LOCUS       sequence-239188-       12751 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a dominant negative Kash control, that lacks the actual 
            Kash domain for LINC complex integration, in addition to 
            fluorescently tagged (mRuby2) alpha-Actinin-2 on an adenoviral 
            backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12751)
  AUTHORS   Seelbinder B, Ghosh S, Schneider SE, Scott AK, Berman AG, Goergen 
            CJ, Margulies KB, Bedi KC Jr, Casas E, Swearingen AR, Brumbaugh J, 
            Calve S, Neu CP
  TITLE     Nuclear deformation guides chromatin reorganization in cardiac 
            development and disease.
  JOURNAL   Nat Biomed Eng. 2021 Dec;5(12):1500-1516. doi: 
            10.1038/s41551-021-00823-9. Epub 2021 Dec 2.
  PUBMED    34857921
REFERENCE   2  (bases 1 to 12751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41551-021-00823-9"; journalName: "Nat Biomed Eng"; date: 
            "2021-12"; volume: "5"; issue: "12"; pages: "1500-1516"
COMMENT     Sequence Label: Ad:2CMV_A2R2-N25Ctr
FEATURES             Location/Qualifiers
     source          1..12751
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        122..425
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        426..629
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             3371..4081
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4243..4364
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   7555..7657
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     primer_bind     complement(7746..7763)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7917..8505)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7997..8016)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             9337..10131
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9337..10131
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9391..9410)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10001..10020
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      complement(10179..10559)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10241..10262)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10453..10472
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   10597..10699
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     10787..10937
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     enhancer        10995..11298
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11299..11502
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             11624..12355
                     /codon_start=1
                     /product="brighter version of the far-red fluorescent 
                     protein mNeptune2 with slightly blue-shifted spectra"
                     /label=mNeptune2.5
                     /translation="MVSKGEELIKENMHTKLYMEGTVNNHHFKCTHEGEGKPYEGTQTN
                     RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRCD
                     MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     polyA_signal    join(12654..12751,1..24)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 gtccaaactc atcaatgtat cttaacgcgg atctgggcgt ggttaatatt tcatagcgcg
       61 taatactgta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
      121 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
      181 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
      241 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
      301 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
      361 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
      421 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
      481 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
