LOCUS       sequence-238732-        4335 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  obtaining full Cas12a RNP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4335)
  AUTHORS   Qiao J, Li W, Lin S, Sun W, Ma L, Liu Y
  TITLE     Co-expression of Cas9 and single-guided RNAs in Escherichia coli 
            streamlines production of Cas9 ribonucleoproteins.
  JOURNAL   Commun Biol. 2019 May 3;2:161. doi: 10.1038/s42003-019-0402-x. 
            eCollection 2019.
  PUBMED    31069270
REFERENCE   2  (bases 1 to 4335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-05-3"; volume: "2:161. doi"; pages: " 
            10.1038/s42003-019-0402-x. eCollection 2019"
COMMENT     Sequence Label: pCold CL7-Cas12a
FEATURES             Location/Qualifiers
     source          1..4335
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             409..432
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atgagcaaaa gcaatgaacc gggtaaagca accggtgaag gtaaaccggt taataacaaa
       61 tggctgaaca atgccggtaa agatctgggt agtccggttc cggatcgtat tgcaaataaa
      121 ctgcgtgata aagaattcga gagcttcgat gattttcgtg aaaccttttg ggaagaagtt
      181 agcaaagatc ctgaactgag caaacagttt agccgcaata acaatgatcg tatgaaagtt
      241 ggtaaagcac cgaaaacacg tacccaggat gttagcggta aacgtacctc atttgaactg
      301 aatcatcaga aaccgattga acagaatggt ggcgtttatg atatggataa cattagcgtt
      361 gttaccccga aacgcaacat tgatattgaa ggcggtggtg gtggttcttt ggaggttttg
      421 ttccagggtc caatgtcaat ttatcaagaa tttgtgaaca aatatagcct gagcaaaacc
      481 ctgcgttttg aactgattcc gcagggtaaa accctggaaa acattaaagc acgtggtctg
      541 attctggatg atgaaaaacg tgccaaagac tacaaaaaag ccaaacaaat catcgataaa
      601 taccaccagt tcttcatcga agaaattctg agcagcgttt gcattagcga agatctgctg
      661 cagaattatt ccgacgttta tttcaaactg aaaaaaagcg acgatgataa cctgcagaaa
      721 gatttcaaaa gcgccaaaga taccatcaaa aaacaaatta gcgagtatat caaagacagc
      781 gagaaattca aaaacctgtt caaccagaat ctgatcgatg ccaaaaaagg tcaagaaagc
      841 gatctgatcc tgtggctgaa acagagcaaa gataatggca tcgaactgtt taaagccaac
      901 agcgatatta ccgatattga tgaagcactg gaaatcatca aaagctttaa aggttggacc
      961 acctacttta aaggctttca cgaaaatcgc aaaaacgtgt atagcagcaa tgatattccg
     1021 accagcatta tctatcgcat cgttgatgat aatctgccta aatttctgga aaataaagcc
     1081 aaatatgaaa gcctgaaaga caaagcaccg gaagcaatta actatgagca gatcaaaaaa
     1141 gatctggccg aagaactgac ctttgacatt gattacaaaa ccagcgaagt taaccagcgt
     1201 gtttttagcc tggatgaagt ttttgaaatt gccaacttca acaactacct gaatcagagc
     1261 ggtatcacca aattcaatac cattatcggt ggcaaattcg tgaatggcga aaataccaaa
     1321 cgcaaaggca tcaacgaata cattaatctg tatagccagc agattaacga taaaacgctg
     1381 aaaaaataca aaatgagcgt gctgttcaaa caaattctgt cagataccga aagcaaaagc
     1441 ttcgtgattg acaaactgga agatgatagt gatgttgtta ccaccatgca gagcttttat
     1501 gaacaaatcg cagcgtttaa aaccgtggaa gagaaatcca ttaaagaaac cctgagcctg
     1561 ctgtttgatg atctgaaagc acagaaactg gacctgtcca aaatctactt caaaaacgat
     1621 aaatccctga ccgatctgag ccagcaggtt ttcgatgatt atagcgttat tggcaccgca
     1681 gttctggaat atatcacaca gcagattgca ccgaaaaatc tggataatcc gagcaaaaaa
     1741 gaacaagagc tgatcgccaa aaaaaccgag aaagcgaaat atctgagcct ggaaacaatt
