LOCUS       sequence-239168-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-HphMx ICE plasmid used to facilitate yeast recombination 
            cloning and stable genomic Integration after CEN Excision..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: pICE LYS2-HphMx
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(334..4512)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(4513..4752)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     rep_origin      complement(4809..5264)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4946..4967)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5158..5177
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5390..5412
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5404..5421
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5405..5421
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5431..5450
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5431..5449
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     5474..5490
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5475..5491
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5525..5541)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5525..5541)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    5572..6075
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     complement(6130..6150)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(6130..6148)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6169..6185)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6169..6185)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6182..6204)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6193..6209
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6217..6223,6224..6241,6242..6247))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6262..6283
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6400..6417)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6571..7159)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6651..6670)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7330..8121,8122..8190))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7953..7972
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8191..8295)
                     /gene="bla"
                     /label=AmpR promoter
     gene            8368..9943
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     promoter        8368..8711
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             8712..9740
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             8712..9740
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     terminator      9746..9943
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     9996..10014
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10052..10074)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10174..10193
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 aatggcaaag tggtgataga gttcatagaa ttggtcagta tcgacctgtg aagatcacaa
       61 ggttttgcat tgaggatagt atagaagcaa gaatcattga attacaggaa aaaaaggcaa
      121 atatgattca tgctacaata aaccaagatg aagctgccat tagcagacta acgccagctg
      181 atttacagtt cttattcaat aactaatatt ttattctctt attatatatt attctcggag
      241 tttttaagtg acatcacccg aaaagaagct aagtctttct cctaattcat atttaattat
      301 tgtacatgga catatcatac gtaatgctca accttaagct gctgcggagc ttccacgagc
      361 accagcacct gaagcaacta gacttatttg cgcttgagtt agttctatac ttggcagtgg
      421 aagtttgtca ttatgagttg gtggaggtaa aaatccaacc ttgtttaaaa atgcaatata
      481 tataccaacc tcttctggag taacacctat tccattagac caatcaacac ctgtccatgc
      541 ggtgtctttc tttaaagatg ccacggcgtt cctatcgtct agttccggag ctttggtact
      601 ttcaggtaag ttgtctaaga ccatgtgtag caaaggatac aacgcatttt cttcattcct
      661 gtcaataaca gacgcctcca atgatttctt ccatttagaa tagctttcga tttcgacatc
      721 gtaaccataa tcgtgtaaag tatacaagta gtctttgaat aatattcttg ggtgacccgt
      781 tacttgagca acggccaatt cattttcttt gggaggattc aaagacgtag caacaacaac
      841 acgagcaaca tgatctactg gaaccatatt cacggaattt tcgatatctg gaatcttacc
      901 taattggact gaacctttca aaaatctcaa taagaaatca tctgtgtttg aagaaccatt
      961 ggcagaggca cctgttacgt aacctggtct gacaatacac ccacgtaggc ccctttcacc
     1021 tgcacgtcta atgatgtact cagcagccca tttggactga ccatatccac cagtgagccc
     1081 gcttgcagag ttcattaaat cgtctgattc taaaatgcct ggcttccctt cgctaacaag
     1141 tttatctgac aaattaaagt agtattcagt gtcaagagta gaagtggagg aaacaaagtc
     1201 aaagaacttt ggcttgccga cggcggctaa gctcataaca ttgatagttg aaataacatt
     1261 tggatccctc aatttggcat atggataaac ccagtgaact aacgcaccat tatggataat
     1321 tatatcaact gtgtttgcca aatccatcca cttctcatct gaaagaccaa attggctttt
     1381 agataaatcg cctaatacaa ctttaatatt tgaggcaaat ttttcgttcc aagtaccata
     1441 ggtgatacct gccttttgta atcttgcaaa tgcagcttct tcatccttgg ccctgacgtg
     1501 ggcaaacact ttgaaactgt agttctttgg agaacgtcct aacaaatctg caaggatgta
     1561 ggagcccaga aatcctgtga caccggtaac aaacacatta attgttgttt ttccttcggc
