LOCUS       sequence-23932-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of mAmetrine (version 1.1) fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: pBad-mAmetrine1.1
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             85..102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             106..138
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(142..150,151..165)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(226..247)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctactgagct tttttcgcaa ctctctactg tttctccata cccgtttttt gggctaacag
       61 gaggaattaa ccatgggggg ttctcatcat catcatcatc atggtatggc tagcatgact
      121 ggtggacagc aaatgggtcg ggatctgtac gacgatgacg ataaggatcc gagctcgagc
      181 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
      241 ggcgacgtaa acggccacag gttcagcgtg cgcggcgagg gcgagggcga tgccaccaac
      301 ggcaagctga ccctgaagtt catctgcacc tccggcgagc tgcccgtgcc ctggcccacc
      361 ctcgtgacca ccctgtctta cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
      421 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatctcc
      481 ttcaaggacg acggcagcta caggacccgc gccgaggtga agttcgaggg cgacaccctg
      541 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      601 aagctggagt acaacatgaa cgcgtgggac gcgtatatca cggccgacaa gcagaagaac
      661 ggcatcaaag cgaacttcaa gatcgaccat anggggnngg nnngnggcct ggcagctcgc
      721 cgacgcgtac cagcagaaca ccccatcggc gacggctcgt gctgctgctg acaccactac
      781 ctgactncag acagtgtcaa aaccnaagac acgcgatact gtc
//
