LOCUS       sequence-238964-       11537 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses genes for lycopene synthesis (crtI, crtB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Chatzivasileiou AO, Ward V, Edgar SM, Stephanopoulos G
  TITLE     Two-step pathway for isoprenoid synthesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 
            10.1073/pnas.1812935116.
  PUBMED    30584096
REFERENCE   2  (bases 1 to 11537)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2018 Dec 24. pii: 1812935116. doi: 
            10.1073/pnas.1812935116."
COMMENT     Sequence Label: p5T7-LYCipi-ggpps
FEATURES             Location/Qualifiers
     source          1..11537
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      889..1111
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(1834..2916)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2878..2897
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(2917..2994)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     3040..3059
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3040..3058
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3059..3083
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8507..8551
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(8589..8607)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8603..8650
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(9385..10176)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(join(11428..11537,1..841))
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 attttgcctc aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc
       61 ttagtccgtt atgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca
      121 tttttatctg gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt
      181 ggaaaatcaa cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt
      241 aagtgtttaa atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat
      301 ggtagttatt ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg
      361 ccttgtgagt tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt
      421 atttgttttc aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga
      481 aaagataagg caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg
      541 catagtttgt ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag
      601 ttctcgtcat cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga
      661 tgttcatcat ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag
      721 ggttttcaat cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct
      781 ccgttaagtc atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca
      841 tacatctcaa ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat
      901 gataattact agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg
      961 ctggaaaact tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt
     1021 tttttgttta tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa
     1081 aaagaataga tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca
     1141 aaaggatgtc gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct
     1201 taagtagcac cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc
     1261 aggcacctga gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca
     1321 gtgaatgggg gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc
     1381 ataatacaag aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt
     1441 ggtgctatct gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca
     1501 cttcggatta tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt
     1561 atcatcaaca ggcttacccg tcttactgtc gggaattcgc gttggccgat tcattaatgc
     1621 agctggcacg acaggtttcc tctagatttc agtgcaattt atctcttcaa atgtagcacc
     1681 tgaagtcagc cccatacgat ataagttgta attctcatgt tagtcatgcc ccgcgcccac
