LOCUS       sequence-239155-       21007 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Abscisic acid-inducible B4GALT1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21007)
  AUTHORS   Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, 
            Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, 
            Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B 
            Jr, Summers NM, Lu TK, Weiss R
  TITLE     Small-molecule control of antibody N-glycosylation in engineered 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4.
  PUBMED    31110306
REFERENCE   2  (bases 1 to 21007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4."
COMMENT     Sequence Label: B4GALT1-ABA
FEATURES             Location/Qualifiers
     source          1..21007
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    26..46
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    64..101
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      145..154
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             151..549
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(551..575)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    730..785
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    1215..1240
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    1912..1932
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     2185..2209
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2226..2250
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3443..3467)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    3622..3677
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4038..4054)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4062..4078
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4086..4092,4093..4110,4111..4116))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4131..4152
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4276..4301
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    4973..4993
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5037..5248
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5249..6187
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    6212..6236
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6252..6272
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6273..6506
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     CDS             7047..7073
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(7084..7108)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7263..7318
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7679..7695)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7703..7719
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7727..7733,7734..7751,7752..7757))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7772..7793
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    7917..7942
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    8614..8634
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        8678..8889
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          8890..9828
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    9853..9877
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      9911..9920
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             11462..11485
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(11514..11538)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    11693..11748
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(12109..12125)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12133..12149
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12157..12163,12164..12181,12182..12187))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12202..12223
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12347..12372
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    13044..13064
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        13108..13319
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          13320..14258
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    14283..14307
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      14312..14321
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             14318..15031
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(15033..15057)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    15212..15267
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(15628..15644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    15652..15668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15676..15682,15683..15700,15701..15706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15721..15742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    16576..17412
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(17523..17542)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             17534..18343
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(17601..17620)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(18596..19184)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(18676..18695)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(19355..20146,20147..20215))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19978..19997
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(20216..20320)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(20346..20801)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(20483..20504)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     20695..20714
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     20928..20950
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     20942..20959
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     20943..20959
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20969..20988
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20969..20987
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgtcgacgt taacgctagt gatatcaact ttgtatagaa aagttggctc cgaatttctc
       61 gagggcttgt cgacgacggc ggtctccgtc gtcaggatca tgaattcgac ccaagtttgt
