LOCUS       sequence-238823-        9686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses translation elongation-controlled Renilla luciferase and 
            translation initiation-controlled firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9686)
  TITLE     Elongation controlled transcripts in yeast
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTH825-CEN-minRLuc/slowstaCFLuc
FEATURES             Location/Qualifiers
     source          1..9686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     247..264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    381..402
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(417..422,423..440,441..447)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    455..471
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     460..482
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     479..495
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     479..495
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        516..534
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     terminator      complement(582..881)
                     /label=ADH2 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 2 (ADH2) gene"
     promoter        complement(1852..2248)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     promoter        2257..2900
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     2793..2816
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(3480..3498)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     terminator      5213..5400
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(5268..5292)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(5465..5484)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5466..5484)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5491..5508)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5491..5507)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5500..5522)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5648..6103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5735..5754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5945..5966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6234..7037)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(7038..7253)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(7277..7296)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7456..7474)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    7515..8018
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        8055..8159
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8160..8228,8229..9020)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8378..8397)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9191..9686,1..93)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     join(9680..9686,1..13)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
       61 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      121 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
      181 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
      241 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
      301 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
      361 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
      421 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
      481 ggaaacagct atgaccatga ttacgccaag ctcgaaatta accctcacta aagggaacaa
      541 aagctggtac cgggcccccc ctcgagggca tgcgaaggaa aatgagaaat atcgagggag
      601 acgattcaga ggagcaggac aaactataac cgactgtttg ttggaggatg ccgtacataa
      661 cgaacactgc tgaagctacc atgtctacag tttagaggaa tgggtacaac tcacaggcga
      721 gggatggtgt tcactcgtgc tagcaaacgc ggtgggagca aaaagtagaa tattatcttt
      781 tattcgtgaa acttcgaaca ctgtcatcta aagatgctat atactaatat aggcatactt
      841 gataatgaaa actataaatc gtaaagacat aagagatccg cttatttaga agtgtcgaat
      901 tcttacgaca tctgctcatt cttgagtacc ctctctacga acgactttat atacttcccc
      961 atctcatcag gtgcatcctc ctgcgagaag tggagcccct ttacctttac gaactctgta
     1021 ttagggaact tctttgcccc ctctactatt gcattcgaga agaacccagg atcggactct
     1081 atgaacatct tagggagatc atccgatgcc ctgagatatg cattataatt ccttactatc
     1141 tgtactacat caggcttccc cccctttacg agaggtatct ccctaggcca cgagagtgta
     1201 ggcctcctta cctccccctt ctccttgaaa ggctcgagat atgctgcgaa ctcctcaggc
     1261 tcgagcttcc tcattatctt cgaagggagc attgtctcta cgaagaaatt attctcgagt
     1321 accatcttct ccccctcctc cgactttatg agtgctatat cctcctctat atcaggccac
     1381 tcatcccacg actctattac atctactacc gactctgcgt gtactattgc ctttatctta
     1441 tcctggtgct catacgaata gtggaatgcg aggcatgccc cccaatcgtg ccctacgaat
     1501 attatcttct tagggagatt gaggagctcg aaccatgctg tgagatactt atagtgatcg
     1561 aggagcctat acgacccatt ccccgacttc cccgacttcc ccatccctat gagatcaggt
     1621 attatgcacc ttgctacagg ctctatgtga ggtactacgt gcctccagag atacgacgat
