LOCUS       sequence-239320-       14078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pmbfA::cexA::ttrpC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Steiger MG, Rassinger A, Mattanovich D, Sauer M
  TITLE     Engineering of the citrate exporter protein enables high citric acid
            production in Aspergillus niger.
  JOURNAL   Metab Eng. 2019 Mar;52:224-231. doi: 10.1016/j.ymben.2018.12.004. 
            Epub 2018 Dec 13.
  PUBMED    30553933
REFERENCE   2  (bases 1 to 14078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2019-03"; volume: "52:224-231. doi"; pages: " 
            10.1016/j.ymben.2018.12.004. Epub 2018 Dec 13"
COMMENT     Sequence Label: pMST-712
FEATURES             Location/Qualifiers
     source          1..14078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      517..526
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     terminator      complement(5573..6346)
                     /gene="trpC"
                     /label=trpC terminator
                     /note="transcription terminator from the Aspergillus 
                     nidulans trpC gene (Punt et al., 1987)"
     primer_bind     complement(10602..10619)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10602..10618)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10659..11246
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11147..11166
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(11258..11285)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(11309..12124)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCLKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNGDRVFRLAQAQSRMNNGLVGA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12032..12051
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(12125..12227)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     terminator      complement(12228..12259)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     primer_bind     12307..12323
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12307..12323
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(12986..14011)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(12986..14011)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
ORIGIN
        1 cgggaggggg ggagagaaat cagcaggtga ggctcacgag tcaggtcatc gtcaagctcc
       61 cgtgagtgaa aaaagagacg acgataaggc gatgcgtgca aaagagatgc agacaaaggg
      121 gcagatggac caaaggcaga caaggatgga gagggaaata aataagggaa caagggcggc
      181 ttcgacgagg gagagagaaa aaaaaaacaa cctgagctgc gggaaggcga gccaagatta
      241 ggtgatgctg cgcgctgagt gtgcaggtgg tctggggtgg agggaggggg gtggcagagc
      301 cggcagccgt cagtggttgg attttttttt ttttttttct cacttcaacc tcaccttttt
      361 tcccctctct ctctctctct ctctctcctc tctctatact gtacctagca ataatagctc
      421 atggactcac agcaacgctc tgtgagccaa ccaagggccc ggattggcca cagcagccac
      481 gacaaagcac ggcatccctc cgttggttcg ctgctcccat ggcggccgct ttgctccaac
      541 tcaggcgatg ctgcccctcc atccttgccc tcgcctcgcc agtgaggggc attgagttga
      601 gcgctaactg aggggcaggc caacgtggaa cagctgggat aaggggaccg gccgctagtc
      661 tcaccgttat tcatgaatta gcgccactgg actctcaggc tttccattgg ggtactaaca
      721 tagccatcaa atgccggccc gtgtttaatc tcctcgagat ggctgggctc acatgcgttc
      781 tactcaaaaa ctcttatacc taggccctct cccaatcgtg tatgtttgga ccttcctctc
      841 tcacactttg gataacatca agcatactgc agattttgac ctagttaaca aggcacatca
