LOCUS       sequence-238764-        9804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-NatMx ICE plasmid used to facilitate yeast recombination 
            cloning and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-NatMx
FEATURES             Location/Qualifiers
     source          1..9804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      126..581
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(213..232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     423..444
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        638..877
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             878..5056
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5457..5476)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5576..5598
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5636..5654)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            complement(5707..6826)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      complement(5707..5904)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5910..6482)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(6483..6826)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        6899..7003
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7004..7072,7073..7864)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7222..7241)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8035..8623
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8524..8543
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8777..8794
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8911..8932
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8947..8952,8953..8970,8971..8977)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8985..9001
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8990..9012
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9009..9025
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9009..9025
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9044..9064
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9046..9064
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    9119..9622
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9653..9669
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9653..9669
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(9703..9719)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9704..9720)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(9744..9763)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9745..9763)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9773..9790)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9773..9789)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9782..9804)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
       61 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
      121 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
      181 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
      241 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
      301 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
      361 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
      421 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
      481 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
      541 atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg gtattttctc
      601 cttacgcatc tgtgcggtat ttcacaccgc atagatcaat tccacttgca attacataaa
      661 aaattccggc ggtttttcgc gtgtgactca atgtcgaaat acctgcctaa tgaacatgaa
      721 catcgcccaa atgtatttga agacccgctg ggagaagttc aagatatata agtaacaagc
      781 agccaatagt ataaaaaaaa atctgagttt attacctttc ctggaatttc agtgaaaaac
      841 tgctaattat agagagatat cacagagtta ctcactaatg actaacgaaa aggtctggat
      901 agagaagttg gataatccaa ctctttcagt gttaccacat gactttttac gcccacaaca
      961 agaaccttat acgaaacaag ctacatattc gttacagcta cctcagctcg atgtgcctca
     1021 tgatagtttt tctaacaaat acgctgtcgc tttgagtgta tgggctgcat tgatatatag
     1081 agtaaccggt gacgatgata ttgttcttta tattgcgaat aacaaaatct taagattcaa
     1141 tattcaacca acgtggtcat ttaatgagct gtattctaca attaacaatg agttgaacaa
     1201 gctcaattct attgaggcca atttttcctt tgacgagcta gctgaaaaaa ttcaaagttg
     1261 ccaagatctg gaaaggaccc ctcagttgtt ccgtttggcc tttttggaaa accaagattt
     1321 caaattagac gagttcaagc atcatttagt ggactttgct ttgaatttgg ataccagtaa
     1381 taatgcgcat gttttgaact taatttataa cagcttactg tattcgaatg aaagagtaac
     1441 cattgttgcg gaccaattta ctcaatattt gactgctgcg ctaagcgatc catccaattg
     1501 cataactaaa atctctctga tcaccgcatc atccaaggat agtttacctg atccaactaa
     1561 gaacttgggc tggtgcgatt tcgtggggtg tattcacgac attttccagg acaatgctga
     1621 agccttccca gagagaacct gtgttgtgga gactccaaca ctaaattccg acaagtcccg
     1681 ttctttcact tatcgcgaca tcaaccgcac ttctaacata gttgcccatt atttgattaa
