LOCUS       sequence-238909-       12838 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a dominant negative Kash construct that disrupts LINC 
            complexes in addition to fluorescently tagged (mRuby2) 
            alpha-Actinin-2 on an adenoviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12838)
  AUTHORS   Seelbinder B, Ghosh S, Schneider SE, Scott AK, Berman AG, Goergen 
            CJ, Margulies KB, Bedi KC Jr, Casas E, Swearingen AR, Brumbaugh J, 
            Calve S, Neu CP
  TITLE     Nuclear deformation guides chromatin reorganization in cardiac 
            development and disease.
  JOURNAL   Nat Biomed Eng. 2021 Dec;5(12):1500-1516. doi: 
            10.1038/s41551-021-00823-9. Epub 2021 Dec 2.
  PUBMED    34857921
REFERENCE   2  (bases 1 to 12838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-021-00823-9"; journalName: "Nat Biomed Eng"; date: 
            "2021-12"; volume: "5"; issue: "12"; pages: "1500-1516"
COMMENT     Sequence Label: Ad:2CMV_A2R2-N25K3
FEATURES             Location/Qualifiers
     source          1..12838
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    107..228
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     enhancer        326..629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        630..833
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3575..4285
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4447..4568
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7759..7861
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7950..7967)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8121..8709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8201..8220)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9541..10335
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9541..10335
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9595..9614)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10205..10224
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(10383..10763)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10445..10466)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10657..10676
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   10801..10903
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10991..11141
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        11199..11502
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11503..11706
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             11828..12559
                     /codon_start=1
                     /product="brighter version of the far-red fluorescent 
                     protein mNeptune2 with slightly blue-shifted spectra"
                     /label=mNeptune2.5
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
       61 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
      121 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
      181 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaac gcggatctgg
      241 gcgtggttaa tatttcatag cgcgtaatac tgtaatagta atcaattacg gggtcattag
      301 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      361 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      421 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      481 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      541 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      601 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      661 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      721 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      781 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
      841 tgaaccgtca gatccgctag aggctagcac catgaatcag atagagcccg gcgtgcagta
      901 caactatgtc tacgacgaag atgagtatat gatccaggag gaggagtggg accgcgacct
      961 gctcctcgac ccggcctggg agaagcagca gaggaagacg ttcactgcct ggtgcaactc
     1021 acacctgcgg aaagctggca cccagatcga gaacatcgag gaggatttca ggaacggcct
     1081 caagctcatg ctacttctag aagtcatctc aggggaaagg ctgcccaaac ctgaccgggg
     1141 aaaaatgcgg ttccacaaga ttgcgaatgt caacaaggct ctggattaca tagccagcaa
     1201 gggagtgaag ctggtatcta ttggagctga agaaatagtc gatggcaatg tgaagatgac
     1261 cctgggtatg atctggacca tcatcctccg cttcgccatt caggacatct cagtggaaga
     1321 aacatctgcc aaagaagggc tgctgctatg gtgtcagagg aagacagctc catacagaaa
