LOCUS       sequence-23878-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2w2-12-C81S
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             221..490
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             500..523
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cnaancgnac gcgcaatgca acctcccaat tcaaggtgcg gacaacaacc ctttgcaaca
       61 gcaatcatca cnantntacc ggtaccaaac gtcctcaacc aaattaacca aatcttttct
      121 ccagaggagc aacgcagctt attacaagaa gccatcgaaa cctgcaagaa ttttgaaaaa
      181 acacaattgt aagtctcggc tccaagaagg agatataccc atggcacgcg taactgttca
      241 ggacgctgta gagaaaattg gtaaccgttt tgacctggta ctggtcgccg cgcgtcgcgc
      301 tcgtcagatg caggtaggcg gaaaggaccc gctcgtaccg gaagaaaacg ataaaaccac
      361 tgtaatcgcg ctgcgcgaaa tcgaagaagg tctgatcaac aaccagatcc tcgacgttcg
      421 cgaacgccag gaacagcaag agcaggaagc cgctgaatta caagccgtta ccgctattgc
      481 tgaaggtcgt gcggccgcgg actacaagga tgacgacgac aagttccgga ccggctccaa
      541 gaccccgccg cacggcacgg cgcgcccata tgcttgccct gtcgagtcct gcgatcgccg
      601 cttttctcgc tcggatgagc ttacccgcca tatccgcatc cacacaggcc agaagccctt
      661 ccngtgtcga atctgcatgc gtaacttcag tcgtagtgac caccttacca cccacatccg
      721 cacccacacc ggttacggcc attgaaataa agataacagc tcacaggatt acgctttatt
      781 gacnttttgc aggattaggt gntttagact tgcttnagaa tcttgcggtc tgagtgcgtt
      841 atctaatga
//
