LOCUS       sequence-238808-        9111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-HIS3 integrating (YIp) vector for stable genomic integration in
            the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9111)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-HIS3
FEATURES             Location/Qualifiers
     source          1..9111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    89..110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(125..130,131..148,149..155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    163..179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     168..190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     187..203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     187..203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             199..579
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     222..242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        224..242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    255..362
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     279..295
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     279..295
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(329..345)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(330..346)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(370..389)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(371..389)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(399..416)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(399..415)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(408..430)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      556..1011
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(643..662)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     853..874
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1068..1307
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             1308..5486
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(5887..5906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6006..6028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6066..6084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             complement(6256..6918)
                     /codon_start=1
                     /gene="S. cerevisiae HIS3"
                     /product="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /label=HIS3
                     /note="yeast auxotrophic marker"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALGAVRGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHF
                     LESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(6919..7106)
                     /gene="S. cerevisiae HIS3"
                     /label=HIS3 promoter
     promoter        7188..7292
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7293..7361,7362..8153)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7511..7530)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8324..8912
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8813..8832
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9066..9083
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
       61 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
      121 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
      181 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
      241 gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg
      301 ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
      361 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
      421 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      481 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      541 ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
      601 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
      661 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
      721 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
      781 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
      841 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
      901 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
      961 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt tcctgatgcg
     1021 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatcaat tccacttgca
     1081 attacataaa aaattccggc ggtttttcgc gtgtgactca atgtcgaaat acctgcctaa
     1141 tgaacatgaa catcgcccaa atgtatttga agacccgctg ggagaagttc aagatatata
     1201 agtaacaagc agccaatagt ataaaaaaaa atctgagttt attacctttc ctggaatttc
     1261 agtgaaaaac tgctaattat agagagatat cacagagtta ctcactaatg actaacgaaa