     1381 tgtgaatatc cagaacttcc acaccagctg gaaagatggc cttggactct gtgccctcat
     1441 ccacagacac cggcccgacc tcattgacta ctcaaagctt aacaaggatg accccatagg
     1501 aaacattaac ctggccatgg aaatagcaga gaagcacttg gatatcccta agatgttgga
     1561 tgctgaagac attgtcaaca ctcccaaacc cgatgaaaga gccatcatga cttacgtttc
     1621 ctgcttctac catgctttcg cgggagcgga gcaggccgag acagcagcta acagaatatg
     1681 taaggttctt gctgtgaatc aagagaatga gaggctgatg gaagaatatg agaggctagc
     1741 gagtgagctg ttggagtgga ttcggcgcac aatcccctgg ctggagaaca ggacgcctga
     1801 gaagaccatg caggccatgc agaagaagct ggaggacttc cgggattacc gtcgtaagca
     1861 taagcccccc aaggtgcagg agaagtgcca gctggagatc aacttcaaca cgctgcagac
     1921 caagcttcgg atcagcaacc ggcccgcctt catgccctcc gagggcaaga tggtgtcgga
     1981 cattgctggt gcctggcaga ggttggagca ggctgagaag ggctatgagg agtggctatt
     2041 gaatgagatt cgaagactgg agcgcttgga gcacttggct gagaagttca ggcagaaggc
     2101 ctccacgcac gagacatggg cttatggcaa agaacagatc ttgctgcaga aggactacga
     2161 gtctgcgtcc ctgacggaag ttcgagccct acttcggaag cacgaggcct ttgagagcga
     2221 cctggctgca caccaggacc gcgtggagca gatcgctgcc attgcgcagg agctcaatga
     2281 actggactat cacgatgctg tgaacgtcaa tgatcggtgc cagaaaattt gcgaccagtg
     2341 ggatagattg gggacgctca cccagaagag gagggaggcc ttggagagaa ctgagaagtt
     2401 gctggaaaca atcgaccaac tgcacctgga gtttgccaag agagccgcgc ccttcaacaa
     2461 ctggatggag ggagccatgg aggacctgca ggacatgttc attgtccaca gcatcgagga
     2521 gatccagagt ctgatcactg cacatgagca gttcaaggcc acactgcccg aggccgacgg
     2581 ggagcggcag tccatcctgg ccatccagaa cgaggtggag aaggtgatcc agagctacag
     2641 catccggatc agctccagca acccctacag cactgtcacc atggatgagc tgcgcaacaa
     2701 gtgggacaag gtgaagcagc tggtgccggt ccgggatcag tccctgcagg aggagctggc
     2761 tcgccaacat gctaatgagc gtctgcggcg ccagtttgct gctcaggcca atgccattgg
     2821 gccctggatc cagaacaaga tggaggagat cgctcggagc tccatccaga tcaccggggc
     2881 cctggaggac cagatgaacc agctgaagca gtacgaacac aacatcatca actacaagaa
     2941 caacatcgac aagctggagg gcgaccacca gctcatccag gaggccctgg tcttcgacaa
     3001 caagcacacg aactacacca tggagcatat ccgtgtcgga tgggagttgc tgctgacaac
     3061 tatcgccaga accatcaatg aggtggagac ccagatcctg acaagagatg ccaagggcat
     3121 cacccaggag cagatgaatg agttcagagc ctccttcaac cactttgaca ggaggaagaa
     3181 cggcctgatg gatcatgagg atttcagagc ctgcctcatt tccatgggct atgacttggg
     3241 tgaagctgaa tttgcccgca ttatgacttt ggttgacccc aacggacaag gcacggtcac
     3301 cttccagtcc ttcattgact tcatgactag agagaccgca gacaccgaca cggcagagca
     3361 ggtcatcgcc tccttccgga ttctggcttc tgataagcct tacatcttgg cagaggagct
     3421 tcgtcgagag ctgcctccgg atcaggccca gtactgcatc aagagaatgc ccccatactc
     3481 aggcccgggc agtgtccccg gggccctgga ctacactgcc ttctcctctg ccctctacgg
     3541 ggaaagcgac ctcggcgacc ctcctgttgc aacgatggtg tctaagggcg aagagctgat
     3601 caaggaaaat atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa
     3661 atgcacaggt gaaggagaag gcaatccgta catgggaact caaaccatga ggatcaaagt
     3721 catcgaggga ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg
     3781 cagccgtact tttatcaagt acccgaaagg cattcctgat ttctttaaac agtcctttcc
     3841 tgagggtttt acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcat
     3901 gcaggacacc agccttgagg atggctgtct cgtttaccac gtccaagtca gaggggtaaa
     3961 ctttccctcc aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga
     4021 gatgatgtat ccagcagatg gtggtctgag gggatacact catatggcac tgaaagttga
     4081 tggtggtggc catctgtctt gctctttcgt aacaacttac aggtcaaaaa agaccgtcgg
     4141 gaacatcaag atgcccggta tccatgccgt tgatcaccgc ctggaaaggt tagaggaaag
     4201 tgacaatgaa atgttcgtag tacaacgcga acacgcagtt gccaagttcg ccgggcttgg
     4261 tggtgggatg gacgagctgt acaagtaact cgagcctaag cttctagata agatatccga
     4321 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt
     4381 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
     4441 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     4501 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     4561 gtatcttaac gcggatctgg gcgtggttaa gggtgggaaa gaatatataa ggtgggggtc
     4621 ttatgtagtt ttgtatctgt tttgcagcag ccgccgccgc catgagcacc aactcgtttg
     4681 atggaagcat tgtgagctca tatttgacaa cgcgcatgcc cccatgggcc ggggtgcgtc
