LOCUS       sequence-239187-        9252 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  LYS2-NatMx integrating (YIp) vector for stable genomic integration 
            in the BY4741 background..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9252)
  AUTHORS   Flagg MP, Kao A, Hampton RY
  TITLE     ICE plasmids: combining the ease of yeast recombination cloning with
            the stability of genomic integration.
  JOURNAL   Yeast. 2019 May 10. doi: 10.1002/yea.3400.
  PUBMED    31074531
REFERENCE   2  (bases 1 to 9252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2019
            May 10. doi: 10.1002/yea.3400."
COMMENT     Sequence Label: YIp LYS2-NatMx
FEATURES             Location/Qualifiers
     source          1..9252
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(125..144)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     244..266
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(304..322)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     gene            complement(375..1494)
                     /label=natMX6
                     /note="yeast selectable marker conferring nourseothricin 
                     resistance"
     terminator      complement(375..572)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(578..1150)
                     /codon_start=1
                     /gene="Streptomyces noursei nat1"
                     /product="nourseothricin acetyltransferase"
                     /label=NrsR
                     /note="confers resistance to nourseothricin"
                     /translation="MGTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDDGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     promoter        complement(1151..1494)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     promoter        1567..1671
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1672..1740,1741..2532)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1890..1909)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2703..3291
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3192..3211
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3445..3462
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3579..3600
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3615..3620,3621..3638,3639..3645)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3653..3669
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3658..3680
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3677..3693
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3677..3693
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3689..4069
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWTRPVAAH"
     primer_bind     3712..3732
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3714..3732
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    3745..3852
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     primer_bind     3769..3785
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3769..3785
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3819..3835)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3820..3836)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(3860..3879)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3861..3879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3889..3906)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3889..3905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3898..3920)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4046..4501
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4133..4152)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4343..4364
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4558..4797
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     CDS             4798..8976
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
ORIGIN
        1 tgatcttcac aggtcgatac tgaccaattc tatgaactct atcaccactt tgccattcaa
       61 cactagggtt ccaccatggg tctaaaatga atacttgcga agctgatcta tggtgcactc
      121 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      181 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
      241 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
      301 agggcctcgt gatacgccta tttttatagg ttaatgtcat gatctggatg gcggcgttag
      361 tatcgaatcg acagcagtat agcgaccagc attcacatac gattgacgca tgatattact
      421 ttctgcgcac ttaacttcgc atctgggcag atgatgtcga ggcgaaaaaa aatataaatc
      481 acgctaacat ttgattaaaa tagaacaact acaatataaa aaaactatac aaatgacaag
      541 ttcttgaaaa caagaatctt tttattgtca gtactgatta ggggcagggc atgctcatgt
      601 agagcgcctg ctcgccgtcc gaggcggtgc cgtcgtacag ggcggtgtcc aggccgcaga
      661 gggtgaaccc catccgccgg tacgcgtgga tcgccggtgc gttgacgttg gtgacctcca
      721 gccagaggtg cccggcgccc cgctcgcggg cgaactccgt cgcgagcccc atcaacgcgc
      781 gcccgacccc gtgcccccgg tgctccgggg cgacctcgat gtcctcgacg gtcagccggc
