LOCUS       sequence-238765-       15984 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targets ptena and ptenb and expresses zebrafish mitfa specifically 
            in zebrafish melanocytes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15984)
  AUTHORS   Ablain J, Xu M, Rothschild H, Jordan RC, Mito JK, Daniels BH, Bell 
            CF, Joseph NM, Wu H, Bastian BC, Zon LI, Yeh I
  TITLE     Human tumor genomics and zebrafish modeling identify SPRED1 loss as 
            a driver of mucosal melanoma.
  JOURNAL   Science. 2018 Nov 1. pii: science.aau6509. doi: 
            10.1126/science.aau6509.
  PUBMED    30385465
REFERENCE   2  (bases 1 to 15984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2018 Nov 1. pii: science.aau6509. doi: 10.1126/science.aau6509."
COMMENT     Sequence Label: MiniCoopR 2xU6:gRNA pten, mitfa:Cas9
FEATURES             Location/Qualifiers
     source          1..15984
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(125..193,194..985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(343..362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1156..1744
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1645..1664
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1898..1915
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2032..2053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2068..2073,2074..2091,2092..2098)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2106..2122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2111..2133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2130..2146
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2167..2185
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     2222..2238
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2222..2238
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(2272..2283,2284..2471)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     polyA_signal    7262..7396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_RNA        7781..7856
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        8251..8326
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     8339..8355
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8363..8383
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    8446..8580
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8582..8606
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        8635..8653
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             complement(8707..8727)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(8743..12843)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes MGAS15252 Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDDYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFGSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLADSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQIYNQLFEENPINASRVDAKAILSARLSKSRRL
                     ENLIAQLPGEKRNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNSEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGAYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDRGMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGHSLHEQIANLAGSPAIKKGILQTVKIVDELVKVMGH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFIKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8822..8830
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(12850..12870)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      12879..12888
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(12923..12942)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(12924..12942)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(12952..12968)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(12970..12994)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(14868..14888)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(14908..14924)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    join(15297..15459,15460..15471)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     15820..15841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
