LOCUS       sequence-23875-e         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2w5-Prd
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             215..484
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             494..517
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cttacgcgca aatgcnacct cccaattcaa gttgcggaca acaacccttt gcaacagcaa
       61 tcatcacaaa ntaccgtacc aaacgtcctc aaccaaatta accaaatctt ttctccagag
      121 gagcaacgca gcttattaca agaagccatc gaaacctgca agaattttga aaaaacacaa
      181 ttgtaagtct cggctccaag aaggagatat acccatggca cgcgtaactg ttcaggacgc
      241 tgtagagaaa attggtaacc gttttgacct ggtactggtc gccgcgcgtc gcgctcgtca
      301 gatgcaggta ggcggaaagg acccgctcgt accggaagaa aacgataaaa ccactgtaat
      361 cgcgctgcgc gaaatcgaag aaggtctgat caacaaccag atcctcgacg ttcgcgaacg
      421 ccaggaacag caagagcagg aagccgctga attacaagcc gttaccgcta ttgctgaagg
      481 tcgtgcggcc gcggactaca aggatgacga cgacaagttc cggaccggtt ccaagacacc
      541 cccccatggt accaacagcg gccaggggcg cgtcaatcaa ctaggtggag ttttcatcaa
      601 cggtcgtcct ttgcccaaca atattcgtct taaantcgtc gagatgnccg ccgatggcat
      661 tncggccctg tgtgaactcg anacagcttc atgngtcnaa tggnttgcgt atcgaagatc
      721 ctgaatcgct accgggagac tggcttcctt agaccaggcg taattggggg ntccaagccg
      781 agnatagccn cncccgaaat cgaaa
//
