LOCUS       sequence-239010-        6557 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Knockdown for mouse Hmga2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Kishi Y, Fujii Y, Hirabayashi Y, Gotoh Y
  TITLE     HMGA regulates the global chromatin state and neurogenic potential 
            in neocortical precursor cells.
  JOURNAL   Nat Neurosci. 2012 Aug;15(8):1127-33. doi: 10.1038/nn.3165.
  PUBMED    22797695
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nn"; 
            journalName: "Nat Neurosci"; date: "2012-08"; volume: "15"; issue: 
            "8"; pages: "1127-33"
COMMENT     Sequence Label: pSiren_EGFP-shHmga2#3
FEATURES             Location/Qualifiers
     source          1..6557
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        63..562
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(98..121)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     495..514
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             join(583..585,586..588,589..1302)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(583..585,586..588,589..1299)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(628..649)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(889..908)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1236..1257
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             1424..1849
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(1952..1974)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2110..2130)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2115..2444
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2295..2430
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2466..2485
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2566..2583)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2737..3325)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2817..2836)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3496..4287,4288..4356))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4119..4138
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4357..4461)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4529..4547
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        4647..5026
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5027..5230
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5181..5201
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5231..5406
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    5469..5668
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5869..6285
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     6215..6237
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     6253..6269
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     promoter        6309..6557
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6309..6329
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6480..6499
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gcagatgggt cattgttgtg acgaatcaca acagtgagcc atctgctttt ttgaattcta
       61 ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct
      121 gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag
      181 gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt
      241 caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc
      301 acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct
      361 ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag
      421 gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc
      481 ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc
      541 ttcctcatct ccgggccttt cgacctgcag cccaagctta ccatggtgag caagggcgag
      601 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
      661 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
      721 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      781 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      841 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
      901 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      961 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     1021 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     1081 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1141 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     1201 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1261 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagctcgag atatcagtgg
     1321 tccaggctct agttttgact caacaatatc accagctgaa gcctatagag tacgagccat
     1381 agataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     1441 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac
     1501 tgagaataga gaagttcaga tcaaggtcag gaacagatgg aacagggtcg accctagaga
     1561 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     1621 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     1681 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac
     1741 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     1801 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
     1861 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggct
     1921 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     1981 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     2041 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtaga
     2101 tccggctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca
     2161 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct
     2221 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc
     2281 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     2341 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     2401 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt actggcttaa
     2461 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
     2521 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
     2581 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2641 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2701 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2761 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2821 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2881 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2941 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     3001 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3061 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3121 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     3181 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3241 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3301 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3361 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3421 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3481 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3541 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3601 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3661 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3721 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3781 taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt
     3841 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3901 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3961 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     4021 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     4081 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag
     4141 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     4201 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4261 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4321 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4381 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4441 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac
     4501 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca
     4561 agaattagct tggccattgc atacgttgta tccatatcat aatatgtaca tttatattgg
     4621 ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt aatagtaatc
     4681 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
     4741 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
     4801 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
     4861 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
     4921 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
     4981 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg
     5041 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc
     5101 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg
     5161 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat
     5221 aagcagagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ttccgataga
     5281 ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt
     5341 ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccacg acgggggtct
     5401 ttcatttggg ggctcgtccg ggatttggag acccctgccc agggaccacc gacccaccac
     5461 cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgttt
     5521 gatgttatgc gcctgcgtct gtactagtta gctaactagc tctgtatctg gcggacccgt
     5581 ggtggaactg acgagttctg aacacccggc cgcaaccctg ggagacgtcc cagggacttt
     5641 gggggccgtt tttgtggccc gacctgagga agggagtcga tgtggaatcc gaccccgtca
     5701 ggatatgtgg ttctggtagg agacgagaac ctaaaacagt tcccgcctcc gtctgaattt
     5761 ttgctttcgg tttggaaccg aagccgcgcg tcttgtctgc tgcagcgctg cagcatcgtt
     5821 ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg ccagactgtt
     5881 accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat cgctcacaac
     5941 cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga atggccaacc
     6001 tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac ccaggttaag
     6061 atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta catcgtgacc
     6121 tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca ccctaagcct
     6181 ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg ttcgaccccg
     6241 cctcgatcct ccctttatcc agccctcact ccttctctag gcgccggaat tgaagatctg
     6301 ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     6361 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     6421 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     6481 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     6541 ggaaaggacg aggatcc
//