      121 acaaaaaagc aggctgaacg gcctgccacc atggccaagc ctttgtctca agaagaatcc
      181 accctcattg aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc
      241 gtcgccagcg cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat
      301 tttactgggg gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct
      361 ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc
      421 ggacggtgcc gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac
      481 agtgatggac agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg
      541 gagggctaat acccagcttt cttgtacaaa gtggtacgcg tgaattcact cctcaggtgc
      601 aggctgccta tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact
      661 gagatctttt tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga
      721 cttctggcta ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc
      781 tctcactcgg aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg
      841 tttagagttt ggcaacatat gcccatatgc tggctgccat gaacaaaggt tggctataaa
      901 gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat agaaaagcct
      961 tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct ttaacatccc
     1021 taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga ctactcccag
     1081 tcatagctgt ccctcttctc ttatggagat ccctcgacct gcagcccaag cttgaattct
     1141 ctagaattac cctgttatcc ctaggtttac cgagctctta ttggttttca aacttcattg
     1201 actgtgccaa gcttctgacc tcttctcttc ctcccacagg gcctcgagac aattgcatcg
     1261 atggtaccag tgaaagtctg caatgaattc gagttggcgc gcctgtcatt ctaaatctct
     1321 ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     1381 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     1441 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     1501 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggagggggc
     1561 tgtccccgtg agctcccagg cgcgcctgtc attctaaatc tctctttcag cctaaagctt
     1621 tttccccgta tccccccagg tgtctgcagg ctcaaagagc agcgagaagc gttcagagga
     1681 aagcgatccc gtgccacctt ccccgtgccc gggctgtccc cgcacgctgc cggctcgggg
     1741 atgcgggggg agcgccggac cggagcggag ccccgggcgg ctcgctgctg ccccctagcg
     1801 ggggagggac gtaattacat ccctgggggc tttggggggg ggctgtcccc gtgagctccc
     1861 aggcgcgcca actcggtacc gtatcgatgt cgacgttaac gctagtgata tcaactttgt
     1921 atagaaaagt tggctgcggc cgcgaaatga tacgaaacgt accgtatcgt taaggtgaat
     1981 gatacgaaac gtaccgtatc gttaaggtca atgatacgaa acgtaccgta tcgttaaggt
     2041 caatgatacg aaacgtaccg tatcgttaag gtgtatgata cgaaacgtac cgtatcgtta
     2101 aggtagatga tacgaaacgt accgtatcgt taaggtcggg tctcagctag ctaggcgtgt
     2161 acggtgggag gcctatataa gcagagctcg tttagtgaac cgtcagatcg ggtctcggaa
     2221 ttcgaccaag tttgtacaaa aaagcaggct gatgccacca tgaggtttct gaggccggtc
     2281 ctgggcggca gcgccgcgat gccgggcgcg accctgcagc gggcctgccg cctgctcgtg
     2341 gccgtctgcg cgctgcacct cggcgtcacc ctggtctatt acctctcggg ccgcgacctg
     2401 agccgcctgc ctcagctagt cggggtttcc tctacacttc ggagcggcac gatcggcgcc
     2461 accgccaaca agcagccccc cggcgcgcgg ccgccgcctc ctgtgggagt ctcctcgaag
     2521 cctcgcccgg gtcccgactc cagccctggt acggcttttg accccggcct gaagagcaac
     2581 tggacttcgg tcctagtgcc ccctaccact gcactgttga cgctgcccgc ttgccccgag
     2641 gagtccccgc tgctcgttgg ccccatggtg attgacttta atattgctgt ggacctggag
     2701 cttttggcaa agaagaaccc agagataaag atgggcggtc gttattcccc caaggactgt
     2761 atttctcctc acaaggtggc catcattatc ccatttcgca accggcagga gcacctcaaa
     2821 tattggctgt attatttgca tccggtccta cagcgccagc aactggacta tggtatctac
     2881 gtcatcaacc aggctggaga caccatgttc aatcgagcta agcttctcaa cataggcttc
     2941 caagaggcct tgaaggacca tgactacaac tgctttgtgt tcagtgatgt ggaccttatt
     3001 ccaatggacg accataatgc ctacaggtgt ttttcacagc cccggcacat ttctgttgca
     3061 atggacaagt ttgggtttag cctgccttat gttcagtact ttggaggcgt ttctgccctc
     3121 agtaaacaac agtttcttgc catcaatgga tttcctaata attattgggg ctggggagga
     3181 gaagatgatg acatttttaa cagaatagtt cataagggaa tgtctatatc acgcccaaat
     3241 gctgtagttg gaagatgtcg catgatacga cactcaagag acaagaaaaa tgagcccaat
     3301 cctcagaggt ttgaccggat tgctcacaca aaggaaacta tgcgctttga tggtttgaac
     3361 tcacttacct accaggtgtt gaacgtagag agatacccat tatataccaa aatcacagtg
     3421 gacatcggga caccaagata gtacccagct ttcttgtaca aagtggtacg cgtgaattca
     3481 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     3541 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3601 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3661 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3721 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3781 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3841 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3901 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3961 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     4021 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4081 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4141 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4201 cagcggatcg acagtactaa gcttggtgcg tttttatgct tgtagtattg tataatgttt
     4261 ttaagatcca agcttctgac ctcttctctt cctcccacag ggcctcgaga caattgcatc
     4321 gatggtacca gtgaaagtct gcaatgaatt cgagttggcg cgcctgtcat tctaaatctc
     4381 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     4441 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     4501 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     4561 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tgggaggggg
     4621 ctgtccccgt gagctcccag gcgcgcctgt cattctaaat ctctctttca gcctaaagct
     4681 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
     4741 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
     4801 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
     4861 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc cgtgagctcc
     4921 caggcgcgcc aactcggtac cgtatcgatg tcgacgttaa cgctagtgat atcaactttg
     4981 tatagaaaag ttggctccga attcgccctt attaattggc tccggtgccc gtcagtgggc
     5041 agagcgcaca tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaaccgg
