LOCUS       sequence-238902-        3794 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP under the regulatory control of Pm. Expression needs 
            additional xyls gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Volke DC, Turlin J, Mol V, Nikel PI
  TITLE     Physical decoupling of XylS/Pm regulatory elements and conditional 
            proteolysis enable precise control of gene expression in Pseudomonas
            putida.
  JOURNAL   Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383.
  PUBMED    30864281
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383."
COMMENT     Sequence Label: pS23·Pm-GFP
FEATURES             Location/Qualifiers
     source          1..3794
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      456..1227
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     terminator      complement(1342..1428)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2260..2354
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(2475..2494)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2486..3301
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            3468..3576
                     /note="incP origin of transfer"
     CDS             complement(join(3587..3794,1..455))
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
ORIGIN
        1 cccggataca gggtcgggat gcggcgcagg tcgccatgcc ccaacagcga ttcgtcctgg
       61 tcgtcgtgat caaccaccac ggcggcactg aacaccgaca ggcgcaactg gtcgcggggc
      121 tggccccacg ccacgcggtc attgaccacg taggccgaca cggtgccggg gccgttgagc
      181 ttcacgacgg agatccagcg ctcggccacc aagtccttga ctgcgtattg gaccgtccgc
      241 aaagaacgtc cgatgagctt ggaaagtgtc ttctggctga ccaccacggc gttctggtgg
      301 cccatctgcg ccacgaggtg atgcagcagc attgccgccg tgggtttcct cgcaataagc
      361 ccggcccacg cctcatgcgc tttgcgttcc gtttgcaccc agtgaccggg cttgttcttg
      421 gcttgaatgc cgatttctct ggactgcgtg gccatgctta tctccatgcg gtaggggtgc
      481 cgcacggttg cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg acaacaatta
      541 tgcgttgcgt aaaagtggca gtcaattaca gattttcttt aacctacgca atgagctatt
      601 gcggggggtg ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt gatttttaag
      661 tctttcgcat ttcgccctat atctagttct ttggtgccca aagaagggca cccctgcggg
      721 gttcccccac gccttcggcg cggctccccc tccggcaaaa agtggcccct ccggggcttg
      781 ttgatcgact gcgcggcctt cggccttgcc caaggtggcg ctgccccctt ggaacccccg
      841 cactcgccgc cgtgaggctc ggggggcagg cgggcgggct tcgcccttcg actgccccca
      901 ctcgcatagg cttgggtcgt tccaggcgcg tcaaggccaa gccgctgcgc ggtcgctgcg
      961 cgagccttga cccgccttcc acttggtgtc caaccggcaa gcgaagcgcg caggccgcag
     1021 gccggaggct tttccccaga gaaaattaaa aaaattgatg gggcaaggcc gcaggccgcg
     1081 cagttggagc cggtgggtat gtggtcgaag gctgggtagc cggtgggcaa tccctgtggt
     1141 caagctcgtg ggcaggcgca gcctgtccat cagcttgtcc agcagggttg tccacgggcc
     1201 gagcgaagcg agccagccgg tggccgctcg cggccatcgt ccacatatcc acgggctggc
     1261 aagggagcgc agcgaccgcg cagggcgaag cccggagagc aagcccgtag ggggggcgcg
     1321 cccagctgtc tagggcggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca
     1381 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctttaatt
     1441 aattgcaaga agcggataca ggagtgcaaa aaatggctat ctctagtaag gcctacccct
     1501 taggctttat gcaagcttag gaggaaaaac atatgcgtaa aggtgaagaa ctgttcaccg
     1561 gtgttgttcc gatcctggtt gaactggatg gtgatgttaa cggccacaaa ttctctgttc
     1621 gtggtgaagg tgaaggtgat gcaaccaacg gtaaactgac cctgaaattc atctgcacta
     1681 ccggtaaact gccggttcca tggccgactc tggtgactac cctgacctat ggtgttcagt
     1741 gtttttctcg ttacccggat cacatgaagc agcatgattt cttcaaatct gcaatgccgg
     1801 aaggttatgt acaggagcgc accatttctt tcaaagacga tggcacctac aaaacccgtg
     1861 cagaggttaa atttgaaggt gatactctgg tgaaccgtat tgaactgaaa ggcattgatt
     1921 tcaaagagga cggcaacatc ctgggccaca aactggaata taacttcaac tcccataacg
     1981 tttacatcac cgcagacaaa cagaagaacg gtatcaaagc taacttcaaa attcgccata
     2041 acgttgaaga cggtagcgta cagctggcgg accactacca gcagaacact ccgatcggtg
     2101 atggtccggt tctgctgccg gataaccact acctgtccac ccagtctaaa ctgtccaaag
     2161 acccgaacga aaagcgcgac cacatggtgc tgctggagtt cgttactgcc gcaggtatca
     2221 cgcacggcat ggatgaactc tacaaataaa ctagtcttgg actcctgttg atagatccag
     2281 taatgacctc agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt
     2341 ttattggtga gaatccaggg gtccccaata attacgattt aaatttgtgt ctcaaaatct
     2401 ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac tgtctgctta
     2461 cataaacagt aatacaaggg gtgttatgag ccatattcag cgtgaaacga gctgtagccg
     2521 tccgcgtctg aacagcaaca tggatgcgga tctgtatggc tataaatggg cgcgtgataa
     2581 cgtgggtcag agcggcgcga ccatttatcg tctgtatggc aaaccggatg cgccggaact
     2641 gtttctgaaa catggcaaag gcagcgtggc gaacgatgtg accgatgaaa tggtgcgtct
     2701 gaactggctg accgaattta tgccgctgcc gaccattaaa cattttattc gcaccccgga
     2761 tgatgcgtgg ctgctgacca ccgcgattcc gggcaaaacc gcgtttcagg tgctggaaga
     2821 atatccggat agcggcgaaa acattgtgga tgcgctggcc gtgtttctgc gtcgtctgca
     2881 tagcattccg gtgtgcaact gcccgtttaa cagcgatcgt gtgtttcgtc tggcccaggc
     2941 gcagagccgt atgaacaacg gcctggtgga tgcgagcgat tttgatgatg aacgtaacgg
     3001 ctggccggtg gaacaggtgt ggaaagaaat gcataaactg ctgccgttta gcccggatag
     3061 cgtggtgacc cacggcgatt ttagcctgga taacctgatt ttcgatgaag gcaaactgat
     3121 tggctgcatt gatgtgggcc gtgtgggcat tgcggatcgt tatcaggatc tggccattct
     3181 gtggaactgc ctgggcgaat ttagcccgag cctgcaaaaa cgtctgtttc agaaatatgg
     3241 cattgataat ccggatatga acaaactgca atttcatctg atgctggatg aatttttcta
     3301 ataattaatt ggaccgcggt ccgcgcgttg tccttttccg ctgcataacc ctgcttcggg
     3361 gtcattatag cgattttttc ggtatatcca tcctttttcg cacgatatac aggattttgc
     3421 caaagggttc gtgtagactt tccttggtgt atccaacggc gtcagccggg caggataggt
     3481 gaagtaggcc cacccgcgag cgggtgttcc ttcttcactg tcccttattc gcacctggcg
     3541 gtgctcaacg ggaatcctgc tctgcgaggc tggccgtagg ccggccctac cggcgcggca
     3601 gcgttacccg tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg
     3661 gcatcggcag gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca
     3721 ggtctggttc catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg
     3781 gtagttgctg ctcg
//