       61 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
      121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
      181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
      241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
      301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
      361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
      421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
      481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
      541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
      601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
      661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
      721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
      781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
      841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
      901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     1021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1081 catgaccaaa atgccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1141 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1201 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1261 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     1321 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1381 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1441 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1501 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1561 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1621 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1681 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1741 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     1801 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     1861 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     1921 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     1981 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     2041 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     2101 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     2161 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg gtggcggccg
     2221 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
     2281 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
     2341 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
     2401 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
     2461 ggagatcact tcattctatt ttcccttgct attaccaaac caattgaatt gcgctgatgc
     2521 ccagtttaat ttaaatgtta tttattctgc ctatgaaaat cgttttcaca ttatatgaaa
     2581 ttggtcagac atgttcattg gtcctttgga agtgacgtca tgtcacatct attaccacaa
     2641 tgcacagcac cttgacctgg aaattaggga aattataaca gtcaatcagt ggaagaaaat
     2701 ggaggaagta tgtgattcat cagcagctgc gagcagcaca gtccaaaatc agccacagga
     2761 tcaagagcac ccgtggccgt atcttcgcag atctcgaata agagattgct acggagagat
     2821 attctgctga ttaggagctt gtctatgcta atttggtttt gttcaattca cctatgtcgc
     2881 aagtttttgg actgtaggag aaaaccggag aacccagagg atatccacat gagcacgagg
     2941 acaacatgta aactccacac agaaccatgg actggcctgt tagagacttg gaccagtgac
     3001 gttcttgctt tgaggcaaca gtgctaacaa ctgggctgct gtgccgctct ttcgaggaaa
     3061 tgaaaggaac tctggcggcc tatgatgagt taggaattgt ctgattggtg aatcatacat
     3121 aggctaatgt gggaccagct gtagtcagtc ataagcacgt gattctctcg aaattagttt
     3181 ataaataaac ttcactaaac tccaaccgtt cattctgttt tagatgttgc ctttaaactt
     3241 gccttaagat gttaataaag ttgctttttg gttgttgaca aagctaggca taggacgata
     3301 accgttttca aggtataccc cggtttgaaa aagtcaaggg tttaaaacca ccaatatttt
     3361 ctgctatacc gttcctaagg tatatttaag attttttatt tattttttat ttttttgttt
     3421 tttaggacaa cagcattttc agcagaaaag agatacaaaa atgcttttta aattgtaaag