     1741 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     1801 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1861 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1921 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     1981 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     2041 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     2101 tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     2161 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     2221 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     2281 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     2341 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     2401 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     2461 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     2521 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     2581 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     2641 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     2701 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     2761 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     2821 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     2881 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     2941 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     3001 ctcgacgctc tcccttatgc gactcctgca ttaggaaatt aatacgactc actatagggg
     3061 aattgtgagc ggataacaat tcccctgtag aaataatttt gtttaacttt aataaggaga
     3121 tataccatat gttcgacttc aacgagtaca tgaaatcgaa agcagttgca gttgatgctg
     3181 cgcttgacaa agcgattccg ctggaatacc ctgaaaagat tcacgaatcg atgcgttata
     3241 gtctgctggc tggtggcaaa cgcgtgcgcc cagctctttg cattgcggca tgtgagctgg
     3301 taggcggttc ccaggatctg gctatgccaa cggcgtgcgc aatggaaatg atccatacaa
     3361 tgtccctgat ccacgatgat ctgccgtgta tggataatga tgacttccgc cgtggaaaac
     3421 cgactaacca taaagtattt ggcgaggaca ctgcagtgtt ggcaggagac gccctgttga
     3481 gctttgcctt tgaacatatt gccgtcgcga cctcaaaaac agttccttct gatcgtaccc
     3541 tgcgcgtcat cagtgagtta ggtaagacca ttggcagcca ggggctggta ggcggccagg
     3601 tcgtggatat cacgtctgaa ggtgacgcga atgtggatct taagacctta gagtggattc
     3661 acatccataa aacggccgtg ctgctggaat gctcggttgt gtccggtggg atcctggggg
     3721 gcgccactga ggacgaaatc gcccgtattc gccgttatgc acggtgtgtg ggcctcttgt
     3781 ttcaagtcgt ggatgatatt ctggatgtga cgaaatctag tgaggagctc ggtaaaaccg
     3841 cgggcaagga tctcctgacc gacaaggcga cgtacccgaa actgatgggt ttggaaaagg
     3901 ctaaggagtt tgctgccgaa ttagcgacca gagccaaaga agaactctct tctttcgacc
     3961 agatcaaggc agcgcccctt ttagggctgg cggattatat tgcctttcgt caaaactaat
     4021 accgcccttt tgggttcaag cagtacataa cgatggaacc acattacagg agtagtgatg
     4081 aatgaaggac gagcgccttg ttcagcgtaa gaacgatcat ctggatatcg ttctcgaccc
     4141 ccgtcgcgcc gtaactcagg ctagcgcagg ttttgagcgc tggcgcttta cccactgcgc
     4201 cctgccagag ctgaatttta gcgacatcac gctggaaacc accttcctga atcggcagct
     4261 acaggctccg ctgctgatca gctccatgac cggcggcgtt gagcgctcgc gccatatcaa
     4321 ccgccacctc gccgaggcgg cgcaggtgct aaaaattgcg atgggggtgg gctcccagcg
     4381 cgtcgccatt gagagcgacg cgggcttagg gctggataaa accctgcggc agctggctcc
     4441 ggacgtgccg ctgctggcga acctcggcgc ggcgcagctg accggcagaa aaggtattga
     4501 ttacgcccga cgggccgtgg agatgatcga ggcggatgcg ctgattgtgc acctaaaccc
     4561 gctgcaggag gcgctacagc ccggcggcga tcgcgactgg cgcggacggc tggcggctat
     4621 tgaaactctg gtccgcgagc tgcccgttcc gctggtggtg aaagaggtgg gagccggtat
     4681 ctcccgaacc gtggccgggc agctgatcga tgccggcgtt accgtgattg acgtcgcggg
     4741 cgcgggcggc accagctggg ccgccgttga aggcgagcgg gcggccaccg agcagcagcg
     4801 cagcgtggcc aacgtctttg ccgacatcgt tctcgacccc cgtcgcgccg taactcaggc
     4861 tagcgcaggt tttgagcgct ggcgctttac ccactgcgcc ctgccagagc tgaattttag
     4921 cgacatcacg ctggaaacca ccttcctgaa tcggcagcta caggctccgc tgctgatcag
     4981 ctccatgacc ggcggcgttg agcgctcgcg ccatatcaac cgccacctcg ccgaggcggc
     5041 gcaggtgcta aaaattgcga tgggggtggg ctcccagcgc gtcgccattg agagcgacgc
     5101 gggcttaggg ctggataaaa ccctgcggca gctggctccg gacgtgccgc tgctggcgaa
     5161 cctcggcgcg gcgcagctga ccggcagaaa aggtattgat tacgcccgac gggccgtgga
     5221 gatgatcgag gcggatgcgc tgattgtgca cctaaacccg ctgcaggagg cgctacagcc
