LOCUS       sequence-238860-        7435 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for expression of gene of interest in Lactobacillus 
            plantarum and L. sakei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Sorvig E, Mathiesen G, Naterstad K, Eijsink VG, Axelsson L
  TITLE     High-level, inducible gene expression in Lactobacillus sakei and 
            Lactobacillus plantarum using versatile expression vectors.
  JOURNAL   Microbiology. 2005 Jul;151(Pt 7):2439-49. doi: 
            10.1099/mic.0.28084-0.
  PUBMED    16000734
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Microbiology."; date: "2005-07"; volume: "151(Pt 7):2439-49. doi"; 
            pages: " 10.1099/mic.0.28084-0"
COMMENT     Sequence Label: pSIP403
FEATURES             Location/Qualifiers
     source          1..7435
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             575..2383
                     /codon_start=1
                     /gene="uidA (or gusA) from E. coli"
                     /product="beta-glucuronidase"
                     /label=GUS
                     /translation="MVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
                     LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
                     TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
                     PKSAAFLLQKRWTGMNFGEKPQGGKQ"
     rep_origin      2731..3319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3220..3239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttaacct atctaatatt gccaattatg acagtaaaac acggagttta aaatttgtag
       61 atggcagtga ggcaaaagtc tcgttccgga aatcacggga actagtggcc aaattaaaac
      121 aaatgatgta gcgcctgcag cacgccaaat gatcccagta aaaagccacc cgcatggcgg
      181 gtggcttttt attagcccta gaagggcttc ccacacgcat ttcagcgcct tagtgcctta
      241 gtttgtgaat cataggtggt atagtcccga aatacccgtc taaggaattg tcagataggc
      301 ctaatgactg gcttttataa tatgagataa tgccgactgt actttttaca gtcggttttc
      361 taatgtcact aacctgcccc gttagttgaa gaaggttttt atattacagc tccagatcta
      421 ccggtgggcc catattaacg tttaaccgat aaagttgaac gttaatattt tttttgcgca
      481 gaaatggtaa attgaagcat aatagtcttg taaggtattt agctggctgg cgtaaagtat
      541 gctttataaa ataatatata ggagtatgat tcccatggta cgtcctgtag aaaccccaac
      601 ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg aaaactgtgg
      661 aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg ctgtgccagg
      721 cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca acgtctggta
      781 tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc tgcgtttcga
      841 tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg agcatcaggg
      901 cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga aaagtgtacg
      961 tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg gaatggtgat
     1021 taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta actatgccgg
     1081 aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg atatcaccgt
     1141 ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg tggtggccaa
     1201 tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa ctggacaagg
     1261 cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg aaggttatct
     1321 ctatgaactg tgcgtcacag ccaaaagcca gacagagtgt gatatctacc cgcttcgcgt
     1381 cggcatccgg tcagtggcag tgaagggcga acagttcctg attaaccaca aaccgttcta
     1441 ctttactggc tttggtcgtc atgaagatgc ggacttgcgt ggcaaaggat tcgataacgt
     1501 gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct accgtacctc
     1561 gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca tcgtggtgat
     1621 tgatgaaact gctgctgtcg gctttaacct ctctttaggc attggtttcg aagcgggcaa
     1681 caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc aagcgcactt
     1741 acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg tgatgtggag
     1801 tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg cgccactggc
     1861 ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg taatgttctg
     1921 cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga accgttatta
     1981 cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg aaaaagaact
     2041 tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg gcgtggatac
     2101 gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc agtgtgcatg
     2161 gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg aacaggtatg
     2221 gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta acaagaaagg
     2281 gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa aacgctggac
     2341 tggcatgaac ttcggtgaaa aaccgcaggg aggcaaacaa tgatctagac tcgaggaatt
     2401 cggtaccccg ggttcgaagg cgccaagctt caaattacag cacgtgttgc tttgattgat
     2461 agccaaaaag cagcagttga taaagcaatt actgatattg ctgaaaaatt gtaatttata
     2521 aataaaaatc accttttaga ggtggttttt ttatttataa attattcgtt tgatttcgct
     2581 ttcgatagaa caatcaaagc gagaataagg aagataaatc ccataagggc gggagcagaa
     2641 tgtccgagac taattcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2701 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2761 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2821 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2881 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2941 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3001 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3061 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     3301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     3361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     3421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gctgcgagtc