     4741 agaatgtgat gggctccagc attgatggtc gccccgtcct gcccgcaaac tctactacct
     4801 tgacctacga gaccgtgtct ggaacgccgt tggagactgc agcctccgcc gccgcttcag
     4861 ccgctgcagc caccgcccgc gggattgtga ctgactttgc tttcctgagc ccgcttgcaa
     4921 gcagtgcagc ttcccgttca tccgcccgcg atgacaagtt gacggctctt ttggcacaat
     4981 tggattcttt gacccgggaa cttaatgtcg tttctcagca gctgttggat ctgcgccagc
     5041 aggtttctgc cctgaaggct tcctcccctc ccaatgcggt ttaaaacata aataaaaaac
     5101 cagactctgt ttggatttgg atcaagcaag tgtcttgctg tctttattta ggggttttgc
     5161 gcgcgcggta ggcccgggac cagcggtctc ggtcgttgag ggtcctgtgt attttttcca
     5221 ggacgtggta aaggtgactc tggatgttca gatacatggg cataagcccg tctctggggt
     5281 ggaggtagca ccactgcaga gcttcatgct gcggggtggt gttgtagatg atccagtcgt
     5341 agcaggagcg ctgggcgtgg tgcctaaaaa tgtctttcag tagcaagctg attgccaggg
     5401 gcaggccctt ggtgtaagtg tttacaaagc ggttaagctg ggatgggtgc atacgtgggg
     5461 atatgagatg catcttggac tgtattttta ggttggctat gttcccagcc atatccctcc
     5521 ggggattcat gttgtgcaga accaccagca cagtgtatcc ggtgcacttg ggaaatttgt
     5581 catgtagctt agaaggaaat gcgtggaaga acttggagac gcccttgtga cctccaagat
     5641 tttccatgca ttcgtccata atgatggcaa tgggcccacg ggcggcggcc tgggcgaaga
     5701 tatttctggg atcactaacg tcatagttgt gttccaggat gagatcgtca taggccattt
     5761 ttacaaagcg cgggcggagg gtgccagact gcggtataat ggttccatcc ggcccagggg
     5821 cgtagttacc ctcacagatt tgcatttccc acgctttgag ttcagatggg gggatcatgt
     5881 ctacctgcgg ggcgatgaag aaaacggttt ccggggtagg ggagatcagc tgggaagaaa
     5941 gcaggttcct gagcagctgc gacttaccgc agccggtggg cccgtaaatc acacctatta
     6001 ccggctgcaa ctggtagtta agagagctgc agctgccgtc atccctgagc aggggggcca
     6061 cttcgttaag catgtccctg actcgcatgt tttccctgac caaatccgcc agaaggcgct
     6121 cgccgcccag cgatagcagt tcttgcaagg aagcaaagtt tttcaacggt ttgagaccgt
     6181 ccgccgtagg catgcttttg agcgtttgac caagcagttc caggcggtcc cacagctcgg
     6241 tcacctgctc tacggcatct cgatccagca tatctcctcg tttcgcgggt tggggcggct
     6301 ttcgctgtac ggcagtagtc ggtgctcgtc cagacgggcc agggtcatgt ctttccacgg
     6361 gcgcagggtc ctcgtcagcg tagtctgggt cacggtgaag gggtgcgctc cgggctgcgc
     6421 gctggccagg gtgcgcttga ggctggtcct gctggtgctg aagcgctgcc ggtcttcgcc
     6481 ctgcgcgtcg gccaggtagc atttgaccat ggtgtcatag tccagcccct ccgcggcgtg
     6541 gcccttggcg cgcagcttgc ccttggagga ggcgccgcac gaggggcagt gcagactttt
     6601 gagggcgtag agcttgggcg cgagaaatac cgattccggg gagtaggcat ccgcgccgca
     6661 ggccccgcag acggtctcgc attccacgag ccaggtgagc tctggccgtt cggggtcaaa
     6721 aaccaggttt cccccatgct ttttgatgcg tttcttacct ctggtttcca tgagccggtg
     6781 tccacgctcg gtgacgaaaa ggctgtccgt gtccccgtat acagacttga gagggagttt
     6841 aaacgaattc aatagcttgt tgcatgggcg gcgatataaa atgcaaggtg ctgctcaaaa
     6901 aatcaggcaa agcctcgcgc aaaaaagaaa gcacatcgta gtcatgctca tgcagataaa
     6961 ggcaggtaag ctccggaacc accacagaaa aagacaccat ttttctctca aacatgtctg
     7021 cgggtttctg cataaacaca aaataaaata acaaaaaaac atttaaacat tagaagcctg
     7081 tcttacaaca ggaaaaacaa cccttataag cataagacgg actacggcca tgccggcgtg
     7141 accgtaaaaa aactggtcac cgtgattaaa aagcaccacc gacagctcct cggtcatgtc
     7201 cggagtcata atgtaagact cggtaaacac atcaggttga ttcacatcgg tcagtgctaa
     7261 aaagcgaccg aaatagcccg ggggaataca tacccgcagg cgtagagaca acattacagc
     7321 ccccatagga ggtataacaa aattaatagg agagaaaaac acataaacac ctgaaaaacc
     7381 ctcctgccta ggcaaaatag caccctcccg ctccagaaca acatacagcg cttccacagc
     7441 ggcagccata acagtcagcc ttaccagtaa aaaagaaaac ctattaaaaa aacaccactc
     7501 gacacggcac cagctcaatc agtcacagtg taaaaaaggg ccaagtgcag agcgagtata
     7561 tataggacta aaaaatgacg taacggttaa agtccacaaa aaacacccag aaaaccgcac
     7621 gcgaacctac gcccagaaac gaaagccaaa aaacccacaa cttcctcaaa tcgtcacttc
     7681 cgttttccca cgttacgtca cttcccattt taagaaaact acaattccca acacatacaa
     7741 gttactccgc cctaaaacct acgtcacccg ccccgttccc acgccccgcg ccacgtcaca
     7801 aactccaccc cctcattatc atattggctt caatccaaaa taaggtatat tattgatgat
     7861 gttaattaac atgcatggat ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     7921 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8281 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     8341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8521 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9181 gttgttgcca ttgctgcagc catgagatta tcaaaaagga tcttcaccta gatccttttc