     5281 cggcggcgat cgcgactggc gcggacggct ggcggctatt gaaactctgg tccgcgagct
     5341 gcccgttccg ctggtggtga aagaggtggg agccggtatc tcccgaaccg tggccgggca
     5401 gctgatcgat gccggcgtta ccgtgattga cgtcgcgggc gcgggcggca ccagctgggc
     5461 cgccgttgaa ggcgagcggg cggccaccga gcagcagcgc agcgtggcca acgtctttgc
     5521 cgactggggg atccccaccg ctgaggcgct ggttgacatt gccgaggcct ggccgcagat
     5581 gccccttatt gcctcgggaa tgcgggctgg gctatttcac cctaccactg gctattcgct
     5641 gccgctggcg gtggcccttg ccgacgcgat tgccgacagc ccgcggctgg gcagcgttcc
     5701 gctctatcag ctcacccggc agtttgccga acgccactgg cgcaggcagg gattcttccg
     5761 cctgctgaac cggatgcttt tcctggccgg gcgcgaggag aaccgctggc gggtgatgca
     5821 gcgcttttat gggctgccgg agcccaccgt agagcgcttt tacgccggtc ggctctctct
     5881 ctttgataag gcccgcattt tgacgggcaa gccaccggtt ccgctgggcg aagcctggcg
     5941 ggcggcgctg aaccattttc ctgacagacg agataaagga tgaaaaaaac cgttgtgatt
     6001 ggcgcaggct ttggtggcct ggcgctggcg attcgcctgc aggcggcagg gatcccaacc
     6061 gtactgctgg agcagcggga caagcccggc ggtcgggcct acgtctggca tgaccagggc
     6121 tttacctttg acgccgggcc gacggtgatc accgatccta ccgcgcttga ggcgctgttc
     6181 accctggccg gcaggcgcat ggaggattac gtcaggctgc tgccggtaaa acccttctac
     6241 cgactctgct gggagtccgg gaagaccctc gactatgcta acgacagcgc cgagcttgag
     6301 gcgcagatta cccagttcaa cccccgcgac gtcgagggct accggcgctt tctggcttac
     6361 tcccaggcgg tattccagga gggatatttg cgcctcggca gcgtgccgtt cctctctttt
     6421 cgcgacatgc tgcgcgccgg gccgcagctg cttaagctcc aggcgtggca gagcgtctac
     6481 cagtcggttt cgcgctttat tgaggatgag catctgcggc aggccttctc gttccactcc
     6541 ctgctggtag gcggcaaccc cttcaccacc tcgtccatct acaccctgat ccacgccctt
     6601 gagcgggagt ggggggtctg gttccctgag ggcggcaccg gggcgctggt gaacggcatg
     6661 gtgaagctgt ttaccgatct gggcggggag atcgaactca acgcccgggt cgaagagctg
     6721 gtggtggccg ataaccgcgt aagccaggtc cggctggcgg atggtcggat ctttgacacc
     6781 gacgccgtag cctcgaacgc tgacgtggtg aacacctata aaaagctgct cggccaccat
     6841 ccggtggggc agaagcgggc ggcagcgctg gagcgcaaga gcatgagcaa ctcgctgttt
     6901 gtgctctact tcggcctgaa ccagcctcat tcccagctgg cgcaccatac catctgtttt
     6961 ggtccccgct accgggagct gatcgacgag atctttaccg gcagcgcgct ggcggatgac
     7021 ttctcgctct acctgcactc gccctgcgtg accgatccct cgctcgcgcc tcccggctgc
     7081 gccagcttct acgtgctggc cccggtgccg catcttggca acgcgccgct ggactgggcg
     7141 caggaggggc cgaagctgcg cgaccgcatc tttgactacc ttgaagagcg ctatatgccc
     7201 ggcctgcgta gccagctggt gacccagcgg atctttaccc cggcagactt ccacgacacg
     7261 ctggatgcgc atctgggatc ggccttctcc atcgagccgc tgctgaccca aagcgcctgg
     7321 ttccgcccgc acaaccgcga cagcgacatt gccaacctct acctggtggg cgcaggtact
     7381 caccctgggg cgggcattcc tggcgtagtg gcctcggcga aagccaccgc cagcctgatg
     7441 attgaggatc tgcaatgagc caaccgccgc tgcttgacca cgccacgcag accatggcca
     7501 acggctcgaa aagttttgcc accgctgcga agctgttcga cccggccacc cgccgtagcg
     7561 tgctgatgct ctacacctgg tgccgccact gcgatgacgt cattgacgac cagacccacg
     7621 gcttcgccag cgaggccgcg gcggaggagg aggccaccca gcgcctggcc cggctgcgca
     7681 cgctgaccct ggcggcgttt gaaggggccg agatgcagga tccggccttc gctgcctttc
     7741 aggaggtggc gctgacccac ggtattacgc cccgcatggc gctcgatcac ctcgacggct
     7801 ttgcgatgga cgtggctcag acccgctatg tcacctttga ggatacgctg cgctactgct
     7861 atcacgtggc gggcgtggtg ggtctgatga tggccagggt gatgggcgtg cgggatgagc
     7921 gggtgctgga tcgcgcctgc gatctggggc tggccttcca gctgacgaat atcgcccggg
     7981 atattattga cgatgcggct attgaccgct gctatctgcc cgccgagtgg ctgcaggatg
     8041 ccgggctgac cccggagaac tatgccgcgc gggagaatcg ggccgcgctg gcgcgggtgg
     8101 cggagcggct tattgatgcc gcagagccgt actacatctc ctcccaggcc gggctacacg
     8161 atctgccgcc gcgctgcgcc tgggcgatcg ccaccgcccg cagcgtctac cgggagatcg
     8221 gtattaaggt aaaagcggcg ggaggcagcg cctgggatcg ccgccagcac accagcaaag
     8281 gtgaaaaaat tgccatgctg atggcggcac cggggcaggt tattcgggcg aagacgacga
     8341 gggtgacgcc gcgtccggcc ggtctttggc agcgtcccgt ttaggcgggc ggccatgacg
