LOCUS       sequence-238852-       12593 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses HA-tagged human NPHP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12593)
  AUTHORS   Takemaru et al.
  TITLE     Takemaru Lab Unpublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-EF1alpha-IRES-EGFP-HA-hNPHP1
FEATURES             Location/Qualifiers
     source          1..12593
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1209..1235
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(3115..3689,3690..3692,3693..3701)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3282..3299)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3509..3528
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3702..3704,3705..3707,3708..4421)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3702..3704,3705..3707,3708..4418)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3747..3768)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4008..4027)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4355..4376
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    4431..5019
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4484..4504)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4902..4913)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5543..5723
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5749..5766)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5755..5979
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6025..6453
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6112..6131)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6322..6343
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6462..6482)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6467..6796
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6647..6782
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6709..6728
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6844..6891
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6910..7284
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7414..7535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7451..7470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7505..7524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7584..7600)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7584..7600)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7597..7619)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7608..7624
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7632..7638,7639..7656,7657..7662))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7677..7698
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8238..8826)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8318..8337)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8997..9788,9789..9857))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9620..9639
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9858..9962)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10037..10056)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10228..10607
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10609..10807
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             10825..11005
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11052..11177
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11670..11903
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12088..12132
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12430..12547
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgtgg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattcggcc attacggcca
     1201 ccatgcgata cccatatgat gtgccagatt acgctggatc catgctggcg agacgacagc
     1261 gagatcctct ccaggccctg cggcgccgca atcaggagct gaagcaacag gttgatagtt
     1321 tgctttctga gagccaactg aaagaagctc tagaacccaa taaaagacaa catatttatc
     1381 aaagagttgg ggcacctact gaagaagagg aagaaagtga aagtgaagat agtgaagaca
     1441 gtggtgggga ggaagaagat gcagaggagg aagaggaaga gaaagaggaa aatgaatctc
     1501 acaaatggtc aaccggtgaa gaatacatcg ctgttggaga ttttactgct cagcaagttg
     1561 gagatcttac atttaagaaa ggggaaattc tccttgtaat tgaaaaaaaa cctgatggtt
     1621 ggtggatagc taaggatgcc aaaggaaatg aaggtcttgt tcccagaacc tacctagagc
     1681 cttatagtga agaagaagaa ggccaagagt caagtgaaga gggcagtgaa gaagatgtag
     1741 aggcggtgga tgaaacagca gatggagcag aagttaagca aagaactgat ccccactgga
     1801 gtgctgttca gaaagcgatt tcagagataa acactgttga tgtgttaact acgatgggag
     1861 ctattcctgc agggttcagg ccttccacgc tctcacagct tctggaggaa gggaatcaat
     1921 ttcgagcaaa ttacttctta caaccagagc tcatgccttc acaactggcc ttcagagatc
     1981 tgatgtggga tgctacagaa ggcactatta ggtcgagacc aagtcgtatt tcattgattc
     2041 tgacattatg gagctgtaaa atgattcctc ttccaggaat gagcatacag gttctcagca
     2101 gacatgtacg cctctgtcta tttgatggta ataaggttct gagcaacatt catacagtca
     2161 gagccacatg gcaacctaaa aagcccaaaa catggacctt ttctccccag gttactcgca
     2221 tcttaccatg tttgcttgat ggtgattgct ttatcaggtc taattctgca tctccagatc
     2281 ttggaatatt atttgaactt ggaatttctt atattcgcaa ttcaactggt gaaagaggag
     2341 agttaagctg tggctgggtg tttcttaaac tttttgatgc cagtggagtt cctattccag
     2401 caaaaactta tgagcttttc ttgaatggtg gtactcctta tgaaaaaggt attgaagtgg
     2461 acccttcaat atccagaaga gcacacggca gtgttttcta ccagattatg acaatgagaa
     2521 ggcagcctca acttctagtg aaactgagat ccttgaacag aagatcaaga aatgtactaa
     2581 gtctactgcc agaaacatta attggaaata tgtgttctat tcacttgttg atattttatc
     2641 gacaaattct tggagatgtg ctcctgaaag acaggatgag cttgcaaagt actgatttaa
     2701 ttagccatcc catgctggcc accttcccca tgctcttgga gcagcctgat gtgatggatg
     2761 ctctcaggag ttcgtgggct ggaaaagaaa gcacattaaa aagatcagag aagagagaca
     2821 aagagttcct gaagtccacg tttctcctgg tttaccatga ctgcgtgctc ccacttctcc
     2881 actccacacg cctaccccca ttcaggtggg cagaagaaga gactgagact gcacggtgga
     2941 aagttatcac tgacttcctt aagcaaaacc aagaaaacca gggcgccctc caagctctgc
     3001 tgtcaccaga cggagttcat gaaccttttg acctttcaga gcagacctat gacttcttgg
     3061 gtgaaatgag aaagaatgca gtgtgaggcc gcctcggcca tcgataagga tccgcccctc
     3121 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     3181 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     3241 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     3301 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     3361 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     3421 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     3481 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     3541 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
     3601 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     3661 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     3721 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3781 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     3841 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     3901 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3961 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     4021 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     4081 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     4141 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     4201 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     4261 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     4321 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     4381 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc aatcaacctc
     4441 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4501 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4561 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4621 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     4681 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     4741 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     4801 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     4861 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     4921 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     4981 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgcctgcagg tttgtcgaga
     5041 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     5101 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     5161 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5221 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     5281 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     5341 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     5401 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     5461 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     5521 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     5581 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     5641 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5701 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     5761 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     5821 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     5881 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     5941 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     6001 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     6061 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     6121 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     6181 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     6241 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     6301 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     6361 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     6421 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     6481 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     6541 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6601 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     6661 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     6721 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     6781 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     6841 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     6901 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     6961 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     7021 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     7081 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     7141 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     7201 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     7261 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     7321 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     7381 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     7441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     7501 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     7561 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7621 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7681 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7741 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7801 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7861 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     7921 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     7981 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8041 aggtgtatta ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8101 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8161 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8221 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8281 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8341 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8401 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8461 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8521 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     8581 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     8641 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     8701 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     8761 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     8821 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     8881 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     8941 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9001 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9061 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9121 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9181 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9241 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9301 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9361 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9421 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9481 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9541 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     9601 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     9661 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     9721 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     9781 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     9841 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     9901 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     9961 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc
    10021 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc
    10081 ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc
    10141 aaggcttgac cgacaattgc atgaagaatc tgcttagggt taggcgtttt gcgctgcttc
    10201 gcgatgtacg ggccagatat acgcgttgac attgattatt gactagttat taatagtaat
    10261 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
    10321 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
    10381 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
    10441 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
    10501 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
    10561 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
    10621 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
    10681 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
    10741 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
    10801 taagcagcgc gttttgcctg tactgggtct ctctggttag accagatctg agcctgggag
    10861 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
    10921 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
    10981 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
    11041 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
    11101 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
    11161 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
    11221 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
    11281 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
    11341 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
    11401 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
    11461 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
    11521 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
    11581 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
    11641 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
    11701 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
    11761 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
    11821 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
    11881 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
    11941 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
    12001 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
    12061 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
    12121 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
    12181 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
    12241 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
    12301 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
    12361 attcgattag tgaacggatc ggcactgcgt gcgccaattc tgcagacaaa tggcagtatt
    12421 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    12481 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    12541 aaattttcgg gtttattaca gggacagcag agatccagtt tggttaatta att
//
