LOCUS       sequence-239180-        9708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-HphMx integrating (YIp) vector for stable genomic integration 
            in the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-HphMx
FEATURES             Location/Qualifiers
     source          1..9708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..77)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     177..199
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(237..255)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            complement(308..1883)
                     /label=hphMX6
                     /note="yeast selectable marker conferring hygromycin 
                     resistance"
     terminator      complement(308..505)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(511..1539)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     CDS             complement(511..1539)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MGKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
                     GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDL
                     PETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHW
                     QTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAM
                     FGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVD
                     GNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRA
                     KE"
     promoter        complement(1540..1883)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        1956..2060
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2061..2129,2130..2921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2279..2298)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3092..3680
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3581..3600
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3834..3851
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3968..3989
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4004..4009,4010..4027,4028..4034)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4042..4058
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4047..4069
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4066..4082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4066..4082
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             4078..4458
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     4101..4121
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4103..4121
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    4134..4241
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     4158..4174
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4158..4174
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4208..4224)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4209..4225)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4249..4268)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4250..4268)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4278..4295)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4278..4294)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4287..4309)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4435..4890
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4522..4541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4732..4753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4947..5186
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             5187..9365
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
ORIGIN
        1 gttccaccat gggtctaaaa tgaatacttg cgaagctgat ctatggtgca ctctcagtac
       61 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
      121 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
      181 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
      241 cgtgatacgc ctatttttat aggttaatgt catgatctgg atggcggcgt tagtatcgaa
      301 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
      361 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
      421 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
      481 aaacaagaat ctttttattg tcagtactga ttattccttt gccctcggac gagtgctggg
      541 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
      601 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
      661 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
      721 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
      781 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
      841 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
      901 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
      961 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     1021 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     1081 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     1141 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     1201 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     1261 ggagatgcaa taggtcaggc tctcgctgaa ttccccaatg tcaagcactt ccggaatcgg
