LOCUS       sequence-238768-        9963 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-LEU2 integrating (YIp) vector for stable genomic integration in
            the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9963)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9963)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-LEU2
FEATURES             Location/Qualifiers
     source          1..9963
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..152
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     386..403
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    520..541
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(556..561,562..579,580..586)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     599..621
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     618..634
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     618..634
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             630..1010
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     653..673
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        655..673
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    686..793
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     710..726
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     710..726
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(760..776)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(761..777)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(801..820)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(802..820)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(830..847)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(830..846)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(839..861)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      987..1442
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1074..1093)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1284..1305
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1499..1738
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1739..5917
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(6318..6337)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6437..6459
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6497..6515)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(6801..7895)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(7896..8303)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     promoter        8471..8575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8576..8644,8645..9436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8794..8813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9607..9963,1..232)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
       61 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
      121 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
      181 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
      241 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
      301 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
      361 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
      421 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
      481 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
      541 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
      601 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
      661 ccctcactaa agggaacaaa agctggagct ccaccgcggt ggcggccgct ctagaactag
      721 tggatccccc gggctgcagg aattcgatat caagcttatc gataccgtcg acctcgaggg
      781 ggggcccggt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
      841 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      901 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      961 agttgcgcag cctgaatggc gaatggacgc gccctgtagc ggcgcattaa gcgcggcggg
     1021 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     1081 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     1141 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     1201 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     1261 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     1321 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     1381 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     1441 ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catagatcaa
     1501 ttccacttgc aattacataa aaaattccgg cggtttttcg cgtgtgactc aatgtcgaaa
     1561 tacctgccta atgaacatga acatcgccca aatgtatttg aagacccgct gggagaagtt
     1621 caagatatat aagtaacaag cagccaatag tataaaaaaa aatctgagtt tattaccttt
     1681 cctggaattt cagtgaaaaa ctgctaatta tagagagata tcacagagtt actcactaat
     1741 gactaacgaa aaggtctgga tagagaagtt ggataatcca actctttcag tgttaccaca
     1801 tgacttttta cgcccacaac aagaacctta tacgaaacaa gctacatatt cgttacagct
     1861 acctcagctc gatgtgcctc atgatagttt ttctaacaaa tacgctgtcg ctttgagtgt