     1621 actattaggt tcaacaaaat attctcgaga ggggtacgaa cttggtagcg tctcaaccaa
     1681 tttcttggcg tcttccgcat aatttgcagt gacattttcg acgacctcac cttgagatga
     1741 tccacccgat gatttaattc tgtcaatttc cgcggcaaag gcctttatcg ttggatactt
     1801 gaaaattgtg cccaatggta aatcaacttg cagctttttc tttaaggtaa aaatcatttt
     1861 ggtagccaag atagaatgac cacctaaatc gaaaaacgaa tcatctggtg atacagatgc
     1921 tggcttggta ggtaatatac ttaaccataa gtctctaacc tcgcgctcaa cattggtaaa
     1981 ctgagagtcg tcagtttcag aaactgtatt ttcagctacc aaatttaatt gcttgggagt
     2041 tgggaattga agtttaggct tatcaacttt accatttgga ttcaatggta gtttatccat
     2101 aaccacaatc aaggaaggca tagcatagct agccaatctt ttctttaaga aagtcctgat
     2161 gtccttggat aaaagatggt aaccgattaa gcccttaact atagggtcag tttcaacctc
     2221 ctttggaaca tcactttgga acttagacaa gtcatctggc ttgtcaaatc ttgggaccat
     2281 aaatgtgatc aatgttggct cattgtcggc atttttgcga actaaagtaa tgttttctct
     2341 taccaatgga tgttgggaaa tgtgcgtatc tatttctcct aattcgattc tgaacccacg
     2401 aattttaact tgatcatcag ccctaccgca acattcacag tcaccgtttg gtagataacg
     2461 acctaaatca cccgttctgt acaatctatc tcttggacct aaccagaatt gtctccaagg
     2521 ttcaccatta tccttatcca aataattcca gtgatctttt tcaacaaacc agttgttcac
     2581 aaatttttct ttattcaatt ctggtaatcc tctataacct tcggccaaac cacctgcacg
     2641 aacataaatc tcacctattt cgccaatacc acatatttga gtacgatcgt tcctgttaac
     2701 aactagtagc tgaacgttca acataccttt accagcaggc atgacatctt tcaatttttt
     2761 caaaaagttt ggatcgtcat tttttgattt aacttcgaaa taagaaactg cacgctgtgt
     2821 ttcagtggta ccgtacatat taacaatacg gcaattttct gccaaggttt gtaacctcag
     2881 acaatcacgt tttgttaaaa tgtcacccac aaagaacgca tgatgtaact tagggaatgg
     2941 tgtagtagct tgggcagtaa gtaattgacc catggcaggt gttaaatggg taactgtgca
     3001 accatactta ctcatccatt ccgctaaacg gcccggtgta ccaatatcat cttgagtagg
     3061 gacatacaat tgggcaccta aaaataatgg tgtaaacata tctctttgaa ttggatcatg
     3121 tgcaataccg ctcagcattg tgaatttatc attttctgtt aagttgaacc ttttggacat
     3181 ccaattgaaa taataagcca aggaaaaatg tctaccaaga acacccttag gaataccttc
     3241 ggaaccagat gtgaaagata gggttgggtt ggaatctggt ccaactacaa cacctgttct
     3301 ggtgtctttg tagtgatcat atggagccaa aacatcctcg ccattgtcca atttgccacc
     3361 ttcaatggta ccattttctt gaatagcgat ggaattgatt cttgaaacaa tctccaattc
     3421 atcattgatg taatcttcta ctagttgatc caattgtcca gcagctctaa taacaatcaa
     3481 cccacgtggt ttagcaacac ctaagtaaat ggtttgtctg gctgggggat atgcagggtc
     3541 gataactgaa aaggttgcgc cggctttcaa gacacccatc acacatacca tcaaatccac
     3601 acccctagaa gaatagatca tcactacatc acctcttttg atacctgttt taatcaaata
     3661 atgggcaact atgttagaag tgcggttgat gtcgcgataa gtgaaagaac gggacttgtc
     3721 ggaatttagt gttggagtct ccacaacaca ggttctctct gggaaggctt cagcattgtc
     3781 ctggaaaatg tcgtgaatac accccacgaa atcgcaccag cccaagttct tagttggatc
     3841 aggtaaacta tccttggatg atgcggtgat cagagagatt ttagttatgc aattggatgg
     3901 atcgcttagc gcagcagtca aatattgagt aaattggtcc gcaacaatgg ttactctttc
     3961 attcgaatac agtaagctgt tataaattaa gttcaaaaca tgcgcattat tactggtatc
     4021 caaattcaaa gcaaagtcca ctaaatgatg cttgaactcg tctaatttga aatcttggtt
     4081 ttccaaaaag gccaaacgga acaactgagg ggtcctttcc agatcttggc aactttgaat
     4141 tttttcagct agctcgtcaa aggaaaaatt ggcctcaata gaattgagct tgttcaactc
     4201 attgttaatt gtagaataca gctcattaaa tgaccacgtt ggttgaatat tgaatcttaa
     4261 gattttgtta ttcgcaatat aaagaacaat atcatcgtca ccggttactc tatatatcaa
     4321 tgcagcccat acactcaaag cgacagcgta tttgttagaa aaactatcat gaggcacatc
     4381 gagctgaggt agctgtaacg aatatgtagc ttgtttcgta taaggttctt gttgtgggcg
     4441 taaaaagtca tgtggtaaca ctgaaagagt tggattatcc aacttctcta tccagacctt
     4501 ttcgttagtc attagtgagt aactctgtga tatctctcta taattagcag tttttcactg
     4561 aaattccagg aaaggtaata aactcagatt tttttttata ctattggctg cttgttactt
     4621 atatatcttg aacttctccc agcgggtctt caaatacatt tgggcgatgt tcatgttcat
     4681 taggcaggta tttcgacatt gagtcacacg cgaaaaaccg ccggaatttt ttatgtaatt
     4741 gcaagtggaa ttgatctatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     4801 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     4861 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     4921 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     4981 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     5041 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     5101 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     5161 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     5221 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     5281 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     5341 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     5401 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt
     5461 accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc
     5521 gggggatcca ctagttctag agcggccgcc ctcttagcgc ttcttaggac ggatcgcttg
     5581 cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga atttactctg
     5641 tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag aaggtagaag