     3481 aaatctgttt ttgaaactaa tgaagacagc agaagtcgat cattcattga aattattaag
     3541 cttgacatgt tttctgttcc aaaatatttt ttcaatgttt ctcaaaataa aatatattat
     3601 gtttaaagtt tttgtttttt actaagacat taaaaaaaat aatatatttt agagtggcaa
     3661 tcacaatacc atcaaactgt gatattttta tcaaaggtta tcataccatc agaatcttat
     3721 accccatgcc tagacacagc tgttctctct atctctctat gttaatgcag tttccactcc
     3781 attgacgaga tggttctctg taccacatac tcatccccat tttacaatag ctacatttgc
     3841 tcaagatgta gtcaactcaa acaatgcaga gacaacaaac tgttgactta tcttttctat
     3901 cgtaattcag cccttttggg aaatgagcat caacacacaa tttagagtgg aagttgggtt
     3961 aaggcagact cattttttac acattacaac cagtgattat gtcaattatt atttaaaaaa
     4021 aattcagtac agtatatgca catgctactt tgtttatgat tgttagcttt tttaactaca
     4081 gttcatttct atgctttctg tgcaaatgca caacagttac tgatttactc tgatgataat
     4141 tgtgccagta catccaacaa caacaaaaaa aaaaaaaaga cttacaatca agccccaaag
     4201 tgttcatacg tctggcaaat tctgacttaa agttactttt attcaaccag ctagtttttt
     4261 tttgactgta aatgacacgt aagataataa gatgatgtac atgaggcatc attgtagaaa
     4321 aaaaaatgaa tatgaatctg aatatgaact gtacgtctgt gattttgttt gtgcgcactt
     4381 acatacgtgt gcacaatatt tcaactctta agaaaaaaat gcaattacca aaggcccatc
     4441 agacaacaaa gttctctttc ctatcttttg ctcttcaaca tttcagagat aactccatct
     4501 taaactgtct tgtttttaga ccaacagtgc tagtgattgt atgccggatg ctgagaacaa
     4561 acaatgtttt atgcaggatg agtggaacta aagatgaaac gttagttagc cgtaagccag
     4621 tttcagttta gacactagta acccatcgtc tcaaagaggc ttttgtcgaa tcggaccggt
     4681 gacgtcaaat gaactttata aaggcataaa ttcatcgttt gggtaaaaaa aacaatatga
     4741 gaagagcaat cccctcccac ccccttaaat acattttgtg tcttttcttt ggcattgaca
     4801 tcttcagctg gccaagacga ctggtcagtt cttgcacatc acctaaattc acaaagggaa
     4861 tacttttttg aagctaacac atagttgaac atgttggaga tgctcgagta cagtcactac
     4921 caggttcaga cccacctgga aaccccctcg aagtaccaca tccagcagag ccagaggcag
     4981 caagtgaagc actacctgtc cagtgcactg ggagccaagc tgagcccaca ggccagcaca
     5041 gggcccggcc ccagccagcc cgccgagcac ggcatgaccc cgggacccgg agccagcgct
     5101 cccaacagcc ctatggccct tctcaccctc aactgtgaga aagagatgga cgatgtaatt
     5161 gaagacatta taagtttgga atcaagctac agtgatgaca ttcttgggtt catggatgca
     5221 ggacttcaaa tgacaaacac gattccagtt tcagcaaacc tgctggacat gtacagcaat
     5281 catgctcttc ctccagctgg agtttccatc agtaactcct gcccctcaag cctgccggcc
     5341 gtcaaaaggg aattatccgt tactccatct ccgggcatga tgcacataat ggacaaagct
     5401 ggaccatgtg gcaagtttga ctcttatcaa agacctgatg gctttccagt agaagcagaa
     5461 gtcagagccc tggcaaaaga gagacaaaag aaggacaacc acaacctcat tgaacgaaga
     5521 aggcggttta atatcaatga cagaattaag gagctgggga ctttaattcc caagtcaaat
     5581 gatccagaca tgaggtggaa taaaggcacc atcctgaaag catcagtgga ttacattagg
     5641 aaattgcaga aagagcagca gaaagcaaaa gagctggaaa acagacagaa gagactagaa
     5701 cacgcaaaca gacatctcct gctccgtatt caggaacttg aaatgcaagc ccgtgctcat
     5761 ggactcaccg ttgtagcttc ttccagcctt tattccgctg agttagtggc tcgagcaatc
     5821 aaacaagagc ctgggatggg ggactgcaca tccaatttgt acccacacct tcccagtcct
     5881 gacatgtccc gtcctaccac tctggacctc aacaacggca ccatcagcta caatgacagt
     5941 cccacggagg acggcgagcc gggggtctac gacagcccaa acaaggcctc caccaagctg
     6001 gaggacatgc ttatggacaa caccctgtct ccggtgggca gcagcgaccc gctgttgtcc
     6061 tccggatccc cagtaccttc caacagtagc ggcagcagca gtatggacga acatgacaat
     6121 ggctgttagc aatgccaact aaatttcatg aaccaaagtt agacaattag atttgtgtgt
     6181 gtgtgtgtct tgtaagtacc agtgttacat ccagatcatg aagagcgcaa tttcgtaaag
     6241 tattaatctt gtatttcaga atatttagtg tgcttgtgta tatatttttt catttatcat
     6301 gtcagagtct agtcaagaat gaaatcgtgt aaacaattag ttgatttttg aaggattctc
     6361 gcggatgata taaagagtag cttgaataaa aactgttgag gtgtctttta tttgcaggct
     6421 tggcttattg ttgtcctcaa atgcagctcc atttcttggg gtgtacatct tgcaagggtg
     6481 gaggacatgt ctaggcatgt acaactggat tcactttcat tcagtcattc attttctttt
     6541 tggcttagcc cctttattaa tcaggggttg ccacagttga atgaactgcc aacttatgcc
