LOCUS       sequence-239285-        3230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Storz P, Toker A
  TITLE     Protein kinase D mediates a stress-induced NF-kappaB activation and 
            survival pathway.
  JOURNAL   EMBO J. 2003 Jan 2. 22(1):109-20.
  PUBMED    12505989
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            2003 Jan 2. 22(1):109-20."
COMMENT     Sequence Label: pSUPER.PKD1.RNAi
FEATURES             Location/Qualifiers
     source          1..3230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        89..303
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     224..243
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(376..392)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(377..393)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(421..439)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(460..476)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(460..476)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(473..495)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    484..500
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(508..514,515..532,533..538))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    553..574
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(691..708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(862..1450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(942..961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1621..2412,2413..2481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2244..2263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2482..2586)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2612..3067)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2749..2770)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2961..2980
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3194..3216
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3208..3225
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3209..3225
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtggatcc cccgggctgc aggaattcga acgctgacgt catcaacccg ctccaaggaa
      121 tcgcgggccc agtgtcacta ggcgggaaca cccagcgcgc gtgcgccctg gcaggaagat
      181 ggctgtgagg gacaggggag tggcgccctg caatatttgc atgtcgctat gtgttctggg
      241 aaatcaccat aaacgtgaaa tgtctttgga tttgggaatc ttataagttc tgtatgagac
      301 cacagatccc catgctgtgg gggctggtac ttcaagagag taccagcccc cacagcattt
      361 tttggaaaag cttatcgata ccgtcgacct cgaggggggg cccggtacca gcttttgttc
      421 cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
      481 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      541 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      601 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
      661 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
      721 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
      781 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
      841 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
      901 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
      961 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1021 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1081 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1141 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1201 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1261 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     1321 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     1381 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     1441 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     1501 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     1561 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     1621 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     1681 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     1741 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     1801 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     1861 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     1921 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     1981 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2041 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2101 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2161 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2221 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     2281 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     2341 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     2401 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     2461 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     2521 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     2581 tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa
     2641 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2701 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2761 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2821 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2881 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2941 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     3001 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     3061 ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
     3121 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
     3181 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
//
