LOCUS       sequence-238761-       15528 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targets a gene and expresses zebrafish mitfa specifically in 
            zebrafish melanocytes .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15528)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 15528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR U6:gRNA, mitfa:Cas9
FEATURES             Location/Qualifiers
     source          1..15528
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2222..2238
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2222..2238
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2272..2283,2284..2471)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7262..7396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_RNA        7795..7870
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     7883..7899
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7907..7927
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    7990..8124
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8126..8150
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8179..8197
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(8251..8271)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8287..12387)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8366..8374
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(12394..12414)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      12423..12432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(12467..12486)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12468..12486)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12496..12512)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(12514..12538)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14412..14432)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(14452..14468)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(14841..15003,15004..15015)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     15364..15385
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atgccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tcattctatt ttcccttgct attaccaaac caattgaatt gcgctgatgc
     2521 ccagtttaat ttaaatgtta tttattctgc ctatgaaaat cgttttcaca ttatatgaaa
     2581 ttggtcagac atgttcattg gtcctttgga agtgacgtca tgtcacatct attaccacaa
     2641 tgcacagcac cttgacctgg aaattaggga aattataaca gtcaatcagt ggaagaaaat
     2701 ggaggaagta tgtgattcat cagcagctgc gagcagcaca gtccaaaatc agccacagga
     2761 tcaagagcac ccgtggccgt atcttcgcag atctcgaata agagattgct acggagagat
     2821 attctgctga ttaggagctt gtctatgcta atttggtttt gttcaattca cctatgtcgc
     2881 aagtttttgg actgtaggag aaaaccggag aacccagggg atatccacat gagcacgagg
     2941 acaacatgta aactccacac agaaccatgg actggcctgt tagagacttg gaccagtgac
     3001 gttcttgctt tgaggcaaca gtgctaacaa ctgggctgct gtgccgctct ttcgaggaaa
     3061 tgaaaggaac tctggcggcc tatgatgagt taggaattgt ctgattggtg aatcatacat
     3121 aggctaatgt gggaccagct gtagtcagtc ataagcacgt gattctctcg aaattagttt
     3181 ataaataaac ttcactaaac tccaaccgtt cattctgttt tagatgttgc ctttaaactt