      901 tgctatcact tccaacactc ttgctgagcg cgttctgcct tgcctttctg gttctacgaa
      961 gtatccagtc ccgcctgcaa cgccgcagaa acgccaaaag gtggggttgt caaccgccag
     1021 ctagagctcc atcgggattc tttggcattc cgccgttcct cggtggtcgc agataactcc
     1081 ctaaattccc tgagctaact ctaagtcgac catgccgttt atggttagcg cctcccaaaa
     1141 aggaatggcc gacttagagt tacctcttga ccgacttttt ctttcctccc ccttacattt
     1201 cgttaccaca acacattcct atatccaaac tccaggtaca taactagtcg aaatctcttt
     1261 taaatctagt caagaactag atacttaacc ttcatactgc ttagagatag cctttgaagc
     1321 tcttatttcg ctctttgtct cactctcacc ttcctcctcc tcctccaatc ctttttgctc
     1381 gggactagtc caagactagt caacgattag tatgccgagc attcgcgata acgatctccg
     1441 aaagtcccca gagtactgtc actatctcga ggcagttaag gacggggagc ttacgctgcc
     1501 ggatttcaag atagtaagcc gacccgacta gctttaagcc tagttactga ctagtccaca
     1561 ggacgacaat ggcgtgcctg atatccatcc atatgaagtc tactgccgag tgaagggatg
     1621 cctcaagcgt acagtgagtc tactgctata ctagtttctg actagttatt aactagttga
     1681 caggttccct ctgccaacag aaacatattg gtcaagcact tgaaggacaa gaactcccac
     1741 ggcatggagt ttacattgca caatggacct cccactatga aggaactgat ggaggccaaa
     1801 ggcaagtcct atctagatta cttggtgact agtccctgac tagtcaagta gcatggtatg
     1861 aaggcttgtt tgaaggcact gttctcccaa ccccgactcc taccaagaag cgcaagcgag
     1921 ctgcgtaagt ttctgtgagt ctaactagtg tattagctaa tatatagcag caccaagtcc
     1981 aaggaccaca atactaaggg tgtcgagaat tcgtgagttt cttctcccat ttcaactagt
     2041 ccttgactag tcactaacta ctttgcagca acgagggtga agctggaaat gatcaggaca
     2101 atggcgaggg cccgtaagta cagccattca atgcagacta gttgctaact aatctgtgac
     2161 tagttcaagt ggtccgtacg ccgtgcatac ccctgtgact ggtaggaatt tgagcaagcc
     2221 tgtcttgccg cgcgatgaga aaggaaaggc aagttacatt cagccccgta cctaggatca
     2281 gtgctaattt ataacctcta gccactcttt atgcagatcc gccgtgaggg tagcaaggca
     2341 gctaaatcag ctggtgagaa aggaaccata ccctgcaaga cctgtcgcaa cgcaaagggc
     2401 aaaggtaagc tatccaagct agtttgggac tagattctaa ctagtctcag caccgtgtgg
     2461 ttcaaagcca tattgcgagt tttggcgctt tttctcatcg attgacgagg caaagggagc
     2521 gagtatgcaa cctcgtaagt cagactcaga caaagaccaa ctagttattg accagtcatc
     2581 tagaaggctc tgttgtggat cttgaggccc tggagagttc ctccaacaat ccggagacaa
     2641 gcaagtcctc gtcggactag tcactaacta gactctaact agttgcagac atggataatg
     2701 caaaagagac aagcaatgaa gaaagtggta agacatttct cctttgtggt tctggactag
     2761 tctttgacta gtcacagtct taaacaagga aaatgagcat gaaaatgagg aggaaaaggc
     2821 tgctgagccc gaggaagtgc agggtgatgg cagacatggt aggttaatac cttgttagtt
     2881 attgctagtc actgactagt caataactag tctctgaaca ccttgcaatc actccgtttg
     2941 cgcagctgaa cagtggtgag gataatagta gtaagttatt ctagcttcag agttatagga
     3001 gactagatac taactagtat tagttgcaac taacctggat ctcagagact ttggcctcaa
     3061 tctagaatct atctagttgt caactagact gtggtatcat tgtcttttat tttcctagtc
     3121 ctggaactag cttctaacta gtctccctaa tatgtggctg tcttgttttt tttttttgtt
     3181 tccctacccg gatatctagt ccccttctag gttctgttaa cctctcgggc tctgatttag
     3241 tttaacgcaa acctgagatt agtttctaac tagtctctag gttttctatc cacctttaat
     3301 tgtaataata aatacaagca acgtttatac gtcaaaagca tttataaact tttaccctaa
     3361 agtagcttgc ttgtgtgttt agtttataat tagtctctta ttaatttgat gtaggtaagc
     3421 ccgccacaaa tatatatttt taacaagata ccgtggaaaa acttcgtgct atcacaaaac
     3481 agtatacaaa aaataagctt gtcgacgaat tcagatatca cgaattctgg atccgatacg
