LOCUS       sequence-238762-       10041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-KanMx ICE plasmid used to facilitate yeast recombination 
            cloning and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10041)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 10041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-KanMx
FEATURES             Location/Qualifiers
     source          1..10041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      127..582
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(214..233)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     424..445
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        639..878
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             879..5057
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5458..5477)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5577..5599
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5637..5655)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            complement(5708..7064)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(5708..5905)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5911..6720)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6634..6653
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(6721..7064)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        7137..7241
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7242..7310,7311..8102)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7460..7479)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8273..8861
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8762..8781
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9015..9032
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9149..9170
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9185..9190,9191..9208,9209..9215)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9223..9239
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9228..9250
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9247..9263
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9247..9263
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9282..9302
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9284..9302
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    9357..9860
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9891..9907
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9891..9907
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9941..9957)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9942..9958)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9982..10001)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9983..10001)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10011..10028)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10011..10027)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(join(10020..10041,1))
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
       61 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      121 gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
      181 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
      241 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
      301 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
      361 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
      421 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
      481 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
      541 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct
      601 ccttacgcat ctgtgcggta tttcacaccg catagatcaa ttccacttgc aattacataa
      661 aaaattccgg cggtttttcg cgtgtgactc aatgtcgaaa tacctgccta atgaacatga
      721 acatcgccca aatgtatttg aagacccgct gggagaagtt caagatatat aagtaacaag
      781 cagccaatag tataaaaaaa aatctgagtt tattaccttt cctggaattt cagtgaaaaa
      841 ctgctaatta tagagagata tcacagagtt actcactaat gactaacgaa aaggtctgga
      901 tagagaagtt ggataatcca actctttcag tgttaccaca tgacttttta cgcccacaac
      961 aagaacctta tacgaaacaa gctacatatt cgttacagct acctcagctc gatgtgcctc
     1021 atgatagttt ttctaacaaa tacgctgtcg ctttgagtgt atgggctgca ttgatatata
     1081 gagtaaccgg tgacgatgat attgttcttt atattgcgaa taacaaaatc ttaagattca
     1141 atattcaacc aacgtggtca tttaatgagc tgtattctac aattaacaat gagttgaaca
     1201 agctcaattc tattgaggcc aatttttcct ttgacgagct agctgaaaaa attcaaagtt
     1261 gccaagatct ggaaaggacc cctcagttgt tccgtttggc ctttttggaa aaccaagatt
     1321 tcaaattaga cgagttcaag catcatttag tggactttgc tttgaatttg gataccagta
     1381 ataatgcgca tgttttgaac ttaatttata acagcttact gtattcgaat gaaagagtaa
     1441 ccattgttgc ggaccaattt actcaatatt tgactgctgc gctaagcgat ccatccaatt
     1501 gcataactaa aatctctctg atcaccgcat catccaagga tagtttacct gatccaacta
     1561 agaacttggg ctggtgcgat ttcgtggggt gtattcacga cattttccag gacaatgctg
     1621 aagccttccc agagagaacc tgtgttgtgg agactccaac actaaattcc gacaagtccc
     1681 gttctttcac ttatcgcgac atcaaccgca cttctaacat agttgcccat tatttgatta