     3241 gccttaagat gttaataaag ttgctttttg gttgttgaca aagctaggca taggacgata
     3301 accgttttca aggtataccc cggtttgaaa aagtcaaggg tttaaaacca ccaatatttt
     3361 ctgctatacc gttcctaagg tatatttaag attttttatt tattttttat ttttttgttt
     3421 tttaggacaa cagcattttc agcagaaaag agatacaaaa atgcttttta aattgtaaag
     3481 aaatctgttt ttgaaactaa tgaagacagc agaagtcgat cattcattga aattattaag
     3541 cttgacatgt tttctgttcc aaaatatttt ttcaatgttt ctcaaaataa aatatattat
     3601 gtttaaagtt tttgtttttt actaagacat taaaaaaaat aatatatttt agagtggcaa
     3661 tcacaatacc atcaaactgt gatattttta tcaaaggtta tcataccatc agaatcttat
     3721 accccatgcc tagacacagc tgttctctct atctctctat gttaatgcag tttccactcc
     3781 attgacgaga tggttctctg taccacatac tcatccccat tttacaatag ctacatttgc
     3841 tcaagatgta gtcaactcaa acaatgcaga gacaacaaac tgttgactta tcttttctat
     3901 cgtaattcag cccttttggg aaatgagcat caacacacaa tttagagtgg aagttgggtt
     3961 aaggcagact cattttttac acattacaac cagtgattat gtcaattatt atttaaaaaa
     4021 aattcagtac agtatatgca catgctactt tgtttatgat tgttagcttt tttaactaca
     4081 gttcatttct atgctttctg tgcaaatgca caacagttac tgatttactc tgatgataat
     4141 tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga cttacaatca agccccaaag
     4201 tgttcatacg tctggcaaat tctgacttaa agttactttt attcaaccag ctagtttttt
     4261 tttgactgta aatgacacgt aagataataa gatgatgtac atgaggcatc attgtagaaa
     4321 aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt gattttgttt gtgcgcactt
     4381 acatacgtgt gcacaatatt tcaactctta agaaaaaaat gcaattacca aaggcccatc
     4441 agacaacaaa gttctctttc ctatcttttg ctcttcaaca tttcagagat aactccatct
     4501 taaactgtct tgtttttaga ccaacagtgc tagtgattgt atgccggatg ctgagaacaa
     4561 acaatgtttt atgcaggatg agtggaacta aagatgaaac gttagttagc cgtaagccag
     4621 tttcagttta gacactagta acccatcgtc tcaaagaggc ttttgtcgaa tcggaccggt
     4681 gacgtcaaat gaactttata aaggcataaa ttcatcgttt gggtaaaaaa aacaatatga
     4741 gaagagcaat cccctcccac ccccttaaat acattttgtg tcttttcttt ggcattgaca
     4801 tcttcagctg gccaagacga ctggtcagtt cttgcacatc acctaaattc acaaagggaa
     4861 tacttttttg aagctaacac atagttgaac atgttggaga tgctcgagta cagtcactac
     4921 caggttcaga cccacctgga aaccccctcg aagtaccaca tccagcagag ccagaggcag
     4981 caggtgaagc actacctgtc cagtgcactg ggagccaagc tgagcccaca ggccagcaca
     5041 gggcccggcc ccagccagcc cgccgagcac ggcatgaccc cgggacccgg agccagcgct
     5101 cccaacagcc ctatggccct tctcaccctc aactgtgaga aagagatgga cgatgtaatt
     5161 gaagacatta taagtttgga atcaagctac agtgatgaca ttcttgggtt catggatgca
     5221 ggacttcaaa tgacaaacac gattccagtt tcagcaaacc tgctggacat gtacagcaat
     5281 catgctcttc ctccagctgg agtttccatc agtaactcct gcccctcaag cctgccggcc
     5341 gtcaaaaggg aattatccgt tactccatct ccgggcatga tgcacataat ggacaaagct
     5401 ggaccatgtg gcaagtttga ctcttatcaa agacctgatg gctttccagt agaagcagaa
     5461 gtcagagccc tggcaaaaga gagacaaaag aaggacaacc acaacctcat tgaacgaaga
     5521 aggcggttta atatcaatga cagaattaag gagctgggga ctttaattcc caagtcaaat
     5581 gatccagaca tgaggtggaa taaaggcacc atcctgaaag catcagtgga ttacattagg
