LOCUS       sequence-238900-        4987 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for genomic integraction of the xylS regulator into the Tn7 
            insertion site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Volke DC, Turlin J, Mol V, Nikel PI
  TITLE     Physical decoupling of XylS/Pm regulatory elements and conditional 
            proteolysis enable precise control of gene expression in Pseudomonas
            putida.
  JOURNAL   Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383.
  PUBMED    30864281
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383."
COMMENT     Sequence Label: pTn7·xylS
FEATURES             Location/Qualifiers
     source          1..4987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..389
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     mobile_element  398..563
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     terminator      complement(577..663)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(729..1262)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1451..1456,1457..1473,1474..1479))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             complement(1504..2469)
                     /codon_start=1
                     /gene="xylS"
                     /product="XylS regulator encoded by the Pseudomonas putida 
                     TOL plasmid pWWO"
                     /label=XylS
                     /note="stimulates transcription from the Pm promoter in the
                     presence of aromatic compounds such as m-toluate or 
                     benzoate"
                     /translation="MDFCLLNEKSQIFVHAEPYAVSDYVNQYVGTHSIRLPKGGRPAGR
                     LHHRIFGCLDLCRISYGGSVRVISPGLETCYHLQIILKGHCLWRGHGQEHYFAPGELLL
                     LNPDDQADLTYSEDCEKFIVKLPSVVLDRACSDNNWHKPREGIRFAARHNLQQLDGFIN
                     LLGLVCDEAEHTKSMPRVQEHYAGIIASKLLEMLGSNVSREIFSKGNPSFERVVQFIEE
                     NLKRNISLERLAELAMMSPRSLYNLFEKHAGTTPKNYIRNRKLESIRACLNDPSANVRS
                     ITEIALDYGFLHLGRFAENYRSAFGELPSDTLRQCKKEVA"
     misc_feature    3370..3426
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     terminator      3469..3563
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(3865..3884)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3876..4691
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            4858..4966
                     /note="incP origin of transfer"
ORIGIN
        1 tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc
       61 tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaag gctttaaaag
      121 ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg
      181 atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag
      241 tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga
      301 gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat
      361 caacaggttg aactgctgat cttcagatcg gcgcgcctgt gggcggacaa aatagttggg
      421 aactgggagg ggtggaaatg gagtttttaa ggattattta gggaagagtg acaaaataga
      481 tgggaactgg gtgtagcgtc gtaagctaat acgaaaatta aaaatgacaa aatagtttgg
      541 aactagattt cacttatctg gtttctaggg cggcggattt gtcctactca ggagagcgtt
      601 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
      661 ttgatgccga cgtccaattt accgaacaac tccgcggccg ggaagccgat ctcggcttga
      721 acgaattgtt aggtggcggt acttgggtcg atatcaaagt gcatcacttc ttcccgtatg
      781 cccaactttg tatagagagc cactgcggga tcgtcaccgt aatctgcttg cacgtagatc
      841 acataagcac caagcgcgtt ggcctcatgc ttgaggagat tgatgagcgc ggtggcaatg
      901 ccctgcctcc ggtgctctcc ggagactgcg agatcataga tatagatctc actacgcggc
      961 tgctcaaact tgggcagaac gtaagccgcg agagcgccaa caaccgcttc ttggtcgaag
     1021 gcagcaagcg cgatgaatgt cttactacgg agcaagttcc cgaggtaatc ggagtccggc
     1081 tgatgttggg agtaggtggc tacgtctccg aactcacgac cgaaaagatc aagagcagcc
     1141 cgcatggatt tgacttggtc agggccgagc ctacatgtgc gaatgatgcc catacttgag
     1201 ccacctaact ttgttttagg gcgactgccc tgctgcgtaa catcgttgct gctgcgtaac
     1261 atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt gctgcttgga
     1321 tgcccgaggc ataggctgta caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     1381 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     1441 cttgcattac agtttacgaa ccgaacaggc ttatgtcaag acgtcttaat taaacgttcg
     1501 taatcaagcc acttcctttt tgcattgacg cagggtgtcg gaaggcaact cgccgaacgc