      841 ggttccagcc ggagtacgag acgaccacga agcccgccag gtcgccgtcg tccccgtacg
      901 cgacgaacgt ccgggagtcc gggtcgccgt cctccccgtc gtccgattcg tcgtccgatt
      961 cgtcgtcggg gaacaccttg gtcaggggcg ggtccaccgg cacctcccgc agggtgaagc
     1021 cgtccccggt ggcggtgacg cggaagacgg tgtcggtggt gaaggaccca tccagtgcct
     1081 cgatggcctc ggcgtccccc gggacactgg tgcggtaccg gtaagccgtg tcgtcaagag
     1141 tggtacccat ggttgtttat gttcggatgt gatgtgagaa ctgtatccta gcaagatttt
     1201 aaaaggaagt atatgaaaga agaacctcag tggcaaatcc taacctttta tatttctcta
     1261 caggggcgcg gcgtggggac aattcaacgc gtctgtgagg ggagcgtttc cctgctcgca
     1321 ggtctgcagc gaggagccgt aatttttgct tcgcgccgtg cggccatcaa aatgtatgga
     1381 tgcaaatgat tatacatggg gatgtatggg ctaaatgtac gggcgacagt cacatcatgc
     1441 ccctgagctg cgcacgtcaa gactgtcaag gagggtattc tgggcctcca tgtcgctggc
     1501 cgggtgaccc ggcggggacg aggcaagcta aacaagacgt caggtggcac ttttcgggga
     1561 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1621 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1681 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1741 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1801 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1861 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1921 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     1981 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2041 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2101 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2161 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2221 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2281 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2341 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2401 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2461 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2521 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2581 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2641 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2701 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2761 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2821 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2881 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2941 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3001 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3061 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3121 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3181 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3241 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3301 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3361 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3421 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3481 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3541 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3601 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3661 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     3721 cctcactaaa gggaacaaaa gctggagctc caccgcggtg gcggccgctc tagaactagt
     3781 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     3841 gggcccggta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     3901 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     3961 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     4021 gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4081 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4141 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4201 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4261 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4321 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4381 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4441 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4501 tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagatcaat
     4561 tccacttgca attacataaa aaattccggc ggtttttcgc gtgtgactca atgtcgaaat
     4621 acctgcctaa tgaacatgaa catcgcccaa atgtatttga agacccgctg ggagaagttc
     4681 aagatatata agtaacaagc agccaatagt ataaaaaaaa atctgagttt attacctttc
     4741 ctggaatttc agtgaaaaac tgctaattat agagagatat cacagagtta ctcactaatg
     4801 actaacgaaa aggtctggat agagaagttg gataatccaa ctctttcagt gttaccacat
     4861 gactttttac gcccacaaca agaaccttat acgaaacaag ctacatattc gttacagcta
     4921 cctcagctcg atgtgcctca tgatagtttt tctaacaaat acgctgtcgc tttgagtgta
     4981 tgggctgcat tgatatatag agtaaccggt gacgatgata ttgttcttta tattgcgaat
     5041 aacaaaatct taagattcaa tattcaacca acgtggtcat ttaatgagct gtattctaca
     5101 attaacaatg agttgaacaa gctcaattct attgaggcca atttttcctt tgacgagcta
     5161 gctgaaaaaa ttcaaagttg ccaagatctg gaaaggaccc ctcagttgtt ccgtttggcc
     5221 tttttggaaa accaagattt caaattagac gagttcaagc atcatttagt ggactttgct
     5281 ttgaatttgg ataccagtaa taatgcgcat gttttgaact taatttataa cagcttactg
     5341 tattcgaatg aaagagtaac cattgttgcg gaccaattta ctcaatattt gactgctgcg
