LOCUS       sequence-238857-       14960 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses 3XFlag-tagged human CEP164 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14960)
  TITLE     Expresses 3xFlag-tagged human CEP164 in mammalian cells
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-EF1alpha-IRES-EGFP-3XFlag-hCEP164
FEATURES             Location/Qualifiers
     source          1..14960
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..92
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    99..123
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    join(4555..5129,5130..5132,5133..5141)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4722..4739)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4949..4968
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(5142..5144,5145..5147,5148..5861)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5142..5144,5145..5147,5148..5858)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5187..5208)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5448..5467)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5795..5816
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    5871..6459
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5924..5944)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6342..6353)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6983..7163
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7189..7206)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7195..7419
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7465..7893
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7552..7571)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7762..7783
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7902..7922)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7907..8236
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8087..8222
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8149..8168
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8284..8331
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8350..8724
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8854..8975
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8891..8910)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8945..8964
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9024..9040)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9024..9040)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9037..9059)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9048..9064
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9072..9078,9079..9096,9097..9102))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9117..9138
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9255..9272)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9426..10014)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9506..9525)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10185..10976,10977..11045))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10808..10827
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11046..11150)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11225..11244)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        11416..11795
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11797..11995
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     11950..11970
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             12013..12193
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    12240..12365
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12858..13091
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             13276..13320
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13618..13735
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        13782..14960
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          14013..14951
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     14908..14928
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 ggaattcggc cattacggcc accatggact acaaagacca tgacggtgat tataaagatc
       61 atgacatcga ttacaaggat gacgatgaca agctatcaac aagtttgtac aaaaaagcag
      121 gctccgcggc cgcccccttc accatggctg gacgacccct ccgcatagga gatcagctgg
      181 ttctggaaga agattatgat gagacctaca ttcctagtga gcaagaaatt cttgaatttg
      241 cccgggagat tggtattgat cccatcaagg aaccagaact gatgtggctg gcgcgagagg
      301 gcatcgtggc cccactgcct ggagagtgga aaccatgcca ggacatcaca ggtgacattt
      361 actatttcaa cttcgccaac gggcagtcta tgtgggacca tccatgtgac gaacactatc
      421 ggagcttggt gatccaagag cgggcaaagc tgtcaacttc tggggccatt aagaagaaga
      481 aaaaaaaaaa ggaaaagaaa gacaagaagg acagagaccc ccccaaaagt tcgctggcct
      541 tgggttcctc attagcccca gttcatgttc ctcttggggg cctggctcct ttacgaggtc
      601 ttgtggatac cccaccctct gctcttcgtg gatctcaaag cgtgagcctg gggagctcag
      661 tggagtctgg acgtcagctt ggagaactca tgctgccttc acagggtctc aagacctctg
      721 cttatacaaa gggtctcttg ggctccatat atgaggacaa gactgctctc agcctcttgg
      781 gtttaggaga agaaaccaat gaggaggatg aggaggaaag tgacaaccag agtgtccaca
      841 gctcaagtga gcctcttagg aacctacacc tggacattgg ggcactgggg ggtgactttg
      901 agtatgagga gtctctgaga acaagccagc cagaggagaa gaaggatgtt tctctggatt
      961 cagatgctgc cggtccccct actccctgca agccctccag cccaggtgca gacagcagtc
     1021 tgagcagtgc tgttggcaaa gggcgacagg gaagtggagc aagacctggt cttccagaaa
     1081 aagaggaaaa tgagaagagt gaacctaaga tttgcaggaa tctggtgacc cccaaggcag
     1141 accctacagg cagtgagcct gccaaagcct ctgaaaagga agcaccagag gacacagtag
     1201 atgcaggaga ggagggttcc aggagggaag aggcagccaa ggagccaaag aagaaggctt
     1261 ctgctctgga agagggcagt tcagacgcca gccaagaact ggaaattagt gaacacatga
     1321 aggaaccaca gctctcagac tccatagctt ctgaccccaa gtccttccat ggcctggact
     1381 tcggttttcg cagccggatc tcggagcacc tgctggatgt tgatgtgctt tccccagtcc
     1441 tgggtggagc ttgtcggcag gcccagcaac cactgggaat agaagacaag gatgacagcc
     1501 agtccagcca agatgagctg cagagcaagc agtccaaagg cctggaggag aggttatctc
     1561 ctccacttcc acacgaggag cgggcccaga gtccccctcg cagcctggcc actgaagaag
     1621 agcctcccca gggccccgag gggcagcccg agtggaagga ggcagaggag cttggggagg
     1681 actctgcagc cagcctcagc ctgcagctgt ccctccagag ggagcaggcc ccaagcccac
     1741 ctgctgcctg tgagaagggc aaggagcagc attcccaggc cgaggagctg ggccctgggc
     1801 aggaagaggc agaggatcct gaggagaagg tggcggtcag ccccaccccg ccagtctctc
     1861 cagaggtgcg atccacagag cctgtggctc ccccagagca gctctcagag gctgcactaa
     1921 aggccatgga agaggcagtg gcccaagtac tcgagcaaga ccagaggcac ctgctggaat
     1981 ccaagcaaga gaagatgcag caactgcggg agaagctgtg ccaagaggag gaagaggaga
     2041 tcctccggct tcaccagcag aaagagcaat ctctcagttc cttgagggag cggctgcaga
     2101 aagccattga ggaggaggag gcccggatga gagaggagga aagccagagg ctatcctggc