     1321 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     1381 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     1441 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     1501 ctcgacagac gtcgcggtga gttcaggctt tttacccatg gttgtttatg ttcggatgtg
     1561 atgtgagaac tgtatcctag caagatttta aaaggaagta tatgaaagaa gaacctcagt
     1621 ggcaaatcct aaccttttat atttctctac aggggcgcgg cgtggggaca attcaacgcg
     1681 tctgtgaggg gagcgtttcc ctgctcgcag gtccgcagcg aggagccgta atttttgctt
     1741 cgcgccgtgc ggccatcaaa atgtatggat gcaaatgatt atacatgggg atgtatgggc
     1801 taaatgtacg ggcgacagtc acatcatgcc cctgagctgc gcacgtcaag actgtcaagg
     1861 agggtattct gggcctccat gtcgctggcc gggtgacccg gcggggacga ggcaagctaa
     1921 acaagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     1981 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     2041 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     2101 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     2161 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     2221 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     2281 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     2341 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     2401 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     2461 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     2521 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     2581 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     2641 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     2701 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     2761 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     2821 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     2881 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     2941 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     3001 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     3061 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3121 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3181 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     3241 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3301 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3361 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3421 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     3481 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     3541 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     3601 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     3661 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     3721 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     3781 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     3841 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     3901 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     3961 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     4021 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     4081 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctcc
     4141 accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa ttcgatatca
     4201 agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc ctatagtgag
     4261 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4321 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4381 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
     4441 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4501 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4561 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4621 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     4681 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4741 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     4801 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     4861 attttaacaa aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct
     4921 gtgcggtatt tcacaccgca tagatcaatt ccacttgcaa ttacataaaa aattccggcg
     4981 gtttttcgcg tgtgactcaa tgtcgaaata cctgcctaat gaacatgaac atcgcccaaa
     5041 tgtatttgaa gacccgctgg gagaagttca agatatataa gtaacaagca gccaatagta
     5101 taaaaaaaaa tctgagttta ttacctttcc tggaatttca gtgaaaaact gctaattata
     5161 gagagatatc acagagttac tcactaatga ctaacgaaaa ggtctggata gagaagttgg
     5221 ataatccaac tctttcagtg ttaccacatg actttttacg cccacaacaa gaaccttata
     5281 cgaaacaagc tacatattcg ttacagctac ctcagctcga tgtgcctcat gatagttttt
     5341 ctaacaaata cgctgtcgct ttgagtgtat gggctgcatt gatatataga gtaaccggtg
     5401 acgatgatat tgttctttat attgcgaata acaaaatctt aagattcaat attcaaccaa
     5461 cgtggtcatt taatgagctg tattctacaa ttaacaatga gttgaacaag ctcaattcta
     5521 ttgaggccaa tttttccttt gacgagctag ctgaaaaaat tcaaagttgc caagatctgg
     5581 aaaggacccc tcagttgttc cgtttggcct ttttggaaaa ccaagatttc aaattagacg
     5641 agttcaagca tcatttagtg gactttgctt tgaatttgga taccagtaat aatgcgcatg
     5701 ttttgaactt aatttataac agcttactgt attcgaatga aagagtaacc attgttgcgg