     1681 gctgcattcc cgtggaggaa tattactgca ttctctgcgt gcttctccga atcataataa
     1741 tttatgaacg aatcgagtac attcatctgc ttgcaccttg cccaccactg aggccctgtt
     1801 atcatcctct tcctctgctc aggatcatat accttcgatg tcatcccggg gagttgattg
     1861 tatgcttggt atagcttgaa atattgtgca gaaaaagaaa caaggaagaa agggaacgag
     1921 aacaatgacg aggaaacaaa agattaataa ttgcaggtct atttatactt gatagcaaga
     1981 cagcaaactt ttttttattt caaattcaag taactggaag gaaggccgta taccgttgct
     2041 cattagagag tagtgtgcgt gaatgaagga aggaaaaagt ttcgtgtgct tcgagatacc
     2101 cctcatcagc tctggaacaa cgacatctgt tggtgctgtc tttgtcgtta attttttcct
     2161 ttagtgtctt ccatcatttt tttgtcattg cggatatggt gagacaacaa cgggggagag
     2221 agaaaagaaa aaaaaagaaa agaatccggg agtttatcat tatcaatact gccatttcaa
     2281 agaatacgta aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc
     2341 cttttaattc tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata
     2401 taacatcgta ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc
     2461 ccgcttttta agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct
     2521 tcaccaacca tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca
     2581 aacaggcaaa aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat
     2641 gacacaaggc aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct
     2701 tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt
     2761 attcccctac ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa
     2821 tctatttctt aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa
     2881 caccaagaac ttagtttcga cggatctagt ttcactagcg aattatacaa ctcaccagcc
     2941 acacagctca ctcatctact tcgcaatcaa aacaaaatat tttattttag ttcagtttat
     3001 taagttatta tcagtatcgt attaaaaaat taaagatcat tgaaaaatgg cttgctaaac
     3061 cgattatatt ttgtttttaa agtagattat tattagaaaa ttattaagag aattctgtgt
     3121 taaatttatt gaaagagaaa atttattttc ccttattaat taaagtcctt tacttttttt
     3181 gaaaactgtc agttttttga agagttattt gttttgttac caattgctat caggtacccg
     3241 tagaatttta ttcaagatct ttccgtaacg gttacctttc tgtcaaatta tccaggttta
     3301 ctcgccaata aaaatttccc tatactatca ttaattaaat cattattatt actaaagttt
     3361 tgtttaccaa tttgtctgct caagaaaata aattaaatac aaataaagga tccatggaag
     3421 acgccaaaaa cataaagaaa ggcccggcgc cattctatcc tctagaggat ggaaccgctg
     3481 gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca attgctttta
     3541 cagatgcaca tatcgaggtg aacatcacgt acgcggaata cttcgaaatg tccgttcggt
     3601 tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc gtatgcagtg
     3661 aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga gttgcagttg
     3721 cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgaac atttcgcagc
     3781 ctaccgtagt gtttgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg caaaaaaaat
     3841 taccaataat ccagaaaatt attatcatgg attctaaaac ggattaccag ggatttcagt
     3901 cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac gattttgtac
     3961 cagagtcctt tgatcgtgac aaaacaattg cactgataat gaattcctct ggatctactg
     4021 ggttacctaa gggtgtggcc cttccgcata gaactgcctg cgtcagattc tcgcatgcca
     4081 gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt gttgttccat
     4141 tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga tttcgagtcg
     4201 tcttaatgta tagatttgaa gaagagctgt ttttacgatc ccttcaggat tacaaaattc
     4261 aaagtgcgtt gctagtacca accctatttt cattcttcgc caaaagcact ctgattgaca
     4321 aatacgattt atctaattta cacgaaattg cttctggggg cgcacctctt tcgaaagaag
     4381 tcggggaagc ggttgcaaaa cgcttccatc ttccagggat acgacaagga tatgggctca
     4441 ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg ggcgcggtcg
     4501 gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg aaaacgctgg
     4561 gcgttaatca gagaggcgaa ttatgtgtca gaggacctat gattatgtcc ggttatgtaa
     4621 acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat tctggagaca
     4681 tagcttactg ggacgaagac gaacacttct tcatagttga ccgcttgaag tctttaatta
     4741 aatacaaagg atatcaggtg gcccccgctg aattggaatc gatattgtta caacacccca
     4801 acatcttcga cgcgggcgtg gcaggtcttc ccgacgatga cgccggtgaa cttcccgccg
     4861 ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg gattacgtcg
     4921 ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg gacgaagtac
     4981 cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc ataaaggcca
     5041 agaagggcgg aaagtaagtc gacctgcagg catgcaagct ttggacttct tcgccagagg
     5101 tttggtcaag tctccaatca aggttgtcgg cttgtctacc ttgccagaaa tttacgaaaa
     5161 gatggaaaag ggtcaaatcg ttggtagata cgttgttgac acttctaaat aagcgaattt
     5221 cttatgattt atgattttta ttattaaata agttataaaa aaaataagtg tatacaaatt
     5281 ttaaagtgac tcttaggttt taaaacgaaa attcttattc ttgagtaact ctttcctgta
     5341 ggtcaggttg ctttctcagg tatagcatga ggtcgctctt attgaccaca cctctaccgg
     5401 catgccggtc gaaattcccc taccctatga aaagcggccg ccaccgcggt ggagctccaa
     5461 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg
