LOCUS       sequence-239201-       17227 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Abscisic acid-inducible B4GALT1 expression in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17227)
  AUTHORS   Chang MM, Gaidukov L, Jung G, Tseng WA, Scarcelli JJ, Cornell R, 
            Marshall JK, Lyles JL, Sakorafas P, Chu AA, Cote K, Tzvetkova B, 
            Dolatshahi S, Sumit M, Mulukutla BC, Lauffenburger DA, Figueroa B 
            Jr, Summers NM, Lu TK, Weiss R
  TITLE     Small-molecule control of antibody N-glycosylation in engineered 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4.
  PUBMED    31110306
REFERENCE   2  (bases 1 to 17227)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2019 May 20. pii: 10.1038/s41589-019-0288-4. doi: 
            10.1038/s41589-019-0288-4."
COMMENT     Sequence Label: B4GALT1-ABA
FEATURES             Location/Qualifiers
     source          1..17227
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    4..41
                     /label=Bxb1 attB
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attB site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     protein_bind    52..76
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             87..686
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(87..106)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     583..603
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(688..712)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    867..922
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    1352..1377
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    2049..2069
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2114..2132
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2149..2167
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2185..2203
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2221..2239
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2256..2274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2292..2310
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        2322..2360
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2357..2381
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    2400..2424
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4163..4187)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4342..4397
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4758..4774)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4782..4798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4806..4812,4813..4830,4831..4836))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4851..4872
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    4996..5021
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    5693..5713
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        5757..5968
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          5969..6907
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    6932..6956
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6966..7673
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     protein_bind    complement(7676..7700)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7855..7910
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8271..8287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8295..8311
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8319..8325,8326..8343,8344..8349))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8364..8385
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    8509..8534
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    9206..9226
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        9270..9481
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          9482..10420
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     protein_bind    10445..10469
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      10470..10479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10476..11189
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     protein_bind    complement(11193..11217)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    11372..11427
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11788..11804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11812..11828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11836..11842,11843..11860,11861..11866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11881..11902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    12736..13572
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     primer_bind     complement(13683..13702)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             13694..14503
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(13761..13780)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(14756..15344)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14836..14855)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15515..16306,16307..16375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16138..16157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16376..16480)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(16506..16961)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16643..16664)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16855..16874
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     17088..17110
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     17102..17119
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     17103..17119
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17129..17148
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17129..17147
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    17193..17213
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gagggcttgt cgacgacggc ggactccgtc gtcaggatca tgaattcgac cccaagtttg
       61 tacaaaaaag cagacggcct gccaccatga ccgagtacaa gcccacggtg cgcctcgcca
      121 cccgcgacga cgtcccccgg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg
      181 ccacgcgcca caccgtcgac ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac
      241 tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg
      301 cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg
      361 gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc
      421 tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc
      481 ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg
      541 agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg
