LOCUS       sequence-238707-        6023 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bait vector AT1G56120 X070_pECIA2 should be used with prey vector 
            AT1G56120 X070_pECIA14..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Smakowska-Luzan E, Mott GA, Parys K, Stegmann M, Howton TC, 
            Layeghifard M, Neuhold J, Lehner A, Kong J, Grunwald K, Weinberger 
            N, Satbhai SB, Mayer D, Busch W, Madalinski M, Stolt-Bergner P, 
            Provart NJ, Mukhtar MS, Zipfel C, Desveaux D, Guttman DS, Belkhadir 
            Y
  TITLE     An extracellular network of Arabidopsis leucine-rich repeat receptor
            kinases.
  JOURNAL   Nature. 2018 Jan 18;553(7688):342-346. doi: 10.1038/nature25184. 
            Epub 2018 Jan 10.
  PUBMED    29320478
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25184"; journalName: "Nature"; date: "2018-01-18- 
            18"; volume: "553"; issue: "7688"; pages: "342-346"
COMMENT     Sequence Label: AT1G56120 X070_pECIA2
FEATURES             Location/Qualifiers
     source          1..6023
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     40..58
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(96..118)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     218..237
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     431..453
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     446..462
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        468..894
                     /label=MT promoter
                     /note="Drosophila metallothionein promoter"
     sig_peptide     912..965
                     /codon_start=1
                     /product="Drosophila BiP signal sequence"
                     /label=BiP signal sequence
                     /translation="MKLCILLAVVAFVGLSLG"
     CDS             2721..2744
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             3450..3491
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             3501..3518
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3541..3558)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3591..3725
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3615..3634)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3669..3688
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3869..3885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3869..3885)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3882..3904)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3893..3909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3917..3923,3924..3941,3942..3947))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3962..3983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4100..4117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4271..4859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4351..4370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5030..5821,5822..5890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5653..5672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5891..5995)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
       61 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
      121 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
      181 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
      241 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
      301 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
      361 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
      421 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattaat tcgttgcagg
      481 acaggatgtg gtgcccgatg tgactagctc tttgctgcag gccgtcctat cctctggttc
      541 cgataagaga cccagaactc cggcccccca ccgcccaccg ccacccccat acatatgtgg
      601 tacgcaagta agagtgcctg cgcatgcccc atgtgcccca ccaagagctt tgcatcccat
      661 acaagtcccc aaagtggaga accgaaccaa ttcttcgcgg gcagaacaaa agcttctgca
      721 cacgtctcca ctcgaatttg gagccggccg gcgtgtgcaa aagaggtgaa tcgaacgaaa
      781 gacccgtgtg taaagccgcg tttccaaaat gtataaaacc gagagcatct ggccaatgtg
      841 catcagttgt ggtcagcagc aaaatcaagt gaatcatctc agtgcaacta aaggggggat
