LOCUS       sequence-239311-        2858 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for LuxRQ-FMY (Adam Meyer's 
            improved LuxR activator from https://doi.org/10.1101/285866, 
            selected for dynamic range and low cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2858)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C74m
FEATURES             Location/Qualifiers
     source          1..2858
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(357..1148,1149..1217))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     980..999
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1218..1322)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1390..1408
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(1412..1455)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    1458..1479
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             2243..2254
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    2259..2279
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(2285..2302)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2456..2858,1..186))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2536..2555)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
       61 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
      121 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
      181 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
      241 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
      301 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
      361 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
      421 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
      481 gctgcaatga taccgcgcga cccacgctca ccggctccag atttatcagc aataaaccag
      541 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
      601 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
      661 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
      721 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
      781 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
      841 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
      901 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
      961 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     1021 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     1081 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     1141 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     1201 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     1261 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     1321 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta
     1381 acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc
     1441 gctaaggatg atttctggaa ttcgcggccg cttctagaga ctagtgggtc tcaaatgaaa
     1501 aacataaatg ccgacgacac atacagaata attaataaaa ttaaagcttt tagaagcaat
     1561 aatgatatta atcaatgctt atctgatatg actaaaatgg tacattgtga atattattta
     1621 ctcgcgatca tttatcctca ttctatggtt aaatctgata tttcaatcct agataattac
     1681 cctaaaaaat ggaggcaata ttatgatgac gctaatttaa taaaatatga tcctatagta
     1741 gattattcta actccaatca ttcaccaatt aattggaata tatttgaaaa caatgctgta
     1801 aataaaaaat ctccaaatgt aattaaagaa gcgaaaacat caggtcttat cactgggttt
     1861 agtttcccta ttcatatggc taacaatggc ttcggaatgc ttagttttgc atattcagaa
     1921 aaagacaact atatagatag tttattttta catgcgtgta tgaacatacc attaattgtt
     1981 ccttctctag ttgataatta tcgaaaaata aatatagcaa ataataaatc aaacaacgat
     2041 ttaaccaaaa gagaaaaaga atgtttagcg tgggcatgcg aaggaaaaag ctcttgggat
     2101 atttcaaaaa tattaggatg cagtgagcgt actgtcactt tccatttaac caatgcgcaa
     2161 atgaaactca atacaacaaa ccgctgccaa agtatttcta aagcaatttt aacaggagca
     2221 attgattgcc catactttaa aaattgaagg tagagaccta ctagtagcgg ccgctgcagg
     2281 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     2341 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2401 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2461 acaggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2521 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     2581 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     2641 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     2701 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2761 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2821 ggattagcag agcgaggtat gtaggcggtg ctacagag
//