     3481 agtgagcgag aaggattatt cggctggttg agacgttaaa atgataaagg ttgtattaat
     3541 cttatattac ggttataatg tactcaactt aataaatgaa cgcaaaaaaa agaaccctca
     3601 acttagcaga gttaggattc acgacttatc agcacaacct gataagattt tcgatagcaa
     3661 gtactaccaa tacaagctat ctaacttggt actattataa catgtaggct aagtttttca
     3721 accattgata cttaaagtaa acggttgtta tcgggaatct taacagaaac ctgatagcaa
     3781 ccgttttttt gttattcaat ggttagcaac catcaaagca actaaaggct ggaaacctgt
     3841 tcttagctag taaaacctcc cgtgagtgtc gttcgtgacc ccgcttgcag ttaacaacat
     3901 aggtatgcta aaccttgtcg agatcaacgc gactaaagac gtggctggaa gactaggaaa
     3961 tgatacggac aggctaacta ttaacgcaga ttattcgggt tgctgctaaa accaactcta
     4021 ataatagtta gtgcaagggc tggttgagct taaattgtct gataaagagt tctctcttta
     4081 tactgcaaaa gaagcgcagt tattcacgat taggataact gtttgagaga gcctaagggc
     4141 ttgacccttg atggtttaag caccgctatg cgtgcgggat ccccttagaa gcaaacttag
     4201 agtgtgttga tagtgcatta tcttaaaatt ttgtataata ggaattgaag ttaaattaga
     4261 tgctaaaaat aggaattgaa gttaaattag atgctaaaaa tttgtaatta agaaggaggg
     4321 attcgtcatg ttggtattcc aaatgcgtaa tgtagataaa acatctactg ttttgaaaca
     4381 gactaaaaac agtgattacg cagataaata aatacgttag attaattcct accagtgact
     4441 aatcttatga ctttttaaac agataactaa aattacaaac aaatcgttta acttcaggag
     4501 agattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg aaaaagtact
     4561 caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg aaattggaac
     4621 aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa cgtctattga
     4681 attagacagt catctattca acttatcgtc agaaaaatta aaactgaata ctcgtgtcac
     4741 tttaattcac caagatattc tacagtttca attccctaac aaacagaggt ataaaattgt
     4801 tgggaatatt ccttacaatt taagcacaca aattattaaa aaagtggttt ttgaaagccg
     4861 tgcgtctgac atctatctga ctgttgaaga aggattctac aagcgtacct tggatattca
     4921 ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc ttaagctgcc
     4981 agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac ttacccgcca
     5041 taccacagat gttccagata aatattggaa gctatataag tactttgttt caaaatgggt
     5101 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt cgtcaagcaa tgaaacacgc
     5161 caaagtaaac aatttaagta ccattactta tgagcaagta ttgtctattt ttaatagtta
     5221 tctattattt aacgggagga aataattcta tgagtcgctt ttttaaattt ggaaagttac
     5281 acgttactaa agggaatgga gaccggggtc gacttcaata gagttcttaa cgttaatccg
     5341 aaaaaaacta acgttaatat taaaaaataa gatccgcttg tgaattatgt ataatttgat
     5401 tagactaaag aataggagaa agtatgatga tatttaaaaa actttctcgt taagataggt
     5461 tgttggtgag catgttatat acggatgtat cggtttcctt aatgcaaaat tttgttgcta
     5521 tcttattaat ttttctatta tatagatata ttcaaagaaa gataacattt aaacggatca
     5581 tattagatat tttaatagcg attatttttt caatattata tctgtttatt tcagatgcgt
     5641 cattacttgt aatggtatta atgcgattag ggtggcattt tcatcaacaa aaagaaaata
     5701 agataaaaac gactgataca gctaatttaa ttctaattat cgtgatccag ttattgttag
     5761 ttgcggttgg gactattatt agtcagttta ccatatcgat tatcaaaagt gatttcagcc
     5821 aaaatatatt gaacaatagt gcaacagata taactttatt aggtattttc tttgctgttt
     5881 tatttgacgg cttgttcttt atattattga agaataagcg gactgaatta caacatttaa
     5941 atcaagaaat cattgaattt tcgttagaaa aacaatattt tatatttata tttattttat
     6001 ttatagtaat agaaattatt ttagcagttg ggaatcttca aggagtaaca gccacgatat
     6061 tattaaccat tatcattatt ttttgtgtcc ttatcgggat gactttttgg caagtgatgc
     6121 tttttttgaa ggcttattcg attcgccaag aagccaatga ccaattggtc cggaatcaac
     6181 aacttcaaga ttatctagtc aatatcgaac agcagtacac cgaattacgg cgatttaagc
     6241 atgattatca aaacatctta ttatcgttgg agagttttgc cgaaaagggc gatcagcaac
     6301 agtttaaggc gtattaccaa gaattattag cacaacggcc aattcaaagt gaaatccaag
     6361 gggcagtcat tgcacaactc gactacttga aaaatgatcc tattcgagga ttagtcattc
     6421 aaaagttttt ggcagccaaa caggctggtg ttactttaaa attcgaaatg accgaaccaa
     6481 tcgaattagc aaccgctaat ctattaacgg ttattcggat tatcggtatt ttattagaca
     6541 atgcgattga acaagccgtt caagaaaccg atcaattggt gagttgtgct ttcttacaat
     6601 ctgatggttt aatcgaaatt acgattgaaa atacggccag tcaagttaag aatctccaag
     6661 cattttcaga gttaggctat tcaacgaaag gcgctggtcg ggggactggt ttagctaatg
     6721 tgcaggattt gattgccaaa caaaccaatt tattcttaga aacacagatt gaaaatagaa
     6781 agttacgaca gacattgatg attacggagg aaacttaatt tgtatcccgt ttatttatta
     6841 gaggatgatt tacagcaaca agcgatttat cagcaaatta tcgcgaatac gattatgatt
     6901 aacgaatttg caatgacttt aacatgcgct gccagtgata ctgagacatt gttggcggca
     6961 attaaggatc agcaacgagg tttattcttt ttggatatgg aaattgagga taaccgccaa
     7021 gccggtttag aagtggcaac taagattcgg cagatgatgc cgtttgcgca aattgtcttc
     7081 attacaaccc acgaggaact gacattatta acgttagaac gaaaaatagc gcctttagat
     7141 tacattctca aggaccaaac aatggctgaa atcaaaaggc aattgattga tgatctattg
     7201 ttagctgaga agcaaaacga ggcggcagcg tatcaccgag aaaatttatt tagttataaa
     7261 ataggtcctc gctttttctc attaccatta aaggaagttg tttatttata tactgaaaaa
     7321 gaaaatccgg gtcatattaa tttgttagcc gttaccagaa aggttacttt tccaggaaat
     7381 ttaaatgcgc tggaagccca atatccaatg ctctttcggt gtgataaaag ttact
//
