LOCUS       sequence-239303-        6516 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pENTR_hEMSY
FEATURES             Location/Qualifiers
     source          1..6516
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             950..961
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      3744..3753
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(4641..4740)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(4757..4776)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4758..4776)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4781..4797)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4781..4797)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4899..4918)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4910..5719
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4977..4996)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      5866..6454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6355..6374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc atgcctgttg tgtggccaac ccttctggat ctcagcaggg atgaatgcaa
      721 aagaattctt cgaaaattgg aattggaggc atatgctgga gttatcagtg cacttcgggc
      781 acagggggat ctcaccaagg aaaagaaaga tcttcttgga gaactatcaa aagttcttag
      841 catctcaaca gaacgccacc gtgctgaagt tcggagagca gtaaacgatg aacggttaac
      901 aacaattgca cataatatgt ctggacctaa tagctcttca gaatggtcca ttgaaggtcg
      961 tcgattggta ccactgatgc cccggctcgt tccccaaacc gcctttactg taacagctaa
     1021 tgctgttgct aatgcagcta tccagcataa tgcatctctt ccagtgcctg cagaaacagg
     1081 aagcaaggaa gtagtggttt gctattccta cacaagtacc acgtcaaccc caacctctac
     1141 ccctgttcca agtggcagca tagcaacggt taagtctcca agacctgcca gtcctgcctc
     1201 caatgtagtt gtcttgccaa gtggaagtac tgtttatgtc aaaagtgtaa gctgttcaga
     1261 tgaagatgaa aaacccagaa aacgaaggcg aacaaactct tccagctcct ctcctgttgt
     1321 tctaaaggaa gttccaaagg ccgttgttcc agtctcaaag acgatcactg tgcctgtgag
     1381 tggtagtccc aagatgagca acatcatgca gagcattgcc aactccttac caccccacat
     1441 gtctcctgta aaaataacct tcactaaacc atcaacacag acaacaaaca caacaacaca
     1501 gaaggttatt atagtcacca catcaccaag ctcaaccttc gtgcccaaca ttctctccaa
     1561 atcccataac tatgcagcag tcactaagct tgtaccaacg tcagtcattg cttctacaac
     1621 ccagaagcca ccagttgtta taactgcttc acagtcctct ctggtcagta atagcagcag
     1681 tggcagcagc agttctacac catcacctat tcctaataca gttgcagtaa cagctgtggt
     1741 gtcctctaca ccatctgtgg tcatgtcaac agtagcacaa ggtgtatcta catcagcaat
     1801 caaaatggca tcaaccagac ttccttcccc caaaagctta gtgagtgccc caactcagat
     1861 tcttgcacag ttccctaaac aacatcaaca gtctcctaag cagcagttat atcaagtgca
     1921 acagcagaca cagcaacaag tggcccagcc ttctccagta tctcatcagc aacagcctca
     1981 gcagtctcct ttgccacctg gtattaaacc taccatccaa atcaaacagg agtcaggtgt
     2041 taaaatcatc acacaacagg ttcaaccaag taaaatctta cccaaaccag tgacagcaac
     2101 tctacccacc agtagcaatt cccctattat ggtggttagc agtaatggtg caattatgac
     2161 aactaaactg gtaaccactc ctactggcac acaagcaacc tatacccggc caacagtgag
     2221 cccatccatt ggtcggatgg ctgcaacccc tggagctgca acctatgtga aaactacgag
     2281 tggtagcatc attacagtag tacccaaatc attagctacc ttggggggca agataattag
     2341 cagtaatata gtttctggaa cgactaccaa aatcactaca atcccaatga cttccaagcc
     2401 caacgtgatt gttgtacaaa agactacagg aaaaggaacg accattcaag gcctcccggg
     2461 caaaaatgtt gtcacaacgt tgctaaatgc tggaggagaa aagactattc agacagtgcc
     2521 aacaggagca aagccagcta tccttactgc tacaagaccc atcaccaaaa tgattgtaac
     2581 gcagccaaaa ggaataggtt ctacagttca accagcagct aaaatcatcc caacaaaaat
     2641 tgtttatggg cagcaaggga aaacgcaggt tcttattaaa cccaaaccag tgacttttca
     2701 agcgacagtt gttagtgaac aaacaagaca gctagtaaca gaaacattac agcaagcatc
     2761 cagggtagca gaggctggta attcatctat tcaggaagga aaagaagaac cacagaatta
     2821 tacagatagt agttcctctt ctacagagtc ctcccagagt tcccaagatt cccagcctgt
     2881 agttcatgta attgcttccc ggcgtcagga ttggtcagaa catgagattg caatggagac
     2941 tagccctacc ataatttatc aggatgtatc cagtgaatca caatcagcta cttcaacaat
     3001 caaagctctg ttagaactcc aacagacaac agtaaaggaa aaattggaat ctaaaccaag
     3061 acaacccact attgacctga gtcaaatggc agtgcctatt cagatgaccc aggaaaagag