     1921 atgggctgca ttgatatata gagtaaccgg tgacgatgat attgttcttt atattgcgaa
     1981 taacaaaatc ttaagattca atattcaacc aacgtggtca tttaatgagc tgtattctac
     2041 aattaacaat gagttgaaca agctcaattc tattgaggcc aatttttcct ttgacgagct
     2101 agctgaaaaa attcaaagtt gccaagatct ggaaaggacc cctcagttgt tccgtttggc
     2161 ctttttggaa aaccaagatt tcaaattaga cgagttcaag catcatttag tggactttgc
     2221 tttgaatttg gataccagta ataatgcgca tgttttgaac ttaatttata acagcttact
     2281 gtattcgaat gaaagagtaa ccattgttgc ggaccaattt actcaatatt tgactgctgc
     2341 gctaagcgat ccatccaatt gcataactaa aatctctctg atcaccgcat catccaagga
     2401 tagtttacct gatccaacta agaacttggg ctggtgcgat ttcgtggggt gtattcacga
     2461 cattttccag gacaatgctg aagccttccc agagagaacc tgtgttgtgg agactccaac
     2521 actaaattcc gacaagtccc gttctttcac ttatcgcgac atcaaccgca cttctaacat
     2581 agttgcccat tatttgatta aaacaggtat caaaagaggt gatgtagtga tgatctattc
     2641 ttctaggggt gtggatttga tggtatgtgt gatgggtgtc ttgaaagccg gcgcaacctt
     2701 ttcagttatc gaccctgcat atcccccagc cagacaaacc atttacttag gtgttgctaa
     2761 accacgtggg ttgattgtta ttagagctgc tggacaattg gatcaactag tagaagatta
     2821 catcaatgat gaattggaga ttgtttcaag aatcaattcc atcgctattc aagaaaatgg
     2881 taccattgaa ggtggcaaat tggacaatgg cgaggatgtt ttggctccat atgatcacta
     2941 caaagacacc agaacaggtg ttgtagttgg accagattcc aacccaaccc tatctttcac
     3001 atctggttcc gaaggtattc ctaagggtgt tcttggtaga catttttcct tggcttatta
     3061 tttcaattgg atgtccaaaa ggttcaactt aacagaaaat gataaattca caatgctgag
     3121 cggtattgca catgatccaa ttcaaagaga tatgtttaca ccattatttt taggtgccca
     3181 attgtatgtc cctactcaag atgatattgg tacaccgggc cgtttagcgg aatggatgag
     3241 taagtatggt tgcacagtta cccatttaac acctgccatg ggtcaattac ttactgccca
     3301 agctactaca ccattcccta agttacatca tgcgttcttt gtgggtgaca ttttaacaaa
     3361 acgtgattgt ctgaggttac aaaccttggc agaaaattgc cgtattgtta atatgtacgg
     3421 taccactgaa acacagcgtg cagtttctta tttcgaagtt aaatcaaaaa atgacgatcc
     3481 aaactttttg aaaaaattga aagatgtcat gcctgctggt aaaggtatgt tgaacgttca
     3541 gctactagtt gttaacagga acgatcgtac tcaaatatgt ggtattggcg aaataggtga
     3601 gatttatgtt cgtgcaggtg gtttggccga aggttataga ggattaccag aattgaataa
     3661 agaaaaattt gtgaacaact ggtttgttga aaaagatcac tggaattatt tggataagga
     3721 taatggtgaa ccttggagac aattctggtt aggtccaaga gatagattgt acagaacggg
     3781 tgatttaggt cgttatctac caaacggtga ctgtgaatgt tgcggtaggg ctgatgatca
     3841 agttaaaatt cgtgggttca gaatcgaatt aggagaaata gatacgcaca tttcccaaca
     3901 tccattggta agagaaaaca ttactttagt tcgcaaaaat gccgacaatg agccaacatt
     3961 gatcacattt atggtcccaa gatttgacaa gccagatgac ttgtctaagt tccaaagtga
     4021 tgttccaaag gaggttgaaa ctgaccctat agttaagggc ttaatcggtt accatctttt
     4081 atccaaggac atcaggactt tcttaaagaa aagattggct agctatgcta tgccttcctt
     4141 gattgtggtt atggataaac taccattgaa tccaaatggt aaagttgata agcctaaact
     4201 tcaattccca actcccaagc aattaaattt ggtagctgaa aatacagttt ctgaaactga
     4261 cgactctcag tttaccaatg ttgagcgcga ggttagagac ttatggttaa gtatattacc
     4321 taccaagcca gcatctgtat caccagatga ttcgtttttc gatttaggtg gtcattctat
     4381 cttggctacc aaaatgattt ttaccttaaa gaaaaagctg caagttgatt taccattggg
     4441 cacaattttc aagtatccaa cgataaaggc ctttgccgcg gaaattgaca gaattaaatc
     4501 atcgggtgga tcatctcaag gtgaggtcgt cgaaaatgtc actgcaaatt atgcggaaga
     4561 cgccaagaaa ttggttgaga cgctaccaag ttcgtacccc tctcgagaat attttgttga
     4621 acctaatagt gccgaaggaa aaacaacaat taatgtgttt gttaccggtg tcacaggatt
     4681 tctgggctcc tacatccttg cagatttgtt aggacgttct ccaaagaact acagtttcaa
     4741 agtgtttgcc cacgtcaggg ccaaggatga agaagctgca tttgcaagat tacaaaaggc
     4801 aggtatcacc tatggtactt ggaacgaaaa atttgcctca aatattaaag ttgtattagg
     4861 cgatttatct aaaagccaat ttggtctttc agatgagaag tggatggatt tggcaaacac
     4921 agttgatata attatccata atggtgcgtt agttcactgg gtttatccat atgccaaatt
     4981 gagggatcca aatgttattt caactatcaa tgttatgagc ttagccgccg tcggcaagcc
     5041 aaagttcttt gactttgttt cctccacttc tactcttgac actgaatact actttaattt
     5101 gtcagataaa cttgttagcg aagggaagcc aggcatttta gaatcagacg atttaatgaa
     5161 ctctgcaagc gggctcactg gtggatatgg tcagtccaaa tgggctgctg agtacatcat
     5221 tagacgtgca ggtgaaaggg gcctacgtgg gtgtattgtc agaccaggtt acgtaacagg
     5281 tgcctctgcc aatggttctt caaacacaga tgatttctta ttgagatttt tgaaaggttc
     5341 agtccaatta ggtaagattc cagatatcga aaattccgtg aatatggttc cagtagatca
     5401 tgttgctcgt gttgttgttg ctacgtcttt gaatcctccc aaagaaaatg aattggccgt
     5461 tgctcaagta acgggtcacc caagaatatt attcaaagac tacttgtata ctttacacga
     5521 ttatggttac gatgtcgaaa tcgaaagcta ttctaaatgg aagaaatcat tggaggcgtc
     5581 tgttattgac aggaatgaag aaaatgcgtt gtatcctttg ctacacatgg tcttagacaa
     5641 cttacctgaa agtaccaaag ctccggaact agacgatagg aacgccgtgg catctttaaa
     5701 gaaagacacc gcatggacag gtgttgattg gtctaatgga ataggtgtta ctccagaaga
     5761 ggttggtata tatattgcat ttttaaacaa ggttggattt ttacctccac caactcataa