      541 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
      601 tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagaggc
      661 tagcaccatg aatcagatag agcccggcgt gcagtacaac tatgtctacg acgaagatga
      721 gtatatgatc caggaggagg agtgggaccg cgacctgctc ctcgacccgg cctgggagaa
      781 gcagcagagg aagacgttca ctgcctggtg caactcacac ctgcggaaag ctggcaccca
      841 gatcgagaac atcgaggagg atttcaggaa cggcctcaag ctcatgctac ttctagaagt
      901 catctcaggg gaaaggctgc ccaaacctga ccggggaaaa atgcggttcc acaagattgc
      961 gaatgtcaac aaggctctgg attacatagc cagcaaggga gtgaagctgg tatctattgg
     1021 agctgaagaa atagtcgatg gcaatgtgaa gatgaccctg ggtatgatct ggaccatcat
     1081 cctccgcttc gccattcagg acatctcagt ggaagaaaca tctgccaaag aagggctgct
     1141 gctatggtgt cagaggaaga cagctccata cagaaatgtg aatatccaga acttccacac
     1201 cagctggaaa gatggccttg gactctgtgc cctcatccac agacaccggc ccgacctcat
     1261 tgactactca aagcttaaca aggatgaccc cataggaaac attaacctgg ccatggaaat
     1321 agcagagaag cacttggata tccctaagat gttggatgct gaagacattg tcaacactcc
     1381 caaacccgat gaaagagcca tcatgactta cgtttcctgc ttctaccatg ctttcgcggg
     1441 agcggagcag gccgagacag cagctaacag aatatgtaag gttcttgctg tgaatcaaga
     1501 gaatgagagg ctgatggaag aatatgagag gctagcgagt gagctgttgg agtggattcg
     1561 gcgcacaatc ccctggctgg agaacaggac gcctgagaag accatgcagg ccatgcagaa
     1621 gaagctggag gacttccggg attaccgtcg taagcataag ccccccaagg tgcaggagaa
     1681 gtgccagctg gagatcaact tcaacacgct gcagaccaag cttcggatca gcaaccggcc
     1741 cgccttcatg ccctccgagg gcaagatggt gtcggacatt gctggtgcct ggcagaggtt
     1801 ggagcaggct gagaagggct atgaggagtg gctattgaat gagattcgaa gactggagcg
     1861 cttggagcac ttggctgaga agttcaggca gaaggcctcc acgcacgaga catgggctta
     1921 tggcaaagaa cagatcttgc tgcagaagga ctacgagtct gcgtccctga cggaagttcg
     1981 agccctactt cggaagcacg aggcctttga gagcgacgtg gctgcacacc aggaccgcgt
     2041 ggagcagatc gctgccattg cgcaggagct caatgaactg gactatcacg atgctgtgaa
     2101 cgtcaatgat cggtgccaga aaatttgcga ccagtgggat agattgggga cgctcaccca
     2161 gaagaggagg gaggccttgg agagaactga gaagttgctg gaaacaatcg accaactgca
     2221 cctggagttt gccaagagag ccgcgccctt caacaactgg atggagggag ccatggagga
     2281 cctgcaggac atgttcattg tccacagcat cgaggagatc cagagtctga tcactgcaca
     2341 tgagcagttc aaggccacac tgcccgaggc cgacggggag cggcagtcca tcctggccat
     2401 ccagaacgag gtggagaagg tgatccagag ctacagcatc cggatcagct ccagcaaccc
     2461 ctacagcact gtcaccatgg atgagctgcg caacaagtgg gacaaggtga agcagctggt
     2521 gccggtccgg gatcagtccc tgcaggagga gctggctcgc caacatgcta atgagcgtct
     2581 gcggcgccag tttgctgctc aggccaatgc cattgggccc tggatccaga acaagatgga
     2641 ggagatcgct cggagctcca tccagatcac cggggccctg gaggaccaga tgaaccagct
     2701 gaagcagtac gaacacaaca tcatcaacta caagaacaac atcgacaagc tggagggcga
     2761 ccaccagctc atccaggagg ccctggtctt cgacaacaag cacacgaact acaccatgga
     2821 gcatatccgt gtcggatggg agttgctgct gacaactatc gccagaacca tcaatgaggt
     2881 ggagacccag atcctgacaa gagatgccaa gggcatcacc caggagcaga tgaatgagtt
     2941 cagagcctcc ttcaaccact ttgacaggag gaagaacggc ctgatggatc atgaggattt
     3001 cagagcctgc ctcatttcca tgggctatga cttgggtgaa gctgaatttg cccgcattat
     3061 gactttggtt gaccccaacg gacaaggcac ggtcaccttc cagtccttca ttgacttcat
     3121 gactagagag accgcagaca ccgacacggc agagcaggtc atcgcctcct tccggattct
     3181 ggcttctgat aagccttaca tcttggcaga ggagcttcgt cgagagctgc ctccggatca
     3241 ggcccagtac tgcatcaaga gaatgccccc atactcaggc ccgggcagtg tccccggggc
     3301 cctggactac actgccttct cctctgccct ctacggggaa agcgacctcg gcgaccctcc
     3361 tgttgcaacg atggtgtcta agggcgaaga gctgatcaag gaaaatatgc gtatgaaggt
     3421 ggtcatggaa ggttcggtca acggccacca attcaaatgc acaggtgaag gagaaggcaa
