LOCUS       sequence-239167-        7871 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ADE2-HphMx ICE plasmid used to facilitate yeast recombination 
            cloning and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 7871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE ADE2-HphMx
FEATURES             Location/Qualifiers
     source          1..7871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..431
                     /gene="S. cerevisiae ADE2"
                     /label=ADE2 promoter
     CDS             432..2147
                     /codon_start=1
                     /gene="S. cerevisiae ADE2"
                     /product="phosphoribosylaminoimidazole carboxylase, 
                     required for adenine biosynthesis"
                     /label=ADE2
                     /note="yeast auxotrophic marker"
                     /translation="MDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     rep_origin      complement(2352..2807)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2489..2510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2701..2720
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2933..2955
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2947..2964
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2948..2964
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2974..2993
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2974..2992
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3017..3033
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3018..3034
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3068..3084)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3068..3084)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    3115..3618
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(3673..3693)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3673..3691)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3712..3728)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3712..3728)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3725..3747)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3736..3752
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3760..3766,3767..3784,3785..3790))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3805..3826
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3943..3960)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4114..4702)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4194..4213)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4873..5664,5665..5733))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5496..5515
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5734..5838)
                     /gene="bla"
                     /label=AmpR promoter
     gene            5911..7486
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        5911..6254
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6255..7283
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             6255..7283
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      7289..7486
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     7539..7557
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7595..7617)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7717..7736
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aataggccga aatcggcaaa atcccttata aatcaaaaga atagatctga attaattctt
       61 gaataataca taacttttct taaaagaatc aaagacagat aaaatttaag agatattaaa
      121 tattagtgag aagccgagaa ttttgtaaca ccaacataac actgacatct ttaacaactt
      181 ttaattatga tacatttctt acgtcatgat tgattattac agctatgctg acaaatgact
      241 cttgttgcat ggctacgaac cgggtaatac taagtgattg actcttgctg accttttatt
      301 aagaactaaa tggacaatat tatggagcat ttcatgtata aattggtgcg taaaatcgtt
      361 ggatctctct tctaagtaca tcctactata acaatcaaga aaaacaagaa aatcggacaa
      421 aacaatcaag tatggattct agaacagttg gtatattagg agggggacaa ttgggacgta
      481 tgattgttga ggcagcaaac aggctcaaca ttaagacggt aatactagat gctgaaaatt
      541 ctcctgccaa acaaataagc aactccaatg accacgttaa tggctccttt tccaatcctc
      601 ttgatatcga aaaactagct gaaaaatgtg atgtgctaac gattgagatt gagcatgttg
      661 atgttcctac actaaagaat cttcaagtaa aacatcccaa attaaaaatt tacccttctc
      721 cagaaacaat cagattgata caagacaaat atattcaaaa agagcattta atcaaaaatg
      781 gtatagcagt tacccaaagt gttcctgtgg aacaagccag tgagacgtcc ctattgaatg
      841 ttggaagaga tttgggtttt ccattcgtct tgaagtcgag gactttggca tacgatggaa
      901 gaggtaactt cgttgtaaag aataaggaaa tgattccgga agctttggaa gtactgaagg
      961 atcgtccttt gtacgccgaa aaatgggcac catttactaa agaattagca gtcatgattg
     1021 tgaggtctgt taacggttta gtgttttctt acccaattgt agagactatc cacaaggaca
     1081 atatttgtga cttatgttat gcgcctgcta gagttccgga ctccgttcaa cttaaggcga
     1141 agttgttggc agaaaatgca atcaaatctt ttcccggttg tggtatattt ggtgtggaaa
     1201 tgttctattt agaaacaggg gaattgctta ttaacgaaat tgccccaagg cctcacaact
     1261 ctggacatta taccattgat gcttgcgtca cttctcaatt tgaagctcat ttgagatcaa
     1321 tattggattt gccaatgcca aagaatttca catctttctc caccattaca acgaacgcca
     1381 ttatgctaaa tgttcttgga gacaaacata caaaagataa agagctagaa acttgcgaaa
     1441 gagcattggc gactccaggt tcctcagtgt acttatatgg aaaagagtct agacctaaca
     1501 gaaaagtagg tcacataaat attattgcct ccagtatggc ggaatgtgaa caaaggctga
     1561 actacattac aggtagaact gatattccaa tcaaaatctc tgtcgctcaa aagttggact
     1621 tggaagcaat ggtcaaacca ttggttggaa tcatcatggg atcagactct gacttgccgg
     1681 taatgtctgc cgcatgtgcg gttttaaaag attttggcgt tccatttgaa gtgacaatag
     1741 tctctgctca tagaactcca cataggatgt cagcatatgc tatttccgca agcaagcgtg
     1801 gaattaaaac aattatcgct ggagctggtg gggctgctca cttgccaggt atggtggctg
     1861 caatgacacc acttcctgtc atcggtgtgc ccgtaaaagg ttcttgtcta gatggagtag
     1921 attctttaca ttcaattgtg caaatgccta gaggtgttcc agtagctacc gtcgctatta
     1981 ataatagtac gaacgctgcg ctgttggctg tcagactgct tggcgcttat gattcaagtt
     2041 atacaacgaa aatggaacag tttttattaa agcaagaaga agaagttctt gtcaaagcac
     2101 aaaagttaga aactgtcggt tacgaagctt atctagaaaa caagtaatat ataagtttat
     2161 tgatatactt gtacagcaaa taattataaa atgatatacc tattttttag gctttgttat
     2221 gattacatca aatgtggact tcatacatag aaatcaacgc ttacaggtgt ccttttttaa
