LOCUS       sequence-238827-        9888 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector (pLenti6.3/TO/DEST) expresses EYFP, P2A, and 
            IDH1 C-terminally tagged with HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9888)
  AUTHORS   Tiburcio PDB, Xiao B, Berg S, Asper S, Lyne S, Zhang Y, Zhu X, Yan 
            H, Huang LE
  TITLE     Functional requirement of a wild-type allele for mutant IDH1 to 
            suppress anchorage-independent growth through redox homeostasis.
  JOURNAL   Acta Neuropathol. 2018 Feb;135(2):285-298. doi: 
            10.1007/s00401-017-1800-0. Epub 2017 Dec 29.
  PUBMED    29288440
REFERENCE   2  (bases 1 to 9888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00401-017-1800-0"; journalName: "Acta Neuropathol"; date: 
            "2018-02"; volume: "135"; issue: "2"; pages: "285-298"
COMMENT     Sequence Label: pLenti/TO/EYFP-P2A-IDH1(HA)
FEATURES             Location/Qualifiers
     source          1..9888
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    128..149
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(164..169,170..187,188..194)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    202..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     207..229
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     226..242
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     226..242
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     261..281
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        263..281
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        309..535
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             536..716
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    763..888
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1381..1614
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1799..1843
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2110..2227
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2553..2756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2707..2727
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2758..2776
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2779..2797
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2838..2862
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2868..2870,2871..2873,2874..3584)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2913..2934)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3174..3193)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3521..3542
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3609..3629
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3633..3653
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3657..3677
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3705..3761
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5001..5027
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5031..5055)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             5108..5173
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    5197..5785
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5250..5270)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5668..5679)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5800..6116
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5967..6102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6164..6211
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6230..6628
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             6715..6948
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7020..7141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7057..7076)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7111..7130
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7181..7316
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7336..7355)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7337..7355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7365..7382)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7365..7381)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7374..7396)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7523..7978
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7610..7629)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7820..7841
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8004..8108
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8109..8177,8178..8969)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8327..8346)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9140..9728
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9629..9648
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(9882..9888,1..11)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
       61 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      121 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      181 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      241 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
      301 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
      361 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      421 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      481 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      541 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      601 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      661 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      721 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      781 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      841 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      901 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      961 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     1021 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     1081 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     1141 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     1201 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     1261 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1321 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1381 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1441 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1501 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1561 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1621 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1681 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1741 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1801 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1861 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1921 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1981 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2041 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2101 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     2161 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     2221 aaattttatc gataagcttg ggagttccgc ttacataact tacggtaaat ggcccgcctg
     2281 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2341 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2401 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2461 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2521 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2581 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2641 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2701 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctctcc
     2761 ctatcagtga tagagatctc cctatcagtg atagagatcg tcgactagtc cagtgtggtg
     2821 gaattctgca gatatcaaca agtttgtaca aaaaagcagg cttcaccatg gtgagcaagg
     2881 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     2941 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3001 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct
     3061 tcggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     3121 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3181 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3241 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3301 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3361 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3421 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     3481 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3541 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtccgga ctcagatctc
     3601 gagctgatcc aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa
     3661 aaaagaagag aaaggtagga tccaccggat ctagaggaag cggagctact aacttcagcc
     3721 tgctgaagca ggctggagac gtggaggaga accctggacc ttccaaaaaa atcagtggcg
     3781 gttctgtggt agagatgcaa ggagatgaaa tgacacgaat catttgggaa ttgattaaag
     3841 agaaactcat ttttccctac gtggaattgg atctacatag ctatgattta ggcatagaga
     3901 atcgtgatgc caccaacgac caagtcacca aggatgctgc agaagctata aagaagcata
     3961 atgttggcgt caaatgtgcc actatcactc ctgatgagaa gagggttgag gagttcaagt
     4021 tgaaacaaat gtggaaatca ccaaatggca ccatacgaaa tattctgggt ggcacggtct
     4081 tcagagaagc cattatctgc aaaaatatcc cccggcttgt gagtggatgg gtaaaaccta
     4141 tcatcatagg tcgtcatgct tatggggatc aatacagagc aactgatttt gttgttcctg
     4201 ggcctggaaa agtagagata acctacacac caagtgacgg aacccaaaag gtgacatacc
     4261 tggtacataa ctttgaagaa ggtggtggtg ttgccatggg gatgtataat caagataagt
     4321 caattgaaga ttttgcacac agttccttcc aaatggctct gtctaagggt tggcctttgt
     4381 atctgagcac caaaaacact attctgaaga aatatgatgg gcgttttaaa gacatctttc
     4441 aggagatata tgacaagcag tacaagtccc agtttgaagc tcaaaagatc tggtatgagc
     4501 ataggctcat cgacgacatg gtggcccaag ctatgaaatc agagggaggc ttcatctggg
     4561 cctgtaaaaa ctatgatggt gacgtgcagt cggactctgt ggcccaaggg tatggctctc
     4621 tcggcatgat gaccagcgtg ctggtttgtc cagatggcaa gacagtagaa gcagaggctg
     4681 cccacgggac tgtaacccgt cactaccgca tgtaccagaa aggacaggag acgtccacca
     4741 atcccattgc ttccattttt gcctggacca gagggttagc ccacagagca aagcttgata
     4801 acaataaaga gcttgccttc tttgcaaatg ctttggaaga agtctctatt gagacaattg
     4861 aggctggctt catgaccaag gacttggctg cttgcattaa aggtttaccc aatgtgcaac
     4921 gttctgacta cttgaataca tttgagttca tggataaact tggagaaaac ttgaagatca
     4981 aactagctca ggccaaactt tacccatacg acgtgcctga ctacgcctag acccagcttt
     5041 cttgtacaaa gtggttgata tccagcacag tggcggccgc tcgagtctag agggcccgcg
     5101 gttcgaagac tacaaagacc atgacggtga ttataaagat catgacatcg attacaagga
     5161 tgacgatgac aagcgtaccg gttagtaatg atcgacaatc aacctctgga ttacaaaatt
     5221 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     5281 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     5341 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     5401 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     5461 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     5521 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     5581 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     5641 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     5701 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     5761 atctcccttt gggccgcctc cccgcctggc gatggtaccg gtgtggaaag tccccaggct
     5821 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     5881 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     5941 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     6001 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     6061 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     6121 tcccgggagc ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc
     6181 ggcatagtat atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc
     6241 tttgtctcaa gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc
     6301 catctctgaa gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac
     6361 tggtgtcaat gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac
     6421 tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag
     6481 gggcatcttg agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat
     6541 caaagccata gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct
     6601 gccctctggt tatgtgtggg agggctaagc acaattcgag ctcggtacct ttaagaccaa
     6661 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     6721 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     6781 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     6841 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     6901 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
     6961 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     7021 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     7081 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     7141 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     7201 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     7261 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     7321 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     7381 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     7441 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     7501 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7561 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7621 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7681 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7741 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7801 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7861 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7921 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     7981 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8041 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     8101 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     8161 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     8221 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     8281 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     8341 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     8401 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     8461 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     8521 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     8581 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     8641 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     8701 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     8761 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     8821 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     8881 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     8941 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     9001 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     9061 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     9121 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     9181 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     9241 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     9301 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     9361 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     9421 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     9481 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     9541 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     9601 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     9661 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     9721 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     9781 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     9841 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagt
//