     1321 aggtctggat agagaagttg gataatccaa ctctttcagt gttaccacat gactttttac
     1381 gcccacaaca agaaccttat acgaaacaag ctacatattc gttacagcta cctcagctcg
     1441 atgtgcctca tgatagtttt tctaacaaat acgctgtcgc tttgagtgta tgggctgcat
     1501 tgatatatag agtaaccggt gacgatgata ttgttcttta tattgcgaat aacaaaatct
     1561 taagattcaa tattcaacca acgtggtcat ttaatgagct gtattctaca attaacaatg
     1621 agttgaacaa gctcaattct attgaggcca atttttcctt tgacgagcta gctgaaaaaa
     1681 ttcaaagttg ccaagatctg gaaaggaccc ctcagttgtt ccgtttggcc tttttggaaa
     1741 accaagattt caaattagac gagttcaagc atcatttagt ggactttgct ttgaatttgg
     1801 ataccagtaa taatgcgcat gttttgaact taatttataa cagcttactg tattcgaatg
     1861 aaagagtaac cattgttgcg gaccaattta ctcaatattt gactgctgcg ctaagcgatc
     1921 catccaattg cataactaaa atctctctga tcaccgcatc atccaaggat agtttacctg
     1981 atccaactaa gaacttgggc tggtgcgatt tcgtggggtg tattcacgac attttccagg
     2041 acaatgctga agccttccca gagagaacct gtgttgtgga gactccaaca ctaaattccg
     2101 acaagtcccg ttctttcact tatcgcgaca tcaaccgcac ttctaacata gttgcccatt
     2161 atttgattaa aacaggtatc aaaagaggtg atgtagtgat gatctattct tctaggggtg
     2221 tggatttgat ggtatgtgtg atgggtgtct tgaaagccgg cgcaaccttt tcagttatcg
     2281 accctgcata tcccccagcc agacaaacca tttacttagg tgttgctaaa ccacgtgggt
     2341 tgattgttat tagagctgct ggacaattgg atcaactagt agaagattac atcaatgatg
     2401 aattggagat tgtttcaaga atcaattcca tcgctattca agaaaatggt accattgaag
     2461 gtggcaaatt ggacaatggc gaggatgttt tggctccata tgatcactac aaagacacca
     2521 gaacaggtgt tgtagttgga ccagattcca acccaaccct atctttcaca tctggttccg
     2581 aaggtattcc taagggtgtt cttggtagac atttttcctt ggcttattat ttcaattgga
     2641 tgtccaaaag gttcaactta acagaaaatg ataaattcac aatgctgagc ggtattgcac
     2701 atgatccaat tcaaagagat atgtttacac cattattttt aggtgcccaa ttgtatgtcc
     2761 ctactcaaga tgatattggt acaccgggcc gtttagcgga atggatgagt aagtatggtt
     2821 gcacagttac ccatttaaca cctgccatgg gtcaattact tactgcccaa gctactacac
     2881 cattccctaa gttacatcat gcgttctttg tgggtgacat tttaacaaaa cgtgattgtc
     2941 tgaggttaca aaccttggca gaaaattgcc gtattgttaa tatgtacggt accactgaaa
     3001 cacagcgtgc agtttcttat ttcgaagtta aatcaaaaaa tgacgatcca aactttttga
     3061 aaaaattgaa agatgtcatg cctgctggta aaggtatgtt gaacgttcag ctactagttg
     3121 ttaacaggaa cgatcgtact caaatatgtg gtattggcga aataggtgag atttatgttc
     3181 gtgcaggtgg tttggccgaa ggttatagag gattaccaga attgaataaa gaaaaatttg
     3241 tgaacaactg gtttgttgaa aaagatcact ggaattattt ggataaggat aatggtgaac
     3301 cttggagaca attctggtta ggtccaagag atagattgta cagaacgggt gatttaggtc
     3361 gttatctacc aaacggtgac tgtgaatgtt gcggtagggc tgatgatcaa gttaaaattc
     3421 gtgggttcag aatcgaatta ggagaaatag atacgcacat ttcccaacat ccattggtaa
     3481 gagaaaacat tactttagtt cgcaaaaatg ccgacaatga gccaacattg atcacattta
     3541 tggtcccaag atttgacaag ccagatgact tgtctaagtt ccaaagtgat gttccaaagg
     3601 aggttgaaac tgaccctata gttaagggct taatcggtta ccatctttta tccaaggaca
     3661 tcaggacttt cttaaagaaa agattggcta gctatgctat gccttccttg attgtggtta
     3721 tggataaact accattgaat ccaaatggta aagttgataa gcctaaactt caattcccaa
     3781 ctcccaagca attaaatttg gtagctgaaa atacagtttc tgaaactgac gactctcagt
     3841 ttaccaatgt tgagcgcgag gttagagact tatggttaag tatattacct accaagccag
     3901 catctgtatc accagatgat tcgtttttcg atttaggtgg tcattctatc ttggctacca
     3961 aaatgatttt taccttaaag aaaaagctgc aagttgattt accattgggc acaattttca
     4021 agtatccaac gataaaggcc tttgccgcgg aaattgacag aattaaatca tcgggtggat
     4081 catctcaagg tgaggtcgtc gaaaatgtca ctgcaaatta tgcggaagac gccaagaaat
     4141 tggttgagac gctaccaagt tcgtacccct ctcgagaata ttttgttgaa cctaatagtg
     4201 ccgaaggaaa aacaacaatt aatgtgtttg ttaccggtgt cacaggattt ctgggctcct
     4261 acatccttgc agatttgtta ggacgttctc caaagaacta cagtttcaaa gtgtttgccc
     4321 acgtcagggc caaggatgaa gaagctgcat ttgcaagatt acaaaaggca ggtatcacct
     4381 atggtacttg gaacgaaaaa tttgcctcaa atattaaagt tgtattaggc gatttatcta
     4441 aaagccaatt tggtctttca gatgagaagt ggatggattt ggcaaacaca gttgatataa
     4501 ttatccataa tggtgcgtta gttcactggg tttatccata tgccaaattg agggatccaa
     4561 atgttatttc aactatcaat gttatgagct tagccgccgt cggcaagcca aagttctttg
     4621 actttgtttc ctccacttct actcttgaca ctgaatacta ctttaatttg tcagataaac
     4681 ttgttagcga agggaagcca ggcattttag aatcagacga tttaatgaac tctgcaagcg
     4741 ggctcactgg tggatatggt cagtccaaat gggctgctga gtacatcatt agacgtgcag
     4801 gtgaaagggg cctacgtggg tgtattgtca gaccaggtta cgtaacaggt gcctctgcca
     4861 atggttcttc aaacacagat gatttcttat tgagattttt gaaaggttca gtccaattag
     4921 gtaagattcc agatatcgaa aattccgtga atatggttcc agtagatcat gttgctcgtg