     2161 tccgagctca ggtccagtcc agcacacaag cagatgagga ccaaatcagg gctgagcaag
     2221 aggcttccct gcagaaactg agagaagagt tggagtctca acagaaggct gagagggcca
     2281 gcttggaaca gaaaaatagg caaatgctgg agcagctcaa ggaagagata gaggcttcgg
     2341 agaagagcga gcaggctgcc ctgaatgctg caaaggagaa ggctctgcag cagctgaggg
     2401 agcagctgga aggggagagg aaagaagctg tggcaacgct ggagaaggag cacagtgctg
     2461 agctggagcg gctctgctcc tcattggagg ccaagcaccg ggaggtggtc tccagcctcc
     2521 agaagaagat acaggaagct caacagaaag aggaggccca gctgcagaag tgccttgggc
     2581 aagtggagca cagagttcac cagaagtctt atcacgtggc tgggtatgag cacgagctca
     2641 gcagtctcct gcgagagaag cgccaggaag tggaagggga gcatgagagg aggttggaca
     2701 agatgaagga ggagcaccag caagtgatgg ctaaggccag agagcagtat gaagctgagg
     2761 agaggaagca gcgggctgag cttctggggc acctgaccgg agagctggag cgcctgcaga
     2821 gggcccatga acgagaactg gagactgtga ggcaggagca acacaagcgt cttgaggact
     2881 tgcggcgccg gcacagggag caggaaagga agctccagga tttagagttg gaccttgaaa
     2941 ccagagctaa agatgtcaag gccagattgg ctctgctgga ggtccaggag gagaccgccc
     3001 ggagggagaa gcagcagctg cttgatgtgc agaggcaggt tgctctgaag agtgaggaag
     3061 ccacagccac ccatcagcag ctggaggagg cacagaagga gcacacccac ctgttgcagt
     3121 caaaccagca gctccgagaa attcttgatg agctgcaggc ccgcaagctg aagctggagt
     3181 cccaagtgga tctgctgcag gctcagagcc agcaactgca gaaacacttc agcagcctgg
     3241 aggctgaagc tcaaaagaag cagcacctgt tgagagaagt gacagttgag gaaaataatg
     3301 cttccccaca ttttgagcca gatctccata ttgaggacct gaggaaatcc cttggaacaa
     3361 accagaccaa agaggtgtct tcttctctct cccagagcaa ggaggactta tacttggaca
     3421 gcctgtcctc ccacaatgtc tggcacctcc tctctgctga gggggtagcc ctccgtagtg
     3481 ccaaggagtt ccttgtgcag cagacacgct ccatgcggag gcggcagaca gctctgaaag
     3541 ctgcccagca gcattggcgc catgagctgg ccagtgcgca ggaggtggcc aaagacccac
     3601 caggcatcaa ggccctggaa gatatgcgca agaacctgga gaaggagacc aggcacctgg
     3661 atgagatgaa gtcggccatg cggaaaggcc acaacctgct gaagaagaaa gaggagaagc
     3721 tgaatcagtt ggagtcctct ctttgggaag aggcctcaga tgagggcact ctgggaggat
     3781 cccccaccaa gaaggcagta accttcgacc tcagtgacat ggacagcctg agcagtgaaa
     3841 gttctgaatc tttttccccg cctcaccgtg agtggtggcg gcagcagagg atcgactcaa
     3901 ccccgagtct cacctcccgc aagatccacg ggcttagcca ctccctccgg cagatcagca
     3961 gccagctgag cagtgtcctc agcatcctgg acagcctcaa ccctcagtcg ccgccgccgc
     4021 tcctcgcctc catgccagcc cagctccctc cccgggaccc taagagcacc cccaccccca
     4081 cctactatgg ctccctggcc aggttctcag ccttatcatc tgctacaccc acgtccaccc
     4141 aatgggcctg ggattcaggg caggggccca ggctcccctc ctctgtggct caaacggtgg
     4201 acgacttcct gttggagaag tggcgcaagt attttccatc tggcatcccg ctgctcagca
     4261 acagccccac cccgctggag agcaggctgg gttacatgtc tgccagtgag cagctccggc
     4321 tcctacagca ctcccattcg caagtccctg aggcgggcag caccaccttt cagggcataa
     4381 ttgaggccaa ccggaggtgg ctggaacgtg tcaagaatga ccccaggtta cctctcttct
     4441 cgtcaacacc caagccaaaa gctactttga gcctcctgca gctgggcctt gatgagcaca
     4501 acagagtgaa ggtgtatcgc ttctaaggcc gcctcggcca tcgataagga tccgcccctc
     4561 tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt
     4621 tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc
     4681 tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca
     4741 aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac
     4801 gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg
     4861 ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt
     4921 gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct
     4981 gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc ggtacacatg
     5041 ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt
     5101 ggttttcctt tgaaaaacac gatgataata tggccacaac catggtgagc aagggcgagg
     5161 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     5221 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     5281 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     5341 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     5401 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     5461 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     5521 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     5581 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     5641 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     5701 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     5761 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     5821 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc aatcaacctc
     5881 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5941 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     6001 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6061 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6121 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6181 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6241 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     6301 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6361 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6421 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgcctgcagg tttgtcgaga
     6481 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     6541 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     6601 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6661 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6721 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6781 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6841 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6901 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6961 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7021 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7081 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7141 gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc
     7201 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7261 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7321 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7381 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     7441 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7681 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7801 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7861 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     7921 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7981 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     8041 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     8101 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8161 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8221 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     8281 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     8341 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     8401 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     8461 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     8521 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     8581 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     8641 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     8701 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8761 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8821 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     9001 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     9061 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     9121 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     9181 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9241 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9301 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9361 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9421 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9481 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9541 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9601 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9661 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9721 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9781 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9841 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9901 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9961 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
    10021 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
    10081 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
    10141 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
    10201 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10261 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10321 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10381 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10441 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10501 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10561 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10621 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10681 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10741 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10801 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10861 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10921 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10981 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    11041 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    11101 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    11161 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    11221 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    11281 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    11341 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    11401 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
    11461 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    11521 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    11581 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    11641 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    11701 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    11761 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
    11821 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
    11881 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
    11941 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
    12001 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    12061 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    12121 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    12181 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
    12241 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
    12301 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
    12361 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
    12421 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
    12481 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
    12541 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
    12601 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
    12661 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
    12721 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
    12781 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
    12841 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
    12901 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
    12961 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
    13021 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
    13081 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
    13141 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
    13201 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
    13261 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
    13321 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
    13381 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
    13441 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
    13501 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
    13561 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
    13621 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
    13681 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
    13741 ttattacagg gacagcagag atccagtttg gttaattaat tggctccggt gcccgtcagt
    13801 gggcagagcg cacatcgccc acagtccccg agaagttgtg gggaggggtc ggcaattgaa
    13861 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
    13921 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
    13981 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
    14041 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
    14101 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
    14161 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
    14221 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
    14281 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
    14341 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
    14401 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
    14461 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
    14521 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
    14581 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
    14641 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
    14701 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
    14761 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
    14821 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
    14881 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
    14941 ttccatttca ggtgtcgtga
//
