatgtga ctagctcttt gctgcaggcc gtcctatcct
      421 ctggttccga taagagaccc agaactccgg ccccccaccg cccaccgcca cccccataca
      481 tatgtggtac gcaagtaaga gtgcctgcgc atgccccatg tgccccacca agagctttgc
      541 atcccataca agtccccaaa gtggagaacc gaaccaattc ttcgcgggca gaacaaaagc
      601 ttctgcacac gtctccactc gaatttggag ccggccggcg tgtgcaaaag aggtgaatcg
      661 aacgaaagac ccgtgtgtaa agccgcgttt ccaaaatgta taaaaccgag agcatctggc
      721 caatgtgcat cagttgtggt cagcagcaaa atcaagtgaa tcatctcagt gcaactaaag
      781 gggggatccg atctcaatat gaagttatgc atattactgg ccgtcgtggc ctttgttggc
      841 ctctcgctcg ggtctgaaac atttcaaaac tccgaacaag agatcttgtt ggccttcaag
      901 tcagaccttt ttgatccttc aaataatctc caagattgga agagacctga gaatgccaca
      961 acattttctg agttggttca ttgtcattgg acaggagttc actgtgatgc aaatggctat
     1021 gtggctaagc ttcttctctc caacatgaat ctcagcggaa acgtgtcaga tcagattcag
     1081 agcttccctt ctctgcaagc ccttgatctc agcaataacg catttgagtc ttctcttcct
     1141 aaatctcttt ctaatctcac gtctttgaag gtcattgatg tgagtgtgaa cagcttcttt
     1201 ggaaccttcc cttatggtct aggaatggca actggtctta cacacgtcaa tgcctcaagc
     1261 aacaattttt cggggtttct acctgaagat cttggcaacg caactacact tgaggttttg
     1321 gactttcgag gaggttactt cgagggctca gtgccttctt ctttcaagaa tctcaagaat
     1381 ctgaagtttc tcggtctttc gggtaacaac tttggtggaa aggtaccgaa ggtgattggt
     1441 gagctctctt ccttggagac cattattctt ggttacaatg gcttcatggg tgagattcca
     1501 gaagagtttg ggaaattgac gcgccttcag taccttgatt tggctgttgg aaaccttaca
     1561 ggccagattc catcttcttt ggggcagctg aaacaactta caacagttta tctctaccag
     1621 aaccgtctca cagggaaact tcctcgagaa cttggtggta tgacttctct tgtgtttctt
     1681 gatctgtctg ataaccagat cacaggagaa atacctatgg aagtaggtga gttgaagaat
     1741 ctgcagcttc taaatttgat gaggaatcaa ctcacgggaa tcattccgtc aaaaattgct
     1801 gaattaccaa accttgaggt tcttgagctg tggcagaact ctctcatggg ttcattaccg
     1861 gtgcatctcg gcaaaaattc cccattgaag tggttagacg tctcatcaaa caagctttca
     1921 ggagatatac catcaggatt gtgctattcc agaaacctca caaagctcat tctcttcaac
     1981 aactcattct caggtcagat tccggaggaa atcttctcat gtccgacttt agttcgtgtt
     2041 cgaatccaga aaaatcacat ttccggatct ataccagctg gttcaggtga cttaccaatg
     2101 cttcagcatc tagagctcgc aaaaaacaac ctcaccggta aaattccaga tgatatagcc
     2161 ttatccacat cactctcatt tatagacata tcatttaatc atctctcatc cctatcttcc
     2221 tccattttct caagtcctaa tcttcaaacc ttcattgcct cacataacaa ctttgccgga
     2281 aaaattccta accagattca agatcgtcca tctttgtctg tcctcgatct ttccttcaac
     2341 catttctcag gtggaatccc agaaaggatc gcttcttttg aaaaacttgt gagtctaaat
     2401 ctcaagagca accaattggt tggagaaatc cctaaggcat tagccgggat gcacatgtta
     2461 gctgttcttg atctctcaaa caactctttg acggggaata taccagcgga cttgggagct
     2521 tctccaacac tggagatgtt aaacgtgtca ttcaacaaac tcgacggacc cattccttca
     2581 aacatgctgt ttgcagccat agacccaaaa gacctcgtgg gaaacaatgg cttatgcggt
     2641 ggagttctac caccttgctc taagagccta gctttgtcag caaaaggccg aaatccaggg
     2701 agactggagg tgctgttcca gggaccaagc ggcaatcccg gcggcgatct ggccccccag
     2761 atgctgcgcg agctgcagga gaccaatgcc gccctgcagg acgtgcgcga gctgctgcgc
     2821 caccgcgtga aggagatcac cttcctgaag aacaccgtga tggagtgcga cgcctgcggc
     2881 atgcagccct ctagaggctc cggctccatc atcccagttg aggaggagaa cccggacttc
     2941 tggaaccgcg aggcagccga ggccctgggt gccgccaaga agctgcagcc tgcacagaca
     3001 gccgccaaga acctcatcat cttcctgggc gatgggatgg gggtgtctac ggtgacagct
     3061 gccaggatcc taaaagggca gaagaaggac aaactggggc ctgagatacc cctggccatg
     3121 gaccgcttcc catatgtggc tctgtccaag acatacaatg tagacaaaca tgtgccagac
     3181 agtggagcca cagccacggc ctacctgtgc ggggtcaagg gcaacttcca gaccattggc
     3241 ttgagtgcag ccgcccgctt taaccagtgc aacacgacac gcggcaacga ggtcatctcc