     4981 ttgttgttgc tacgtctttg aatcctccca aagaaaatga attggccgtt gctcaagtaa
     5041 cgggtcaccc aagaatatta ttcaaagact acttgtatac tttacacgat tatggttacg
     5101 atgtcgaaat cgaaagctat tctaaatgga agaaatcatt ggaggcgtct gttattgaca
     5161 ggaatgaaga aaatgcgttg tatcctttgc tacacatggt cttagacaac ttacctgaaa
     5221 gtaccaaagc tccggaacta gacgatagga acgccgtggc atctttaaag aaagacaccg
     5281 catggacagg tgttgattgg tctaatggaa taggtgttac tccagaagag gttggtatat
     5341 atattgcatt tttaaacaag gttggatttt tacctccacc aactcataat gacaaacttc
     5401 cactgccaag tatagaacta actcaagcgc aaataagtct agttgcttca ggtgctggtg
     5461 ctcgtggaag ctccgcagca gcttaaggtt gagcattacg tatgatatgt ccatgtacaa
     5521 taattaaata tgaattagga gaaagactta gcttcttttc gggtgatgtc acttaaaaac
     5581 tccgagaata atatataata agagaataaa atattagtta ttgaataaga actgtaaatc
     5641 agctggcgtt agtctgctaa tggcagcttc atcttggttt attgtagcat gaatcatatt
     5701 tgcctttttt tcctgtaatt caatgattct tgcttctata ctatcctcaa tgcaaaacct
     5761 tgtgatcttc acaggtcgat actgaccaat tctatgaact ctatcaccac tttgccattc
     5821 aacactaggg ttccaccatg ggtctaaaat gaatacttgc gaagctgatc tatggtgcac
     5881 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     5941 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     6001 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     6061 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgatgcctc gttcagaatg
     6121 acacgtatag aatgatgcat taccttgtca tcttcagtat catactgttc gtatacatac
     6181 ttactgacat tcataggtat acatatatac acatgtatat atatcgtatg ctgcagcttt
     6241 aaataatcgg tgtcactaca taagaacacc tttggtggag ggaacatcgt tggtaccatt
     6301 gggcgaggtg gcttctctta tggcaaccgc aagagccttg aacgcactct cactacggtg
     6361 atgatcattc ttgcctcgca gacaatcaac gtggagggta attctgctag cctctgcaaa
     6421 gctttcaaga aaatgcggga tcatctcgca agagagatct cctactttct ccctttgcaa
     6481 accaagttcg acaactgcgt acggcctgtt cgaaagatct accaccgctc tggaaagtgc
     6541 ctcatccaaa ggcgcaaatc ctgatccaaa cctttttact ccacgcacgg cccctagggc
     6601 ctctttaaaa gcttgaccga gagcaatccc gcagtcttca gtggtgtgat ggtcgtctat
     6661 gtgtaagtca ccaatgcact caacgattag cgaccagccg gaatgcttgg ccagagcatg
     6721 tatcatatgg tccagaaacc ctatacctgt gtggacgtta atcacttgcg attgtgtggc
     6781 ctgttctgct actgcttctg cctctttttc tgggaagatc gagtgctcta tcgctagggg
     6841 accacccttt aaagagatcg caatctgaat cttggtttca tttgtaatac gctttactag
     6901 ggctttctgc tctgtcatct ttgccttcgt ttatcttgcc tgctcatttt ttagtatatt
     6961 cttcgaagaa atcacattac tttatataat gtataattca ttatgtgata atgccaatcg
     7021 ctaagaaaaa aaaagagtca tccgctaggt ggaaaaaaaa aaatgaaaat cattaccgag
     7081 gcataaaaaa atatagagtg tactagagga ggccaagagt aatagaaaaa gaaaattgcg
     7141 ggaaaggact gtgttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7201 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7261 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7321 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7381 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7441 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7501 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7561 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7621 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7681 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7741 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7801 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7861 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7921 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7981 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8041 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8101 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8161 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8221 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8281 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8341 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8401 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8461 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8521 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8581 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8641 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8701 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8761 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8821 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8881 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8941 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     9001 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     9061 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa c
//