     3481 tccgtacatg ggaactcaaa ccatgaggat caaagtcatc gagggaggac ccctgccatt
     3541 tgcctttgac attcttgcca cgtcgttcat gtatggcagc cgtactttta tcaagtaccc
     3601 gaaaggcatt cctgatttct ttaaacagtc ctttcctgag ggttttactt gggaaagagt
     3661 tacgagatac gaagatggtg gagtcgtcac cgtcatgcag gacaccagcc ttgaggatgg
     3721 ctgtctcgtt taccacgtcc aagtcagagg ggtaaacttt ccctccaatg gtcccgtgat
     3781 gcagaagaag accaagggtt gggagcctaa tacagagatg atgtatccag cagatggtgg
     3841 tctgagggga tacactcata tggcactgaa agttgatggt ggtggccatc tgtcttgctc
     3901 tttcgtaaca acttacaggt caaaaaagac cgtcgggaac atcaagatgc ccggtatcca
     3961 tgccgttgat caccgcctgg aaaggttaga ggaaagtgac aatgaaatgt tcgtagtaca
     4021 acgcgaacac gcagttgcca agttcgccgg gcttggtggt gggatggacg agctgtacaa
     4081 gtaactcgag cctaagcttc tagataagat atccgatcca ccggatctag ataactgatc
     4141 ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     4201 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     4261 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     4321 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttaacgcgg atctgggcgt
     4381 ggttaagggt gggaaagaat atataaggtg ggggtcttat gtagttttgt atctgttttg
     4441 cagcagccgc cgccgccatg agcaccaact cgtttgatgg aagcattgtg agctcatatt
     4501 tgacaacgcg catgccccca tgggccgggg tgcgtcagaa tgtgatgggc tccagcattg
     4561 atggtcgccc cgtcctgccc gcaaactcta ctaccttgac ctacgagacc gtgtctggaa
     4621 cgccgttgga gactgcagcc tccgccgccg cttcagccgc tgcagccacc gcccgcggga
     4681 ttgtgactga ctttgctttc ctgagcccgc ttgcaagcag tgcagcttcc cgttcatccg
     4741 cccgcgatga caagttgacg gctcttttgg cacaattgga ttctttgacc cgggaactta
     4801 atgtcgtttc tcagcagctg ttggatctgc gccagcaggt ttctgccctg aaggcttcct
     4861 cccctcccaa tgcggtttaa aacataaata aaaaaccaga ctctgtttgg atttggatca
     4921 agcaagtgtc ttgctgtctt tatttagggg ttttgcgcgc gcggtaggcc cgggaccagc
     4981 ggtctcggtc gttgagggtc ctgtgtattt tttccaggac gtggtaaagg tgactctgga
     5041 tgttcagata catgggcata agcccgtctc tggggtggag gtagcaccac tgcagagctt
     5101 catgctgcgg ggtggtgttg tagatgatcc agtcgtagca ggagcgctgg gcgtggtgcc
     5161 taaaaatgtc tttcagtagc aagctgattg ccaggggcag gcccttggtg taagtgttta
     5221 caaagcggtt aagctgggat gggtgcatac gtggggatat gagatgcatc ttggactgta
     5281 tttttaggtt ggctatgttc ccagccatat ccctccgggg attcatgttg tgcagaacca
     5341 ccagcacagt gtatccggtg cacttgggaa atttgtcatg tagcttagaa ggaaatgcgt
     5401 ggaagaactt ggagacgccc ttgtgacctc caagattttc catgcattcg tccataatga
     5461 tggcaatggg cccacgggcg gcggcctggg cgaagatatt tctgggatca ctaacgtcat
     5521 agttgtgttc caggatgaga tcgtcatagg ccatttttac aaagcgcggg cggagggtgc
     5581 cagactgcgg tataatggtt ccatccggcc caggggcgta gttaccctca cagatttgca
     5641 tttcccacgc tttgagttca gatgggggga tcatgtctac ctgcggggcg atgaagaaaa
     5701 cggtttccgg ggtaggggag atcagctggg aagaaagcag gttcctgagc agctgcgact
     5761 taccgcagcc ggtgggcccg taaatcacac ctattaccgg ctgcaactgg tagttaagag
     5821 agctgcagct gccgtcatcc ctgagcaggg gggccacttc gttaagcatg tccctgactc
     5881 gcatgttttc cctgaccaaa tccgccagaa ggcgctcgcc gcccagcgat agcagttctt
     5941 gcaaggaagc aaagtttttc aacggtttga gaccgtccgc cgtaggcatg cttttgagcg
     6001 tttgaccaag cagttccagg cggtcccaca gctcggtcac ctgctctacg gcatctcgat
     6061 ccagcatatc tcctcgtttc gcgggttggg gcggctttcg ctgtacggca gtagtcggtg
     6121 ctcgtccaga cgggccaggg tcatgtcttt ccacgggcgc agggtcctcg tcagcgtagt
     6181 ctgggtcacg gtgaaggggt gcgctccggg ctgcgcgctg gccagggtgc gcttgaggct
     6241 ggtcctgctg gtgctgaagc gctgccggtc ttcgccctgc gcgtcggcca ggtagcattt
     6301 gaccatggtg tcatagtcca gcccctccgc ggcgtggccc ttggcgcgca gcttgccctt
     6361 ggaggaggcg ccgcacgagg ggcagtgcag acttttgagg gcgtagagct tgggcgcgag
     6421 aaataccgat tccggggagt aggcatccgc gccgcaggcc ccgcagacgg tctcgcattc
