LOCUS       sequence-239301-        5122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  STAP-seq luciferase validation vector, which harbors an 4x UAS array
            upstream of the CP position (candidates are inserted at CP position)
            for the recruitement of GAL4-DBD tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pGL3_4xUAS_CP-candidate_luc+
FEATURES             Location/Qualifiers
     source          1..5122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    39..160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(50..69)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     104..123
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(408..425)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(579..1167)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(659..678)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1338..2129,2130..2198))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1961..1980
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2199..2303)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      2330..2785
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2417..2436)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2627..2648
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    2916..2964
                     /note="synthetic polyadenylation signal"
     misc_feature    2978..3069
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     3018..3037
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     protein_bind    3110..3134
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3227..3251)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3462..3471
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3468..5120
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3534..3552)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg
       61 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
      121 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
      181 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
      241 caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag
      301 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
      361 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
      421 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      481 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      541 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      601 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      661 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
      721 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
      781 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
      841 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      901 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
      961 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     1021 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1081 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1141 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1201 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1261 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1321 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1381 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1441 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1501 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1561 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1621 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1681 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     1741 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     1801 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     1861 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     1921 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     1981 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2041 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2101 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2161 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2221 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2281 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgcgccctgt
     2341 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     2401 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     2461 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     2521 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     2581 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     2641 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     2701 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     2761 aacaaaatat taacgcttac aatttgccat tcgccattca ggctgcgcaa ctgttgggaa
     2821 gggcgatcgg tgcgggcctc ttcgctatta cgccagccca agctaccatg ataagtaagt
     2881 aatattaagg tacgggaggt acttggagcg gccgcaataa aatatcttta ttttcattac
     2941 atctgtgtgt tggttttttg tgtgaatcga tagtactaac atacgctctc catcaaaaca
     3001 aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag tgcaagtgca ggtgccagaa
     3061 catttctcta tcgataggta cctgagctcg ctagcctcga ggatatcaaa caagtttgta
     3121 caaaaaagca ggcttcgtcg gagtactgtc ctccgagcgg agtactgtcc tccgaggccg
     3181 ccgcccggag tactgtcctc cgagcggagt actgtcctcc gaggtgaccc agctttcttg
     3241 tacaaagtgg tatatcaaga tctgcgatcg agctcgcccg gggatcgagc gcagcggtat
     3301 aaaagggcgc ggggtggctg agagcatcag ttgtgaatga atgttcgagc cgagcagacg
     3361 tgccgctgcc ttcgttaata tcctttgaat aagccaactt tgaatcacaa gacgcatacc
     3421 aaaccctgca ggaagcttgg cattccggta ctgttggtaa agccaccatg gaagacgcca
     3481 aaaacataaa gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc
     3541 aactgcataa ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg
     3601 cacatatcga ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag
     3661 aagctatgaa acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact
     3721 ctcttcaatt ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg
     3781 cgaacgacat ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg
     3841 tggtgttcgt ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa
     3901 tcatccaaaa aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt
     3961 acacgttcgt cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt
     4021 ccttcgatag ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc
     4081 ctaaaggtgt cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc
     4141 ctatttttgg caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc
     4201 acggttttgg aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa
     4261 tgtatagatt tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg
     4321 cgctgctggt gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg
     4381 atttatctaa tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg
     4441 aagcggttgc caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga
     4501 ctacatcagc tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag
     4561 ttgttccatt ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta
     4621 atcaaagagg cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc
     4681 cggaagcgac caacgccttg attgacaagg atggatggct acattctgga gacatagctt
     4741 actgggacga agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca
     4801 aaggctatca ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct
     4861 tcgacgcagg tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg
     4921 ttgttttgga gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc
     4981 aagtaacaac cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag
     5041 gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg
     5101 gcggaaagat cgccgtgtaa tt
//