     5641 aaattgcaga aagagcagca gaaagcaaaa gagctggaaa acagacagaa gagactagaa
     5701 cacgcaaaca gacatctcct gctccgtatt caggaacttg aaatgcaagc ccgtgctcat
     5761 ggactcaccg ttgtagcttc ttccagcctt tattccgctg agttagtggc tcgagcaatc
     5821 aaacaagagc ctgggatggg ggactgcaca tccaatttgt acccacacct tcccagtcct
     5881 gacatgtccc gtcctaccac tctggacctc aacaacggca ccatcagcta caatgacagt
     5941 cccacggagg acggcgagcc gggggtctac gacagcccaa acaaggcctc caccaagctg
     6001 gaggacatgc ttatggacaa caccctgtct ccggtgggca gcagcgaccc gctgttgtcc
     6061 tccggatccc cagtaccttc caacagtagc ggcagcagca gtatggacga acatgacaat
     6121 ggctgttagc aatgccaact aaatttcatg aaccaaagtt agacaattag atttgtgtgt
     6181 gtgtgtgtct tgtaagtacc agtgttacat ccagatcatg aagagcgcaa tttcgtaaag
     6241 tattaatctt gtatttcaga atatttagtg tgcttgtgta tatatttttt catttatcat
     6301 gtcagagtct agtcaagaat gaaatcgtgt aaacaattag ttgatttttg aaggattctc
     6361 gcggatgata taaagagtag cttgaataaa aactgttgag gtgtctttta tttgcaggct
     6421 tggcttattg ttgtcctcaa atgcagctcc atttcttggg gtgtacatct tgcaagggtg
     6481 gaggacatgt ctaggcatgt acaactggat tcactttcat tcagtcattc attttctttt
     6541 tggcttagcc cctttattaa tcaggggttg ccacagttga atgaactgcc aacttatgcc
     6601 aacaaacaaa tccagcggat gcccttgcag ctgcaaccca tcactgggaa acacccacca
     6661 cagtaccatt cactttagac aatttagctt acccaattca cctatagcgc atgtctttgg
     6721 gctgtaggtg aaactggagc acccggagga aacccacacg aacatgggga gaacatgcaa
     6781 actccacaca gaaatgaccc aaccaagtct cgaaccagcg accttcttgc tgtaaggcga
     6841 caatgctacc cactgcacca ccacgatgcc ctggaaaaac tttttacatt gttatttaac
     6901 attaagtcca aaagtatttc tgcagtattt ctgaaatttg ttttattttc tagtggaaga
     6961 gctgtgatgc aagttctcca taataattag aaaaaaaatg cccaaattgc tttgatttta
     7021 gggatagttc acccaaaaat gaattttttt ttattaatta atcatcctca tgtcgttcca
     7081 attccctgag acctttgttc atctttaaaa cacaaaaaat gatattttgg atacaatctg
     7141 agatctgatc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7201 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7261 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7381 ttatcatgtc tggatcatca tcgatgcgtc ttttgttctg gtcatcaagg aggggggaat
     7441 gttctgcgca tgcctgtggg ggagggagaa ggacacgtca ctgaaaacgt ccctgcatca
     7501 caccgagaca cccaatcact caagccgaga ccagataatt ttgcatatgc tttacagttt
     7561 gaaaaatacc acggtaaacc ctcacacaaa ctctggattt gagatctttc aggttttatc
     7621 agtttgcagg tttatgtcac catgatatag ggtcagactt gatctaaggg agctgaataa
     7681 gtggtttagt cactcaccac ctcccaaaaa catacccaga agtccctggt atatatagct
     7741 ctccctccag ctcttggttc ggctagcact cctcagtgac gaggagtcag tagcgtttta
     7801 gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc
     7861 gagtcggtgc ttttttggta ccgtaaaacg acggccagtg aattatcaac tatgtataat
     7921 aaagttggaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     7981 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8041 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8101 aatgtatctt atcatgtctg gatcccccac tttgtacaag aaagctgggt gctatgacca
     8161 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg
     8221 cggtggcggc cgctctagat tccgcggtca cacctttctc ttcttcttag gagatctcac
     8281 cggtgagtcg ccacccagct gagacaggtc aatccttgtt tcgtacaggc cggtaatact
     8341 ctgatggatc agtgtagcgt ccagcacctc cttagtggag gtgtacctct ttctatcgat
     8401 tgtagtgtcg aagtacttga aagctgcagg tgcgcccagg tttgtcagag tgaacagatg
     8461 aatgatattt tcggcctgct ccctgatggg cttatctctg tgtttgttgt aagcagacag
     8521 gaccttatcc agatttgcgt cggccaggat cactctttta gagaactcgc taatctgttc
     8581 aatgatctcg tccagataat gcttgtgctg ctccacaaac agctgtttct gttcgttatc
     8641 ctctggagat cctttcagct tctcgtagtg gcttgccaga tacaggaagt tcacgtactt
     8701 agaaggcagg gccagttcat tgcctttctg cagctctcca gcgcttgcca gcatgcgttt
     8761 cctgccgttt tccagctcaa acagactgta cttgggcagt ttaatgatca ggtctttctt
     8821 cacttcctta taacctttgg cctccagaaa atcgattggg ttcttctcga aagagcttct
     8881 ctccataatt gtgataccca gcagctcctt gacagatttc agtttcttgc ttttgccctt
     8941 ctccacctta gcgaccacca ggacggagta tgccacagtg ggagagtcaa agcctccata
     9001 cttcttaggg tcccagtctt tctttctagc gatcagctta tcagagttgc gttttggcag
     9061 aatactttct tttgagaaac cgcctgtctg cacctcagtt ttcttgacga tattcacttg
     9121 aggcatgctc aggaccttcc tcacggttgc aaaatctctg cctttgtccc acacgatttc
     9181 gcctgtctct ccgtttgtct caatcagggg ccttttgcgg atttctccat tggccagggt
     9241 gatctctgtc ttaaagaaat tcatgatgtt tgagtaaaag aaatacttgg cagtagcttt
     9301 tccaatttcc tgctcggact tggcgatcat ttttctgaca tcgtacacct tatagtctcc
     9361 gtacacgaat tcgctctcca gttttgggta tttcttgatc agtgcggtac cgaccacggc
     9421 gttcaggtat gcgtcatggg catggtgata attgttaatc tcgcggacct tataaaactg
     9481 gaaatctttc ctaaagtcgc tgaccagttt actcttcagg gtaatgacct tcacttctct
     9541 gatcagtttg tcattctcat cgtactttgt gttcatgcga ctatccagaa tctgtgcgac
     9601 gtgtttagtg atctggcgtg tttccaccag ctgcctcttg atgaatccag ctttgtccag
     9661 ttctgacagt ccaccgcgct ctgcttttgt caggttatca aacttcctct gagtgatcag
     9721 tttggcattc agcagctgtc tccagtagtt cttcattttc ttgaccactt cctcactagg
     9781 gacattatct gattttcctc tgtttttgtc ggacctggtc agcaccttgt tgtcaatgct
     9841 gtcatctttg atgaaactct gggggacgat atgatccacg tcgtaatcag acagcctgtt
     9901 aatatccagc tcctggtcca cgtacatatc tcttccattc tgcagatagt acagatacag
     9961 cttctcattc tgcagctgtg tgttttccac tgggtgctct ttcaggatct ggctacccag
    10021 ctccttaatg ccttcctcga ttcttttcat cctctctcta ctgttcttct gacccttctg
    10081 ggttgtctga ttttcgcgag ccatctcaat cacgatgttt tcgggcttat ggcccatgac
    10141 tttcaccagc tcatcgacga ttttcactgt ctgcagaatg cctttcttga tggctgggct
    10201 tccagccagg tttgcaatct gctcatgcag ggagtggccc tgtccagaca cctgagcctt
    10261 ctggatgtcc tctttgaagg tcagactgtc atcgtgaatc agctgcataa aattgcggtt
    10321 ggcgaagcca tcacttttca gaaagtccag gattgtcttt cctgactgtt tatccctaat
    10381 gccgttgatc agcttcctgc tcagtctacc ccagccagtg tagcgccttc tcttcagctg
    10441 tttcatcacc ttgtcatcga acagatgagc ataggttttc agtctttcct cgatcattcc
    10501 tctgtcctca aacagggtca gtgtcagcac aatgtcttcc aggatatcct cattttcctc
    10561 gttgtccagg aagtctttat ccttaatgat cttcagcaga tcgtggtaag cgcccaggct