     6601 aacaaacaaa tccagcggat gcccttgcag ctgcaaccca tcactgggaa acacccacca
     6661 cagtaccatt cactttagac aatttagctt acccaattca cctatagcgc atgtctttgg
     6721 gctgtaggtg aaactggagc acccggagga aacccacacg aacatgggga gaacatgcaa
     6781 actccacaca gaaatgaccc aaccaagtct cgaaccagcg accttcttgc tgtaaggcga
     6841 caatgctacc cactgcacca ccacgatgcc ctggaaaaac tttttacatt gttatttaac
     6901 attaagtcca aaagtatttc tgcagtattt ctgaaatttg ttttattttc tagtggaaga
     6961 gctgtgatgc aagttctcca taataattag aaaaaaaatg cccaaattgc tttgatttta
     7021 gggatagttc acccaaaaat gaattttttt ttattaatta atcatcctca tgtcgttcca
     7081 attccctgag acctttgttc atctttaaaa cacaaaaaat gatattttgg atacaatctg
     7141 agatctgatc tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7201 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7261 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7321 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7381 ttatcatgtc tggatcatca tcgatgcgtc ttttgttctg gtcatcaagg aggggggaat
     7441 gttctgcgca tgcctgtggg ggagggagaa ggacacgtca ctgaaaacgt ccctgcatca
     7501 caccgagaca cccaatcact caagccgaga ccagataatt ttgcatatgc tttaaagttt
     7561 gaaaaatacc acggtaaacc ctcacacaaa ctctggattt gagatctttc aggttttatc
     7621 agtttgcagg tttatgtcac catgatatag ggtcagactt gatctaaggg agctgaataa
     7681 gtggtttagt cactcaccac ctcccaaaaa catacccaga agtccctggt atatatagct
     7741 ctccctccag ctcttggttc gaataagcgg aggtaccagg gttttagagc tagaaatagc
     7801 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     7861 ttggtcgacc tcgaggcgtc ttttgttctg gtcatcaagg aggggggaat gttctgcgca
     7921 tgcctgtggg ggagggagaa ggacacgtca ctgaaaacgt ccctgcatca caccgagaca
     7981 cccaatcact caagccgaga ccagataatt ttgcatatgc tttaaagttt gaaaaatacc
     8041 acggtaaacc ctcacacaaa ctctggattt gagatctttc aggttttatc agtttgcagg
     8101 tttatgtcac catgatatag ggtcagactt gatctaaggg agctgaataa gtggtttagt
     8161 cactcaccac ctcccaaaaa catacccaga agtccctggt atatatagct ctccctccag
     8221 ctcttggttc gagacagtgc ctatgttcag gttttagagc tagaaatagc aagttaaaat
     8281 aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttggtaccgt
     8341 aaaacgacgg ccagtgaatt atcaactatg tataataaag ttggaaaaaa cctcccacac
     8401 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     8461 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8521 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     8581 ccccactttg tacaagaaag ctgggtgcta tgaccatgat tacgccaagc gcgcaattaa
     8641 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagattccg
     8701 cggtcacacc tttctcttct tcttaggaga tctcaccggt gagtcgccac ccagctgaga
     8761 caggtcaatc cttgtttcgt acaggccggt aatactctga tggatcagtg tagcgtccag
     8821 cacctcctta gtggaggtgt acctctttct atcgattgta gtgtcgaagt acttgaaagc
     8881 tgctggtgcg cccaggtttg tcagagtgaa cagatgaatg atattttcgg cctgctccct
     8941 gatgggctta tctctgtgtt tgttgtaagc agacaggacc ttatccagat ttgcgtcggc
     9001 caggatcact cttttagaga actcgctaat ctgttcaatg atctcgtcca gataatgctt
     9061 gtgctgctcc acaaacagct gtttctgttc gttatcctct ggagatcctt tcagcttctc
     9121 gtagtggctt gccagataca ggaagttcac gtacttagaa ggcagggcca gttcattgcc
     9181 tttctgcagc tctccagcgc ttgccagcat gcgtttcctg ccgttttcca gctcaaacag
     9241 actgtacttg ggcagtttaa tgatcaggtc tttcttcact tccttataac ctttggcctc
     9301 cagaaaatcg attgggttct tctcgaaaga gcttctctcc ataattgtga tacccagcag
     9361 ctccttgaca gatttcagtt tcttgctttt gcccttctcc accttagcga ccaccaggac
     9421 ggagtatgcc acagtgggag agtcaaagcc tccatacttc ttagggtccc agtctttctt
     9481 tctagcgatc agcttatcag agttgcgttt tggcagaata ctttcttttg agaaaccgcc
     9541 tgtctgcacc tcagttttct tgacgatatt cacttgaggc atgctcagga ccttcctcac
     9601 ggttgcaaaa tctctgcctt tgtcccacac gatttcgcct gtctctccgt ttgtctcaat
     9661 caggggcctt ttgcggattt ctccattggc cagggtgatc tctgtcttaa agaaattcat
     9721 gatgtttgag taaaagaaat acttggcagt agcttttcca atttcctgct cggacttggc
