LOCUS       sequence-239166-        9489 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-KanMx integrating (YIp) vector for stable genomic integration 
            in the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-KanMx
FEATURES             Location/Qualifiers
     source          1..9489
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        89..328
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             329..4507
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     primer_bind     complement(4908..4927)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5027..5049
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5087..5105)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            complement(5158..6514)
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     terminator      complement(5158..5355)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(5361..6170)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6084..6103
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(6171..6514)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        6587..6691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6692..6760,6761..7552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6910..6929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7723..8311
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8212..8231
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8465..8482
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8599..8620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8635..8640,8641..8658,8659..8665)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8673..8689
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8678..8700
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8697..8713
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8697..8713
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             8709..9089
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     8732..8752
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8734..8752
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    8765..8872
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     8789..8805
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8789..8805
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8839..8855)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8840..8856)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(8880..8899)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8881..8899)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8909..8926)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8909..8925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8918..8940)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(9066..9489,1..32)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9153..9172)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9363..9384
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gaattttaac aaaatattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
       61 ctgtgcggta tttcacaccg catagatcaa ttccacttgc aattacataa aaaattccgg
      121 cggtttttcg cgtgtgactc aatgtcgaaa tacctgccta atgaacatga acatcgccca
      181 aatgtatttg aagacccgct gggagaagtt caagatatat aagtaacaag cagccaatag
      241 tataaaaaaa aatctgagtt tattaccttt cctggaattt cagtgaaaaa ctgctaatta
      301 tagagagata tcacagagtt actcactaat gactaacgaa aaggtctgga tagagaagtt
      361 ggataatcca actctttcag tgttaccaca tgacttttta cgcccacaac aagaacctta
      421 tacgaaacaa gctacatatt cgttacagct acctcagctc gatgtgcctc atgatagttt
      481 ttctaacaaa tacgctgtcg ctttgagtgt atgggctgca ttgatatata gagtaaccgg
      541 tgacgatgat attgttcttt atattgcgaa taacaaaatc ttaagattca atattcaacc
      601 aacgtggtca tttaatgagc tgtattctac aattaacaat gagttgaaca agctcaattc
      661 tattgaggcc aatttttcct ttgacgagct agctgaaaaa attcaaagtt gccaagatct
      721 ggaaaggacc cctcagttgt tccgtttggc ctttttggaa aaccaagatt tcaaattaga
      781 cgagttcaag catcatttag tggactttgc tttgaatttg gataccagta ataatgcgca
      841 tgttttgaac ttaatttata acagcttact gtattcgaat gaaagagtaa ccattgttgc
      901 ggaccaattt actcaatatt tgactgctgc gctaagcgat ccatccaatt gcataactaa
      961 aatctctctg atcaccgcat catccaagga tagtttacct gatccaacta agaacttggg
     1021 ctggtgcgat ttcgtggggt gtattcacga cattttccag gacaatgctg aagccttccc
     1081 agagagaacc tgtgttgtgg agactccaac actaaattcc gacaagtccc gttctttcac
     1141 ttatcgcgac atcaaccgca cttctaacat agttgcccat tatttgatta aaacaggtat
     1201 caaaagaggt gatgtagtga tgatctattc ttctaggggt gtggatttga tggtatgtgt
     1261 gatgggtgtc ttgaaagccg gcgcaacctt ttcagttatc gaccctgcat atcccccagc
     1321 cagacaaacc atttacttag gtgttgctaa accacgtggg ttgattgtta ttagagctgc