     2281 gaatttcata cataagatct atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     2341 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     2401 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     2461 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     2521 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     2581 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     2641 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     2701 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     2761 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     2821 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     2881 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     2941 cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg actcactata gggcgaattg
     3001 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga attcctgcag
     3061 cccgggggat ccactagttc tagagcggcc gccctcttag cgcttcttag gacggatcgc
     3121 ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg ggaatttact
     3181 ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata aagaaggtag
     3241 aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc aacaaaaagc
     3301 gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat acattcgatt
     3361 aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac acagacaaga
     3421 tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg tagtatttgt
     3481 tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt ttctttaatt
     3541 tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta aattataatt
     3601 atttttatag cacgtgatga aaaggaccca ggtggcgcgg ccgccaccgc ggtggagctc
     3661 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     3721 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3781 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3841 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3901 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3961 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     4021 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     4081 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     4141 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     4201 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     4261 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     4321 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4381 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4441 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4501 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4561 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4621 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4681 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4741 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4801 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4861 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4921 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4981 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     5041 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     5101 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     5161 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     5221 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     5281 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     5341 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5401 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5461 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5521 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5581 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5641 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5701 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5761 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5821 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct tgtttagctt
     5881 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
     5941 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
     6001 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
     6061 aattacggct cctcgctgcg gacctgcgag cagggaaacg ctcccctcac agacgcgttg
     6121 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gttaggattt gccactgagg
     6181 ttcttctttc atatacttcc ttttaaaatc ttgctaggat acagttctca catcacatcc
     6241 gaacataaac aaccatgggt aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc
     6301 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc
     6361 gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg
     6421 atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc
     6481 cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg
     6541 cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg
     6601 tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc
     6661 cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg
     6721 ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg
     6781 cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg
     6841 tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca
     6901 ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct
     6961 ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg
     7021 agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct
     7081 atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg
     7141 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
     7201 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
     7261 ctcgtccgag ggcaaaggaa taatcagtac tgacaataaa aagattcttg ttttcaagaa
     7321 cttgtcattt gtatagtttt tttatattgt agttgttcta ttttaatcaa atgttagcgt
     7381 gatttatatt ttttttcgcc tcgacatcat ctgcccagat gcgaagttaa gtgcgcagaa
     7441 agtaatatca tgcgtcaatc gtatgtgaat gctggtcgct atactgctgt cgattcgata
     7501 ctaacgccgc catccagatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     7561 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     7621 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     7681 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     7741 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     7801 ggaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     7861 attttttaac c
//
