LOCUS       sequence-23876-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2wL-Prd
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             50..319
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             329..352
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cnaatgantg attcggattc ctggaactct aaaccaaaga gaggacacca tggcacgcgt
       61 aactgttcag gacgctgtag agaaaattgg taaccgtttt gacctggtac tggtcgccgc
      121 gcgtcgcgct cgtcagatgc aggtaggcgg aaaggacccg ctcgtaccgg aagaaaacga
      181 taaaaccact gtaatcgcgc tgcgcgaaat cgaagaaggt ctgatcaaca accagatcct
      241 cgacgttcgc gaacgccagg aacagcaaga gcaggaagcc gctgaattac aagccgttac
      301 cgctattgct gaaggtcgtg cggccgcgga ctacaaggat gacgacgaca agttccggac
      361 cggttccaag acaccccccc atggtaccaa cagcggccag gggcgcgtca atcaactagg
      421 tggagttttc atcaacggtc gtcctttgcc caacaatatt cgtcttaaaa tcgtcgagat
      481 ggccgccgat ggcattcggc cctgtgtgat ctccagacag ctacgtgtgt cccatggctg
      541 cgtatcgaag atcctgaatc gctaccagga gactggctcc attagaccag gcgtaattgg
      601 tggctccaag ccgaggatag ccacgcccga aatcgagaac catngaaaan cnnnnnannn
      661 nnntnccngg ggcatgttct cgtggggaga tcagggagaa gctgatccgc gagggtgtct
      721 gcgacaggag cacagcacca tctgtgtccg ccatatcgcg cctggtgcgc ggccgagatg
      781 ctccattgga cantgatatg tcttctgcct ctggatctcc gg
//