     1381 tggacaattg gatcaactag tagaagatta catcaatgat gaattggaga ttgtttcaag
     1441 aatcaattcc atcgctattc aagaaaatgg taccattgaa ggtggcaaat tggacaatgg
     1501 cgaggatgtt ttggctccat atgatcacta caaagacacc agaacaggtg ttgtagttgg
     1561 accagattcc aacccaaccc tatctttcac atctggttcc gaaggtattc ctaagggtgt
     1621 tcttggtaga catttttcct tggcttatta tttcaattgg atgtccaaaa ggttcaactt
     1681 aacagaaaat gataaattca caatgctgag cggtattgca catgatccaa ttcaaagaga
     1741 tatgtttaca ccattatttt taggtgccca attgtatgtc cctactcaag atgatattgg
     1801 tacaccgggc cgtttagcgg aatggatgag taagtatggt tgcacagtta cccatttaac
     1861 acctgccatg ggtcaattac ttactgccca agctactaca ccattcccta agttacatca
     1921 tgcgttcttt gtgggtgaca ttttaacaaa acgtgattgt ctgaggttac aaaccttggc
     1981 agaaaattgc cgtattgtta atatgtacgg taccactgaa acacagcgtg cagtttctta
     2041 tttcgaagtt aaatcaaaaa atgacgatcc aaactttttg aaaaaattga aagatgtcat
     2101 gcctgctggt aaaggtatgt tgaacgttca gctactagtt gttaacagga acgatcgtac
     2161 tcaaatatgt ggtattggcg aaataggtga gatttatgtt cgtgcaggtg gtttggccga
     2221 aggttataga ggattaccag aattgaataa agaaaaattt gtgaacaact ggtttgttga
     2281 aaaagatcac tggaattatt tggataagga taatggtgaa ccttggagac aattctggtt
     2341 aggtccaaga gatagattgt acagaacggg tgatttaggt cgttatctac caaacggtga
     2401 ctgtgaatgt tgcggtaggg ctgatgatca agttaaaatt cgtgggttca gaatcgaatt
     2461 aggagaaata gatacgcaca tttcccaaca tccattggta agagaaaaca ttactttagt
     2521 tcgcaaaaat gccgacaatg agccaacatt gatcacattt atggtcccaa gatttgacaa
     2581 gccagatgac ttgtctaagt tccaaagtga tgttccaaag gaggttgaaa ctgaccctat
     2641 agttaagggc ttaatcggtt accatctttt atccaaggac atcaggactt tcttaaagaa
     2701 aagattggct agctatgcta tgccttcctt gattgtggtt atggataaac taccattgaa
     2761 tccaaatggt aaagttgata agcctaaact tcaattccca actcccaagc aattaaattt
     2821 ggtagctgaa aatacagttt ctgaaactga cgactctcag tttaccaatg ttgagcgcga
     2881 ggttagagac ttatggttaa gtatattacc taccaagcca gcatctgtat caccagatga
     2941 ttcgtttttc gatttaggtg gtcattctat cttggctacc aaaatgattt ttaccttaaa
     3001 gaaaaagctg caagttgatt taccattggg cacaattttc aagtatccaa cgataaaggc
     3061 ctttgccgcg gaaattgaca gaattaaatc atcgggtgga tcatctcaag gtgaggtcgt
     3121 cgaaaatgtc actgcaaatt atgcggaaga cgccaagaaa ttggttgaga cgctaccaag
     3181 ttcgtacccc tctcgagaat attttgttga acctaatagt gccgaaggaa aaacaacaat
     3241 taatgtgttt gttaccggtg tcacaggatt tctgggctcc tacatccttg cagatttgtt
     3301 aggacgttct ccaaagaact acagtttcaa agtgtttgcc cacgtcaggg ccaaggatga
     3361 agaagctgca tttgcaagat tacaaaaggc aggtatcacc tatggtactt ggaacgaaaa
     3421 atttgcctca aatattaaag ttgtattagg cgatttatct aaaagccaat ttggtctttc
     3481 agatgagaag tggatggatt tggcaaacac agttgatata attatccata atggtgcgtt
     3541 agttcactgg gtttatccat atgccaaatt gagggatcca aatgttattt caactatcaa
     3601 tgttatgagc ttagccgccg tcggcaagcc aaagttcttt gactttgttt cctccacttc
     3661 tactcttgac actgaatact actttaattt gtcagataaa cttgttagcg aagggaagcc
     3721 aggcatttta gaatcagacg atttaatgaa ctctgcaagc gggctcactg gtggatatgg
     3781 tcagtccaaa tgggctgctg agtacatcat tagacgtgca ggtgaaaggg gcctacgtgg
     3841 gtgtattgtc agaccaggtt acgtaacagg tgcctctgcc aatggttctt caaacacaga
     3901 tgatttctta ttgagatttt tgaaaggttc agtccaatta ggtaagattc cagatatcga
     3961 aaattccgtg aatatggttc cagtagatca tgttgctcgt gttgttgttg ctacgtcttt
     4021 gaatcctccc aaagaaaatg aattggccgt tgctcaagta acgggtcacc caagaatatt
     4081 attcaaagac tacttgtata ctttacacga ttatggttac gatgtcgaaa tcgaaagcta
     4141 ttctaaatgg aagaaatcat tggaggcgtc tgttattgac aggaatgaag aaaatgcgtt
     4201 gtatcctttg ctacacatgg tcttagacaa cttacctgaa agtaccaaag ctccggaact
     4261 agacgatagg aacgccgtgg catctttaaa gaaagacacc gcatggacag gtgttgattg
     4321 gtctaatgga ataggtgtta ctccagaaga ggttggtata tatattgcat ttttaaacaa
     4381 ggttggattt ttacctccac caactcataa tgacaaactt ccactgccaa gtatagaact
     4441 aactcaagcg caaataagtc tagttgcttc aggtgctggt gctcgtggaa gctccgcagc
     4501 agcttaaggt tgagcattac gtatgatatg tccatgtaca ataattaaat atgaattagg
     4561 agaaagactt agcttctttt cgggtgatgt cacttaaaaa ctccgagaat aatatataat
     4621 aagagaataa aatattagtt attgaataag aactgtaaat cagctggcgt tagtctgcta
     4681 atggcagctt catcttggtt tattgtagca tgaatcatat ttgccttttt ttcctgtaat
     4741 tcaatgattc ttgcttctat actatcctca atgcaaaacc ttgtgatctt cacaggtcga
     4801 tactgaccaa ttctatgaac tctatcacca ctttgccatt caacactagg gttccaccat
     4861 gggtctaaaa tgaatacttg cgaagctgat ctatggtgca ctctcagtac aatctgctct
     4921 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4981 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5041 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5101 ctatttttat aggttaatgt catgatctgg atggcggcgt tagtatcgaa tcgacagcag
