LOCUS       sequence-239299-        2844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty vector to clone and measure the expression of promoter 
            candidates (spike-in) by determining the abundance of transcripts 
            originating from the candidate in human cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pSTAP-seq_human_spike-in
FEATURES             Location/Qualifiers
     source          1..2844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          126..258
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     241..260
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    382..503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(393..412)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     447..466
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(767..784)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(938..1526)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1018..1037)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1726..2517,2518..2586))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    2691..2739
                     /note="synthetic polyadenylation signal"
     misc_feature    2753..2844
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2793..2812
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtaccg agctcttacg cgtgctagcc cgggctcgag atcttagagc
       61 atgcaccggt gatatcgata tcgtcgacga attcggccat ggcccggtcg tgaggcactg
      121 ggcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaatagaaa ctgggcttgt
      181 cgagacagag aagactcttg cgtttctgat aggcacctat tggtcttact gacatccact
      241 ttgcctttct ctccacaggt gtccaccctg caggcttaag catggctagc aaaggagaag
      301 aactcttcac tggagttgtc ccaattcttg ttgaattaga ttgatctaga gaattcggcc
      361 ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
      421 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      481 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      541 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg
      601 ataaggatcc gaccgatgcc ctcaagcttt gagaggggcc ctgagagcct tcaacccagt
      661 cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt
      721 tatcatgcaa ctcgtaggac aggtgccggc agcgctcttc cgcttcctcg ctcactgact
      781 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
      841 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
      901 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      961 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1021 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1081 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1141 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1201 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1261 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1321 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     1381 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     1441 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     1501 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     1561 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     1621 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     1681 aaacttggtc tgacagcggc cgcaaatgct aaaccactgc agtggttacc agtgcttgat
     1741 cagtgaggca ccgatctcag cgatctgcct atttcgttcg tccatagtgg cctgactccc
     1801 cgtcgtgtag atcactacga ttcgtgaggg cttaccatca ggccccagcg cagcaatgat
     1861 gccgcgagag ccgcgttcac cggcccccga tttgtcagca atgaaccagc cagcagggag
     1921 ggccgagcga agaagtggtc ctgctacttt gtccgcctcc atccagtcta tgagctgctg
     1981 tcgtgatgct agagtaagaa gttcgccagt gagtagtttc cgaagagttg tggccattgc
     2041 tactggcatc gtggtatcac gctcgtcgtt cggtatggct tcgttcaact ctggttccca
     2101 gcggtcaagc cgggtcacat gatcacccat attatgaaga aatgcagtca gctccttagg
     2161 gcctccgatc gttgtcagaa gtaagttggc cgcggtgttg tcgctcatgg taatggcagc
     2221 actacacaat tctcttaccg tcatgccatc cgtaagatgc ttttccgtga ccggcgagta
     2281 ctcaaccaag tcgttttgtg agtagtgtat acggcgacca agctgctctt gcccggcgtc
     2341 tatacgggac aacaccgcgc cacatagcag tactttgaaa gtgctcatca tcgggaatcg
     2401 ttcttcgggg cggaaagact caaggatctt gccgctattg agatccagtt cgatatagcc
     2461 cactcttgca cccagttgat cttcagcatc ttttactttc accagcgttt cggggtgtgc
     2521 aaaaacaggc aagcaaaatg ccgcaaagaa gggaatgagt gcgacacgaa aatgttggat
     2581 gctcatactc gtcctttttc aatattattg aagcatttat cagggttact agtacgtctc
     2641 tcaaggataa gtaagtaata ttaaggtacg ggaggtattg gacaggccgc aataaaatat
     2701 ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta ctaacatacg
     2761 ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc cccagtgcaa
     2821 gtgcaggtgc cagaacattt ctct
//