     5701 agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac aaaaagcgta
     5761 ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca ttcgattaac
     5821 gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca gacaagatga
     5881 aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag tatttgttgg
     5941 cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc tttaatttct
     6001 ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat tataattatt
     6061 tttatagcac gtgatgaaaa ggacccaggt ggcgcggccg ccaccgcggt ggagctccag
     6121 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     6181 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     6241 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     6301 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     6361 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6421 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6481 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6541 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6601 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6661 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6721 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6781 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6841 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6901 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6961 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     7021 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     7081 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7141 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7201 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7261 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7321 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7381 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7441 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7501 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7561 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7621 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7681 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7741 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7801 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7861 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7921 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7981 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     8041 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     8101 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     8161 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8221 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8281 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtcttgt ttagcttgcc
     8341 tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt
     8401 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
     8461 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
     8521 tacggctcct cgctgcggac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
     8581 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
     8641 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
     8701 cataaacaac catgggtaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
     8761 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
     8821 ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
     8881 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
     8941 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
     9001 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
     9061 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
     9121 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
     9181 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
     9241 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
     9301 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
     9361 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
     9421 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
     9481 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
     9541 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
     9601 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
     9661 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
     9721 gtccgagggc aaaggaataa tcagtactga caataaaaag attcttgttt tcaagaactt
     9781 gtcatttgta tagttttttt atattgtagt tgttctattt taatcaaatg ttagcgtgat
     9841 ttatattttt tttcgcctcg acatcatctg cccagatgcg aagttaagtg cgcagaaagt
     9901 aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata ctgctgtcga ttcgatacta
     9961 acgccgccat ccagatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
    10021 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
    10081 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
    10141 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
    10201 tgcaccatag atcagcttcg caagtattca ttttagaccc atggtggaac cctagtgttg
//
