LOCUS       sequence-238901-        6348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses truncated variant (aa367-aa714) of human FOXP2 in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6348)
  AUTHORS   Haussermann K, Young G, Kukura P, Dietz H
  TITLE     Dissecting FOXP2 Oligomerization and DNA Binding.
  JOURNAL   Angew Chem Int Ed Engl. 2019 Jun 3;58(23):7662-7667. doi: 
            10.1002/anie.201901734. Epub 2019 Apr 29.
  PUBMED    30887622
REFERENCE   2  (bases 1 to 6348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201901734"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2019-06-3- 3"; volume: "58"; issue: "23"; pages: "7662-7667"
COMMENT     Sequence Label: pET28b-FOXP2_P3
FEATURES             Location/Qualifiers
     source          1..6348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..74
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     158..177
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        158..176
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    177..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             216..238
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             257..274
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             284..301
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1361..1384
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(1440..1458)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1454..1501
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1538..1993
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1625..1644)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1835..1856
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2085..2900)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(2085..2900)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2808..2827
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2892..2911
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      3022..3610
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3511..3530
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3764..3781
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3796..3935
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3861..3880)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4021..4043
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(4037..4228)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(5037..6119)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     6081..6100
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(6120..6197)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
       61 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
      121 tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa
      181 ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata
      241 taccatgggc agcagccatc atcatcatca tcacagcagc ggcctggtgc cgcgcggcag
      301 ccatatgctt aacaatgaac acgcattgga tgaccgaagc actgctcagt gtcgagtgca
      361 aatgcaggtg gtgcaacagt tagaaataca gctttctaaa gaacgcgaac gtcttcaagc
      421 aatgatgacc cacttgcaca tgcgaccctc agagcccaaa ccatctccca aacctctaaa
      481 tctggtgtct agtgtcacca tgtcgaagaa tatgttggag acatccccac agagcttacc
      541 tcaaacccct accacaccaa cggccccagt caccccgatt acccagggac cctcagtaat
      601 caccccagcc agtgtgccca atgtgggagc catacgaagg cgacattcag acaaatacaa
      661 cattcccatg tcatcagaaa ttgccccaaa ctatgaattt tataaaaatg cagatgtcag
      721 acctccattt acttatgcaa ctctcataag gcaggctatc atggagtcat ctgacaggca
      781 gttaacactt aatgaaattt acagctggtt tacacggaca tttgcttact tcaggcgtaa
      841 tgcagcaact tggaagaatg cagtacgtca taatcttagc ctgcacaagt gttttgttcg
      901 agtagaaaat gttaaaggag cagtatggac tgtggatgaa gtagaatacc agaagcgaag
      961 gtcacaaaag ataacaggaa gtccaacctt agtaaaaaat atacctacca gtttaggcta
     1021 tggagcagct cttaatgcca gtttgcaggc tgccttggca gagagcagtt tacctttgct
     1081 aagtaatcct ggactgataa ataatgcatc cagtggccta ctgcaggccg tccacgaaga
     1141 cctcaatggt tctctggatc acattgacag caatggaaac agtagtccgg gctgctcacc
     1201 tcagccgcac atacattcaa tccacgtcaa ggaagagcca gtgattgcag aggatgaaga
     1261 ctgcccaatg tccttagtga caacagctaa tcacagtcca gaattagaag acgacagaga
     1321 gattgaagaa gagcctttat ctgaagatct ggaaagcgct tggagccacc cgcagttcga
     1381 aaaataatga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
     1441 cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt
     1501 gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta
     1561 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     1621 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     1681 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     1741 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     1801 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     1861 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     1921 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     1981 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2041 ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg
     2101 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     2161 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     2221 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     2281 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     2341 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     2401 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     2461 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     2521 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     2581 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     2641 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     2701 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     2761 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat
     2821 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt
     2881 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     2941 attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     3001 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     3061 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     3121 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     3181 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     3241 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     3301 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     3361 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     3421 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     3481 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     3541 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     3601 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     3661 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     3721 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     3781 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca
     3841 ccgcaatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca
     3901 ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg
     3961 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4021 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc
     4081 ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt
     4141 ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt
     4201 taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca
     4261 tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg
     4321 aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg
     4381 accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc
     4441 cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg
     4501 acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc
     4561 aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat
     4621 tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga
     4681 tcatgcgcac ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt
     4741 tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa
     4801 gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga
     4861 gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga
     4921 cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca
     4981 tcggtcgaga tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac
     5041 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     5101 cggggagagg cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg
     5161 ggcaacagct gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg
     5221 ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat
     5281 gagctgtctt cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg
     5341 gactcggtaa tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca
     5401 gtgggaacga tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc
     5461 cagtcgcctt cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag
     5521 ccagccagac gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc
     5581 tggtgaccca atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa
     5641 ataatactgt tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg
     5701 caggcagctt ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca
     5761 ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt
     5821 tctaccatcg acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg
     5881 acaatttgcg acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac
     5941 tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc
     6001 gcttccactt tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa
     6061 acggtctgat aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca
     6121 ttcaccaccc tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg
     6181 cgccattcga tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa
     6241 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
     6301 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accatacc
//