      901 ccgatctcaa tatgaagtta tgcatattac tggccgtcgt ggcctttgtt ggcctctcgc
      961 tcgggtccat cttcgcggct tggaagattc gggcgccgag ggaatggaac atcagcggcg
     1021 aactttgctc cggcgtcgcc atcgacgcca gtgtactcga ctcaaaccat gcctacaatc
     1081 ctctcatcaa atgcgactgt agtttccaaa actccacaat ctgccgcata aataacataa
     1141 aggtttatgc gatagatgtt gtaggaccta tacctccaga gctctggacc ttaacatacc
     1201 tcaccaatct gaacctaggt caaaattatc tcacaggctc actgtctcct gctattggga
     1261 atttgactcg gatgcagtgg atgacttttg gaatcaacgc gttgtctggc cctattccta
     1321 aggaaatcgg tttgcttacg gatttaagat tacttggtat tagttcaaat aacttttccg
     1381 gttccttacc agctgagatt gggagttgta caaaactaca gcaaatgtac atcgatagtt
     1441 ctggactcag cggaggaata cctttatcat ttgctaattt tgtggagttg gaagtcgctt
     1501 ggattatgga tgtggaactt acaggtcgga taccagactt tataggattc tggaccaaac
     1561 ttactacctt gaggatcctc ggaactggtt tgagcggccc aataccgtcg tcattttcca
     1621 acttaattgc tttgacagaa ctgaggcttg gtgatatatc caatggaagc tcttctcttg
     1681 atttcatcaa agacatgaaa tctctaagtg tattagtatt gaggaacaac aatctcactg
     1741 ggacaatacc gtctactatt ggaggataca caagtttgca acaagttgat ttgagcttca
     1801 acaaattaca tggaccaatt ccggcttcac ttttcaactt aagtcgactt actcacttgt
     1861 ttctgggaaa caacacgttg aatggatcct tgcccactct aaaggggcag tctctgagca
     1921 atttagatgt gtcgtacaat gatttgtctg gaagtcttcc ttcatgggtc agcttaccag
     1981 atttgaagct caatctagtt gctaacaact tcacactgga aggtcttgac aacagggttt
     2041 tatcaggact acattgcctg cagaagaact tcccctgcaa tcgaggcgaa ggaatctatt
     2101 ataacttttc gatcaactgc ggaggcccag agataaggtc tgttagtgga gcactatttg
     2161 aaaaggagga cgcggatctt ggaccagctt cgtttgttgt gagtgctgca aagagatggg
     2221 cagccagtag tgtaggaaat tttgctggca gtagcaacaa tatatacata gctacttcac
     2281 tagcacaatt tatcaacact atggactcag agctttttca gtcagcacga ctttctgcat
     2341 cttccctaag gtattatggg ttggggctag aaaatggagg gtacaccgta acacttcagt
     2401 ttgctgaagt acaaattgaa ggttctaact cttggaaagg tattggaaga cgacgtttta
     2461 acatttatgt ccagggaaga cttgttgaaa aggattttga tatacgcaga acagctggtg
     2521 gctcttcggt tcgagcagtt cagagagaat ataaaacaaa tgtatcagaa aatcatctcg
     2581 aagtccatct tttctgggct ggaaaaggaa catgttgtat tcctatccaa ggggcttatg
     2641 ggccattaat agcggccgtc agtgcgacac cagatttcac accaactgtg gctaataggc
     2701 caccatcaaa gggaaagagc ctggaggtgc tgttccaggg accagggagc gacaaaactc
     2761 acacatgccc accgtgccca gcacctgaac tcctgggggg accgtcagtc ttcctcttcc
     2821 ccccaaaacc caaggacacc ctcatgatct cccggacccc tgaggtcaca tgcgtggtgg
     2881 tggacgtgag ccacgaagac cctgaggtca agttcaactg gtacgtggac ggcgtggagg
     2941 tgcataatgc caagacaaag ccgcgggagg agcagtacaa cagcacgtac cgtgtggtca
     3001 gcgtcctcac cgtcctgcac caggactggc tgaatggcaa ggagtacaag tgcaaggtct
     3061 ccaacaaagc cctcccagcc cccatcgaga aaaccatctc caaagccaaa gggcagcccc
     3121 gagaaccaca ggtgtacacc ctgcccccat cccgggagga gatgaccaag aaccaggtca
     3181 gcctgacctg cctggtcaaa ggcttctatc ccagcgacat cgccgtggag tgggagagca
     3241 atgggcagcc ggagaacaac tacaagacca cgcctcccgt gctggactcc gacggctcct
     3301 tcttcctcta cagcaagctc accgtggaca agagcaggtg gcagcagggg aacgtcttct
     3361 catgctccgt gatgcatgag ggtctgcaca accactacac gcagaagagc ctctccctgt
     3421 ctccgggtaa atctagaggg cccttcgaag gtaagcctat ccctaaccct ctcctcggtc
     3481 tcgattctac gcgtaccggt catcatcacc atcaccattg agtttaaacc cgctgatcag
     3541 cctcgactgt gccttctaag gcctgagctc gctgatcagc ctcgatcgag gatccagaca
     3601 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3661 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3721 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     3781 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagtcgacct
     3841 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3901 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3961 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4021 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4081 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4681 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5341 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5641 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6001 ttccccgaaa agtgccacct gac
//
