LOCUS       sequence-238938-       10825 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Self-assebled 24-mer tagged with iLID, and EGFP. Upon blue-light 
            activation can bind up to 24 SspB-modified proteins..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10825)
  AUTHORS   Bracha D, Walls MT, Wei MT, Zhu L, Kurian M, Avalos JL, Toettcher 
            JE, Brangwynne CP
  TITLE     Mapping Local and Global Liquid Phase Behavior in Living Cells Using
            Photo-Oligomerizable Seeds.
  JOURNAL   Cell. 2018 Nov 29;175(6):1467-1480.e13. doi: 
            10.1016/j.cell.2018.10.048.
  PUBMED    30500534
REFERENCE   2  (bases 1 to 10825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2018.10"; journalName: "Cell"; date: "2018-11-29- 
            29"; volume: "175"; issue: "6"; pages: "1467-1480.e13"
COMMENT     Sequence Label: M43: pHR-SFFVp-iLID::EGFP::FTH1
FEATURES             Location/Qualifiers
     source          1..10825
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        85..492
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             572..1009
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             1040..1753
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1070..1098)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1693..1713
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     misc_feature    2454..3042
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2507..2527)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2925..2936)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3045..3061)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3046..3062)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3152..3385
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3410..3428)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3411..3428)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3718..3737)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3951..3970)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4070..4092
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4130..4148)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4216..4320
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4321..4389,4390..5181)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4539..4558)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5352..5940
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5841..5860
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6094..6111
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6181..6201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6186..6515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6366..6501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7649..7714
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7844..7864
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8289..8423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8326..8345)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8380..8399
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8591..9224
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9271..9396
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9893..10126
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10311..10355
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10640..10757
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ccgataaaat aaaagatttt atttagtctc cagaaaaagg ggggaatgaa agaccccacc
       61 tgtaggtttg gcaagctagc tgcagtaacg ccattttgca aggcatggaa aaataccaaa
      121 ccaagaatag agaagttcag atcaagggcg ggtacatgaa aatagctaac gttgggccaa
      181 acaggatatc tgcggtgagc agtttcggcc ccggcccggg gccaagaaca gatggtcacc
      241 gcagtttcgg ccccggcccg aggccaagaa cagatggtcc ccagatatgg cccaaccctc
      301 agcagtttct taagacccat cagatgtttc caggctcccc caaggacctg aaatgaccct
      361 gcgccttatt tgaattaacc aatcagcctg cttctcgctt ctgttcgcgc gcttctgctt
      421 cccgagctct ataaaagagc tcacaacccc tcactcggcg cgccagtcct ccgacagact
      481 gagtcgcccg ggggggatct ggagctctcg agaattctca cgcgtcaagt ggagcaaggc
      541 aggtggacag tggatcacta gtgccaccat gctggcaacc acactggaac ggatcgagaa
      601 aaatttcgtg attactgatc cgagactgcc tgacaaccca atcatttttg cgagcgattc
      661 cttcctgcag ctgacagaat attctcggga agagatcctg gggcgcaatt gccgttttct
      721 gcagggaccc gagacagacc gtgccactgt tcggaaaatc agagatgcta ttgacaacca
      781 gactgaagtg accgttcagc tgatcaatta taccaagagc ggcaagaagt tctggaacgt
      841 gttccacctg cagccgatgc gcgattataa gggcgacgtc cagtacttca ttggcgtgca
      901 gctggatggc accgaacgtc ttcatggcgc cgctgagcgt gaggcggtct gcctgatcaa
      961 aaagacagcc tttcagattg ctgaggcagc gaacgacgaa aattactttg gcggctccgg
     1021 ctctggatcc ggagctacca tgagtaaagg agaagaactt ttcactggag ttgtcccaat
     1081 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
     1141 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
     1201 tgttccatgg ccaacacttg tcactaccct gacttatggt gttcaatgct tttcaagata
     1261 cccagatcat atgaaacggc atgacttttt caagagtgcc atgcccgaag gttatgtaca
     1321 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
     1381 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
     1441 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
     1501 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
     1561 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
     1621 tttaccagac aaccattacc tgtccacaca atctaagctt tcgaaagatc ccaacgaaaa
     1681 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
     1741 tgaactatac aaaggtggat ctggaggttc aggtggaagt gcggccggta ccggcagcgg
     1801 aggaatgacg accgcgtcca cctcgcaggt gcgccagaac taccaccagg actcagaggc
     1861 cgccatcaac cgccagatca acctggagct ctacgcctcc tacgtttacc tgtccatgtc
     1921 ttactacttt gaccgcgatg atgtggcttt gaagaacttt gccaaatact ttcttcacca
     1981 atctcatgag gagagggaac atgctgagaa actgatgaag ctgcagaacc aacgaggtgg
     2041 ccgaatcttc cttcaggata tcaagaaacc agactgtgat gactgggaga gcgggctgaa
     2101 tgcaatggag tgtgcattac atttggaaaa aaatgtgaat cagtcactac tggaactgca
     2161 caaactggcc actgacaaaa atgaccccca tttgtgtgac ttcattgaga cacattacct
     2221 gaatgagcag gtgaaagcca tcaaagaatt gggtgaccac gtgaccaact tgcgcaagat
     2281 gggagcgccc gaatctggct tggcggaata tctctttgac aagcacaccc tgggagacag
     2341 tgataatgaa agctgagcgg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     2401 gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac
     2461 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     2521 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     2581 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     2641 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     2701 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     2761 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     2821 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     2881 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     2941 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     3001 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     3061 gagggaatta attcgagctc ggtagcttta agaccaatga cttacaaggc agctgtagat
     3121 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     3181 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     3241 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     3301 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     3361 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
     3421 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     3481 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     3541 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     3601 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
     3661 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     3721 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     3781 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     3841 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     3901 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     3961 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     4021 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     4081 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     4141 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     4201 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4261 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4321 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4381 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4441 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4501 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4561 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4621 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     4681 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     4741 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     4801 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     4861 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     4921 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     4981 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5041 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5101 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5161 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5221 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     5281 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     5341 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     5401 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     5461 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     5521 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     5581 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     5641 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     5701 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     5761 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     5821 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     5881 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     5941 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     6001 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     6061 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     6121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     6181 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     6241 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     6301 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     6361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6421 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6481 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     6541 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     6601 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     6661 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     6721 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     6781 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     6841 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     6901 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     6961 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     7021 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     7081 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     7141 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     7201 ttgttctttt taacttcagg cgtgttacaa tagtttccag taagtattct ggaggctgca
     7261 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     7321 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     7381 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     7441 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     7501 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     7561 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     7621 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     7681 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     7741 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     7801 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     7861 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     7921 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     7981 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     8041 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     8101 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt
     8161 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac
     8221 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     8281 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     8341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     8401 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     8461 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     8521 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     8581 acttagtagt tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg
     8641 atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc
     8701 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta
     8761 gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg
     8821 gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac
     8881 gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag
     8941 ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga
     9001 gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga
     9061 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     9121 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     9181 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     9241 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     9301 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     9361 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     9421 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     9481 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     9541 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     9601 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     9661 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     9721 accgcacagc aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     9781 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     9841 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     9901 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     9961 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
    10021 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
    10081 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
    10141 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
    10201 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
    10261 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
    10321 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
    10381 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
    10441 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
    10501 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
    10561 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa
    10621 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10681 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10741 caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata
    10801 agcttgatat cgaattcctg cagcc
//