      601 agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt
      661 gcatgacccg caagcccggt gcctgatacc cagctttctt gtacaaagtg gtacgcgtga
      721 attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg ccaatgccct
      781 ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg acatcatgaa
      841 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
      901 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
      961 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     1021 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     1081 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     1141 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     1201 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     1261 gcccaagctt gaattctcta gaattaccct gttatcccta ggtttaccga gctcttattg
     1321 gttttcaaac ttcattgact gtgccaagct tctgacctct tctcttcctc ccacagggcc
     1381 tcgagacaat tgcatcgatg gtaccagtga aagtctgcaa tgaattcgag ttggcgcgcc
     1441 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
     1501 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     1561 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     1621 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     1681 gggctttggg agggggctgt ccccgtgagc tcccaggcgc gcctgtcatt ctaaatctct
     1741 ctttcagcct aaagcttttt ccccgtatcc ccccaggtgt ctgcaggctc aaagagcagc
     1801 gagaagcgtt cagaggaaag cgatcccgtg ccaccttccc cgtgcccggg ctgtccccgc
     1861 acgctgccgg ctcggggatg cggggggagc gccggaccgg agcggagccc cgggcggctc
     1921 gctgctgccc cctagcgggg gagggacgta attacatccc tgggggcttt gggggggggc
     1981 tgtccccgtg agctcccagg cgcgccaact cggtaccgta tcgatgtcga cgttaacgct
     2041 agtgatatca actttgtata gaaaagttgg ctccgaattc gcccttcagg tccgaggttc
     2101 tagacgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
     2161 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2221 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
     2281 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
     2341 aggcctatat aagcagagct cgtttagtga accgtcagat cgcaaagggc gaattcgacc
     2401 caagtttgta caaaaaagca ggctgatgcc accatgcggg catggactgg ttcctggcgt
     2461 tggattatgc tcattctttt tgcctggggg accttattgt tttatatagg tggtcatttg
     2521 gttcgagata atgaccaccc tgaccattct agcagagaac tctccaagat tcttgcaaag
     2581 ctggagcgct taaaacaaca aaatgaagac ttgaggagaa tggctgagtc tctccgaata
     2641 ccagaaggcc ctattgatca ggggacagct acaggaagag tccgtgtttt agaagaacag
     2701 cttgttaagg ccaaagaaca gattgaaaat tacaagaaac aagctaggaa tgatctggga
     2761 aaggatcatg aaatcttaag gaggaggatt gaaaatggag ctaaagagct ctggtttttt
     2821 ctacaaagtg aattgaagaa attaaagaaa ttagaaggaa acgaactcca aagacatgca
     2881 gatgaaattc ttttggattt aggacatcat gaaaggtcta tcatgacaga tctatactac
     2941 ctcagtcaaa cagatggagc aggtgagtgg cgggaaaaag aagccaaaga tctgacagag
     3001 ctggtccagc ggagaataac atatctgcag aatcccaagg actgcagcaa agccagaaag
     3061 ctggtatgta atatcaacaa aggctgtggc tatggatgtc aactccatca tgtggtttac
     3121 tgcttcatga ttgcttatgg cacccagcga acactcatct tggaatctca gaattggcgc
     3181 tatgctactg gaggatggga gactgtgttt agacctgtaa gtgagacatg cacagacagg
     3241 tctggcctct ccactggaca ctggtcaggt gaagtgaagg acaaaaatgt tcaagtggtc
     3301 gagctcccca ttgtagacag cctccatcct cgtcctcctt acttaccctt ggctgtacca
     3361 gaagaccttg cagatcgact cctgagagtc catggtgatc ctgcagtgtg gtgggtatcc
     3421 cagtttgtca aatacttgat ccgtccacaa ccttggctgg aaagggaaat agaagaaacc
     3481 accaagaagc ttggcttcaa acatccagtt attggagtcc atgtcagacg cactgacaaa
     3541 gtgggaacag aagcagcctt ccatcccatt gaggaataca tggtacacgt tgaagaacat
     3601 tttcagcttc tcgaacgcag aatgaaagtg gataaaaaaa gagtgtatct ggccactgat
     3661 gacccttctt tgttaaagga ggcaaagaca aagtactcca attatgaatt tattagtgat
     3721 aactctattt cttggtcagc tggactacac aaccgataca cagaaaattc acttcggggc
     3781 gtgatcctgg atatacactt tctctcccag gctgacttcc ttgtgtgtac tttttcatcc
     3841 caggtctgta gggttgctta tgaaatcatg caaacactgc atcctgatgc ctctgcaaac
     3901 ttccattctt tagatgacat ctactatttt ggaggccaaa atgcccacaa ccagattgca
     3961 gtttatcctc accaacctcg aactaaagag gaaatcccca tggaacctgg agatatcatt
     4021 ggtgtggctg gaaaccattg gaatggttac tctaaaggtg tcaacagaaa actaggaaaa
     4081 acaggcctgt acccttccta caaagtccga gagaagatag aaacagtcaa ataccctaca
     4141 tatcctgaag ctgaaaaata gtacccagct ttcttgtaca aagtggtacg cgtgaattca
     4201 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc
     4261 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     4321 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     4381 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     4441 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     4501 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     4561 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     4621 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     4681 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     4741 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4801 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4861 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4921 cagcggatcg acagtactaa gcttggtgcg tttttatgct tgtagtattg tataatgttt
     4981 ttaagatcca agcttctgac ctcttctctt cctcccacag ggcctcgaga caattgcatc
     5041 gatggtacca gtgaaagtct gcaatgaatt cgagttggcg cgcctgtcat tctaaatctc
     5101 tctttcagcc taaagctttt tccccgtatc cccccaggtg tctgcaggct caaagagcag
     5161 cgagaagcgt tcagaggaaa gcgatcccgt gccaccttcc ccgtgcccgg gctgtccccg
     5221 cacgctgccg gctcggggat gcggggggag cgccggaccg gagcggagcc ccgggcggct
     5281 cgctgctgcc ccctagcggg ggagggacgt aattacatcc ctgggggctt tgggaggggg
     5341 ctgtccccgt gagctcccag gcgcgcctgt cattctaaat ctctctttca gcctaaagct
     5401 ttttccccgt atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg
     5461 aaagcgatcc cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg
     5521 gatgcggggg gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc
     5581 gggggaggga cgtaattaca tccctggggg ctttgggggg gggctgtccc cgtgagctcc
     5641 caggcgcgcc aactcggtac cgtatcgatg tcgacgttaa cgctagtgat atcaactttg
     5701 tatagaaaag ttggctccga attcgccctt attaattggc tccggtgccc gtcagtgggc
     5761 agagcgcaca tcgcccacag tccccgagaa gttgtgggga ggggtcggca attgaaccgg
     5821 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5881 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5941 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
