LOCUS       sequence-238781-        9011 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses E. coli codon optimized Cas12c2 effector in the pET28a 
            backbone..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9011)
  AUTHORS   Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, 
            Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng 
            DR, Scott DA
  TITLE     Functionally diverse type V CRISPR-Cas systems.
  JOURNAL   Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. 
            Epub 2018 Dec 6.
  PUBMED    30523077
REFERENCE   2  (bases 1 to 9011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aav7271"; journalName: "Science"; date: "2019-01-4-
            4"; volume: "363"; issue: "6422"; pages: "88-91"
COMMENT     Sequence Label: pET28a-mH6-Cas12c2
FEATURES             Location/Qualifiers
     source          1..9011
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..34
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(28..219)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(1028..2110)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2072..2091
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2111..2188)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     2394..2413
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     2497..2516
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2497..2515
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2516..2540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        join(2549..2554,2555..2572,2573..2578)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     RBS             2598..2620
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2652..2669
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6442..6460)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6456..6503
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6540..6995
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6627..6646)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6837..6858
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7087..7902)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7087..7902)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7810..7829
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7894..7913
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8024..8612
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8513..8532
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8766..8783
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8798..8937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8863..8882)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
       61 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
      121 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
      181 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
      241 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
      301 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
      361 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
      421 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
      481 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
      541 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
      601 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
      661 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
      721 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
      781 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
      841 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat
      901 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
      961 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
     1021 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     1081 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
     1141 ccagtgagac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
     1201 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
     1261 ggatataaca tgagctgtct tcggtatcgt cgtatcccac taccgagata tccgcaccaa
     1321 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
     1381 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
     1441 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
     1501 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
     1561 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtctt
     1621 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
     1681 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
     1741 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
     1801 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
     1861 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
     1921 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
     1981 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
     2041 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
     2101 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     2161 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     2221 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     2281 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     2341 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2401 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2461 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     2521 tgtgagcgga taacaattcc cctctagatt tacactttat gcttccggct cgtatgtttc
     2581 tagaaagctt aaataatttt gtttaacttt aagaaggaga tttaaatatg aaaatcgaag
     2641 aaggtaaagg tcaccatcac catcaccaca tgaccaaaca cagcatcccg ctgcatgcgt
     2701 tccgtaacag cggcgcggat gcgcgtaagt ggaaaggtcg tattgcgctg ctggcgaagc
     2761 gtggcaaaga aaccatgcgt accctgcagt ttccgctgga gatgagcgaa ccggaggcgg
     2821 cggcgatcaa caccaccccg tttgcggtgg cgtacaacgc gattgaaggc accggcaaag
     2881 gcaccctgtt cgactattgg gcgaagctgc acctggcggg ttttcgtttc tttccgagcg
     2941 gtggcgcggc gaccatcttc cgtcagcaag cggtttttga ggatgcgagc tggaacgcgg
     3001 cgttctgcca gcaaagcggc aaagactggc cgtggctggt gccgagcaag ctgtacgaac
     3061 gtttcaccaa agcgccgcgt gaggttgcga agaaagatgg tagcaagaaa agcatcgaat
     3121 ttacccagga gaacgtggcg aacgagagcc atgtgagcct ggttggcgcg agcattaccg
     3181 acaagacccc ggaagatcaa aaggagttct ttctgaaaat ggcgggtgcg ctggcggaaa
