LOCUS       sequence-239218-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSKAP plasmid containing the unique DNA barcode ""AE"" to be used 
            for allelic exchange on bacterial chromosomes and subsequent 
            molecular identification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Shaw JA, Bourret TJ
  TITLE     Digital PCR-based Competitive Index for High-throughput Analysis of 
            Fitness in Salmonella.
  JOURNAL   J Vis Exp. 2019 May 13;(147). doi: 10.3791/59630.
  PUBMED    31132072
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2019-05-13"; volume: "(147). doi"; pages: " 
            10.3791/59630"
COMMENT     Sequence Label: pSKAP_AE
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..49
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     34..50
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(140..187)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(311..330)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 tmttmtatag gcgattgggt acgggccccc cctcgaggtc gacggtatcg ataagcttgc
       61 atgcagattg cagcattaca cgtcttgaga tgaggacgga gacgtgattg tagccgattg
      121 tgtaggctgg agctgcttcg aagttcctat actttctaga gaataggaac ttcggaatag
      181 gaacttcaag atccccttat tagaagaact cgtcaagaag gcgatagaag gcgatgcgct
      241 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      301 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      361 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      421 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
      481 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      541 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
      601 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      661 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      721 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      781 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcacggca ccggacaggt
      841 cggtcttgac aaaaagaacc gggcgcccct gcgct
//
