LOCUS       sequence-238766-        9151 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-URA3 integrating (YIp) vector for stable genomic integration in
            the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9151)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-URA3
FEATURES             Location/Qualifiers
     source          1..9151
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        123..362
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             363..4541
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(4942..4961)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5061..5083
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5121..5139)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5178..5393
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             5394..6197
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        6283..6387
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6388..6456,6457..7248)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6606..6625)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7419..8007
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7908..7927
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8161..8178
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8295..8316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8331..8336,8337..8354,8355..8361)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8369..8385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8374..8396
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8393..8409
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8393..8409
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8405..8785
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     8428..8448
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8430..8448
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8461..8568
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     8485..8501
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8485..8501
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8535..8551)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8536..8552)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8576..8595)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8577..8595)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8605..8622)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8605..8621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8614..8636)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8762..9151,1..66)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8849..8868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9059..9080
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
       61 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcataga
      121 tcaattccac ttgcaattac ataaaaaatt ccggcggttt ttcgcgtgtg actcaatgtc
      181 gaaatacctg cctaatgaac atgaacatcg cccaaatgta tttgaagacc cgctgggaga
      241 agttcaagat atataagtaa caagcagcca atagtataaa aaaaaatctg agtttattac
      301 ctttcctgga atttcagtga aaaactgcta attatagaga gatatcacag agttactcac
      361 taatgactaa cgaaaaggtc tggatagaga agttggataa tccaactctt tcagtgttac
      421 cacatgactt tttacgccca caacaagaac cttatacgaa acaagctaca tattcgttac
      481 agctacctca gctcgatgtg cctcatgata gtttttctaa caaatacgct gtcgctttga
      541 gtgtatgggc tgcattgata tatagagtaa ccggtgacga tgatattgtt ctttatattg
      601 cgaataacaa aatcttaaga ttcaatattc aaccaacgtg gtcatttaat gagctgtatt
      661 ctacaattaa caatgagttg aacaagctca attctattga ggccaatttt tcctttgacg
      721 agctagctga aaaaattcaa agttgccaag atctggaaag gacccctcag ttgttccgtt
      781 tggccttttt ggaaaaccaa gatttcaaat tagacgagtt caagcatcat ttagtggact
      841 ttgctttgaa tttggatacc agtaataatg cgcatgtttt gaacttaatt tataacagct
      901 tactgtattc gaatgaaaga gtaaccattg ttgcggacca atttactcaa tatttgactg
      961 ctgcgctaag cgatccatcc aattgcataa ctaaaatctc tctgatcacc gcatcatcca
     1021 aggatagttt acctgatcca actaagaact tgggctggtg cgatttcgtg gggtgtattc
     1081 acgacatttt ccaggacaat gctgaagcct tcccagagag aacctgtgtt gtggagactc
     1141 caacactaaa ttccgacaag tcccgttctt tcacttatcg cgacatcaac cgcacttcta
     1201 acatagttgc ccattatttg attaaaacag gtatcaaaag aggtgatgta gtgatgatct
     1261 attcttctag gggtgtggat ttgatggtat gtgtgatggg tgtcttgaaa gccggcgcaa
     1321 ccttttcagt tatcgaccct gcatatcccc cagccagaca aaccatttac ttaggtgttg
     1381 ctaaaccacg tgggttgatt gttattagag ctgctggaca attggatcaa ctagtagaag