     5101 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5161 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5221 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     5281 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     5341 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     5401 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     5461 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     5521 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     5581 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     5641 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     5701 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     5761 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     5821 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     5881 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     5941 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     6001 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     6061 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     6121 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     6181 atttcaggtg gatcccaagg gcgaattcga cccaagtttg tacaaaaaag caggcttagg
     6241 aattcatggg ccctaaaaag aagcgtaaag tcgccccccc gaccgatgtc agcctggggg
     6301 acgagctcca cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt
     6361 tcgatctgga catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact
     6421 ccgcccccta cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg
     6481 cccttggaat tgacgagtac ggtgggggcg cgccaactca agacgaattc acccaactct
     6541 cccaatcaat cgccgagttc cacacgtacc aactcggtaa cggccgttgc tcatctctcc
     6601 tagctcagcg aatccacgcg ccgccggaaa cagtatggtc cgtggtgaga cgtttcgata
     6661 ggccacagat ttacaaacac ttcatcaaaa gctgtaacgt gagtgaagat ttcgagatgc
     6721 gagtgggatg cacgcgcgac gtgaacgtga taagtggatt accggcgaat acgtctcgag
     6781 agagattaga tctgttggac gatgatcgga gagtgactgg gtttagtata accggtggtg
     6841 aacataggct gaggaattat aaatcggtta cgacggttca tagatttgag aaagaagaag
     6901 aagaagaaag gatctggacc gttgttttgg aatcttatgt tgttgatgta ccggaaggta
     6961 attcggagga agatacgaga ttgtttgctg atacggttat tagattgaat cttcagaaac
     7021 ttgcttcgat cactgaagct atgaactacc catacgatgt tccagattac gcttgaggat
     7081 cctacccagc tttcttgtac aaagtggtac gcgtgaattc actcctcagg tgcaggctgc
     7141 ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct
     7201 ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg
     7261 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     7321 cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag
     7381 tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca
     7441 tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg
     7501 aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt
     7561 ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc
     7621 tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt
     7681 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     7741 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     7801 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc gacagtacta
     7861 agcttggtct aatacccaat ctctcgtctt atccagatgt tttatacgcc aagcttctga
     7921 cctcttctct tcctcccaca gggcctcgag acaattgcat cgatggtacc agtgaaagtc
     7981 tgcaatgaat tcgagttggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt
     8041 ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa
     8101 agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga
     8161 tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg
     8221 gggagggacg taattacatc cctgggggct ttgggagggg gctgtccccg tgagctccca
     8281 ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca
     8341 ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc
     8401 ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg
     8461 accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac
     8521 atccctgggg gctttggggg ggggctgtcc ccgtgagctc ccaggcgcgc caactcggta
     8581 ccgtatcgat gtcgacgtta acgctagtga tatcaacttt gtatagaaaa gttggctccg
     8641 aattcgccct tattaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca
     8701 gtccccgaga agttgtgggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc
     8761 ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga
     8821 gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc
     8881 agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc
     8941 ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt
     9001 cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt
     9061 gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt
     9121 cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct
     9181 gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt
     9241 atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg
     9301 gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc
     9361 cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg
     9421 ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca
     9481 gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa
     9541 aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg
     9601 ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg
     9661 gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca
     9721 gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc
     9781 attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt ggatcccaag
     9841 ggcgaattcg acccaagttt gtacaaaaaa gcaggctgga tcccggtacc ctcgacacct
     9901 gctgacaggt gccaccatgg cccggacccc cagcagaagc agcatcggat ctctgagaag
     9961 cccccacacc cacaaggcca tcctgaccag caccatcgag atcctgaaag agtgcggcta
    10021 cagcggcctg agcatcgagt ctgtggctag aagggctggc gcctccaagc ccaccatcta
    10081 ccggtggtgg acaaacaagg ccgccctgat cgccgaggtg tacgagaacg agagcgagca
    10141 ggtccgaaag ttccccgacc tgggcagctt caaggccgat ctggacttcc tgctgcggaa
    10201 cctgtggaaa gtgtggcggg aaaccatctg cggcgaggcc ttcagatgcg tgatcgctga
    10261 ggcccagctg gaccctgcca cactgaccca gctgaaggac cagttcatgg aacggcggag
    10321 agagatgccc aagaaactgg tggaaaacgc catcagcaac ggcgagctgc ccaaggacac