     5521 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     5581 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     5641 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     5701 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     5761 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     5821 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     5881 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     5941 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     6001 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     6061 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttcctgatg cggtattttc
     6121 tccttacgca tctgtgcggt atttcacacc gcatagggta ataactgata taattaaatt
     6181 gaagctctaa tttgtgagtt tagtatacat gcatttactt ataatacagt tttttagttt
     6241 tgctggccgc atcttctcaa atatgcttcc cagcctgctt ttctgtaacg ttcaccctct
     6301 accttagcat cccttccctt tgcaaatagt cctcttccaa caataataat gtcagatcct
     6361 gtagagacca catcatccac ggttctatac tgttgaccca atgcgtctcc cttgtcatct
     6421 aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt catctcttcc acccatgtct
     6481 ctttgagcaa taaagccgat aacaaaatct ttgtcgctct tcgcaatgtc aacagtaccc
     6541 ttagtatatt ctccagtaga tagggagccc ttgcatgaca attctgctaa catcaaaagg
     6601 cctctaggtt cctttgttac ttcttctgcc gcctgcttca aaccgctaac aatacctggg
     6661 cccaccacac cgtgtgcatt cgtaatgtct gcccattctg ctattctgta tacacccgca
     6721 gagtactgca atttgactgt attaccaatg tcagcaaatt ttctgtcttc gaagagtaaa
     6781 aaattgtact tggcggataa tgcctttagc ggcttaactg tgccctccat ggaaaaatca
     6841 gtcaagatat ccacatgtgt ttttagtaaa caaattttgg gacctaatgc ttcaactaac
     6901 tccagtaatt ccttggtggt acgaacatcc aatgaagcac acaagtttgt ttgcttttcg
     6961 tgcatgatat taaatagctt ggcagcaaca ggactaggat gagtagcagc acgttcctta
     7021 tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg tttttgttct gtgcagttgg
     7081 gttaagaata ctgggcaatt tcatgtttct tcaacactac atatgcgtat atataccaat
     7141 ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg agattaccga atcaaaaaaa
     7201 tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa atgatgaatt gaaaaggtgg
     7261 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     7321 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7381 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7441 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     7501 tggtttctta gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg
     7561 gaataatttg ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag
     7621 aaagtaaata aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt
     7681 aaaaaatttc aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt
     7741 aaatagatat acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt
     7801 ggtcttctac acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca
     7861 agataaaagg tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa
     7921 aaactatttt ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt
     7981 aaaaaattta aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt
     8041 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     8101 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     8161 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     8221 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     8281 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     8341 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     8401 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     8461 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     8521 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     8581 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     8641 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     8701 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     8761 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     8821 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     8881 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     8941 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     9001 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     9061 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     9121 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     9181 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     9241 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     9301 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     9361 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     9421 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     9481 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     9541 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     9601 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     9661 gcacgaggga gcttccaggg ggaaac
//