     1801 aaactggccc tggaagaatt taacaaacac cgcgacattg ataaacagtg ccgctttgaa
     1861 gaaatcctgg caaattttgc agcaatcccg atgatctttg atgaaattgc gcagaataaa
     1921 gataacctgg cacagatcag catcaaatat cagaatcagg gaaaaaaaga cctgctgcaa
     1981 gcaagtgccg aagatgatgt taaagcgatt aaagatctgc tggatcagac caataacctg
     2041 ctgcataaac tgaaaatctt tcacattagc cagagcgagg ataaagcgaa cattctggat
     2101 aaagatgagc acttctatct ggtgtttgaa gagtgttatt ttgagctggc aaatattgtg
     2161 ccgctgtata acaaaatccg caactatatt acccagaaac cgtatagcga cgaaaaattc
     2221 aaactgaact ttgagaatag caccctggcc aatggttggg ataaaaacaa agaaccggat
     2281 aataccgcca tcctgttcat taaagatgat aaatactatc tgggcgtgat gaacaaaaaa
     2341 aacaacaaaa tcttcgacga taaagccatc aaagagaata aaggcgaagg ttacaaaaaa
     2401 atcgtgtaca aactgctgcc tggtgcgaat aaaatgctgc cgaaagtgtt ttttagcgcc
     2461 aaatccatca aattctataa cccgagcgaa gatattctgc gtattcgtaa tcatagcacc
     2521 cataccaaaa atggtagtcc gcagaaaggc tatgaaaaat tcgagttcaa cattgaggat
     2581 tgccgcaaat tcatcgactt ctacaaacag tccattagca aacatccgga atggaaagac
     2641 tttggttttc gttttagcga tacccagcgc tataacagca ttgatgaatt ttatcgcgaa
     2701 gtggaaaacc agggctataa actgacattt gaaaacatca gcgagagcta tattgatagc
     2761 gttgtgaatc agggtaaact gtacctgttt cagatctata acaaagactt tagcgcctat
     2821 agcaaaggtc gtccgaatct gcataccctg tattggaaag cactgttcga tgaacgtaat
     2881 ctgcaggatg ttgtctacaa actgaatggt gaagcagaac tgttttatcg caaacagagt
     2941 atcccgaaaa aaatcaccca tccggcaaaa gaagcaatcg cgaacaaaaa caaagataac
     3001 ccgaaaaaag aaagcgtgtt cgagtatgat ctgatcaaag ataaacgctt caccgaagat
     3061 aaattctttt tccattgccc gatcaccatc aactttaaaa gcagcggtgc gaacaaattc
     3121 aacgatgaaa tcaatctgct gctgaaagaa aaagccaacg atgttcatat tctgagcatt
     3181 gatcgtggtg aacgtcatct ggcctattac accctggttg atggtaaagg caatattatc
     3241 aaacaggaca ccttcaacat tatcggcaat gatcgtatga aaaccaacta ccatgataaa
     3301 ctggcagcca ttgaaaaaga tcgtgatagc gcacgtaaag attggaaaaa aatcaacaac
     3361 attaaagaaa tgaaagaagg ctacctgagc caggttgttc atgaaatcgc caaactggtg
     3421 attgaatata atgccattgt ggtgttcgag gatctgaact tcggtttcaa acgtggtcgt
     3481 ttcaaagttg agaaacaggt gtatcaaaaa ctggaaaaaa tgctgatcga aaaactgaat
     3541 tacctggtgt tcaaagacaa cgaattcgat aaaaccggtg gtgttctgcg tgcatatcag
     3601 ctgaccgcac cttttgaaac cttcaaaaaa atgggtaaac agaccggcat catctattat
     3661 gttccggcag gttttacctc caaaatttgt ccggttaccg gctttgttaa tcagctgtat
     3721 ccgaaatatg agagcgttag caaaagccaa gagtttttca gcaaatttga taaaatctgc
     3781 tataacctgg acaaaggcta ctttgaattc agctttgact ataaaaactt tggcgataaa
     3841 gcagccaaag gcaaatggac cattgcaagc tttggtagcc gtctgattaa ctttcgtaac
     3901 agcgacaaaa accataactg ggatacccgt gaagtttatc cgaccaaaga gctggaaaaa
     3961 ctgctgaaag attacagcat tgaatatggt catggcgaat gtattaaagc cgcaatttgt
     4021 ggtgagtccg acaaaaaatt ctttgcaaaa ctgaccagcg tgctgaatac cattctgcag
     4081 atgcgtaata gcaaaaccgg caccgaactg gattatctga ttagtccggt tgcagatgtg
     4141 aacggcaatt ttttcgatag ccgtcaggct ccgaaaaata tgccgcagga tgcagatgca
     4201 aatggtgcct atcatattgg cctgaaaggt ctgatgctgc tgggtcgcat taaaaacaat
     4261 caagaaggca aaaaactgaa cctggtgatc aaaaacgaag agtattttga gttcgtgcag
     4321 aataggaata actag
//