     3121 acattctcct gagagtccat caattgctgt ggtagagtca gaactagtag ctgaatacat
     3181 cactactgaa cgcactgatg aggggacaga ggttgctttt ccccttctag tcagccatcg
     3241 ctcccagccc caacagcctt cccagcccca gcggaccctg ctccagcatg tggctcagtc
     3301 acagaccgca acacagactt cggtggtggt gaagtccatc ccagcatctt cccctggagc
     3361 aatcacccac attatgcagc aggcattaag cagtcacact gcttttacca aacacagcga
     3421 ggaacttgga actgaggagg gcgaggttga agagatggac actttagacc ctcagacagg
     3481 tctgttttac cgatctgccc tgactcagtc acagtcagct aaacagcaga aacttagcca
     3541 gcccccgctg gaacagactc agctgcaagt gaaaactctg cagtgcttcc agactaaaca
     3601 gaagcagacc atccacctgc aggcagacca gctccagcac aaactcccgc aaatgcccca
     3661 gctttccatc aggcatcaaa aactcacccc tctccagcaa gaacaagcac agcccaagcc
     3721 agatgtacag cacacacagc atcccatggt ggccaaagac aggcagcttc ctaccttaat
     3781 ggcacagccc ccgcaaactg tagtacaggt gcttgcagtg aaaaccacgc agcagctccc
     3841 taaactgcag caggctccga accaaccaaa aatctacgtg caaccccaaa ccccccagag
     3901 ccaaatgtcg ctcccagctt cttcagagaa acagacggca agccaggtgg agcagccaat
     3961 tataacccaa ggatcctctg ttacaaagat aacttttgag gggcgccagc ctcccacagt
     4021 tacaaagata actggtggca gttctgtgcc taagctgaca tcaccagtta caagcatatc
     4081 tcccattcag gcctctgaga agacagcagt gtctgacatt ttgaaaatgt ctttgatgga
     4141 agctcagatt gatacaaatg tagaacatat gatagtggat cccccaaaga aggctcttgc
     4201 cactagcatg ctcactggtg aagcaggatc attaccctcc acccacatgg tggtggcagg
     4261 gatggcgaat tccactcccc agcaacagaa atgtagagag tcctgttcga gtccatccac
     4321 tgttggctct tccctaacga caaggaaaat tgatccacca gcagtgcctg cgacaggcca
     4381 gttcatgcgt attcagaatg taggccaaaa gaaagctgaa gagagtccag cagaaattat
     4441 catccaggct attcctcagt atgctattcc ttgtcactcc agctccaatg tggtggtgga
     4501 gcccagtggg cttcttgagc taaacaactt cactagtcaa cagctggatg atgaggagac
     4561 agcaatggag caggacatag acagtagcac ggaggatgga actgaaccca gcccttctca
     4621 gagctctgct gaacggtccg acccagcttt cttgtacaaa gttggcatta taagaaagca
     4681 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg
     4741 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca
     4801 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa taatatcatc
     4861 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat
     4921 tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta
     4981 taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa
     5041 gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac
     5101 agatgagatg gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca
     5161 ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc
     5221 attccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt
     5281 gttcctgcgc cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt
     5341 atttcgtctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt
     5401 tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt
     5461 gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt
     5521 tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata
     5581 ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg
     5641 gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat
     5701 gctcgatgag tttttctaat cagaattggt taattggttg taacactggc agagcattac
     5761 gctgacttga cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc
     5821 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     5881 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     5941 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     6001 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     6061 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     6121 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     6181 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     6241 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     6301 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     6361 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     6421 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     6481 gttcctggcc ttttgctggc cttttgctca catgtt
//