     9781 gatcattttt ctgacatcgt acaccttata gtctccgtac acgaattcgc tctccagttt
     9841 tgggtatttc ttgatcagtg cggtaccgac cacggcgttc aggtatgcgt catgggcatg
     9901 gtgataattg ttaatctcgc ggaccttata aaactggaaa tctttcctaa agtcgctgac
     9961 cagtttactc ttcagggtaa tgaccttcac ttctctgatc agtttgtcat tctcatcgta
    10021 ctttgtgttc atgcgactat ccagaatctg tgcgacgtgt ttagtgatct ggcgtgtttc
    10081 caccagctgc ctcttgatga atccagcttt gtccagttct gacagtccac cgcgctctgc
    10141 ttttgtcagg ttatcaaact tcctctgagt gatcagtttg gcattcagca gctgtctcca
    10201 gtagttcttc attttcttga ccacttcctc actagggaca ttatctgatt ttcctctgtt
    10261 tttgtcggac ctggtcagca ccttgttgtc aatgctgtca tctttgatga aactctgggg
    10321 gacgatatga tccacgtcgt aatcagacag cctgttaata tccagctcct ggtccacgta
    10381 catatctctt ccattctgca gatagtacag atacagcttc tcattctgca gctgtgtgtt
    10441 ttccactggg tgctctttca ggatctggct acccagctcc ttaatgcctt cctcgattct
    10501 tttcatcctc tctctactgt tcttctgacc cttctgggtt gtctgatttt cgcgagccat
    10561 ctcaatcacg atgttttcgg gcttatggcc catgactttc accagctcat cgacgatttt
    10621 cactgtctgc agaatgcctt tcttgatggc tgggcttcca gccaggtttg caatctgctc
    10681 atgcagggag tggccctgtc cagacacctg agccttctgg atgtcctctt tgaaggtcag
    10741 actgtcatcg tgaatcagct gcataaaatt gcggttggcg aagccatcac ttttcagaaa
    10801 gtccaggatt gtctttcctg actgtttatc cctaatgccg ttgatcagct tcctgctcag
    10861 tctaccccag ccagtgtagc gccttctctt cagctgtttc atcaccttgt catcgaacag
    10921 atgagcatag gttttcagtc tttcctcgat cattcctctg tcctcaaaca gggtcagtgt
    10981 cagcacaatg tcttccagga tatcctcatt ttcctcgttg tccaggaagt ctttatcctt
    11041 aatgatcttc agcagatcgt ggtaagcgcc caggcttgcg ttaaatctgt cttcgactcc
    11101 gctgatctcc acggaatcga agcattcaat tttcttgaag tagtcctcct tcagctgctt
    11161 gacagtcact ttcctgttgg ttttaaacag cagatccacg attgctttct tctgctctcc
    11221 tgacaggaag gctggctttc tcatgccctc ggtgacgtat ttcacctttg tcagttcgtt
    11281 atagacagtg aaatactcgt acagcaggga gtgcttaggc agcactttct cattgggcag
    11341 gtttttgtca aaattagtca ttctctcgat gaaggactgt gcagaggcgc ccttatcgac
    11401 cacttcctcg aagttccaag gagtaatggt ttcctcggat ttgcgagtca tccaggcaaa
    11461 tctgctgttt ccgcgagcca gtggacccac atagtaaggg attctgaagg tcagaatttt
    11521 ttcgatcttc tcgcggttat ctttcagaaa ggggtagaag tcttcctggc gcctcaggat
    11581 agcgtgcagc tctcccagat gaatctggtg tgggatggaa ccgttatcaa atgtgcgctg
    11641 cttcctcagc aggtcctctc tattcagttt caccagcagt tcctcagttc cgtccatctt
    11701 ctccagaata ggtttgataa acttgtagaa ttcctcctgt gatgcgcctc cgtcgatata
    11761 accggcgtag ccgttcttgg actgatcaaa gaaaatttcc ttgtacttct ctggcagctg
    11821 ctgcctgacc agagccttca gcagggtcag atcctgatgg tgctcgtcat atctcttgat
    11881 catgctggca ctcagaggtg cttttgtaat ctcgctgttc actctcagaa tatctgacag
    11941 caggattgcg tcggacagat ttttggcagc caggaacagg tcagcgtact gatctccgat
    12001 ctgtgccagc aggttatcca ggtcatcgtc atatgtgtcc tttgacagct gcagtttggc
    12061 gtcctcagcc agatcaaaat tgcttttgaa gttaggagtc aggcccagac tcagagcgat
    12121 cagatttcca aacagaccgt tcctcttttc tccgggcagc tgtgcaatca ggttctccag
    12181 tctgcgtgac ttggacagcc tggctgacag gatagctttt gcgtccactc tggaagcatt
    12241 aatagggttt tcctcaaaca gctgattgta aatctggacc agctggatga acagcttgtc
    12301 cacatcggaa ttgtcggggt tcagatcgcc ctcgatcaga aaatgacccc tgaacttaat
    12361 catgtgggcc agagccagat agatcagtct caggtcggct ttatcagtag agtcagccag
    12421 tttcttgcgc agatgataga tggttgggta tttttcgtga taggcgacct cgtccacaat
    12481 gtttccaaag ataggatggc gctcgtgttt cttatcttcc tcgaccagga aactttcctc
    12541 cagcctatga aagaatgaat cgtccacctt agccatttcg ttagagaaaa tctcctgcag
    12601 gtaacagatt ctattcttcc ttctggtata gcgccttctt gctgttcttt tcaggcgtgt
    12661 tgcttcggca gtctctccag aaccgaacag cagggctcca atcagatttt tcttgatgct
    12721 gtgcctgtcg gtgttaccca gcaccttaaa tttcttgcta gggaccttgt agtcgtctgt
    12781 aatcactgcc caacccacgg agttagttcc aatatccagg ccgatgctat acttcttatc