     1441 attacatcaa tgatgaattg gagattgttt caagaatcaa ttccatcgct attcaagaaa
     1501 atggtaccat tgaaggtggc aaattggaca atggcgagga tgttttggct ccatatgatc
     1561 actacaaaga caccagaaca ggtgttgtag ttggaccaga ttccaaccca accctatctt
     1621 tcacatctgg ttccgaaggt attcctaagg gtgttcttgg tagacatttt tccttggctt
     1681 attatttcaa ttggatgtcc aaaaggttca acttaacaga aaatgataaa ttcacaatgc
     1741 tgagcggtat tgcacatgat ccaattcaaa gagatatgtt tacaccatta tttttaggtg
     1801 cccaattgta tgtccctact caagatgata ttggtacacc gggccgttta gcggaatgga
     1861 tgagtaagta tggttgcaca gttacccatt taacacctgc catgggtcaa ttacttactg
     1921 cccaagctac tacaccattc cctaagttac atcatgcgtt ctttgtgggt gacattttaa
     1981 caaaacgtga ttgtctgagg ttacaaacct tggcagaaaa ttgccgtatt gttaatatgt
     2041 acggtaccac tgaaacacag cgtgcagttt cttatttcga agttaaatca aaaaatgacg
     2101 atccaaactt tttgaaaaaa ttgaaagatg tcatgcctgc tggtaaaggt atgttgaacg
     2161 ttcagctact agttgttaac aggaacgatc gtactcaaat atgtggtatt ggcgaaatag
     2221 gtgagattta tgttcgtgca ggtggtttgg ccgaaggtta tagaggatta ccagaattga
     2281 ataaagaaaa atttgtgaac aactggtttg ttgaaaaaga tcactggaat tatttggata
     2341 aggataatgg tgaaccttgg agacaattct ggttaggtcc aagagataga ttgtacagaa
     2401 cgggtgattt aggtcgttat ctaccaaacg gtgactgtga atgttgcggt agggctgatg
     2461 atcaagttaa aattcgtggg ttcagaatcg aattaggaga aatagatacg cacatttccc
     2521 aacatccatt ggtaagagaa aacattactt tagttcgcaa aaatgccgac aatgagccaa
     2581 cattgatcac atttatggtc ccaagatttg acaagccaga tgacttgtct aagttccaaa
     2641 gtgatgttcc aaaggaggtt gaaactgacc ctatagttaa gggcttaatc ggttaccatc
     2701 ttttatccaa ggacatcagg actttcttaa agaaaagatt ggctagctat gctatgcctt
     2761 ccttgattgt ggttatggat aaactaccat tgaatccaaa tggtaaagtt gataagccta
     2821 aacttcaatt cccaactccc aagcaattaa atttggtagc tgaaaataca gtttctgaaa
     2881 ctgacgactc tcagtttacc aatgttgagc gcgaggttag agacttatgg ttaagtatat
     2941 tacctaccaa gccagcatct gtatcaccag atgattcgtt tttcgattta ggtggtcatt
     3001 ctatcttggc taccaaaatg atttttacct taaagaaaaa gctgcaagtt gatttaccat
     3061 tgggcacaat tttcaagtat ccaacgataa aggcctttgc cgcggaaatt gacagaatta
     3121 aatcatcggg tggatcatct caaggtgagg tcgtcgaaaa tgtcactgca aattatgcgg
     3181 aagacgccaa gaaattggtt gagacgctac caagttcgta cccctctcga gaatattttg
     3241 ttgaacctaa tagtgccgaa ggaaaaacaa caattaatgt gtttgttacc ggtgtcacag
     3301 gatttctggg ctcctacatc cttgcagatt tgttaggacg ttctccaaag aactacagtt
     3361 tcaaagtgtt tgcccacgtc agggccaagg atgaagaagc tgcatttgca agattacaaa
     3421 aggcaggtat cacctatggt acttggaacg aaaaatttgc ctcaaatatt aaagttgtat
     3481 taggcgattt atctaaaagc caatttggtc tttcagatga gaagtggatg gatttggcaa
     3541 acacagttga tataattatc cataatggtg cgttagttca ctgggtttat ccatatgcca
     3601 aattgaggga tccaaatgtt atttcaacta tcaatgttat gagcttagcc gccgtcggca
     3661 agccaaagtt ctttgacttt gtttcctcca cttctactct tgacactgaa tactacttta
     3721 atttgtcaga taaacttgtt agcgaaggga agccaggcat tttagaatca gacgatttaa
     3781 tgaactctgc aagcgggctc actggtggat atggtcagtc caaatgggct gctgagtaca
     3841 tcattagacg tgcaggtgaa aggggcctac gtgggtgtat tgtcagacca ggttacgtaa
     3901 caggtgcctc tgccaatggt tcttcaaaca cagatgattt cttattgaga tttttgaaag
     3961 gttcagtcca attaggtaag attccagata tcgaaaattc cgtgaatatg gttccagtag
     4021 atcatgttgc tcgtgttgtt gttgctacgt ctttgaatcc tcccaaagaa aatgaattgg
     4081 ccgttgctca agtaacgggt cacccaagaa tattattcaa agactacttg tatactttac
     4141 acgattatgg ttacgatgtc gaaatcgaaa gctattctaa atggaagaaa tcattggagg
     4201 cgtctgttat tgacaggaat gaagaaaatg cgttgtatcc tttgctacac atggtcttag
     4261 acaacttacc tgaaagtacc aaagctccgg aactagacga taggaacgcc gtggcatctt
     4321 taaagaaaga caccgcatgg acaggtgttg attggtctaa tggaataggt gttactccag
     4381 aagaggttgg tatatatatt gcatttttaa acaaggttgg atttttacct ccaccaactc
     4441 ataatgacaa acttccactg ccaagtatag aactaactca agcgcaaata agtctagttg
     4501 cttcaggtgc tggtgctcgt ggaagctccg cagcagctta aggttgagca ttacgtatga
     4561 tatgtccatg tacaataatt aaatatgaat taggagaaag acttagcttc ttttcgggtg
     4621 atgtcactta aaaactccga gaataatata taataagaga ataaaatatt agttattgaa
     4681 taagaactgt aaatcagctg gcgttagtct gctaatggca gcttcatctt ggtttattgt
     4741 agcatgaatc atatttgcct ttttttcctg taattcaatg attcttgctt ctatactatc
     4801 ctcaatgcaa aaccttgtga tcttcacagg tcgatactga ccaattctat gaactctatc
     4861 accactttgc cattcaacac tagggttcca ccatgggtct aaaatgaata cttgcgaagc
     4921 tgatctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg
     4981 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     5041 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     5101 gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
     5161 gcaccatacc acagcttttc aattcatcat ttttttttta ttcttttttt tgatttcggt
     5221 ttccttgaaa tttttttgat tcggtaatct ccgaacagaa ggaagaacga aggaaggagc
     5281 acagacttag attggtatat atacgcatat gtagtgttga agaaacatga aattgcccag
     5341 tattcttaac ccaactgcac agaacaaaaa cctgcaggaa acgaagataa atcatgtcga
     5401 aagctacata taaggaacgt gctgctactc atcctagtcc tgttgctgcc aagctattta
     5461 atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt ggatgttcgt accaccaagg
     5521 aattactgga gttagttgaa gcattaggtc ccaaaatttg tttactaaaa acacatgtgg
     5581 atatcttgac tgatttttcc atggagggca cagttaagcc gctaaaggca ttatccgcca
     5641 agtacaattt tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat
     5701 tgcagtactc tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg
     5761 gtgtggtggg cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg
     5821 aacctagagg ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggag
     5881 aatatactaa gggtactgtt gacattgcga agagcgacaa agattttgtt atcggcttta
     5941 ttgctcaaag agacatgggt ggaagagatg aaggttacga ttggttgatt atgacacccg
     6001 gtgtgggttt agatgacaag ggagacgcat tgggtcaaca gtatagaacc gtggatgatg
     6061 tggtctctac aggatctgac attattattg ttggaagagg actatttgca aagggaaggg
     6121 atgctaaggt agagggtgaa cgttacagaa aagcaggctg ggaagcatat ttgagaagat
     6181 gtggccagca aaactaaaaa actgtattat aagtaaatgc atgtatacta aactcacaaa
     6241 ttagagcttc agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6301 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     6361 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     6421 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     6481 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6541 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6601 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6661 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6721 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6781 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6841 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6901 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6961 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7021 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7081 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7141 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7201 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7261 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7321 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     7381 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     7441 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     7501 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7561 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7621 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7681 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7741 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7801 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7861 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7921 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7981 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8041 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8101 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8161 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8221 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8281 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     8341 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     8401 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     8461 gagctccacc gcggtggcgg ccgctctaga actagtggat cccccgggct gcaggaattc
     8521 gatatcaagc ttatcgatac cgtcgacctc gagggggggc ccggtaccca attcgcccta
     8581 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     8641 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     8701 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     8761 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8821 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8881 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8941 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     9001 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     9061 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     9121 taagggattt tgccgatttc ggcctattgg t
//