     6001 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     6061 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     6121 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     6181 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     6241 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     6301 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     6361 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     6421 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     6481 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     6541 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     6601 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     6661 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     6721 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     6781 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     6841 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     6901 atttcaggtg gatcccaagg gcgaattcga cccaagtttg tacaaaaaag caggctgatg
     6961 ccaccatgtc taggctggac aagagcaaag tcataaacgg agctctggaa ttactcaatg
     7021 gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga gttgagcagc
     7081 ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg ccaatcgaga
     7141 tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg caagactttc
     7201 tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac ggggctaaag
     7261 tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat cagctcgcgt
     7321 tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc gccgtgggcc
     7381 actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa gaggaaagag
     7441 agacacctac caccgattct atgcccccac ttctgagaca agcaattgag ctgttcgacc
     7501 ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt ggcctggaga
     7561 aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt gacttagaca
     7621 tgctcccagc cgatgccctt gacgattttg accttgacat gctccccggg taaataccca
     7681 gctttcttgt acaaagtggt acgcgtgaat tcactcctca ggtgcaggct gcctatcaga
     7741 aggtggtggc tggtgtggcc aatgccctgg ctcacaaata ccactgagat ctttttccct
     7801 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
     7861 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     7921 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
     7981 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
     8041 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
     8101 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
     8161 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
     8221 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
     8281 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8341 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8401 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tcgacagtac taagcttggt
     8461 ctaataccca atctctcgtc ttatccagat gttttatacg ccaagcttct gacctcttct
     8521 cttcctccca cagggcctcg agacaattgc atcgatggta ccagtgaaag tctgcaatga
     8581 attcgagttg gcgcgcctgt cattctaaat ctctctttca gcctaaagct ttttccccgt
     8641 atccccccag gtgtctgcag gctcaaagag cagcgagaag cgttcagagg aaagcgatcc
     8701 cgtgccacct tccccgtgcc cgggctgtcc ccgcacgctg ccggctcggg gatgcggggg
     8761 gagcgccgga ccggagcgga gccccgggcg gctcgctgct gccccctagc gggggaggga
     8821 cgtaattaca tccctggggg ctttgggagg gggctgtccc cgtgagctcc caggcgcgcc
     8881 tgtcattcta aatctctctt tcagcctaaa gctttttccc cgtatccccc caggtgtctg
     8941 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     9001 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     9061 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     9121 gggctttggg ggggggctgt ccccgtgagc tcccaggcgc gccaactcgg taccgtatcg
     9181 atgtcgacgt taacgctagt gatatcaact ttgtatagaa aagttggctc cgaattcgcc
     9241 cttattaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     9301 gaagttgtgg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     9361 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     9421 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     9481 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     9541 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     9601 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     9661 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     9721 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     9781 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     9841 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     9901 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     9961 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
    10021 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
    10081 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
    10141 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
    10201 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
    10261 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
    10321 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
    10381 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtggatccca agggcgaatt
    10441 cgacccaagt ttgtacaaaa aagcaggctg ccaccatggt gtctaagggc gaggagctga
    10501 ttaaggagaa catgcacatg aagctgtaca tggagggcac cgtgaacaac caccacttca
    10561 agtgcacatc cgagggcgaa ggcaagccct acgagggcac ccagaccatg agaatcaagg
    10621 tggtcgaggg cggccctctc cccttcgcct tcgacatcct ggctaccagc ttcatgtacg
    10681 gcagcaaaac cttcatcaac cacacccagg gcatccccga cttctttaag cagtccttcc
    10741 ctgagggctt cacatgggag agagtcacca catacgaaga cgggggcgtg ctgaccgcta
    10801 cccaggacac cagcctccag gacggctgcc tcatctacaa cgtcaagatc agaggggtga
    10861 acttcccatc caacggccct gtgatgcaga agaaaacact cggctgggag gcctccaccg
    10921 agatgctgta ccccgctgac ggcggcctgg aaggcagaag cgacatggcc ctgaagctcg
    10981 tgggcggggg ccacctgatc tgcaacttga agaccacata cagatccaag aaacccgcta
    11041 agaacctcaa gatgcccggc gtctactatg tggacagaag actggaaaga atcaaggagg
    11101 ccgacaaaga gacatacgtc gagcagcacg aggtggctgt ggccagatac tgcgacctcc
    11161 ctagcaaact ggggcacaaa cttaattgat aaacccagct ttcttgtaca aagtggtacg
    11221 cgtgaattca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
    11281 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
    11341 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
    11401 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
    11461 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc
    11521 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat
    11581 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg
    11641 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc
    11701 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac
    11761 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    11821 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    11881 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    11941 cctgtcgtgc cagcggatcg acagtactaa gcttggtgaa ttcccttatg tgagtgtaaa
    12001 aggcaggcga gtttgtcccc aattgcatcg atggtaccag tgaaagtctg caatgaattc
    12061 gagttggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
    12121 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
    12181 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
    12241 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
    12301 attacatccc tgggggcttt gggagggggc tgtccccgtg agctcccagg cgcgcctgtc
    12361 attctaaatc tctctttcag cctaaagctt tttccccgta tccccccagg tgtctgcagg
    12421 ctcaaagagc agcgagaagc gttcagagga aagcgatccc gtgccacctt ccccgtgccc
    12481 gggctgtccc cgcacgctgc cggctcgggg atgcgggggg agcgccggac cggagcggag
    12541 ccccgggcgg ctcgctgctg ccccctagcg ggggagggac gtaattacat ccctgggggc
    12601 tttggggggg ggctgtcccc gtgagctccc aggcgcgcca actcggtacc gtatcgatgt
    12661 cgacggtgcg tttttatgct tgtagtattg tataatgttt ttaagatcct agggataaca
    12721 gggtaatcgg ccgctactag ggacaggatt ggtgacagaa aagccccatc cttaggcctc
    12781 ctccttccta gtctcctgat attgggtcta acccccacct cctgttaggc agattcctta
    12841 tctggtgaca cacccccatt tcctggagcc atctctctcc ttgccagaac ctctaaggtt
    12901 tgcttacgat ggagccagag aggatcctgg gagggagagc ttggcagggg gtgggaggga
    12961 agggggggat gcgtgacctg cccggttctc agtggccacc ctgcgctacc ctctcccaga
    13021 acctgagctg ctctgacgcg gctgtctggt gcgtttcact gatcctggtg ctgcagcttc
    13081 cttacacttc ccaagaggag aagcagtttg gaaaaacaaa atcagaataa gttggtcctg
    13141 agttctaact ttggctcttc acctttctag tccccaattt atattgttcc tccgtgcgtc
    13201 agttttacct gtgagataag gccagtagcc agccccgtcc tggcagggct gtggtgagga
    13261 ggggggtgtc cgtgtggaaa actccctttg tgagaatggt gcgtcctagg tgttcaccag
    13321 gtcgtggccg cctctactcc ctttctcttt ctccatcctt ctttccttaa agagtcccca
    13381 gtgctatctg ggacatattc ctccgcccag agcagggtcc cgcttcccta aggccctgct
    13441 ctgggcttct gggtttgagt ccttggcaag cccaggagag gcgctcaggc ttccctgtcc
    13501 cccttcctcg tccaccatct catgcccctg gctctcctgc cccttcccta caggggttcc
    13561 tggctctgct ctccaccgcg ggcagctctg gcccgtgtct caaaatctct gatgttacat
    13621 tgcacaagat aaaataatat catcatgaac aataaaactg tctgcttaca taaacagtaa
    13681 tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat taaattccaa
    13741 catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc
    13801 gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga aacatggcaa
    13861 aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc tgacggaatt
    13921 tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat ggttactcac
    13981 cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg attcaggtga
    14041 aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc ctgtttgtaa
    14101 ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa
    14161 cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg ttgaacaagt
    14221 ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca ctcatggtga
    14281 tttctcactt gataacctta tttttgacga ggggaaatta ataggttgta ttgatgttgg
    14341 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
    14401 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
    14461 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
    14521 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
    14581 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gacggtatca ctagtattac
    14641 agaggtaagt tataacagtc gcctaaccca attgagggga taacgcagga aagaacatgt
    14701 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
    14761 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
    14821 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
    14881 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
    14941 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
    15001 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
    15061 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
    15121 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
    15181 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
    15241 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
    15301 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
    15361 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
    15421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
    15481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
    15541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
    15601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
    15661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
    15721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
    15781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
    15841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    15901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    15961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    16021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    16081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    16141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    16201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    16261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    16321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    16381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
    16441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
    16501 cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
    16561 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
    16621 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
    16681 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
    16741 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
    16801 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
    16861 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
    16921 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc cattcaggct
    16981 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
    17041 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
    17101 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
    17161 cgtatcgatg tcgacgttaa cgctagtgat atcaactttg tatagaaaag ttggctccga
    17221 atttctc
//