    12841 catgcttccc acctttctct tcttcttagg tggagaagcc atggtggcgt cgacctcgag
    12901 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    12961 cgttttacaa gcctgctttt ttgtacaaac ttgcgttcaa ctatgtgtta gcttcaaaaa
    13021 agtattccct ttgtgaattt aggtgatgtg caagaactga ccagtcgtct tggccagctg
    13081 aagatgtcaa tgccaaagaa aagacacaaa atgtatttaa gggggtggga ggggattgct
    13141 cttctcatat tgtttttttt acccaaacga tgaatttatg cctttataaa gttcatttga
    13201 cgtcaccggt ccgattcgac aaaagcctct ttgagacgat gggttactag tgtctaaact
    13261 gaaactggct tacggctaac taacgtttca tctttagttc cactcatcct gcataaaaca
    13321 ttgtttgttc tcagcatccg gcatacaatc actagcactg ttggtctaaa aacaagacag
    13381 tttaagatgg agttatctct gaaatgttga agagcaaaag ataggaaaga gaactttgtt
    13441 gtctgatggg cctttggtaa ttgcattttt ttcttaagag ttgaaatatt gtgcacacgt
    13501 atgtaagtgc gcacaaacaa aatcacagac gtacagttca tattcagatt catattcatt
    13561 tttttttcta caatgatgcc tcatgtacat catcttatta tcttacgtgt catttacagt
    13621 caaaaaaaaa ctagctggtt gaataaaagt aactttaagt cagaatttgc cagacgtatg
    13681 aacactttgg ggcttgattg taagtctttt tttttttttt gttgttgttg gatgtactgg
    13741 cacaattatc atcagagtaa atcagtaact gttgtgcatt tgcacagaaa gcatagaaat
    13801 gaactgtagt taaaaaagct aacaatcata aacaaagtag catgtgcata tactgtactg
    13861 aatttttttt aaataataat tgacataatc actggttgta atgtgtaaaa aatgagtctg
    13921 ccttaaccca acttccactc taaattgtgt gttgatgctc atttcccaaa agggctgaat
    13981 tacgatagaa aagataagtc aacagtttgt tgtctctgca ttgtttgagt tgactacatc
    14041 ttgagcaaat gtagctattg taaaatgggg atgagtatgt ggtacagaga accatctcgt
    14101 caatggagtg gaaactgcat taacatagag agatagagag aacagctgtg tctaggcatg
    14161 gggtataaga ttctgatggt atgataacct ttgataaaaa tatcacagtt tgatggtatt
    14221 gtgattgcca ctctaaaata tattattttt tttaatgtct tagtaaaaaa caaaaacttt
    14281 aaacataata tattttattt tgagaaacat tgaaaaaata ttttggaaca gaaaacatgt
    14341 caagcttaat aatttcaatg aatgatcgac ttctgctgtc ttcattagtt tcaaaaacag
    14401 atttctttac aatttaaaaa gcatttttgt atctcttttc tgctgaaaat gctgttgtcc
    14461 taaaaaacaa aaaaataaaa aataaataaa aaatcttaaa tataccttag gaacggtata
    14521 gcagaaaata ttggtggttt taaacccttg actttttcaa accggggtat accttgaaaa
    14581 cggttatcgt cctatgccta gctttgtcaa caaccaaaaa gcaactttat taacatctta
    14641 aggcaagttt aaaggcaaca tctaaaacag aatgaacggt tggagtttag tgaagtttat
    14701 ttataaacta atttcgagag aatcacgtgc ttatgactga ctacagctgg tcccacatta
    14761 gcctatgtat gattcaccaa tcagacaatt cctaactcat cataggccgc cagagttcct
    14821 ttcatttcct cgaaagagcg gcacagcagc ccagttgtta gcactcacaa cttttctata
    14881 caaagttgat agcttggcgt aatcatggtc atagctgttt cctgctcgag ggcccatctg
    14941 gcctgtgttt cagacaccag ggagtctctg ctcacgtttc ctgctatttg cagcctctct
    15001 atcaagacta atacacctct tcccgcatcg gctgcctgtg agaggctttt cagcactgca
    15061 ggattgcttt tcagccccaa aagagctagg cttgacacta acaattttga gaatcagctt
    15121 ctactgaagt taaatctgag gttttacaac tttgagtagc gtgtactggc attagattgt
    15181 ctgtcttata gtttgataat taaatacaaa cagttctaaa gcaggataaa accttgtatg
    15241 catttcattt aatgtttttt gagattaaaa gcttaaacaa gaatctctag ttttctttct
    15301 tgcttttact tttacttcct taatactcaa gtacaatttt aatggagtac ttttttactt
    15361 ttactcaagt aagattctag ccagatactt ttacttttaa ttgagtaaaa ttttccctaa
    15421 gtacttgtac tttcacttga gtaaaatttt tgagtacttt ttacacctct gtcaagaact
    15481 cctggacaaa cctctgacct gtgtggaaca gagtggatat gggtgtctga acagatattc
    15541 acgtcttttg cagatcagag ggcatttctg gtgttgtgca atgacctggg tccaacaggt
    15601 aggcgtactc gtatccaaga gcattgttgg acaggaggta gtctccattc ctataaagag
    15661 gaaggaatgg caccatgtag tagccgtcat tgtggccaat tggtgcattg gtacgtgggt
    15721 agatggaccg gggaggctga tgagttctta gccagcgtgg gccatcgccc tgatagacgg
    15781 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    15841 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    15901 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    15961 tattaacgct tacaatttag gtgg
//