     1561 gctcctatag ttttcagcga agcgtcccaa atgtaagaag ccgtagtcta gggctatctc
     1621 agttatacta cgcacattgg cactgggatc gttcaagcag gcgcggatgc tttcgagctt
     1681 gcggttgcgg atgtagttct tcggcgtggt gccggcgtgc ttctcgaaca aattgtagag
     1741 cgagcgtgga ctcatcatcg ccagctccgc taaccgctca aggctgatat tccgtttgag
     1801 attctcctca atgaattgaa cgactcgctc gaaagacggg ttacctttgc tgaaaatttc
     1861 acggctgaca ttgctgccca gcatttcgag cagcttggaa gcgatgatcc ccgcatagtg
     1921 ctcttggacc cgaggcatcg actttgtatg ttccgcttcg tcacaaacta acccgagtag
     1981 attgataaag ccatcgagtt gctggagatt gtgtcgcgcg gcgaaacgga taccctccct
     2041 cggcttgtgc caattgttgt cactgcacgc ccgatcaagg accactgagg gcaatttaac
     2101 gataaatttc tcgcaatctt ctgaataggt caggtcggct tggtcatccg gattgagcag
     2161 caatagttcg cccggcgcaa aatagtgctc ctggccatgg ccacgccaca ggcaatggcc
     2221 tttgagtatt atttgcagat gataacaggt ctctaatcca ggcgagatta ccctcacgct
     2281 accgccgtag ctgattcgac acagatcgag gcatccgaag attctgtggt gcagcctgcc
     2341 tgccgggcgc ccgcccttgg gcaggcgaat agagtgcgta ccgacatact ggttaacata
     2401 atcggagact gcatagggct cggcgtggac gaagatctga cttttctcgt tcaataagca
     2461 aaaatccata gttcacggtt ctcttatttt aatgtgggct gcttggtgtg atgtagaaag
     2521 gcgccaagtc gatgaaaatg catctcgacg tgatgcgtat acgggttacc cccattgcca
     2581 cgttgcgcca tcctttttgc aatcagtgac cacttttcca agcaaaaata acgccaagca
     2641 gaacgaagac gttcttttta agaagcgaga acaccagaag ttcgtgctgt cggggcatgg
     2701 ggcgacgaat tggcggataa aggggatctg ctggatatta cggccttttt aaagaccgta
     2761 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     2821 gctcatccgt aattacgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2881 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2941 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     3001 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     3061 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     3121 cccgttttca ccatgcatgg gaattagctt gatctgacca acgaccggta gcggagctat
     3181 ccaacggcgg tataccagga aaacacacag caggtacatc agaacagtac catgactgaa
     3241 gaacaaatag ttttttcctg atccataaag cagaacggcc tgctccatga caaatctggc
     3301 tccccaacta atgccccatg cagccagcat aaccagcata aagtgcagtg tcctaggccg
     3361 cggccgcgcg aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc
     3421 aagcttgcgg ccgcgtcgtg actgggaaaa ccctggcgac tagtcttgga ctcctgttga
     3481 tagatccagt aatgacctca gaactccatc tggatttgtt cagaacgctc ggttgccgcc
     3541 gggcgttttt tattggtgag aatccagcca cgcccctctt taatacgacg ggcaatttgc
     3601 acttcagaaa atgaagagtt tgctttagcc ataacaaaag tccagtatgc tttttcacag
     3661 cataactgga ctgatttcag tttacaacta ttctgtctag tttaagactt tattgtcata
     3721 gtttagatct attttgttca gtttaagact ttattgtccg cccacaattt aaatttgtgt
     3781 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
     3841 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcag cgtgaaacga
     3901 gctgtagccg tccgcgtctg aacagcaaca tggatgcgga tctgtatggc tataaatggg
     3961 cgcgtgataa cgtgggtcag agcggcgcga ccatttatcg tctgtatggc aaaccggatg
     4021 cgccggaact gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg accgatgaaa
     4081 tggtgcgtct gaactggctg accgaattta tgccgctgcc gaccattaaa cattttattc
     4141 gcaccccgga tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc gcgtttcagg
     4201 tgctggaaga atatccggat agcggcgaaa acattgtgga tgcgctggcc gtgtttctgc
     4261 gtcgtctgca tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt gtgtttcgtc
     4321 tggcccaggc gcagagccgt atgaacaacg gcctggtgga tgcgagcgat tttgatgatg
     4381 aacgtaacgg ctggccggtg gaacaggtgt ggaaagaaat gcataaactg ctgccgttta
     4441 gcccggatag cgtggtgacc cacggcgatt ttagcctgga taacctgatt ttcgatgaag
     4501 gcaaactgat tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt tatcaggatc
     4561 tggccattct gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa cgtctgtttc
     4621 agaaatatgg cattgataat ccggatatga acaaactgca atttcatctg atgctggatg
     4681 aatttttcta ataattaatt ggaccgcggt ccgcgcgttg tccttttccg ctgcataacc
     4741 ctgcttcggg gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac
     4801 aggattttgc caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg
     4861 caggataggt gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc
     4921 gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccggcg
     4981 cgcccca
//