     1741 aacaggtatc aaaagaggtg atgtagtgat gatctattct tctaggggtg tggatttgat
     1801 ggtatgtgtg atgggtgtct tgaaagccgg cgcaaccttt tcagttatcg accctgcata
     1861 tcccccagcc agacaaacca tttacttagg tgttgctaaa ccacgtgggt tgattgttat
     1921 tagagctgct ggacaattgg atcaactagt agaagattac atcaatgatg aattggagat
     1981 tgtttcaaga atcaattcca tcgctattca agaaaatggt accattgaag gtggcaaatt
     2041 ggacaatggc gaggatgttt tggctccata tgatcactac aaagacacca gaacaggtgt
     2101 tgtagttgga ccagattcca acccaaccct atctttcaca tctggttccg aaggtattcc
     2161 taagggtgtt cttggtagac atttttcctt ggcttattat ttcaattgga tgtccaaaag
     2221 gttcaactta acagaaaatg ataaattcac aatgctgagc ggtattgcac atgatccaat
     2281 tcaaagagat atgtttacac cattattttt aggtgcccaa ttgtatgtcc ctactcaaga
     2341 tgatattggt acaccgggcc gtttagcgga atggatgagt aagtatggtt gcacagttac
     2401 ccatttaaca cctgccatgg gtcaattact tactgcccaa gctactacac cattccctaa
     2461 gttacatcat gcgttctttg tgggtgacat tttaacaaaa cgtgattgtc tgaggttaca
     2521 aaccttggca gaaaattgcc gtattgttaa tatgtacggt accactgaaa cacagcgtgc
     2581 agtttcttat ttcgaagtta aatcaaaaaa tgacgatcca aactttttga aaaaattgaa
     2641 agatgtcatg cctgctggta aaggtatgtt gaacgttcag ctactagttg ttaacaggaa
     2701 cgatcgtact caaatatgtg gtattggcga aataggtgag atttatgttc gtgcaggtgg
     2761 tttggccgaa ggttatagag gattaccaga attgaataaa gaaaaatttg tgaacaactg
     2821 gtttgttgaa aaagatcact ggaattattt ggataaggat aatggtgaac cttggagaca
     2881 attctggtta ggtccaagag atagattgta cagaacgggt gatttaggtc gttatctacc
     2941 aaacggtgac tgtgaatgtt gcggtagggc tgatgatcaa gttaaaattc gtgggttcag
     3001 aatcgaatta ggagaaatag atacgcacat ttcccaacat ccattggtaa gagaaaacat
     3061 tactttagtt cgcaaaaatg ccgacaatga gccaacattg atcacattta tggtcccaag
     3121 atttgacaag ccagatgact tgtctaagtt ccaaagtgat gttccaaagg aggttgaaac
     3181 tgaccctata gttaagggct taatcggtta ccatctttta tccaaggaca tcaggacttt
     3241 cttaaagaaa agattggcta gctatgctat gccttccttg attgtggtta tggataaact
     3301 accattgaat ccaaatggta aagttgataa gcctaaactt caattcccaa ctcccaagca
     3361 attaaatttg gtagctgaaa atacagtttc tgaaactgac gactctcagt ttaccaatgt
     3421 tgagcgcgag gttagagact tatggttaag tatattacct accaagccag catctgtatc
     3481 accagatgat tcgtttttcg atttaggtgg tcattctatc ttggctacca aaatgatttt
     3541 taccttaaag aaaaagctgc aagttgattt accattgggc acaattttca agtatccaac
     3601 gataaaggcc tttgccgcgg aaattgacag aattaaatca tcgggtggat catctcaagg
     3661 tgaggtcgtc gaaaatgtca ctgcaaatta tgcggaagac gccaagaaat tggttgagac
     3721 gctaccaagt tcgtacccct ctcgagaata ttttgttgaa cctaatagtg ccgaaggaaa
     3781 aacaacaatt aatgtgtttg ttaccggtgt cacaggattt ctgggctcct acatccttgc
     3841 agatttgtta ggacgttctc caaagaacta cagtttcaaa gtgtttgccc acgtcagggc
     3901 caaggatgaa gaagctgcat ttgcaagatt acaaaaggca ggtatcacct atggtacttg
     3961 gaacgaaaaa tttgcctcaa atattaaagt tgtattaggc gatttatcta aaagccaatt
     4021 tggtctttca gatgagaagt ggatggattt ggcaaacaca gttgatataa ttatccataa
     4081 tggtgcgtta gttcactggg tttatccata tgccaaattg agggatccaa atgttatttc
     4141 aactatcaat gttatgagct tagccgccgt cggcaagcca aagttctttg actttgtttc
     4201 ctccacttct actcttgaca ctgaatacta ctttaatttg tcagataaac ttgttagcga
     4261 agggaagcca ggcattttag aatcagacga tttaatgaac tctgcaagcg ggctcactgg
     4321 tggatatggt cagtccaaat gggctgctga gtacatcatt agacgtgcag gtgaaagggg
     4381 cctacgtggg tgtattgtca gaccaggtta cgtaacaggt gcctctgcca atggttcttc
     4441 aaacacagat gatttcttat tgagattttt gaaaggttca gtccaattag gtaagattcc
     4501 agatatcgaa aattccgtga atatggttcc agtagatcat gttgctcgtg ttgttgttgc
     4561 tacgtctttg aatcctccca aagaaaatga attggccgtt gctcaagtaa cgggtcaccc
     4621 aagaatatta ttcaaagact acttgtatac tttacacgat tatggttacg atgtcgaaat
     4681 cgaaagctat tctaaatgga agaaatcatt ggaggcgtct gttattgaca ggaatgaaga
     4741 aaatgcgttg tatcctttgc tacacatggt cttagacaac ttacctgaaa gtaccaaagc
     4801 tccggaacta gacgatagga acgccgtggc atctttaaag aaagacaccg catggacagg
     4861 tgttgattgg tctaatggaa taggtgttac tccagaagag gttggtatat atattgcatt
     4921 tttaaacaag gttggatttt tacctccacc aactcataat gacaaacttc cactgccaag
     4981 tatagaacta actcaagcgc aaataagtct agttgcttca ggtgctggtg ctcgtggaag
     5041 ctccgcagca gcttaaggtt gagcattacg tatgatatgt ccatgtacaa taattaaata
     5101 tgaattagga gaaagactta gcttcttttc gggtgatgtc acttaaaaac tccgagaata
     5161 atatataata agagaataaa atattagtta ttgaataaga actgtaaatc agctggcgtt
     5221 agtctgctaa tggcagcttc atcttggttt attgtagcat gaatcatatt tgcctttttt
     5281 tcctgtaatt caatgattct tgcttctata ctatcctcaa tgcaaaacct tgtgatcttc
     5341 acaggtcgat actgaccaat tctatgaact ctatcaccac tttgccattc aacactaggg
     5401 ttccaccatg ggtctaaaat gaatacttgc gaagctgatc tatggtgcac tctcagtaca
     5461 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg
     5521 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     5581 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc
     5641 gtgatacgcc tatttttata ggttaatgtc atgatctgga tggcggcgtt agtatcgaat
     5701 cgacagcagt atagcgacca gcattcacat acgattgacg catgatatta ctttctgcgc
     5761 acttaacttc gcatctgggc agatgatgtc gaggcgaaaa aaaatataaa tcacgctaac
     5821 atttgattaa aatagaacaa ctacaatata aaaaaactat acaaatgaca agttcttgaa
     5881 aacaagaatc tttttattgt cagtactgat taggggcagg gcatgctcat gtagagcgcc
     5941 tgctcgccgt ccgaggcggt gccgtcgtac agggcggtgt ccaggccgca gagggtgaac
     6001 cccatccgcc ggtacgcgtg gatcgccggt gcgttgacgt tggtgacctc cagccagagg
     6061 tgcccggcgc cccgctcgcg ggcgaactcc gtcgcgagcc ccatcaacgc gcgcccgacc
     6121 ccgtgccccc ggtgctccgg ggcgacctcg atgtcctcga cggtcagccg gcggttccag
     6181 ccggagtacg agacgaccac gaagcccgcc aggtcgccgt cgtccccgta cgcgacgaac
     6241 gtccgggagt ccgggtcgcc gtcctccccg tcgtccgatt cgtcgtccga ttcgtcgtcg
     6301 gggaacacct tggtcagggg cgggtccacc ggcacctccc gcagggtgaa gccgtccccg
     6361 gtggcggtga cgcggaagac ggtgtcggtg gtgaaggacc catccagtgc ctcgatggcc
     6421 tcggcgtccc ccgggacact ggtgcggtac cggtaagccg tgtcgtcaag agtggtaccc
     6481 atggttgttt atgttcggat gtgatgtgag aactgtatcc tagcaagatt ttaaaaggaa
     6541 gtatatgaaa gaagaacctc agtggcaaat cctaaccttt tatatttctc tacaggggcg
     6601 cggcgtgggg acaattcaac gcgtctgtga ggggagcgtt tccctgctcg caggtctgca
     6661 gcgaggagcc gtaatttttg cttcgcgccg tgcggccatc aaaatgtatg gatgcaaatg
     6721 attatacatg gggatgtatg ggctaaatgt acgggcgaca gtcacatcat gcccctgagc
     6781 tgcgcacgtc aagactgtca aggagggtat tctgggcctc catgtcgctg gccgggtgac
     6841 ccggcgggga cgaggcaagc taaacaagac gtcaggtggc acttttcggg gaaatgtgcg
     6901 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     6961 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7021 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7081 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7141 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7201 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7261 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7321 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7381 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7441 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7501 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7561 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7621 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7681 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7741 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     7801 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     7861 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     7921 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     7981 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8041 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8101 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8161 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8221 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8281 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8341 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8401 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8461 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8521 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8581 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8641 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     8701 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     8761 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     8821 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     8881 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     8941 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     9001 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta
     9061 aagggaacaa aagctggagc tccaccgcgg tggcggccgc gccacctggg tccttttcat
     9121 cacgtgctat aaaaataatt ataatttaaa ttttttaata taaatatata aattaaaaat
     9181 agaaagtaaa aaaagaaatt aaagaaaaaa tagtttttgt tttccgaaga tgtaaaagac
     9241 tctaggggga tcgccaacaa atactacctt ttatcttgct cttcctgctc tcaggtatta
     9301 atgccgaatt gtttcatctt gtctgtgtag aagaccacac acgaaaatcc tgtgatttta
     9361 cattttactt atcgttaatc gaatgtatat ctatttaatc tgcttttctt gtctaataaa
     9421 tatatatgta aagtacgctt tttgttgaaa ttttttaaac ctttgtttat ttttttttct
     9481 tcattccgta actcttctac cttctttatt tactttctaa aatccaaata caaaacataa
     9541 aaataaataa acacagagta aattcccaaa ttattccatc attaaaagat acgaggcgcg
     9601 tgtaagttac aggcaagcga tccgtcctaa gaagcgctaa gagggcggcc gctctagaac
     9661 tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga
     9721 gggggggccc ggtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     9781 tcgttttaca acgtcgtgac tggg
//