     5821 tgacaaactt ccactgccaa gtatagaact aactcaagcg caaataagtc tagttgcttc
     5881 aggtgctggt gctcgtggaa gctccgcagc agcttaaggt tgagcattac gtatgatatg
     5941 tccatgtaca ataattaaat atgaattagg agaaagactt agcttctttt cgggtgatgt
     6001 cacttaaaaa ctccgagaat aatatataat aagagaataa aatattagtt attgaataag
     6061 aactgtaaat cagctggcgt tagtctgcta atggcagctt catcttggtt tattgtagca
     6121 tgaatcatat ttgccttttt ttcctgtaat tcaatgattc ttgcttctat actatcctca
     6181 atgcaaaacc ttgtgatctt cacaggtcga tactgaccaa ttctatgaac tctatcacca
     6241 ctttgccatt caacactagg gttccaccat gggtctaaaa tgaatacttg cgaagctgat
     6301 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     6361 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     6421 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     6481 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgatcttt
     6541 ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag tgcaattctt tttccttatc
     6601 acgttgagcc attagtatca atttgcttac ctgtattcct ttacatcctc ctttttctcc
     6661 ttcttgataa atgtatgtag attgcgtata tagtttcgtc taccctatga acatattcca
     6721 ttttgtaatt tcgtgtcgtt tctattatga atttcattta taaagtttat gtacaaatat
     6781 cataaaaaaa gagaatcttt ttaagcaagg attttcttaa cttcttcggc gacagcatca
     6841 ccgacttcgg tggtactgtt ggaaccacct aaatcaccag ttctgatacc tgcatccaaa
     6901 acctttttaa ctgcatcttc aatggcctta ccttcttcag gcaagttcaa tgacaatttc
     6961 aacatcattg cagcagacaa gatagtggcg atagggttga ccttattctt tggcaaatct
     7021 ggagcagaac cgtggcatgg ttcgtacaaa ccaaatgcgg tgttcttgtc tggcaaagag
     7081 gccaaggacg cagatggcaa caaacccaag gaacctggga taacggaggc ttcatcggag
     7141 atgatatcac caaacatgtt gctggtgatt ataataccat ttaggtgggt tgggttctta
     7201 actaggatca tggcggcaga atcaatcaat tgatgttgaa ccttcaatgt agggaattcg
     7261 ttcttgatgg tttcctccac agtttttctc cataatcttg aagaggccaa aacattagct
     7321 ttatccaagg accaaatagg caatggtggc tcatgttgta gggccatgaa agcggccatt
     7381 cttgtgattc tttgcacttc tggaacggtg tattgttcac tatcccaagc gacaccatca
     7441 ccatcgtctt cctttctctt accaaagtaa atacctccca ctaattctct gacaacaacg
     7501 aagtcagtac ctttagcaaa ttgtggcttg attggagata agtctaaaag agagtcggat
     7561 gcaaagttac atggtcttaa gttggcgtac aattgaagtt ctttacggat ttttagtaaa
     7621 ccttgttcag gtctaacact accggtaccc catttaggac cacccacagc acctaacaaa
     7681 acggcatcag ccttcttgga ggcttccagc gcctcatctg gaagtggaac acctgtagca
     7741 tcgatagcag caccaccaat taaatgattt tcgaaatcga acttgacatt ggaacgaaca
     7801 tcagaaatag ctttaagaac cttaatggct tcggctgtga tttcttgacc aacgtggtca
     7861 cctggcaaaa cgacgatctt cttaggggca gacattagaa tggtatatcc ttgaaatata
     7921 tatatatata ttgctgaaat gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa
     7981 ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa gtattgtgat
     8041 gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc cggcgctctc
     8101 acctttcctt tttctcccaa tttttcagtt gaaaaaggta tatgcgtcag gcgacctctg
     8161 aaattaacaa aaaatttcca gtcatcgaat ttgattctgt gcgatagcgc ccctgtgtgt
     8221 tctcgttatg ttgaggaaaa aaataatggt tgctaagaga ttcgaactct tgcatcttac
     8281 gatacctgag tattcccaca gttaactgcg gtcaagatat ttcttgaatc aggcgcctta
     8341 gaccgctcgg ccaaacaacc aattacttgt tgagaaatag agtataatta tcctataaat
     8401 ataacgtttt tgaacacaca tgaacaagga agtacaggag acgtcaggtg gcacttttcg
     8461 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     8521 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     8581 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     8641 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     8701 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     8761 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     8821 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     8881 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     8941 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9001 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9061 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9121 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9181 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9241 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9301 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9361 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9421 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     9481 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     9541 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9601 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9661 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9721 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     9781 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9841 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9901 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9961 aac
//