     8401 ttcacgcagt attagttaag tataagaagg agatatacat atggcagatc tcaattggat
     8461 atcggccggc cacgcgatcg ctgacgtcgg taccctcgag tctggtaaag aaaccgctgc
     8521 tgcgaaattt gaacgccagc acatggactc gtctactagc gcagcttaat taacctaggc
     8581 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     8641 gggttttttg ctgaaacctc aggcatttga gaagcacacg gtcacactgc ttccggtagt
     8701 caataaaccg gtaaaccagc aatagacata agcggctatt taacgaccct gccctgaacc
     8761 gacgaccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt atcacttatt
     8821 caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg
     8881 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca
     8941 tcacaaacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta
     9001 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattggc cacgtttaaa
     9061 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac
     9121 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt
     9181 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc
     9241 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc
     9301 attgccatac ggaactccgg agcatctaac gcttgagtta agccgcgccg cgaagcggcg
     9361 tcggcttgaa cgaattgtta gacattattt gccgactacc ttggtgatct cgcctttcac
     9421 gtagtggaca aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc
     9481 aagataagcc tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat
     9541 tgcccagtcg gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat
     9601 gcgggacaac gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca
     9661 tagcgttaag gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag
     9721 ttcctccgcc gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat
     9781 agccagatca atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg
     9841 ccattctcca aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg
     9901 cacaacaatg gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt
     9961 ttccaaaagg tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt
    10021 aaccagcaaa tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa
    10081 atgtacggcc agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag
    10141 ttgagtcgat acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg
    10201 actgccctgc tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta
    10261 acgcgcttgc tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca
    10321 agccatgaaa accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca
    10381 gttgcgtgag cgcatacgct acttgcatta cagcttacga accgaacagg cttatgtcca
    10441 ctgggttcgt gccttcatcc gtttccacgg tgtgcgtcac ccggcaacct tgggcagcag
    10501 cgaagtcgag gcatttctgt cctggctggc gaacgagcgc aaggtttcgg tctccacgca
    10561 tcgtcaggca ttggcggcct tgctgttctt ctacggcaag gtgctgtgca cggatctgcc
    10621 ctggcttcag gagatcggaa gacctcggcc gtcgcggcgc ttgccggtgg tgctgacccc
    10681 ggatgaagtg gttcgcatcc tcggttttct ggaaggcgag catcgtttgt tcgcccagct
    10741 tctgtatgga acgggcatgc ggatcagtga gggtttgcaa ctgcgggtca aggatctgga
    10801 tttcgatcac ggcacgatca tcgtgcggga gggcaagggc tccaaggatc gggccttgat
    10861 gttacccgag agcttggcac ccagcctgcg cgagcagggg aattaattcc cacgggtttt
    10921 gctgcccgca aacgggctgt tctggtgttg ctagtttgtt atcagaatcg cagatccggc
    10981 ttcagccggt ttgccggctg aaagcgctat ttcttccaga attgccatga ttttttcccc
    11041 acgggaggcg tcactggctc ccgtgttgtc ggcagctttg attcgataag cagcatcgcc
    11101 tgtttcaggc tgtctatgtg tgactgttga gctgtaacaa gttgtctcag gtgttcaatt
    11161 tcatgttcta gttgctttgt tttactggtt tcacctgttc tattaggtgt tacatgctgt
    11221 tcatctgtta cattgtcgat ctgttcatgg tgaacagctt tgaatgcacc aaaaactcgt
    11281 aaaagctctg atgtatctat cttttttaca ccgttttcat ctgtgcatat ggacagtttt
    11341 ccctttgata tgtaacggtg aacagttgtt ctacttttgt ttgttagtct tgatgcttca
    11401 ctgatagata caagagccat aagaacctca gatccttccg tatttagcca gtatgttctc
    11461 tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa ccattgagat catacttact
    11521 ttgcatgtca ctcaaaa
//
