LOCUS       sequence-239265-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSKAP plasmid containing the unique DNA barcode ""AT"" to be used 
            for allelic exchange on bacterial chromosomes and subsequent 
            molecular identification..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Shaw JA, Bourret TJ
  TITLE     Digital PCR-based Competitive Index for High-throughput Analysis of 
            Fitness in Salmonella.
  JOURNAL   J Vis Exp. 2019 May 13;(147). doi: 10.3791/59630.
  PUBMED    31132072
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2019-05-13"; volume: "(147). doi"; pages: " 
            10.3791/59630"
COMMENT     Sequence Label: pSKAP_AT
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     36..52
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(142..189)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(313..332)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 amymcwwwrr gggsgatttg ggtmcgggcc cccctcgagg tcgacggtat cgataagctt
       61 gcatgcagat tgcagcatta cacgtcttga gaccagtgtc cgtgacatgg ctagaccgat
      121 tgtgtaggct ggagctgctt cgaagttcct atactttcta gagaatagga acttcggaat
      181 aggaacttca agatcccctt attagaagaa ctcgtcaaga aggcgataga aggcgatgcg
      241 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
      301 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
      361 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
      421 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
      481 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
      541 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
      601 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
      661 tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag
      721 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
      781 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggc accggacagt
      841 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggacacgg cggcatcaga
      901 gcagcgattg gtctgtgtgc cagtcatagc cgattagcct yctccaccca agcgtcgggr
      961 araccttg
//
