LOCUS       sequence-238861-        1338 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express the Helicobacter hepaticus specific TCRa/b HH5-4 in T cell 
            hybridoma cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1338)
  AUTHORS   Xu M, Pokrovskii M, Ding Y, Yi R, Au C, Harrison OJ, Galan C, 
            Belkaid Y, Bonneau R, Littman DR
  TITLE     c-MAF-dependent regulatory T cells mediate immunological tolerance 
            to a gut pathobiont.
  JOURNAL   Nature. 2018 Feb 15;554(7692):373-377. doi: 10.1038/nature25500. 
            Epub 2018 Feb 7.
  PUBMED    29414937
REFERENCE   2  (bases 1 to 1338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature25500"; journalName: "Nature"; date: "2018-02-15- 
            15"; volume: "554"; issue: "7692"; pages: "373-377"
COMMENT     Sequence Label: RV-mCD4-HH5-4
FEATURES             Location/Qualifiers
     source          1..1338
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      15..24
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             861..917
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gaaatttcct cgaggccacc atggacaaga ttctgacagc atcattttta ctcctaggcc
       61 ttcacctagc tggggtgaat ggccagcaga aggagaaaca tgaccagcag caggtgagac
      121 aaagtcccca atctctgaca gtctgggaag gaggaaccac agttctgacc tgcagttatg
      181 aggacagcac ttttaactac ttcccatggt accaacagtt ccctggggaa ggccctgcac
      241 ttctgatatc catactttca gtgtccgata aaaaggaaga tggacgattc acaaccttct
      301 tcaataaaag ggagaaaaag ctctccttgc acatcataga ctctcagcct ggagactcag
      361 ccacctactt ctgtgcagca agtccggctg gaggctataa agtggtcttt ggaagtggga
      421 ctcgattgct ggtaagccct gacatccaga acccagaacc tgctgtgtac cagttaaaag
      481 atcctcggtc tcaggacagc accctctgcc tgttcaccga ctttgactcc caaatcaatg
      541 tgccgaaaac catggaatct ggaacgttca tcactgacaa aactgtgctg gacatgaaag
      601 ctatggattc caagagcaat ggggccattg cctggagcaa ccagacaagc ttcacctgcc
      661 aagatatctt caaagagacc aacgccacct accccagttc agacgttccc tgtgatgcca
      721 cgttgactga gaaaagcttt gaaacagata tgaacctaaa ctttcaaaac ctgtcagtta
      781 tgggactccg aatcctcctg ctgaaagtag ccggatttaa cctgctcatg acgctgaggc
      841 tgtggtccag tggctccgga gccacgaact tctctctgtt aaagcaagca ggagacgtgg
      901 aagaaaaccc cggtcccatg tggcagtttt gcattctgtg cctctgtgta ctcatggctt
      961 ctgtggctac agaccccaca gtgactttgc tggagcaaaa cccaaggtgg cgtctggtac
     1021 cacgtggtca agctgtgaac ctacgctgca tcttgaaaaa ttcccagtat ccctggatga
     1081 gctggtatca gcaggatctc caaaagcaac tacagtggct gttcactctg cggagtcctg
     1141 gggacaaaga ggtcaaatct cttcccggtg ctgattacct ggccacacgg gtcactgata
     1201 cggagctgag gctgcaagtg gccaacatga gccagggcag aaccttgtac tgcacctgca
     1261 gttcccgaca ggggtcaaac acagaagtct tctttggtaa aggaaccaga ctcacagttg
     1321 tagaagatct ccggggcc
//