     1741 aaacaggtat caaaagaggt gatgtagtga tgatctattc ttctaggggt gtggatttga
     1801 tggtatgtgt gatgggtgtc ttgaaagccg gcgcaacctt ttcagttatc gaccctgcat
     1861 atcccccagc cagacaaacc atttacttag gtgttgctaa accacgtggg ttgattgtta
     1921 ttagagctgc tggacaattg gatcaactag tagaagatta catcaatgat gaattggaga
     1981 ttgtttcaag aatcaattcc atcgctattc aagaaaatgg taccattgaa ggtggcaaat
     2041 tggacaatgg cgaggatgtt ttggctccat atgatcacta caaagacacc agaacaggtg
     2101 ttgtagttgg accagattcc aacccaaccc tatctttcac atctggttcc gaaggtattc
     2161 ctaagggtgt tcttggtaga catttttcct tggcttatta tttcaattgg atgtccaaaa
     2221 ggttcaactt aacagaaaat gataaattca caatgctgag cggtattgca catgatccaa
     2281 ttcaaagaga tatgtttaca ccattatttt taggtgccca attgtatgtc cctactcaag
     2341 atgatattgg tacaccgggc cgtttagcgg aatggatgag taagtatggt tgcacagtta
     2401 cccatttaac acctgccatg ggtcaattac ttactgccca agctactaca ccattcccta
     2461 agttacatca tgcgttcttt gtgggtgaca ttttaacaaa acgtgattgt ctgaggttac
     2521 aaaccttggc agaaaattgc cgtattgtta atatgtacgg taccactgaa acacagcgtg
     2581 cagtttctta tttcgaagtt aaatcaaaaa atgacgatcc aaactttttg aaaaaattga
     2641 aagatgtcat gcctgctggt aaaggtatgt tgaacgttca gctactagtt gttaacagga
     2701 acgatcgtac tcaaatatgt ggtattggcg aaataggtga gatttatgtt cgtgcaggtg
     2761 gtttggccga aggttataga ggattaccag aattgaataa agaaaaattt gtgaacaact
     2821 ggtttgttga aaaagatcac tggaattatt tggataagga taatggtgaa ccttggagac
     2881 aattctggtt aggtccaaga gatagattgt acagaacggg tgatttaggt cgttatctac
     2941 caaacggtga ctgtgaatgt tgcggtaggg ctgatgatca agttaaaatt cgtgggttca
     3001 gaatcgaatt aggagaaata gatacgcaca tttcccaaca tccattggta agagaaaaca
     3061 ttactttagt tcgcaaaaat gccgacaatg agccaacatt gatcacattt atggtcccaa
     3121 gatttgacaa gccagatgac ttgtctaagt tccaaagtga tgttccaaag gaggttgaaa
     3181 ctgaccctat agttaagggc ttaatcggtt accatctttt atccaaggac atcaggactt
     3241 tcttaaagaa aagattggct agctatgcta tgccttcctt gattgtggtt atggataaac
     3301 taccattgaa tccaaatggt aaagttgata agcctaaact tcaattccca actcccaagc
     3361 aattaaattt ggtagctgaa aatacagttt ctgaaactga cgactctcag tttaccaatg
     3421 ttgagcgcga ggttagagac ttatggttaa gtatattacc taccaagcca gcatctgtat
     3481 caccagatga ttcgtttttc gatttaggtg gtcattctat cttggctacc aaaatgattt
     3541 ttaccttaaa gaaaaagctg caagttgatt taccattggg cacaattttc aagtatccaa
     3601 cgataaaggc ctttgccgcg gaaattgaca gaattaaatc atcgggtgga tcatctcaag
     3661 gtgaggtcgt cgaaaatgtc actgcaaatt atgcggaaga cgccaagaaa ttggttgaga
     3721 cgctaccaag ttcgtacccc tctcgagaat attttgttga acctaatagt gccgaaggaa
     3781 aaacaacaat taatgtgttt gttaccggtg tcacaggatt tctgggctcc tacatccttg
     3841 cagatttgtt aggacgttct ccaaagaact acagtttcaa agtgtttgcc cacgtcaggg
     3901 ccaaggatga agaagctgca tttgcaagat tacaaaaggc aggtatcacc tatggtactt
     3961 ggaacgaaaa atttgcctca aatattaaag ttgtattagg cgatttatct aaaagccaat
     4021 ttggtctttc agatgagaag tggatggatt tggcaaacac agttgatata attatccata
     4081 atggtgcgtt agttcactgg gtttatccat atgccaaatt gagggatcca aatgttattt
     4141 caactatcaa tgttatgagc ttagccgccg tcggcaagcc aaagttcttt gactttgttt
     4201 cctccacttc tactcttgac actgaatact actttaattt gtcagataaa cttgttagcg
     4261 aagggaagcc aggcatttta gaatcagacg atttaatgaa ctctgcaagc gggctcactg
     4321 gtggatatgg tcagtccaaa tgggctgctg agtacatcat tagacgtgca ggtgaaaggg
     4381 gcctacgtgg gtgtattgtc agaccaggtt acgtaacagg tgcctctgcc aatggttctt
     4441 caaacacaga tgatttctta ttgagatttt tgaaaggttc agtccaatta ggtaagattc
     4501 cagatatcga aaattccgtg aatatggttc cagtagatca tgttgctcgt gttgttgttg
     4561 ctacgtcttt gaatcctccc aaagaaaatg aattggccgt tgctcaagta acgggtcacc
     4621 caagaatatt attcaaagac tacttgtata ctttacacga ttatggttac gatgtcgaaa
     4681 tcgaaagcta ttctaaatgg aagaaatcat tggaggcgtc tgttattgac aggaatgaag
     4741 aaaatgcgtt gtatcctttg ctacacatgg tcttagacaa cttacctgaa agtaccaaag
     4801 ctccggaact agacgatagg aacgccgtgg catctttaaa gaaagacacc gcatggacag
     4861 gtgttgattg gtctaatgga ataggtgtta ctccagaaga ggttggtata tatattgcat
     4921 ttttaaacaa ggttggattt ttacctccac caactcataa tgacaaactt ccactgccaa
     4981 gtatagaact aactcaagcg caaataagtc tagttgcttc aggtgctggt gctcgtggaa
     5041 gctccgcagc agcttaaggt tgagcattac gtatgatatg tccatgtaca ataattaaat
     5101 atgaattagg agaaagactt agcttctttt cgggtgatgt cacttaaaaa ctccgagaat
     5161 aatatataat aagagaataa aatattagtt attgaataag aactgtaaat cagctggcgt
     5221 tagtctgcta atggcagctt catcttggtt tattgtagca tgaatcatat ttgccttttt
     5281 ttcctgtaat tcaatgattc ttgcttctat actatcctca atgcaaaacc ttgtgatctt
     5341 cacaggtcga tactgaccaa ttctatgaac tctatcacca ctttgccatt caacactagg
     5401 gttccaccat gggtctaaaa tgaatacttg cgaagctgat ctatggtgca ctctcagtac
     5461 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     5521 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     5581 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     5641 cgtgatacgc ctatttttat aggttaatgt catgatctgg atggcggcgt tagtatcgaa