     3541 taacgcgtct gcagcataag cgtaggtcaa ttgcgacttg gaggttaatg ttcacaccag
     3601 tcgtgaaaac cacaaagtcc tcctcatccg aaggagagct gacttccgac tgcacctcac
     3661 ccaacgcgcg cgtcgacacg aatcccataa cgaagttctt gtatttccgg gcataatcga
     3721 ccgaggaagt agtgtactgg ccggtagcca aggagccctt agaggtcatc tctgccaaga
     3781 tcaacagacc gcgttcgggg ccgtaggcga agtccggtgc agacgccgtc tgagcgagag
     3841 cctcgacgat accctcacca gggagaatgc tgcagttgat gatgtgggcc cattccgaga
     3901 tacggagggt accgccgtgg tattgcttct ggaccgtgtt gccgatgtca atgaacttgc
     3961 ggtcctcgaa gatgagaaag ttgtgcttct gcgcgagagc cttaagtccc tcaatagtct
     4021 cgttgctgaa atcagagagg atatcgatgt gggttttgat cacggcaatg tagggaccga
     4081 gacggtcagc aagatctagt agctccttag tggtggtaac gtcagccgag acagtcacat
     4141 tggttttctt ggcctcggca atctcgaaca gcctcttcgc cagagcattg ggatgcttgc
     4201 tggcacgggc agtgtaggtg agctgggact tgctgctcat gtgtcctggt gggtgggttg
     4261 caatgacggc agacaaggca aggacgtttg gagatgaggg agtttggagg ttgaggtgag
     4321 tttgaagccg cttcggcccg tggtcgcctg cccttagtca gcgccgggga ttgattggtt
     4381 gcggagcttc tccggctgcc cgcagtgacc tggtccgcag acctactcga ttgggtgtcc
     4441 ggcttagggc ttagggtcta taatctgctc cagtggtcat ctgcggtctg ccgtagataa
     4501 ggaacacatg ccccggccga gcaatggccg cgttcagccc gggatcagcc ggtgctaaac
     4561 tgcgagagcg gacgctagtg tcggttcatt gcgaacgaac aaatgccggc gccatatgtt
     4621 cccccgggct aaaatgaatt atcatacatc acttaatacc ttacatcaac ccgtccctat
     4681 agtgactcag agttatgctg atcatcctcc cgtgcctaca acaagcctgc accaccagcc
     4741 ctatcggtga cccgacattt gaaccaatat ggagagtaag gagatcagag ggtccaagca
     4801 gggccgaatc tggcgacagt agatataaat atgaaaattc cccaacgctt ttactcatgg
     4861 agctgcatgg ctttctcgcg gcaagcagct ggggtgaagc catttccaac cttccctgca
     4921 atgatgcgcc accgatcgtt ctcgtactcg tgcattgccc gctgcagcct ttgtacctat
     4981 tcccttcatt agcatgtgtg agacaggcgt agaaatacat caggagcaaa tcgaaccatt
     5041 tcgtcactcc aagctacatc cttagccttc agcttggtgc agtatcggac ttgcaaggta
     5101 ccgctcgttc ggcccgggaa gaagtctgca atttgcttcc acgtcaagtt cttgttttcc
     5161 ttcaactcca ccagtagagc gtcctcttcg ggagtaaact tcagttttgg tcctcgaggc
     5221 ctgggggctg gatcaggcat tccaggctgg cccaccacac tcggcgggcc cgagggcaaa
     5281 ggaggagatt ccacattcac tccatgacgc cctggggcgc gcagcgctgc atggggcggg
     5341 agtcgatggt gatggtgatg ttgcggcagg gccacagagt ccgtgggccc aaagccatgg
     5401 tttgacatgg gatgctgctg agatgatgcg ccatggacac cgaagagcat ttcgggagag
     5461 tgcgagggtt cgtgaggggc gtcagctgag aggactgata gctgcggccg caggccatgg
     5521 tcggggttcc catcctcttc ggttcatgaa ccttgtgccc gtctccccgg agcgagtgga
     5581 gatgtggagt gggcgcttac acagtacacg aggacttcta gaaagaagga ttacctctaa
     5641 acaagtgtac ctgtgcattc tgggtaaacg actcatagga gagttgtaaa aaagtttcgg
     5701 ccggcgtatt gggtgttacg gagcattcac taggcaacca tgcatggtta ctattgtata
     5761 cccatcttag taggaatgat tttcgaggtt tatacctacg atgaatgtgt gtcctgtagg
     5821 cttgagagtt caaggaagaa acagtgcaat tatctttgcg aacccagggg ctggtgacgg
     5881 aattttcata gtcaagctat cagagtaaag aagaggagca tgtcaaagta caattagaga
     5941 caaatatata gtcgcgtgga gccaagagcg gattcctcag tctcgtagga ctcttgacga
     6001 ccgttgatct gcttgatctc gtctcccgaa aatgaaaata gactctgcta agctattctt
     6061 ctgcttcgcc ggagcctgaa gggcgtacta gggttgcgag gtccaatgca ttaatgcatt
