LOCUS       sequence-238871-        2897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  clone CDA1 phage by SapI Golden Gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Thuronyi BW, Koblan LW, Levy JM, Yeh WH, Zheng C, Newby GA, Wilson 
            C, Bhaumik M, Shubina-Oleinik O, Holt JR, Liu DR
  TITLE     Continuous evolution of base editors with expanded target 
            compatibility and improved activity.
  JOURNAL   Nat Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0.
  PUBMED    31332326
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2019 Jul 22. pii: 10.1038/s41587-019-0193-0. doi: 
            10.1038/s41587-019-0193-0."
COMMENT     Sequence Label: pBT100.110
FEATURES             Location/Qualifiers
     source          1..2897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             34..42
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             47..670
                     /codon_start=1
                     /product="cytidine deaminase from the sea lamprey 
                     Petromyzon marinus (Rogozin et al., 2007)"
                     /label=PmCDA1
                     /translation="MTDAEYVRIHEKLDIYTFKKQFFNNKKSVSHRCYVLFELKRRGER
                     RACFWGYAVNKPQSGTERGIHAEIFSIRKVEEYLRDNPGQFTINWYSSWSPCADCAEKI
                     LEWYNQELRGNGHTLKIWACKLYYEKNARNQIGLWNLRDNGVGLNVMVSEHYQCCRKIF
                     IQSSHNQLNENRWLEKTLKRAEKRRSELSIMIQVKILHTTKSPAV"
     CDS             complement(1201..2016)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1201..2016)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1924..1943
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2008..2027
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2138..2726
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2627..2646
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2880..2897
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 aatgctcttc tagtctacta cataaatcaa acataaggag gtaaccatga ccgacgcgga
       61 atacgttcgt atccacgaaa aactggacat ctacaccttc aaaaaacagt tcttcaacaa
      121 caaaaaatct gtttctcacc gttgctacgt tctgttcgaa ctgaaacgtc gtggtgaacg
      181 tcgtgcgtgc ttctggggtt acgcggttaa caaaccgcag tctggtaccg aacgtggtat
      241 ccacgcggaa atcttctcta tccgtaaagt tgaagaatac ctgcgtgaca acccgggtca
      301 gttcaccatc aactggtact cttcttggtc tccgtgcgcg gactgcgcgg aaaaaatcct
      361 ggaatggtac aaccaggaac tgcgtggtaa cggtcacacc ctgaaaatct gggcgtgcaa
      421 actgtactac gaaaaaaacg cgcgtaacca gatcggtctg tggaacctgc gtgacaacgg
      481 tgttggtctg aacgttatgg tttctgaaca ctaccagtgc tgccgtaaaa tcttcatcca
      541 gtcttctcac aaccagctga acgaaaaccg ttggctggaa aaaaccctga aacgtgcgga
      601 aaaacgtcgt tctgaactgt ctatcatgat ccaggttaaa atcctgcaca ccaccaaatc
      661 tccggcggtt tccggtagcg aaacaccggg gacttcagaa tcggccaccc cggagtgcct
      721 gtcatacgaa accgagatac tgacagtaga atatggcctt ctgccaatcg ggaagattgt
      781 ggagaaacgg atagaatgca cagtttactc tgtcgataac aatggtaaca tttatactca
      841 gccagttgcc cagtggcacg accggggaga gcaggaagta ttcgaatact gtctggagga
      901 tggaagtctc attagggcca ctaaggacca caaatttatg acagtcgatg gccagatgct
      961 gcctatagac gaaatctttg agcgagagtt ggacctcatg cgagttgaca accttcctaa
     1021 ttaaggcggc tgaagagcct ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     1081 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     1141 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     1201 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     1261 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     1321 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     1381 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     1441 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     1501 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     1561 cgcctgagcc agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     1621 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     1681 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     1741 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     1801 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     1861 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     1921 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     1981 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     2041 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     2101 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2161 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2221 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2281 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2341 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2401 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2461 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2521 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2581 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2641 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2701 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2761 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2821 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2881 gcgagtcagt gagcgag
//