     5701 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
     5761 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
     5821 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
     5881 aaacaagaat ctttttattg tcagtactga ttagaaaaac tcatcgagca tcaaatgaaa
     5941 ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa
     6001 tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc
     6061 gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt
     6121 atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg
     6181 catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc
     6241 atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct
     6301 gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc
     6361 atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttgcc
     6421 ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt
     6481 cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt
     6541 ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa
     6601 tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa
     6661 atcagcatcc atgttggaat ttaatcgcgg cctcgaaacg tgagtctttt ccttacccat
     6721 ggttgtttat gttcggatgt gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt
     6781 atatgaaaga agaacctcag tggcaaatcc taacctttta tatttctcta caggggcgcg
     6841 gcgtggggac aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc
     6901 gaggagccgt aatttttgct tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat
     6961 tatacatggg gatgtatggg ctaaatgtac gggcgacagt cacatcatgc ccctgagctg
     7021 cgcacgtcaa gactgtcaag gagggtattc tgggcctcca tgtcgctggc cgggtgaccc
     7081 ggcggggacg aggcaagcta aacaagacgt caggtggcac ttttcgggga aatgtgcgcg
     7141 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     7201 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     7261 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     7321 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     7381 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     7441 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     7501 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     7561 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     7621 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     7681 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     7741 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     7801 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     7861 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     7921 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     7981 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     8041 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     8101 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     8161 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     8221 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     8281 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     8341 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     8401 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     8461 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     8521 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     8581 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     8641 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8701 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8761 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8821 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8881 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8941 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     9001 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     9061 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     9121 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     9181 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     9241 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     9301 gggaacaaaa gctggagctc caccgcggtg gcggccgcgc cacctgggtc cttttcatca
     9361 cgtgctataa aaataattat aatttaaatt ttttaatata aatatataaa ttaaaaatag
     9421 aaagtaaaaa aagaaattaa agaaaaaata gtttttgttt tccgaagatg taaaagactc
     9481 tagggggatc gccaacaaat actacctttt atcttgctct tcctgctctc aggtattaat
     9541 gccgaattgt ttcatcttgt ctgtgtagaa gaccacacac gaaaatcctg tgattttaca
     9601 ttttacttat cgttaatcga atgtatatct atttaatctg cttttcttgt ctaataaata
     9661 tatatgtaaa gtacgctttt tgttgaaatt ttttaaacct ttgtttattt ttttttcttc
     9721 attccgtaac tcttctacct tctttattta ctttctaaaa tccaaataca aaacataaaa
     9781 ataaataaac acagagtaaa ttcccaaatt attccatcat taaaagatac gaggcgcgtg
     9841 taagttacag gcaagcgatc cgtcctaaga agcgctaaga gggcggccgc tctagaacta
     9901 gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc gacctcgagg
     9961 gggggcccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
    10021 gttttacaac gtcgtgactg g
//