    10381 caaccgggaa ctgctgctgg atatgatctt cggcttctgc tggtacagac tgctgaccga
    10441 gcagctgacc gtggaacagg atatcgagga attcaccttt ctgctgatca acggcgtgtg
    10501 ccccggcacc cagagagcgt tagcacttaa attagctggt ttggacatag gcggcgccag
    10561 cacgcgtgtg cctttgtatg gttttacttc gatttgtgga agaagacctg agatggaagc
    10621 tgctgtttcg actataccaa gattccttca atcttcctct ggttcgatgt tagatggtcg
    10681 gtttgatcct caatccgccg ctcatttctt cggtgtttac gacggccatg gcggttctca
    10741 ggtagcgaac tattgtagag agaggatgca tttggctttg gcggaggaga tagctaagga
    10801 gaaaccgatg ctctgcgatg gtgatacgtg gctggagaag tggaagaaag ctcttttcaa
    10861 ctcgttcctg agagttgact cggagattga gtcagttgcg ccggagacgg ttgggtcaac
    10921 gtcggtggtt gccgttgttt tcccgtctca catcttcgtc gctaactgcg gtgactctag
    10981 agccgttctt tgccgcggca aaactgcact tccattatcc gttgaccata aaccggatag
    11041 agaagatgaa gctgcgagga ttgaagccgc aggagggaaa gtgattcagt ggaatggagc
    11101 tcgtgttttc ggtgttctcg ccatgtcgag atccattggc gatagatact tgaaaccatc
    11161 catcattcct gatccggaag tgacggctgt gaagagagta aaagaagatg attgtctgat
    11221 tttggcgagt gacggggttt gggatgtaat gacggatgaa gaagcgtgtg agatggcaag
    11281 gaagcggatt ctcttgtggc acaagaaaaa cgcggtggct ggggatgcat cgttgctcgc
    11341 ggatgagcgg agaaaggaag ggaaagatcc tgcggcgatg tccgcggctg agtatttgtc
    11401 aaagctggcg atacagagag gaagcaaaga caacataagt gtggtggtgg ttgatttgaa
    11461 ggattacaag gacgatgacg ataagtgaag cttcagtgca ggtgaattct accacccagc
    11521 tttcttgtac aaagtggtac gcgtgaattc actcctcagg tgcaggctgc ctatcagaag
    11581 gtggtggctg gtgtggccaa tgccctggct cacaaatacc actgagatct ttttccctct
    11641 gccaaaaatt atggggacat catgaagccc cttgagcatc tgacttctgg ctaataaagg
    11701 aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca
    11761 tatgggaggg caaatcattt aaaacatcag aatgagtatt tggtttagag tttggcaaca
    11821 tatgcccata tgctggctgc catgaacaaa ggttggctat aaagaggtca tcagtatatg
    11881 aaacagcccc ctgctgtcca ttccttattc catagaaaag ccttgacttg aggttagatt
    11941 ttttttatat tttgttttgt gttatttttt tctttaacat ccctaaaatt ttccttacat
    12001 gttttactag ccagattttt cctcctctcc tgactactcc cagtcatagc tgtccctctt
    12061 ctcttatgga gatccctcga cctgcagccc aagcttggcg taatcatggt catagctgtt
    12121 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
    12181 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
    12241 gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc gacagtacta agcttggtga
    12301 attcccttat gtgagtgtaa aaggcaggcg agtttgtccc aagcttctga cctcttctct
    12361 tcctcccaca gggcctcgag acaattgcat cgatggtacc agtgaaagtc tgcaatgaat
    12421 tcgagttggc gcgcctgtca ttctaaatct ctctttcagc ctaaagcttt ttccccgtat
    12481 ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg ttcagaggaa agcgatcccg
    12541 tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc ggctcgggga tgcgggggga
    12601 gcgccggacc ggagcggagc cccgggcggc tcgctgctgc cccctagcgg gggagggacg
    12661 taattacatc cctgggggct ttgggagggg gctgtccccg tgagctccca ggcgcgcctg
    12721 tcattctaaa tctctctttc agcctaaagc tttttccccg tatcccccca ggtgtctgca
    12781 ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc ccgtgccacc ttccccgtgc
    12841 ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg ggagcgccgg accggagcgg
    12901 agccccgggc ggctcgctgc tgccccctag cgggggaggg acgtaattac atccctgggg
    12961 gctttggggg ggggctgtcc ccgtgagctc ccaggcgcgc caactcggta ccgtatcgat
    13021 gtcgacgtta acgctagtga tatcaacttt gtatagaaaa gttggctccg aattcgccct
    13081 tattaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
    13141 agttgtgggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
    13201 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
    13261 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
    13321 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
    13381 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
    13441 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
    13501 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
    13561 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
    13621 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
    13681 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
    13741 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
    13801 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
    13861 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
    13921 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
    13981 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
    14041 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
    14101 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
    14161 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
    14221 ctcagacagt ggttcaaagt ttttttcttc catttcaggt ggatcccaag ggcgaattcg
    14281 acccaagttt gtacaaaaaa gcaggctgat cgccaccatg gtgtctaagg gcgaagagct
    14341 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
    14401 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
    14461 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
    14521 cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
    14581 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
    14641 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
    14701 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
    14761 cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg ccctgaagct
    14821 cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc
    14881 taagaacctc aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga
    14941 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
    15001 ccctagcaaa ctggggcaca aacttaattg atacccagct ttcttgtaca aagtggtacg
    15061 cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
    15121 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
    15181 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
    15241 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
    15301 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
    15361 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
    15421 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