     6121 gcagatgagc tgtatctgga agaggtaaac ccgaaacgcg ttttattctt gttgacatgg
     6181 agctattaaa tcactagaag gcactctttg ctgcttggac aaatgaacgt atcttatcga
     6241 gatcctgaac accatttgtc tcaactccgg agctgacatc gacaccaacg atcttatatc
     6301 cagattcgtc aagctgtttg atgatttcag taacgttaag tggatcaagc ctagttgccg
     6361 ttggctttgg ccttgagtct cctttcgcgg gcatgtcgcc agccaggacc ccatcgatct
     6421 tcgacgtaca gcaacggagt catcacaagg gtgatgccgg caagcaagag gaaagtataa
     6481 tccggcttca aggcgtccag gataggttgc acgatagcca caccgccagc tccaagcagg
     6541 caccgcatca gattgttcac tgctgtcgca ctggcgctag cacctgggta aagatcgatg
     6601 accagggcgc tgttgatggt gaagagtcct gttgagcaga acgcaatgaa gtactgcaga
     6661 atgataggca ccgccagatg tgtccgcaaa gacacgccgt acaaagcaac tgtcacgatg
     6721 aagatcgcaa tcacccacca ggtattgcgc atccgggcga cctcaatggg gaagtcgggg
     6781 tgtgatttca gattgacacg tgtgcctgcc ggataaccgt gtttctcgca atattcgcgt
     6841 tcggtaagac ggtggttgta atccataagg tagccgacca gataagagcc agacatacat
     6901 ccaaagccat tgcctaggaa agtgagtcca atgtccaggg atgacaggcc gtacacttcg
     6961 ctgaagaggt cggtggtact ggatgtcacc atgctccaca ctgtgtacac gatacttcca
     7021 aagaacaggg tgatgaaaac gtccttttcg acgaggaatg tcaaaggagc aaaaacagac
     7081 ttccagctgg ttttggtctt tttcgcttcc ggttgcgctc cctcgacaac ccccgtctgg
     7141 ccggtgatcg tgtagatgaa gggcttatga atgccattga gcttcacagt tccatttcca
     7201 gcaattggcc tcaatgtctc cggaagaagc accagaatgg acaggagact cacgcctcca
     7261 gcaatcgtga ggaaccaaaa gatagatcga tatccgagat actgggtgaa aatgccgccg
     7321 aaaaccggcc cgattccctg tccaagcatg cgaactccac cgaagatacc caccaagcta
     7381 cctctttctt ccgagtttgt gatatcacca atcactccag ctccgattga gatggtggct
     7441 gcgctaccag cagcctgcaa ggctcggaag gccatgagct caccatagtt cttggattcg
     7501 gccagagcaa tattggctac gaggtaaaca atgaatgttc caataaagac aggccgtcta
     7561 cctgtggcgt ctgacatgga accccagaag ctgggagcga ggccttggac gatcatgtac
     7621 accgtgatgg tgagtgttgc gagcgacatg ctgatgttga gggatttcga gacatcatcc
     7681 agggcaggga agtaaatgtt cgacgaaagc ggagaaaata tggcagcgag ggagacgatg
     7741 caaaccattt gcagcttgcg agatcgtgtg aagatatgat gcggtggctt ctcggatgac
     7801 tgagcctcga gggtcgaagg ctccggagag agggatccct tatcggagtc actgtctcta
     7861 ggggtgacct gtggtacggg gaccttttca aggtctgatc ttgatgagga cgtggttgag
     7921 gacatgtttg aagatggatg agaagtcggt gatgagatag acgttctagc ggtgatggtg
     7981 ggttgattaa ggggtgtggt gtgggtggag gatgtgaggg tggcggtgtg tgagtgtcaa
     8041 aatcgatcgc gtgaggtgtg tcgtaaggag ggatgttcaa ggcccgctga atttatatcg
     8101 acgccacgga agcttctcga gccctgggat ggcggagtca gaggggcggt atgcgctagt
     8161 ccgctttggg ttagggctgc tgacatcatg gatcggccaa tctttccccg tacgttgccc
     8221 gattgttggc tgaggttctg ggctgcgctt tgcttggggg tttgtgtaag gtgagtcaga
     8281 gtgagtcggt tgtctgggat tcttattgat tatgctgtga tgacagtggt atttgtgctt
     8341 agtgctgatt aggaatatgc catttgggtg gtgttgtttg gctgtaatta ttgtgctggt
     8401 tcgtccatag ggtgtatatt actatatctg gaagtaagtt gagtcttgta tacggtagta
     8461 taggtccgag gtgaagagag ataaaatcaa tacactgctt ttaactggtg tcatattttc
     8521 acatcaatat gccctatttt attggcgttc gctggctttt atagtgtgta aagacatata
     8581 catgctcagc gttatttgtc caacccaata tactgatgaa ctatgattcc gtggtccagc
     8641 acacaccatg tcctcatcct gacgctgttt ctactacgtc agtatacgta aatataatgt
     8701 ctggcagaag gattctcttg acttccagac ttaactttcg aaaaacaatc aacgaaaatc