    10621 tgcgttaaat ctgtcttcga ctccgctgat ctccacggaa tcgaagcatt caattttctt
    10681 gaagtagtcc tccttcagct gcttgacagt cactttcctg ttggttttaa acagcagatc
    10741 cacgattgct ttcttctgct ctcctgacag gaaggctggc tttctcatgc cctcggtgac
    10801 gtatttcacc tttgtcagtt cgttatagac agtgaaatac tcgtacagca gggagtgctt
    10861 aggcagcact ttctcattgg gcaggttttt gtcaaaatta gtcattctct cgatgaagga
    10921 ctgtgcagag gcgcccttat cgaccacttc ctcgaagttc caaggagtaa tggtttcctc
    10981 ggatttgcga gtcatccagg caaatctgct gtttccgcga gccagtggac ccacatagta
    11041 agggattctg aaggtcagaa ttttttcgat cttctcgcgg ttatctttca gaaaggggta
    11101 gaagtcttcc tggcgcctca ggatagcgtg cagctctccc agatgaatct ggtgtgggat
    11161 ggaaccgtta tcaaatgtgc gctgcttcct cagcaggtcc tctctattca gtttcaccag
    11221 cagttcctca gttccgtcca tcttctccag aataggtttg ataaacttgt agaattcctc
    11281 ctgtgatgcg cctccgtcga tataaccggc gtagccgttc ttggactgat caaagaaaat
    11341 ttccttgtac ttctctggca gctgctgcct gaccagagcc ttcagcaggg tcagatcctg
    11401 atggtgctcg tcatatctct tgatcatgct ggcactcaga ggtgcttttg taatctcgct
    11461 gttcactctc agaatatctg acagcaggat tgcgtcggac agatttttgg cagccaggaa
    11521 caggtcagcg tactgatctc cgatctgtgc cagcaggtta tccaggtcat cgtcatatgt
    11581 gtcctttgac agctgcagtt tggcgtcctc agccagatca aaattgcttt tgaagttagg
    11641 agtcaggccc agactcagag cgatcagatt tccaaacaga ccgttcctct tttctccggg
    11701 cagctgtgca atcaggttct ccagtctgcg tgacttggac agcctggctg acaggatagc
    11761 ttttgcgtcc actctggaag cattaatagg gttttcctca aacagctgat tgtaaatctg
    11821 gaccagctgg atgaacagct tgtccacatc ggaattgtcg gggttcagat cgccctcgat
    11881 cagaaaatga cccctgaact taatcatgtg ggccagagcc agatagatca gtctcaggtc
    11941 ggctttatca gtagagtcag ccagtttctt gcgcagatga tagatggttg ggtatttttc
    12001 gtgataggcg acctcgtcca caatgtttcc aaagatagga tggcgctcgt gtttcttatc
    12061 ttcctcgacc aggaaacttt cctccagcct atgaaagaat gaatcgtcca ccttagccat
    12121 ttcgttagag aaaatctcct gcaggtaaca gattctattc ttccttctgg tatagcgcct
    12181 tcttgctgtt cttttcaggc gtgttgcttc ggcagtctct ccagaaccga acagcagggc
    12241 tccaatcaga tttttcttga tgctgtgcct gtcggtgtta cccagcacct taaatttctt
    12301 gctagggacc ttgtagtcgt ctgtaatcac tgcccaaccc acggagttag ttccaatatc
    12361 caggccgatg ctatacttct tatccatgct tcccaccttt ctcttcttct taggtggaga
    12421 agccatggtg gcgtcgacct cgaggggggg cccggtaccc aattcgccct atagtgagtc
    12481 gtattacgcg cgctcactgg ccgtcgtttt acaagcctgc ttttttgtac aaacttgcgt
    12541 tcaactatgt gttagcttca aaaaagtatt ccctttgtga atttaggtga tgtgcaagaa
    12601 ctgaccagtc gtcttggcca gctgaagatg tcaatgccaa agaaaagaca caaaatgtat
    12661 ttaagggggt gggaggggat tgctcttctc atattgtttt ttttacccaa acgatgaatt
    12721 tatgccttta taaagttcat ttgacgtcac cggtccgatt cgacaaaagc ctctttgaga
    12781 cgatgggtta ctagtgtcta aactgaaact ggcttacggc taactaacgt ttcatcttta
    12841 gttccactca tcctgcataa aacattgttt gttctcagca tccggcatac aatcactagc
    12901 actgttggtc taaaaacaag acagtttaag atggagttat ctctgaaatg ttgaagagca
    12961 aaagatagga aagagaactt tgttgtctga tgggcctttg gtaattgcat ttttttctta
    13021 agagttgaaa tattgtgcac acgtatgtaa gtgcgcacaa acaaaatcac agacgtacag