     5761 accaatttac tcaatatttg actgctgcgc taagcgatcc atccaattgc ataactaaaa
     5821 tctctctgat caccgcatca tccaaggata gtttacctga tccaactaag aacttgggct
     5881 ggtgcgattt cgtggggtgt attcacgaca ttttccagga caatgctgaa gccttcccag
     5941 agagaacctg tgttgtggag actccaacac taaattccga caagtcccgt tctttcactt
     6001 atcgcgacat caaccgcact tctaacatag ttgcccatta tttgattaaa acaggtatca
     6061 aaagaggtga tgtagtgatg atctattctt ctaggggtgt ggatttgatg gtatgtgtga
     6121 tgggtgtctt gaaagccggc gcaacctttt cagttatcga ccctgcatat cccccagcca
     6181 gacaaaccat ttacttaggt gttgctaaac cacgtgggtt gattgttatt agagctgctg
     6241 gacaattgga tcaactagta gaagattaca tcaatgatga attggagatt gtttcaagaa
     6301 tcaattccat cgctattcaa gaaaatggta ccattgaagg tggcaaattg gacaatggcg
     6361 aggatgtttt ggctccatat gatcactaca aagacaccag aacaggtgtt gtagttggac
     6421 cagattccaa cccaacccta tctttcacat ctggttccga aggtattcct aagggtgttc
     6481 ttggtagaca tttttccttg gcttattatt tcaattggat gtccaaaagg ttcaacttaa
     6541 cagaaaatga taaattcaca atgctgagcg gtattgcaca tgatccaatt caaagagata
     6601 tgtttacacc attattttta ggtgcccaat tgtatgtccc tactcaagat gatattggta
     6661 caccgggccg tttagcggaa tggatgagta agtatggttg cacagttacc catttaacac
     6721 ctgccatggg tcaattactt actgcccaag ctactacacc attccctaag ttacatcatg
     6781 cgttctttgt gggtgacatt ttaacaaaac gtgattgtct gaggttacaa accttggcag
     6841 aaaattgccg tattgttaat atgtacggta ccactgaaac acagcgtgca gtttcttatt
     6901 tcgaagttaa atcaaaaaat gacgatccaa actttttgaa aaaattgaaa gatgtcatgc
     6961 ctgctggtaa aggtatgttg aacgttcagc tactagttgt taacaggaac gatcgtactc
     7021 aaatatgtgg tattggcgaa ataggtgaga tttatgttcg tgcaggtggt ttggccgaag
     7081 gttatagagg attaccagaa ttgaataaag aaaaatttgt gaacaactgg tttgttgaaa
     7141 aagatcactg gaattatttg gataaggata atggtgaacc ttggagacaa ttctggttag
     7201 gtccaagaga tagattgtac agaacgggtg atttaggtcg ttatctacca aacggtgact
     7261 gtgaatgttg cggtagggct gatgatcaag ttaaaattcg tgggttcaga atcgaattag
     7321 gagaaataga tacgcacatt tcccaacatc cattggtaag agaaaacatt actttagttc
     7381 gcaaaaatgc cgacaatgag ccaacattga tcacatttat ggtcccaaga tttgacaagc
     7441 cagatgactt gtctaagttc caaagtgatg ttccaaagga ggttgaaact gaccctatag
     7501 ttaagggctt aatcggttac catcttttat ccaaggacat caggactttc ttaaagaaaa
     7561 gattggctag ctatgctatg ccttccttga ttgtggttat ggataaacta ccattgaatc
     7621 caaatggtaa agttgataag cctaaacttc aattcccaac tcccaagcaa ttaaatttgg
     7681 tagctgaaaa tacagtttct gaaactgacg actctcagtt taccaatgtt gagcgcgagg
     7741 ttagagactt atggttaagt atattaccta ccaagccagc atctgtatca ccagatgatt
     7801 cgtttttcga tttaggtggt cattctatct tggctaccaa aatgattttt accttaaaga
     7861 aaaagctgca agttgattta ccattgggca caattttcaa gtatccaacg ataaaggcct
     7921 ttgccgcgga aattgacaga attaaatcat cgggtggatc atctcaaggt gaggtcgtcg
     7981 aaaatgtcac tgcaaattat gcggaagacg ccaagaaatt ggttgagacg ctaccaagtt
     8041 cgtacccctc tcgagaatat tttgttgaac ctaatagtgc cgaaggaaaa acaacaatta
     8101 atgtgtttgt taccggtgtc acaggatttc tgggctccta catccttgca gatttgttag
     8161 gacgttctcc aaagaactac agtttcaaag tgtttgccca cgtcagggcc aaggatgaag
     8221 aagctgcatt tgcaagatta caaaaggcag gtatcaccta tggtacttgg aacgaaaaat
     8281 ttgcctcaaa tattaaagtt gtattaggcg atttatctaa aagccaattt ggtctttcag
     8341 atgagaagtg gatggatttg gcaaacacag ttgatataat tatccataat ggtgcgttag
     8401 ttcactgggt ttatccatat gccaaattga gggatccaaa tgttatttca actatcaatg
     8461 ttatgagctt agccgccgtc ggcaagccaa agttctttga ctttgtttcc tccacttcta
     8521 ctcttgacac tgaatactac tttaatttgt cagataaact tgttagcgaa gggaagccag
     8581 gcattttaga atcagacgat ttaatgaact ctgcaagcgg gctcactggt ggatatggtc
     8641 agtccaaatg ggctgctgag tacatcatta gacgtgcagg tgaaaggggc ctacgtgggt
     8701 gtattgtcag accaggttac gtaacaggtg cctctgccaa tggttcttca aacacagatg
     8761 atttcttatt gagatttttg aaaggttcag tccaattagg taagattcca gatatcgaaa
     8821 attccgtgaa tatggttcca gtagatcatg ttgctcgtgt tgttgttgct acgtctttga
     8881 atcctcccaa agaaaatgaa ttggccgttg ctcaagtaac gggtcaccca agaatattat
     8941 tcaaagacta cttgtatact ttacacgatt atggttacga tgtcgaaatc gaaagctatt
     9001 ctaaatggaa gaaatcattg gaggcgtctg ttattgacag gaatgaagaa aatgcgttgt
     9061 atcctttgct acacatggtc ttagacaact tacctgaaag taccaaagct ccggaactag
     9121 acgataggaa cgccgtggca tctttaaaga aagacaccgc atggacaggt gttgattggt
     9181 ctaatggaat aggtgttact ccagaagagg ttggtatata tattgcattt ttaaacaagg
     9241 ttggattttt acctccacca actcataatg acaaacttcc actgccaagt atagaactaa
     9301 ctcaagcgca aataagtcta gttgcttcag gtgctggtgc tcgtggaagc tccgcagcag
     9361 cttaaggttg agcattacgt atgatatgtc catgtacaat aattaaatat gaattaggag
     9421 aaagacttag cttcttttcg ggtgatgtca cttaaaaact ccgagaataa tatataataa
     9481 gagaataaaa tattagttat tgaataagaa ctgtaaatca gctggcgtta gtctgctaat
     9541 ggcagcttca tcttggttta ttgtagcatg aatcatattt gccttttttt cctgtaattc
     9601 aatgattctt gcttctatac tatcctcaat gcaaaacctt gtgatcttca caggtcgata
     9661 ctgaccaatt ctatgaactc tatcaccact ttgccattca acactagg
//
