LOCUS       sequence-23879-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Noyes MB, Meng X, Wakabayashi A, Sinha S, Brodsky MH, Wolfe SA
  TITLE     A systematic characterization of factors that regulate Drosophila 
            segmentation via a bacterial one-hybrid system.
  JOURNAL   Nucleic Acids Res. 2008 May;36(8):2547-60. doi: 10.1093/nar/gkn048. 
            Epub 2008 Mar 10.
  PUBMED    18332042
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkn048"; journalName: "Nucleic Acids Res"; date: 
            "2008-05"; volume: "36"; issue: "8"; pages: "2547-60"
COMMENT     Sequence Label: pB1H2w5-12-C81S
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             219..488
                     /codon_start=1
                     /gene="E. coli rpoZ"
                     /product="DNA-directed RNA polymerase subunit omega"
                     /label=RNAP omega
                     /note="promotes RNA polymerase assembly"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDK
                     TTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGR"
     CDS             498..521
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cnanncntac gcagcaatgc aacctcccaa ttcaagtgcg gacaacaacc ctttgcaaca
       61 gcaatcatca caaaataccg taccaaacgt cctcaaccaa attaaccaaa tcttttctcc
      121 agaggagcaa cgcagcttat tacaagaagc catcgaaacc tgcaagaatt ttgaaaaaac
      181 acaattgtaa gtctcggctc caagaaggag atatacccat ggcacgcgta actgttcagg
      241 acgctgtaga gaaaattggt aaccgttttg acctggtact ggtcgccgcg cgtcgcgctc
      301 gtcagatgca ggtaggcgga aaggacccgc tcgtaccgga agaaaacgat aaaaccactg
      361 taatcgcgct gcgcgaaatc gaagaaggtc tgatcaacaa ccagatcctc gacgttcgcg
      421 aacgccagga acagcaagag caggaagccg ctgaattaca agccgttacc gctattgctg
      481 aaggtcgtgc ggccgcggac tacaaggatg acgacgacaa gttccggacc ggctccaaga
      541 ccccgccgca cggcacggcg cgcccatatg cttgccctgt cgagtcctgc gatcgccgct
      601 tttctcgctc ggatatncct gnnnnnntnc ccgcatccac acaggccaga agcccttcca
      661 gtgtcgaatc tgcatgcgta acttcagtcg tagtgaccac cttaccaccc acatccgcac
      721 ccacaccggt accgccattg aaataaaaga taaacagctc acaggattac gctttttgac
      781 tttttgcagg atnaggtgnt ttnnacntnt ntttaaantn ntgnggtgct gnggcgtttt
      841 tctatgatgg gataatntgc cca
//