     9241 acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag ctactgggct
     9301 atctggacaa gggaaaacgc aagcgcaaag agaaagcagg tagcttgcag tgggcttaca
     9361 tggcgatagc tagactgggc ggttttatgg acagcaagcg aaccggaatt gccagctggg
     9421 gcgccctctg gtaaggttgg gaagccctgc aaagtaaact ggatggcttt cttgccgcca
     9481 aggatctgat ggcgcagggg atcaagctct gatcaagaga caggatgagg atcgtttcgc
     9541 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     9601 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     9661 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     9721 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     9781 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     9841 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     9901 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     9961 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
    10021 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
    10081 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
    10141 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
    10201 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
    10261 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
    10321 gacgagttct tctgaatttt gttaaaattt ttgttaaatc agctcatttt ttaaccaata
    10381 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
    10441 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
    10501 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
    10561 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
    10621 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
    10681 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
    10741 taatgcgccg ctacagggcg cgtccattcg ccattcagga tcgaattaat tcttaattaa
    10801 catcatcaat aatatacctt attttggatt gaagccaata tgataatgag ggggtggagt
    10861 ttgtgacgtg gcgcggggcg tgggaacggg gcgggtgacg tagtagtgtg gcggaagtgt
    10921 gatgttgcaa gtgtggcgga acacatgtaa gcgacggatg tggcaaaagt gacgtttttg
    10981 gtgtgcgccg gtgtacacag gaagtgacaa ttttcgcgcg gttttaggcg gatgttgtag
    11041 taaatttggg cgtaaccgag taagatttgg ccattttcgc gggaaaactg aataagagga
    11101 agtgaaatct gaataatttt gtgttactca tagcgcgtaa tactgtaata gtaatcaatt
    11161 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
    11221 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
    11281 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
    11341 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
    11401 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
    11461 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
    11521 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
    11581 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
    11641 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
    11701 agagctggtt tagtgaaccg tcagatccgc tagagatctg gtaccgtcga cgcggccgct
    11761 cgacaccatg aaactaggac gggccgtgct gggcctgctg ctgctggcgc cgtccgtggt
    11821 gcaggcgatg gtgagcaagg gcgaggagct gatcaaggag aacatgcaca cgaagctgta
    11881 catggaaggc accgtgaaca accaccactt caagtgcacc cacgaagggg agggcaagcc
    11941 ctacgagggc acccagacca acaggattaa ggtggtggag ggaggccccc tgccgttcgc
    12001 attcgacatc ctggccacct gctttatgta cgggagcaag accttcatca accacaccca
    12061 gggcatcccc gatttcttta agcagtcctt ccctgagggc ttcacatggg agagagtcac
    12121 cacatacgaa gacgggggcg tgcttaccgt tacccaggac accagcctcc aggacggctg
    12181 cttgatctac aacgtcaagc tcagaggggt gaacttccca tccaacggcc ctgtgatgca
    12241 gaagaaaaca ctcggctggg aggccagtac cgagaccctg taccccgctg acggcggcct
    12301 ggaaggcaga tgcgacatgg ccctgaagct cgtgggcggg ggccacctgc actgcaacct
    12361 gaagaccaca tacagatcca agaaacccgc taagaacctc aagatgcccg gcgtctactt
    12421 tgtggaccgc agactggaaa gaatcaagga ggccgacaat gagacctacg tcgagcagca
    12481 cgaggtggct gtggccagat actgcgacct ccctagcaaa ctggggcaca aacttaatgg
    12541 catggacgag ctgtacaagg gggatccacc ggtcgccacc agtcggaggc agaagcggtg
    12601 gagtccctgc tctctcctac agaaagcatg ccgtgtggca ctgccattgc agctgttgct
    12661 cctgctcttt ctgctgctgc tgttcctgct gccggccggc gaggaggagc gcagctgcgc
    12721 cctggccaac aacttcgccc gctcctttgc gctcatgctt cggtacaatg gccccccgcc
    12781 cacctagggt accctaagct tctagataag atatccgatc caccggatct agataact
//
