LOCUS       sequence-239306-        4443 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Haberle V, Arnold CD, Pagani M, Rath M, Schernhuber K, Stark A
  TITLE     Transcriptional cofactors display specificity for distinct types of 
            core promoters.
  JOURNAL   Nature. 2019 Jun;570(7759):122-126. doi: 10.1038/s41586-019-1210-7. 
            Epub 2019 May 15.
  PUBMED    31092928
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41586-019-1210-7"; journalName: "Nature"; date: "2019-06";
            volume: "570"; issue: "7759"; pages: "122-126"
COMMENT     Sequence Label: pENTR_Trf2
FEATURES             Location/Qualifiers
     source          1..4443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2568..2667)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2684..2703)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2685..2703)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2708..2724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2708..2724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2826..2845)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2837..3646
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2904..2923)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3793..4381
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4282..4301
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacacattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggcttc caaaacgata tggtcagcat accggtggcc aatttgaacg gcggccttaa
      721 agcggccagc agcggttccg gagttggtgt agtgacctcc ggcggcgtcg tatcctcggc
      781 ggtgctggcc aatgccccac gtgtatacct aacgccctcc tccacattca tgaccaatcg
      841 ccaaatggcg ggagtggcgt ccaccggaag aatgagcgga caagtcgtcg gtggttcatc
      901 tggcacagcc tcaacagccg gtaccgttcg ctatttttcg caattcagca aaatgcaaac
      961 ggccggcgga cccagtttgc aaaggaaact tgccaacggg gataccattg ttctggcaac
     1021 cggcagtaaa aatatgttcc ttactagcag cgaaaataag gctaatctgc cgaccgtcgc
     1081 cagcaatggc aatggtctga tcacagccaa aatggatcta ttggaggagg aggtcatgca
     1141 gtcgataact gtgattgatg acgatgacga ggagaaaaag gaagtcgctg aagacgaaga
     1201 agagagcagc aataatgcca aacccattga ccttcaccag ccaattgctg ataatgagca
     1261 cgagctggac attgtgataa acaatgtggt ctgctcgttt agcgtcggct gtcacctgaa
     1321 gctgcgcgaa attgcgctgc agggatcaaa tgtggagtac cgtcgcgaga acggaatggt
     1381 gacaatgaag ctccgccatc catacaccac tgcctcgatt tggtcgtcgg ggaggattac
     1441 ctgcactggt gccacttccg agtcaatggc caaggtcgct gcacgccgct atgccagatg
     1501 ccttggcaag ctgggatttc ccactcgttt cctcaacttt cgcattgtca acgtactggg
     1561 cacctgcagc atgccgtggg ccatcaagat cgtcaacttt tcggagcgcc atcgcgagaa
     1621 cgccagctac gagcccgagc tgcatcctgg agtgacttac aagatgcgcg atcccgatcc
     1681 aaaggccacc ctgaagatct tctccaccgg cagcgtaaca gtcaccgcgg ctagcgtaaa
     1741 ccacgttgaa tcggctatcc agcacatata cccgctggtc tttgatttcc gcaagcaacg
     1801 ttcggcggag gagctgcagc atctgcgtca aaagcagcgt ctgcaagccg gcggtgatcc
     1861 ccacgaactg gagaaaaatg tcttggccga taacaagact gcttcccttg ataatatctt
     1921 tgttaataca acagcggcgc actctaaatc atcgtcgaat gaccaaacat cagcacccgc
     1981 gaccatcttg tccagcacag ttgatagtat gcctcgtctg aaacagatgg tcaactatca
     2041 ccagatgatg aagcaaaccc aggaagagcg ccgccatata atgtttaatg gggagaaggc
     2101 caatccggcc tccacctcat cggcggcagc agctccctcc acctcctcct catcgtcctc
     2161 atccggggat aatatctgtg ccaatgcccg tcgccgggcc accgaatgct gggcaaccaa
     2221 gctgcagaat aagcgtccac gttacaatga tcccggtacc acgggcacaa ttaacgccgc
     2281 atcgtccaca gcatcagccg ccacatcttc gttagcctcc caggcgacac atctgcgtaa
     2341 tcctctcaag acggccgcgt tggccaatgc acgtatgtta ggcgccaagg tgacaacatg
     2401 cacacgcaac agcataatcg tgcagcagcc gcaacgtatc caaatgcagc agcagcagca
     2461 acagctgcaa ccgcagcagc aacagaccag cttctcgccc agcgaattcg atgttgatga
     2521 cttgatcgag gaggaggaga acaacgaatt ggatatgccc ttctaggacc cagctttctt
     2581 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2641 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2701 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2761 cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2821 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2881 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2941 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     3001 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     3061 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     3121 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     3181 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     3241 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     3301 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3361 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3421 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3481 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3541 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3601 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3661 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3721 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3781 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3841 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3901 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3961 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     4021 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     4081 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     4141 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     4201 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     4261 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     4321 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4381 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4441 gtt
//