    13081 ttcatattca gattcatatt catttttttt tctacaatga tgcctcatgt acatcatctt
    13141 attatcttac gtgtcattta cagtcaaaaa aaaactagct ggttgaataa aagtaacttt
    13201 aagtcagaat ttgccagacg tatgaacact ttggggcttg attgtaagtc tttttttttt
    13261 ttttgttgtt gttggatgta ctggcacaat tatcatcaga gtaaatcagt aactgttgtg
    13321 catttgcaca gaaagcatag aaatgaactg tagttaaaaa agctaacaat cataaacaaa
    13381 gtagcatgtg catatactgt actgaatttt ttttaaataa taattgacat aatcactggt
    13441 tgtaatgtgt aaaaaatgag tctgccttaa cccaacttcc actctaaatt gtgtgttgat
    13501 gctcatttcc caaaagggct gaattacgat agaaaagata agtcaacagt ttgttgtctc
    13561 tgcattgttt gagttgacta catcttgagc aaatgtagct attgtaaaat ggggatgagt
    13621 atgtggtaca gagaaccatc tcgtcaatgg agtggaaact gcattaacat agagagatag
    13681 agagaacagc tgtgtctagg catggggtat aagattctga tggtatgata acctttgata
    13741 aaaatatcac agtttgatgg tattgtgatt gccactctaa aatatattat tttttttaat
    13801 gtcttagtaa aaaacaaaaa ctttaaacat aatatatttt attttgagaa acattgaaaa
    13861 aatattttgg aacagaaaac atgtcaagct taataatttc aatgaatgat cgacttctgc
    13921 tgtcttcatt agtttcaaaa acagatttct ttacaattta aaaagcattt ttgtatctct
    13981 tttctgctga aaatgctgtt gtcctaaaaa acaaaaaaat aaaaaataaa taaaaaatct
    14041 taaatatacc ttaggaacgg tatagcagaa aatattggtg gttttaaacc cttgactttt
    14101 tcaaaccggg gtataccttg aaaacggtta tcgtcctatg cctagctttg tcaacaacca
    14161 aaaagcaact ttattaacat cttaaggcaa gtttaaaggc aacatctaaa acagaatgaa
    14221 cggttggagt ttagtgaagt ttatttataa actaatttcg agagaatcac gtgcttatga
    14281 ctgactacag ctggtcccac attagcctat gtatgattca ccaatcagac aattcctaac
    14341 tcatcatagg ccgccagagt tcctttcatt tcctcgaaag agcggcacag cagcccagtt
    14401 gttagcactc acaacttttc tatacaaagt tgatagcttg gcgtaatcat ggtcatagct
    14461 gtttcctgct cgagggccca tctggcctgt gtttcagaca ccagggagtc tctgctcacg
    14521 tttcctgcta tttgcagcct ctctatcaag actaatacac ctcttcccgc atcggctgcc
    14581 tgtgagaggc ttttcagcac tgcaggattg cttttcagcc ccaaaagagc taggcttgac
    14641 actaacaatt ttgagaatca gcttctactg aagttaaatc tgaggtttta caactttgag
    14701 tagcgtgtac tggcattaga ttgtctgtct tatagtttga taattaaata caaacagttc
    14761 taaagcagga taaaaccttg tatgcatttc atttaatgtt ttttgagatt aaaagcttaa
    14821 acaagaatct ctagttttct ttcttgcttt tacttttact tccttaatac tcaagtacaa
    14881 ttttaatgga gtactttttt acttttactc aagtaagatt ctagccagat acttttactt
    14941 ttaattgagt aaaattttcc ctaagtactt gtactttcac ttgagtaaaa tttttgagta
    15001 ctttttacac ctctgtcaag aactcctgga caaacctctg acctgtgtgg aacagagtgg
    15061 atatgggtgt ctgaacagat attcacgtct tttgcagatc agagggcatt tctggtgttg
    15121 tgcaatgacc tgggtccaac aggtaggcgt actcgtatcc aagagcattg ttggacagga
    15181 ggtagtctcc attcctataa agaggaagga atggcaccat gtagtagccg tcattgtggc
    15241 caattggtgc attggtacgt gggtagatgg accggggagg ctgatgagtt cttagccagc
    15301 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
    15361 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
    15421 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
    15481 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtgg
//