     3301 gtgatgaatc gggccaagaa agcagggaag tcagtgggag tggtaaccac cacacgagtg
     3361 cagcacgcct cgccagccgg cacctacgcc cacacggtga accgcaactg gtactcggac
     3421 gccgacgtgc ctgcctcggc ccgccaggag gggtgccagg acatcgctac gcagctcatc
     3481 tccaacatgg acattgacgt gatcctaggt ggaggccgaa agtacatgtt tcgcatggga
     3541 accccagacc ctgagtaccc agatgactac agccaaggtg ggaccaggct ggacgggaag
     3601 aatctggtgc aggaatggct ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact
     3661 gagctcatgc aggcttccct ggacccgtct gtgacccatc tcatgggtct ctttgagcct
     3721 ggagacatga aatacgagat ccaccgagac tccacactgg acccctccct gatggagatg
     3781 acagaggctg ccctgcgcct gctgagcagg aacccccgcg gcttcttcct cttcgtggag
     3841 ggtggtcgca tcgaccatgg tcatcatgaa agcagggctt accgggcact gactgagacg
     3901 atcatgttcg acgacgccat tgagagggcg ggccagctca ccagcgagga ggacacgctg
     3961 agcctcgtca ctgccgacca ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg
     4021 agctccatct tcgggctggc ccctggcaag gcccgggaca ggaaggccta cacggtcctc
     4081 ctatacggaa acggtccagg ctatgtgctc aaggacggcg cccggccgga tgttaccgag
     4141 agcgagagcg ggagccccga gtatcggcag cagtcagcag tgcccctgga cgaagagacc
     4201 cacgcaggcg aggacgtggc ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc
     4261 gtgcaggagc agaccttcat agcgcacgtc atggccttcg ccgcctgcct ggagccctac
     4321 accgcctgcg acctggcgcc ccccgccggc accaccgacg ccgcgcaccc gggttctaga
     4381 ggaagcacct ccggcgataa cgattacaag gatgatgatg ataaggccag cggcaccggt
     4441 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctaag
     4501 gcctgagctc gctgatcagc ctcgatcgag gatccagaca tgataagata cattgatgag
     4561 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4621 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4681 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     4741 ctctacaaat gtggtatggc tgattatgat cagtcgacct gcaggcatgc aagcttggcg
     4801 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     4861 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4921 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     4981 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5041 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5101 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5401 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5641 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     5821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     5881 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     5941 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6001 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6061 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6121 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6181 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6241 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6301 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6361 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6421 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6481 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6541 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6601 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6661 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6721 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6781 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     6841 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     6901 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     6961 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     7021 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagct
//
