LOCUS       sequence-238905-        5940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses truncated variant (aa503-aa714) of human FOXP2 in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5940)
  AUTHORS   Haussermann K, Young G, Kukura P, Dietz H
  TITLE     Dissecting FOXP2 Oligomerization and DNA Binding.
  JOURNAL   Angew Chem Int Ed Engl. 2019 Jun 3;58(23):7662-7667. doi: 
            10.1002/anie.201901734. Epub 2019 Apr 29.
  PUBMED    30887622
REFERENCE   2  (bases 1 to 5940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/anie.201901734"; journalName: "Angew Chem Int Ed Engl"; 
            date: "2019-06-3- 3"; volume: "58"; issue: "23"; pages: "7662-7667"
COMMENT     Sequence Label: pET28b-FOXP2_P4
FEATURES             Location/Qualifiers
     source          1..5940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..91
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     175..194
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        175..193
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    194..218
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             233..255
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             274..291
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             301..318
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             970..993
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     primer_bind     complement(1049..1067)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1063..1110
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1147..1602
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1234..1253)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1444..1465
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(1694..2509)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(1694..2509)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2417..2436
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2501..2520
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      2631..3219
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3120..3139
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3373..3390
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    3405..3544
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(3470..3489)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3630..3652
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(3646..3837)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(4646..5728)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5690..5709
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5729..5806)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
       61 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
      121 atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaattaatac
      181 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
      241 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
      301 ctggtgccgc gcggcagcca tatggtcaga cctccattta cttatgcaac tctcataagg
      361 caggctatca tggagtcatc tgacaggcag ttaacactta atgaaattta cagctggttt
      421 acacggacat ttgcttactt caggcgtaat gcagcaactt ggaagaatgc agtacgtcat
      481 aatcttagcc tgcacaagtg ttttgttcga gtagaaaatg ttaaaggagc agtatggact
      541 gtggatgaag tagaatacca gaagcgaagg tcacaaaaga taacaggaag tccaacctta
      601 gtaaaaaata tacctaccag tttaggctat ggagcagctc ttaatgccag tttgcaggct
      661 gccttggcag agagcagttt acctttgcta agtaatcctg gactgataaa taatgcatcc
      721 agtggcctac tgcaggccgt ccacgaagac ctcaatggtt ctctggatca cattgacagc
      781 aatggaaaca gtagtccggg ctgctcacct cagccgcaca tacattcaat ccacgtcaag
      841 gaagagccag tgattgcaga ggatgaagac tgcccaatgt ccttagtgac aacagctaat
      901 cacagtccag aattagaaga cgacagagag attgaagaag agcctttatc tgaagatctg
      961 gaaagcgctt ggagccaccc gcagttcgaa aaataatgag atccggctgc taacaaagcc
     1021 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     1081 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     1141 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     1201 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     1261 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     1321 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     1381 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     1441 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     1501 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     1561 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     1621 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1681 catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1741 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1801 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1861 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1921 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     1981 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     2041 tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa ggacaattac
     2101 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     2161 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     2221 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     2281 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     2341 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     2401 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     2461 aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg
     2521 tattactgtt tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag
     2581 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     2641 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     2701 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     2761 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     2821 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     2881 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     2941 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     3001 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     3061 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     3121 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     3181 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     3241 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     3301 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     3361 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     3421 ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct
     3481 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     3541 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     3601 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     3661 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     3721 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     3781 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     3841 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     3901 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     3961 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     4021 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     4081 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     4141 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     4201 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     4261 tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc
     4321 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     4381 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     4441 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     4501 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     4561 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
     4621 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     4681 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca
     4741 gggtggtttt tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc
     4801 cctgagagag ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt
     4861 tgatggtggt taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta
     4921 ccgagatatc cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg
     4981 ccatctgatc gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca
     5041 tggtttgttg aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa
     5101 tttgattgcg agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac
     5161 ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc
     5221 ccagtcgcgt accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga
     5281 catcaagaaa taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt
     5341 catccagcgg atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg
     5401 ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca
     5461 gttgatcggc gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac
     5521 tggaggtggc aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt
     5581 tgggaatgta attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa
     5641 cgtggctggc ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg
     5701 cgacatcgta taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc
     5761 gctatcatgc cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc
     5821 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     5881 accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
//