     6481 cacgagccag gtgagctctg gccgttcggg gtcaaaaacc aggtttcccc catgcttttt
     6541 gatgcgtttc ttacctctgg tttccatgag ccggtgtcca cgctcggtga cgaaaaggct
     6601 gtccgtgtcc ccgtatacag acttgagagg gagtttaaac gaattcaata gcttgttgca
     6661 tgggcggcga tataaaatgc aaggtgctgc tcaaaaaatc aggcaaagcc tcgcgcaaaa
     6721 aagaaagcac atcgtagtca tgctcatgca gataaaggca ggtaagctcc ggaaccacca
     6781 cagaaaaaga caccattttt ctctcaaaca tgtctgcggg tttctgcata aacacaaaat
     6841 aaaataacaa aaaaacattt aaacattaga agcctgtctt acaacaggaa aaacaaccct
     6901 tataagcata agacggacta cggccatgcc ggcgtgaccg taaaaaaact ggtcaccgtg
     6961 attaaaaagc accaccgaca gctcctcggt catgtccgga gtcataatgt aagactcggt
     7021 aaacacatca ggttgattca catcggtcag tgctaaaaag cgaccgaaat agcccggggg
     7081 aatacatacc cgcaggcgta gagacaacat tacagccccc ataggaggta taacaaaatt
     7141 aataggagag aaaaacacat aaacacctga aaaaccctcc tgcctaggca aaatagcacc
     7201 ctcccgctcc agaacaacat acagcgcttc cacagcggca gccataacag tcagccttac
     7261 cagtaaaaaa gaaaacctat taaaaaaaca ccactcgaca cggcaccagc tcaatcagtc
     7321 acagtgtaaa aaagggccaa gtgcagagcg agtatatata ggactaaaaa atgacgtaac
     7381 ggttaaagtc cacaaaaaac acccagaaaa ccgcacgcga acctacgccc agaaacgaaa
     7441 gccaaaaaac ccacaacttc ctcaaatcgt cacttccgtt ttcccacgtt acgtcacttc
     7501 ccattttaag aaaactacaa ttcccaacac atacaagtta ctccgcccta aaacctacgt
     7561 cacccgcccc gttcccacgc cccgcgccac gtcacaaact ccaccccctc attatcatat
     7621 tggcttcaat ccaaaataag gtatattatt gatgatgtta attaacatgc atggatccat
     7681 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc
     7741 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     7801 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7861 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7921 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7981 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     8041 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     8101 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     8161 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     8221 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     8281 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     8341 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     8401 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     8461 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     8521 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     8581 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     8641 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     8701 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     8761 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     8821 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8881 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8941 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcagccatg
     9001 agattatcaa aaaggatctt cacctagatc cttttcacgt agaaagccag tccgcagaaa
     9061 cggtgctgac cccggatgaa tgtcagctac tgggctatct ggacaaggga aaacgcaagc
     9121 gcaaagagaa agcaggtagc ttgcagtggg cttacatggc gatagctaga ctgggcggtt
     9181 ttatggacag caagcgaacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     9241 ccctgcaaag taaactggat ggctttcttg ccgccaagga tctgatggcg caggggatca
     9301 agctctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga tggattgcac
     9361 gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc acaacagaca
     9421 atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt
     9481 gtcaagaccg acctgtccgg tgccctgaat gaactgcaag acgaggcagc gcggctatcg
     9541 tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac tgaagcggga
     9601 agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct
     9661 cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac gcttgatccg
     9721 gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg tactcggatg
     9781 gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct cgcgccagcc
     9841 gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg aggatctcgt cgtgacccat
     9901 ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg attcatcgac
     9961 tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac ccgtgatatt
    10021 gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg tatcgccgct
    10081 cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg aattttgtta
    10141 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
    10201 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
    10261 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
    10321 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
    10381 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
    10441 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
    10501 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
    10561 cattcgccat tcaggatcga attaattctt aattaacatc atcaataata taccttattt
    10621 tggattgaag ccaatatgat aatgaggggg tggagtttgt gacgtggcgc ggggcgtggg
    10681 aacggggcgg gtgacgtagt agtgtggcgg aagtgtgatg ttgcaagtgt ggcggaacac
    10741 atgtaagcga cggatgtggc aaaagtgacg tttttggtgt gcgccggtgt acacaggaag
    10801 tgacaatttt cgcgcggttt taggcggatg ttgtagtaaa tttgggcgta accgagtaag
    10861 atttggccat tttcgcggga aaactgaata agaggaagtg aaatctgaat aattttgtgt
    10921 tactcatagc gcgtaatact gtaatagtaa tcaattacgg ggtcattagt tcatagccca
    10981 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
    11041 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
    11101 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
    11161 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
    11221 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
    11281 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
    11341 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
    11401 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
    11461 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag
    11521 atccgctaga gatctggtac cgtcgacgcg gccgctcgac accatgaaac taggacgggc
    11581 cgtgctgggc ctgctgctgc tggcgccgtc cgtggtgcag gcgatggtga gcaagggcga
    11641 ggagctgatc aaggagaaca tgcacacgaa gctgtacatg gaaggcaccg tgaacaacca
    11701 ccacttcaag tgcacccacg aaggggaggg caagccctac gagggcaccc agaccaacag
    11761 gattaaggtg gtggagggag gccccctgcc gttcgcattc gacatcctgg ccacctgctt
    11821 tatgtacggg agcaagacct tcatcaacca cacccagggc atccccgatt tctttaagca
    11881 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
    11941 taccgttacc caggacacca gcctccagga cggctgcttg atctacaacg tcaagctcag
    12001 aggggtgaac ttcccatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
    12061 cagtaccgag accctgtacc ccgctgacgg cggcctggaa ggcagatgcg acatggccct
    12121 gaagctcgtg ggcgggggcc acctgcactg caacctgaag accacataca gatccaagaa
    12181 acccgctaag aacctcaaga tgcccggcgt ctactttgtg gaccgcagac tggaaagaat
    12241 caaggaggcc gacaatgaga cctacgtcga gcagcacgag gtggctgtgg ccagatactg
    12301 cgacctccct agcaaactgg ggcacaaact taatggcatg gacgagctgt acaaggggga
    12361 tccaccggtc gccaccagtc ggaggcagaa gcggtggagt ccctgctctc tcctacagaa
    12421 agcatgccgt gtggcactgc cattgcagct gttgctcctg ctctttctgc tgctgctgtt
    12481 cctgctgccg gcctagggta ccctaagctt ctagataaga tatccgatcc accggatcta
    12541 gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc
    12601 tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt
    12661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
    12721 catttttttc actgcattct agttgtggtt t
//