     5161 tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg cacttaactt
     5221 cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa catttgatta
     5281 aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga aaacaagaat
     5341 ctttttattg tcagtactga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
     5401 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
     5461 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
     5521 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
     5581 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
     5641 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
     5701 ccgttattca ttcgtgattg cgcctgagcg agacgaaata cgcgatcgct gttaaaagga
     5761 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
     5821 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttgcc ggggatcgca
     5881 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
     5941 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
     6001 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
     6061 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
     6121 atgttggaat ttaatcgcgg cctcgaaacg tgagtctttt ccttacccat ggttgtttat
     6181 gttcggatgt gatgtgagaa ctgtatccta gcaagatttt aaaaggaagt atatgaaaga
     6241 agaacctcag tggcaaatcc taacctttta tatttctcta caggggcgcg gcgtggggac
     6301 aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt
     6361 aatttttgct tcgcgccgtg cggccatcaa aatgtatgga tgcaaatgat tatacatggg
     6421 gatgtatggg ctaaatgtac gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa
     6481 gactgtcaag gagggtattc tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg
     6541 aggcaagcta aacaagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     6601 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     6661 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     6721 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     6781 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     6841 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     6901 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     6961 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7021 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7081 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7141 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7201 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     7261 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     7321 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     7381 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     7441 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     7501 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     7561 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     7621 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     7681 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     7741 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     7801 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     7861 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     7921 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     7981 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8041 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8101 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8161 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     8221 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     8281 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     8341 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     8401 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     8461 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     8521 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     8581 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     8641 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     8701 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     8761 gctggagctc caccgcggtg gcggccgctc tagaactagt ggatcccccg ggctgcagga
     8821 attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta cccaattcgc
     8881 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     8941 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     9001 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     9061 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     9121 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     9181 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     9241 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     9301 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     9361 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     9421 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9481 atttaacgc
//
