LOCUS       sequence-238947-        4211 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses GFP under the regulatory control of Pm. Expression needs 
            additional xyls gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Volke DC, Turlin J, Mol V, Nikel PI
  TITLE     Physical decoupling of XylS/Pm regulatory elements and conditional 
            proteolysis enable precise control of gene expression in Pseudomonas
            putida.
  JOURNAL   Microb Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383.
  PUBMED    30864281
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb 
            Biotechnol. 2019 Mar 12. doi: 10.1111/1751-7915.13383."
COMMENT     Sequence Label: pS24·Pm-GFP
FEATURES             Location/Qualifiers
     source          1..4211
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      822..916
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     complement(1037..1056)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1048..1863
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     oriT            2030..2138
                     /note="incP origin of transfer"
     rep_origin      2232..2820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2721..2740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(2874..3707)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      3721..4072
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
ORIGIN
        1 ttaattgcaa gaagcggata caggagtgca aaaaatggct atctctagta aggcctaccc
       61 cttaggcttt atgcaagctt aggaggaaaa acatatgcgt aaaggtgaag aactgttcac
      121 cggtgttgtt ccgatcctgg ttgaactgga tggtgatgtt aacggccaca aattctctgt
      181 tcgtggtgaa ggtgaaggtg atgcaaccaa cggtaaactg accctgaaat tcatctgcac
      241 taccggtaaa ctgccggttc catggccgac tctggtgact accctgacct atggtgttca
      301 gtgtttttct cgttacccgg atcacatgaa gcagcatgat ttcttcaaat ctgcaatgcc
      361 ggaaggttat gtacaggagc gcaccatttc tttcaaagac gatggcacct acaaaacccg
      421 tgcagaggtt aaatttgaag gtgatactct ggtgaaccgt attgaactga aaggcattga
      481 tttcaaagag gacggcaaca tcctgggcca caaactggaa tataacttca actcccataa
      541 cgtttacatc accgcagaca aacagaagaa cggtatcaaa gctaacttca aaattcgcca
      601 taacgttgaa gacggtagcg tacagctggc ggaccactac cagcagaaca ctccgatcgg
      661 tgatggtccg gttctgctgc cggataacca ctacctgtcc acccagtcta aactgtccaa
      721 agacccgaac gaaaagcgcg accacatggt gctgctggag ttcgttactg ccgcaggtat
      781 cacgcacggc atggatgaac tctacaaata aactagtctt ggactcctgt tgatagatcc
      841 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
      901 ttttattggt gagaatccag gggtccccaa taattacgat ttaaatttgt gtctcaaaat
      961 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct
     1021 tacataaaca gtaatacaag gggtgttatg agccatattc agcgtgaaac gagctgtagc
     1081 cgtccgcgtc tgaacagcaa catggatgcg gatctgtatg gctataaatg ggcgcgtgat
     1141 aacgtgggtc agagcggcgc gaccatttat cgtctgtatg gcaaaccgga tgcgccggaa
     1201 ctgtttctga aacatggcaa aggcagcgtg gcgaacgatg tgaccgatga aatggtgcgt
     1261 ctgaactggc tgaccgaatt tatgccgctg ccgaccatta aacattttat tcgcaccccg
     1321 gatgatgcgt ggctgctgac caccgcgatt ccgggcaaaa ccgcgtttca ggtgctggaa
     1381 gaatatccgg atagcggcga aaacattgtg gatgcgctgg ccgtgtttct gcgtcgtctg
     1441 catagcattc cggtgtgcaa ctgcccgttt aacagcgatc gtgtgtttcg tctggcccag
     1501 gcgcagagcc gtatgaacaa cggcctggtg gatgcgagcg attttgatga tgaacgtaac
     1561 ggctggccgg tggaacaggt gtggaaagaa atgcataaac tgctgccgtt tagcccggat
     1621 agcgtggtga cccacggcga ttttagcctg gataacctga ttttcgatga aggcaaactg
     1681 attggctgca ttgatgtggg ccgtgtgggc attgcggatc gttatcagga tctggccatt
     1741 ctgtggaact gcctgggcga atttagcccg agcctgcaaa aacgtctgtt tcagaaatat
     1801 ggcattgata atccggatat gaacaaactg caatttcatc tgatgctgga tgaatttttc
     1861 taataattaa ttggaccgcg gtccgcgcgt tgtccttttc cgctgcataa ccctgcttcg
     1921 gggtcattat agcgattttt tcggtatatc catccttttt cgcacgatat acaggatttt
     1981 gccaaagggt tcgtgtagac tttccttggt gtatccaacg gcgtcagccg ggcaggatag
     2041 gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac tgtcccttat tcgcacctgg
     2101 cggtgctcaa cgggaatcct gctctgcgag gctggccgta ggccggccga taatctcatg
     2161 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     2221 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     2281 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     2341 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     2401 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     2461 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     2521 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     2581 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2641 cttcccgaag ggagaaaggc ggacaggcat ccggtaagcg gcagggtcgg aacaggagag
     2701 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2761 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     2821 aacgccagca acgcggccgt gaaaggcagg ccggtccgtg gtggccacgg cctctaggcc
     2881 agatccagcg gcatctgggt tagtcgagcg cgggccgctt cccatgtctc accagggcga
     2941 gcctgtttcg cgatctcagc atctgaaatc ttcccggcct tgcgcttcgc tggggcctta
     3001 cccaccgcct tggcgggctt cttcggtcca aaactgaaca acagatgtgt gaccttgcgc
     3061 ccggtctttc gctgcgccca ctccacctgt agcgggctgt gctcgttgat ctgcgtcacg
     3121 gctggatcaa gcactcgcaa cttgaagtcc ttgatcgagg gataccggcc ttccagttga
     3181 aaccactttc gcagctggtc aatttctatt tcgcgctggc cgatgctgtc ccattgcatg
     3241 agcagctcgt aaagcctgat cgcgtgggtg ctgtccatct tggccacgtc agccaaggcg
     3301 tatttggtga actgtttggt gagttccgtc aggtacggca gcatgtcttt ggtgaacctg
     3361 agttctacac ggccctcacc ctcccggtag atgattgttt gcacccagcc ggtaatcatc
     3421 acactcggtc ttttcccctt gccattgggc tcttgggtta accggacttc ccgccgtttc
     3481 aggcgcaggg ccgcttcttt gagctggttg taggaagatt cgatagggac acccgccatc
     3541 gtcgctatgt cctccgccgt cactgaatac atcacttcat cggtgacagg ctcgctcctc
     3601 ttcacctggc taatacaggc cagaacgatc cgctgttcct gaacactgag gcgatacgcg
     3661 gcctcgacca gggcattgct tttgtaaacc attgggggtg aggccacgtt cgacattcct
     3721 tgtgtataag gggacactgt atctgcgtcc cacaatacaa caaatccgtc cctttacaac
     3781 aacaaatccg tcccttctta acaacaaatc cgtcccttaa tggcaacaaa tccgtccctt
     3841 tttaaactct acaggccacg gattacgtgg cctgtagacg tcctaaaagg tttaaaaggg
     3901 aaaaggaaga aaagggtgga aacgcaaaaa acgcaccact acgtggcccc gttggggccg
     3961 catttgtgcc cctgaagggg cgggggaggc gtctgggcaa tccccgtttt accagtcccc
     4021 tatcgccgcc tgagagggcg caggaagcga gtaatcaggg tatcgaggcg gattcaccct
     4081 tggcgtccaa ccagcggcac cagcggcgcc tgagaggggc gcgcccagct gtctagggcg
     4141 gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa cgaaaggccc
     4201 agtctttcga c
//