    15481 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
    15541 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
    15601 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    15661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    15721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    15781 cctgtcgtgc cagcggatcg acagtactaa gcttggttgc ttgcaaaagc agtaattgga
    15841 aagcactctc aaagaatccc aattgcatcg atggtaccag tgaaagtctg caatgaattc
    15901 gagttggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
    15961 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
    16021 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
    16081 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
    16141 attacatccc tgggggcttt gggagggggc tgtccccgtg agctcccagg cgcgcctgtc
    16201 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
    16261 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
    16321 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
    16381 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
    16441 tttggggggg ggctgtcccc gtgagctccc aggcgcgcca actcggtacc gtatcgatgt
    16501 cgacggtgcg tttttatgct tgtagtattg tataatgttt ttaagatcct agggataaca
    16561 gggtaatcgg ccgctactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc
    16621 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta
    16681 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt
    16741 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga
    16801 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga
    16861 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc
    16921 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg
    16981 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc
    17041 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga
    17101 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag
    17161 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca
    17221 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct
    17281 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc
    17341 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc
    17401 tggctctgct ctccaccgcg ggcagctctg gcccgtgtct caaaatctct gatgttacat
    17461 tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa
    17521 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
    17581 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
    17641 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
    17701 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
    17761 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
    17821 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
    17881 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
    17941 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
    18001 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
    18061 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
    18121 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
    18181 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
    18241 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
    18301 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
    18361 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
    18421 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gacggtatca ctagtattac
    18481 agaggtaagt tataacagtc gcctaaccca attgagggga taacgcagga aagaacatgt
    18541 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    18601 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    18661 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    18721 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    18781 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    18841 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    18901 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    18961 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    19021 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    19081 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    19141 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    19201 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    19261 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    19321 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    19381 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    19441 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    19501 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    19561 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    19621 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    19681 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    19741 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    19801 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    19861 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    19921 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    19981 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    20041 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    20101 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    20161 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    20221 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    20281 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    20341 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
    20401 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
    20461 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
    20521 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
    20581 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
    20641 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
    20701 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
    20761 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
    20821 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    20881 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    20941 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
    21001 cgtatcg
//