     5401 ctaagcgatc catccaattg cataactaaa atctctctga tcaccgcatc atccaaggat
     5461 agtttacctg atccaactaa gaacttgggc tggtgcgatt tcgtggggtg tattcacgac
     5521 attttccagg acaatgctga agccttccca gagagaacct gtgttgtgga gactccaaca
     5581 ctaaattccg acaagtcccg ttctttcact tatcgcgaca tcaaccgcac ttctaacata
     5641 gttgcccatt atttgattaa aacaggtatc aaaagaggtg atgtagtgat gatctattct
     5701 tctaggggtg tggatttgat ggtatgtgtg atgggtgtct tgaaagccgg cgcaaccttt
     5761 tcagttatcg accctgcata tcccccagcc agacaaacca tttacttagg tgttgctaaa
     5821 ccacgtgggt tgattgttat tagagctgct ggacaattgg atcaactagt agaagattac
     5881 atcaatgatg aattggagat tgtttcaaga atcaattcca tcgctattca agaaaatggt
     5941 accattgaag gtggcaaatt ggacaatggc gaggatgttt tggctccata tgatcactac
     6001 aaagacacca gaacaggtgt tgtagttgga ccagattcca acccaaccct atctttcaca
     6061 tctggttccg aaggtattcc taagggtgtt cttggtagac atttttcctt ggcttattat
     6121 ttcaattgga tgtccaaaag gttcaactta acagaaaatg ataaattcac aatgctgagc
     6181 ggtattgcac atgatccaat tcaaagagat atgtttacac cattattttt aggtgcccaa
     6241 ttgtatgtcc ctactcaaga tgatattggt acaccgggcc gtttagcgga atggatgagt
     6301 aagtatggtt gcacagttac ccatttaaca cctgccatgg gtcaattact tactgcccaa
     6361 gctactacac cattccctaa gttacatcat gcgttctttg tgggtgacat tttaacaaaa
     6421 cgtgattgtc tgaggttaca aaccttggca gaaaattgcc gtattgttaa tatgtacggt
     6481 accactgaaa cacagcgtgc agtttcttat ttcgaagtta aatcaaaaaa tgacgatcca
     6541 aactttttga aaaaattgaa agatgtcatg cctgctggta aaggtatgtt gaacgttcag
     6601 ctactagttg ttaacaggaa cgatcgtact caaatatgtg gtattggcga aataggtgag
     6661 atttatgttc gtgcaggtgg tttggccgaa ggttatagag gattaccaga attgaataaa
     6721 gaaaaatttg tgaacaactg gtttgttgaa aaagatcact ggaattattt ggataaggat
     6781 aatggtgaac cttggagaca attctggtta ggtccaagag atagattgta cagaacgggt
     6841 gatttaggtc gttatctacc aaacggtgac tgtgaatgtt gcggtagggc tgatgatcaa
     6901 gttaaaattc gtgggttcag aatcgaatta ggagaaatag atacgcacat ttcccaacat
     6961 ccattggtaa gagaaaacat tactttagtt cgcaaaaatg ccgacaatga gccaacattg
     7021 atcacattta tggtcccaag atttgacaag ccagatgact tgtctaagtt ccaaagtgat
     7081 gttccaaagg aggttgaaac tgaccctata gttaagggct taatcggtta ccatctttta
     7141 tccaaggaca tcaggacttt cttaaagaaa agattggcta gctatgctat gccttccttg
     7201 attgtggtta tggataaact accattgaat ccaaatggta aagttgataa gcctaaactt
     7261 caattcccaa ctcccaagca attaaatttg gtagctgaaa atacagtttc tgaaactgac
     7321 gactctcagt ttaccaatgt tgagcgcgag gttagagact tatggttaag tatattacct
     7381 accaagccag catctgtatc accagatgat tcgtttttcg atttaggtgg tcattctatc
     7441 ttggctacca aaatgatttt taccttaaag aaaaagctgc aagttgattt accattgggc
     7501 acaattttca agtatccaac gataaaggcc tttgccgcgg aaattgacag aattaaatca
     7561 tcgggtggat catctcaagg tgaggtcgtc gaaaatgtca ctgcaaatta tgcggaagac
     7621 gccaagaaat tggttgagac gctaccaagt tcgtacccct ctcgagaata ttttgttgaa
     7681 cctaatagtg ccgaaggaaa aacaacaatt aatgtgtttg ttaccggtgt cacaggattt
     7741 ctgggctcct acatccttgc agatttgtta ggacgttctc caaagaacta cagtttcaaa
     7801 gtgtttgccc acgtcagggc caaggatgaa gaagctgcat ttgcaagatt acaaaaggca
     7861 ggtatcacct atggtacttg gaacgaaaaa tttgcctcaa atattaaagt tgtattaggc
     7921 gatttatcta aaagccaatt tggtctttca gatgagaagt ggatggattt ggcaaacaca
     7981 gttgatataa ttatccataa tggtgcgtta gttcactggg tttatccata tgccaaattg
     8041 agggatccaa atgttatttc aactatcaat gttatgagct tagccgccgt cggcaagcca
     8101 aagttctttg actttgtttc ctccacttct actcttgaca ctgaatacta ctttaatttg
     8161 tcagataaac ttgttagcga agggaagcca ggcattttag aatcagacga tttaatgaac
     8221 tctgcaagcg ggctcactgg tggatatggt cagtccaaat gggctgctga gtacatcatt
     8281 agacgtgcag gtgaaagggg cctacgtggg tgtattgtca gaccaggtta cgtaacaggt
     8341 gcctctgcca atggttcttc aaacacagat gatttcttat tgagattttt gaaaggttca
     8401 gtccaattag gtaagattcc agatatcgaa aattccgtga atatggttcc agtagatcat
     8461 gttgctcgtg ttgttgttgc tacgtctttg aatcctccca aagaaaatga attggccgtt
     8521 gctcaagtaa cgggtcaccc aagaatatta ttcaaagact acttgtatac tttacacgat
     8581 tatggttacg atgtcgaaat cgaaagctat tctaaatgga agaaatcatt ggaggcgtct
     8641 gttattgaca ggaatgaaga aaatgcgttg tatcctttgc tacacatggt cttagacaac
     8701 ttacctgaaa gtaccaaagc tccggaacta gacgatagga acgccgtggc atctttaaag
     8761 aaagacaccg catggacagg tgttgattgg tctaatggaa taggtgttac tccagaagag
     8821 gttggtatat atattgcatt tttaaacaag gttggatttt tacctccacc aactcataat
     8881 gacaaacttc cactgccaag tatagaacta actcaagcgc aaataagtct agttgcttca
     8941 ggtgctggtg ctcgtggaag ctccgcagca gcttaaggtt gagcattacg tatgatatgt
     9001 ccatgtacaa taattaaata tgaattagga gaaagactta gcttcttttc gggtgatgtc
     9061 acttaaaaac tccgagaata atatataata agagaataaa atattagtta ttgaataaga
     9121 actgtaaatc agctggcgtt agtctgctaa tggcagcttc atcttggttt attgtagcat
     9181 gaatcatatt tgcctttttt tcctgtaatt caatgattct tgcttctata ctatcctcaa
     9241 tgcaaaacct tg
//