     3241 agttcgatag ctggaaaagc gcgaacgagg accgtatcgt ggcgatgaag gttattgacg
     3301 aatttctgaa aagcgagggt ctgcacctgc cgagcctgga aaacatcgcg gtgaagtgca
     3361 gcgttgaaac caaaccggat aacgcgaccg tggcgtggca tgatgcgccg atgagcggcg
     3421 tgcagaacct ggcgatcggt gttttcgcga cctgcgcgag ccgtatcgac aacatttacg
     3481 atctgaacgg tggcaagctg agcaaactga ttcaagaaag cgcgaccacc ccgaacgtta
     3541 ccgcgctgag ctggctgttc ggcaaaggtc tggagtattt tcgtaccacc gacatcgata
     3601 ccattatgca ggactttaac atcccggcga gcgcgaagga aagcattaaa ccgctggttg
     3661 agagcgcgca agcgatcccg accatgaccg ttctgggcaa gaaaaactac gcgccgttcc
     3721 gtccgaactt tggtggcaag atcgatagct ggattgcgaa ctatgcgagc cgtctgatgc
     3781 tgctgaacga catcctggaa cagattgagc cgggtttcga actgccgcaa gcgctgctgg
     3841 ataacgaaac cctgatgagc ggcatcgaca tgaccggtga tgaactgaaa gagctgattg
     3901 aagcggtgta cgcgtgggtt gatgcggcga aacaaggcct ggcgaccctg ctgggtcgtg
     3961 gtggcaacgt ggacgatgcg gttcagacct tcgaacaatt tagcgcgatg atggataccc
     4021 tgaacggcac cctgaacacc atcagcgcgc gttatgtgcg tgcggttgag atggcgggta
     4081 aagatgaagc gcgtctggag aagctgatcg aatgcaaatt cgacattccg aagtggtgca
     4141 aaagcgtgcc gaagctggtt ggtatcagcg gtggcctgcc gaaggtggag gaagagatta
     4201 aagttatgaa cgcggcgttt aaagacgtgc gtgcgcgtat gttcgttcgt tttgaagaga
     4261 ttgcggcgta tgtggcgagc aagggtgcgg gtatggacgt ttatgatgcg ctggaaaaac
     4321 gtgaactgga gcagattaag aaactgaaaa gcgcggtgcc ggagcgtgcg cacatccaag
     4381 cgtaccgtgc ggtgctgcat cgtattggtc gtgcggttca gaactgcagc gaaaagacca
     4441 aacaactgtt cagcagcaag gtgatcgaga tgggcgtttt taaaaacccg agccacctga
     4501 acaacttcat ctttaaccag aaaggtgcga tttaccgtag cccgttcgat cgtagccgtc
     4561 acgcgccgta tcaactgcat gcggacaagc tgctgaaaaa cgattggctg gagctgctgg
     4621 cggaaattag cgcgaccctg atggcgagcg agagcaccga acagatggag gacgcgctgc
     4681 gtctggaacg tacccgtctg cagctgcaac tgagcggcct gccggattgg gagtatccgg
     4741 cgagcctggc gaagccggac atcgaagttg agattcagac cgcgctgaag atgcaactgg
     4801 cgaaagacac cgtgaccagc gatgttctgc agcgtgcgtt caacctgtat agcagcgtgc
     4861 tgagcggtct gacctttaaa ctgctgcgtc gtagcttcag cctgaagatg cgttttagcg
     4921 ttgcggatac cacccaactg atctacgtgc cgaaggtttg cgactgggcg attccgaaac
     4981 agtatctgca agcggaaggc gagatcggta ttgcggcgcg tgtggttacc gaaagcagcc
     5041 cggcgaaaat ggtgaccgaa gttgagatga aggagccgaa agcgctgggc cacttcatgc
     5101 agcaagcgcc gcatgactgg tactttgatg cgagcctggg tggcacccag gtggcgggtc
     5161 gtatcgttga gaagggcaaa gaagtgggca aggagcgtaa actggttggc taccgtatgc
     5221 gtggtaacag cgcgtataag accgtgctgg acaaaagcct ggttggcaac accgaactga
     5281 gccagtgcag catgatcatt gagattccgt acacccaaac cgtggacgcg gatttccgtg
     5341 cgcaggttca agcgggtctg ccgaaggtga gcatcaacct gccggttaaa gaaaccatta
     5401 ccgcgagcaa caaggacgag cagatgctgt tcgatcgttt tgttgcgatt gacctgggtg
     5461 aacgtggcct gggttacgcg gtttttgatg cgaaaaccct ggaactgcaa gagagcggtc
     5521 accgtccgat caaggcgatt accaacctgc tgaaccgtac ccaccattat gagcagcgtc
     5581 cgaaccagcg tcaaaagttc caagcgaaat ttaacgttaa cctgagcgaa ctgcgtgaga
     5641 acaccgtggg tgacgtttgc catcagatca accgtatttg cgcgtactat aacgcgttcc
     5701 cggtgctgga atacatggtt ccggaccgtc tggataagca actgaaaagc gtgtacgaga
     5761 gcgttaccaa ccgttatatc tggagcagca ccgatgcgca caagagcgcg cgtgtgcagt
     5821 tttggctggg tggcgaaacc tgggagcatc cgtatctgaa gagcgcgaaa gacaagaaac
     5881 cgctggttct gagcccgggt cgtggtgcga gcggcaaagg caccagccaa acctgcagct
     5941 gctgcggccg taacccgttc gacctgatca aggatatgaa accgcgtgcg aagattgcgg
     6001 tggttgacgg caaggcgaaa ctggaaaaca gcgagctgaa actgtttgaa cgtaacctgg
     6061 agagcaagga cgatatgctg gcgcgtcgtc accgtaacga acgtgcgggt atggagcagc
     6121 cgctgacccc gggtaactac accgtggatg aaatcaaagc gctgctgcgt gcgaacctgc
     6181 gtcgtgcgcc gaagaaccgt cgtaccaaag acaccaccgt gagcgagtat cactgcgttt
     6241 tcagcgattg cggcaagacc atgcatgcgg acgaaaacgc ggcggttaac attggtggca
     6301 agtttatcgc ggacattgag aaataataaa agcttgaatt cctcgagaga gaccatggcc
     6361 accggtctct atcgaagata tgaggagctc aaactcgagc tgctaacaaa gcccgaaagg
     6421 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
     6481 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
     6541 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     6601 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     6661 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     6721 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     6781 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     6841 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     6901 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     6961 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     7021 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaat
     7081 taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca tatcaggatt
     7141 atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact caccgaggca
     7201 gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc caacatcaat
     7261 acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat caccatgagt
     7321 gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga cttgttcaac
     7381 aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt tattcattcg
     7441 tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat tacaaacagg
     7501 aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt cacctgaatc
     7561 aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca
     7621 tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa attccgtcag
     7681 ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt tgccatgttt
     7741 cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg cacctgattg
     7801 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
     7861 tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
     7921 gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt
     7981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8161 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     8821 cgcatctgtg cggtatttca caccgcaatg gtgcactctc agtacaatct gctctgatgc
     8881 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     8941 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     9001 tacagacaag c
//