     8761 aagggaaagc agaggacgcc atcaaagatg accacccaca agctctctcc gacatgcgag
     8821 tcacatgcgt cgctgatgct gaaggagact atccacagac cctttgatct aatgtcagat
     8881 atactcaggc ctatctggct ccgggtgatc aaagacgaac gctacaatct cagacgtcgt
     8941 ctctatgaca gggcaagatt atatgagaag cataggccaa gcaagtgcaa catccaaaaa
     9001 gactggatca agcgctacaa ggagaacgag gagctacagt tcgccctcct gcagcggatt
     9061 aactgtctgg aaagggtcaa atacagacgt ggtattgagc cagaaaatcc cttccagcat
     9121 gtcccggggc ccgcggatcc gaaacgtcaa gtctcccttg cagaagagca ttactaccgg
     9181 tgccagaagg catggaaagc gttgaccctt gacaaaccat ctggtcctat catcgacgag
     9241 tttgacttcc ttcagcatca cagaaacgaa gagggccaaa ctttggcatg ggaggaggac
     9301 aagctcatgt gcgcctcgct tggtggctgt tgcggtcgca gatgtagatg ctgtgagagt
     9361 cctctgcggc actatctctt gcccgagggc ggccagaagg aaatgattgg atttcatggc
     9421 cactgtacct ccgatcgcga aaaaaccccg ccctgtcagg ggcggggttt tttgcgctcc
     9481 agtttccttg ccattgactg tgccactccc gaggtccatt ggagctcgta ttccctgctg
     9541 cacatggaga aatagaccac gtcagtcaga ttgccattgt cattgccgac cctagcagca
     9601 cgcatagtaa aatgcgttga atagtagtat acagtagaga ttattacata tagctggtat
     9661 agccaaacat cgccaatcac ctcaatcaca ccacggattc agtccaaact tagaagtagg
     9721 aaatcaactc cctcgcaact ccaaaagacg tcaagcccca gagagctccc tgtgcaagca
     9781 agtagaagct gccgtacaaa cgaccgcgac cccgcggctt gccggagtac acatatccgt
     9841 aaaggaacag tgagcgaccg aggacccaga tgcttccgag gccagtcgcc aactgggggt
     9901 acttcagtcc agctaccagg aggaagagca tagtttggct ggagttctca aggaagttgg
     9961 catgagcgcg ctgagcgcag ttgaactgct cggccttggg ctgcacggtt cggaatgtat
    10021 gttagctcga ggaatcctgt ttgtgtgagg ttggcttacg ttggtcttgc actgctctac
    10081 ggtcgcatag cagtgagggt aggggcaatc agcttccttg cggagacgag acacgacggc
    10141 gccatggacg aagctcagga cggggatggc acccagagcg acggcaatga cagagctgca
    10201 ggatagttag catcagactt catggggaga atggcattgt ctgcaaggaa ttgtagaatt
    10261 ggcacatacc cgtagttttc agggacggtc agggtaagca tgatgggtga tatttacttg
    10321 tggtgttctc agcttgaatc tgctatgact aggcggtatt aggaagtcta gaagatgccg
    10381 aaagtagttc gatttatagt cgggcgattg atggggcgca agagcggccg gatgcggatc
    10441 cagagcatcc ttggcggatc ccttcctcca agtcgcaatt gacctacagc gggccgcata
    10501 tcattcagga cgagcctcag actccagcgt aactggactg caatcaactc actggctcac
    10561 cttccggtcc acgatcagct agaatcaagc tgactagata aactggccgt cgttttacac
    10621 gggtgggcct ttcttcggta gaaaatcaaa ggatcttctt gagatccttt ttttctgcgc
    10681 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    10741 caagagctac caactctttt tccgaggtaa ctggcttcag cagagcgcag ataccaaata
    10801 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    10861 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    10921 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    10981 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11041 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11101 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11161 atctttatag tcctgtcggg tttcgccacc tctgacttga gcatcgattt ttgtgatgct
    11221 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc agaaaggccc acccgaaggt
    11281 gagccaggtg attacattta ggtcctcatt agaaaaactc atcgagcatc aagtgaaact
    11341 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
    11401 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
    11461 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
    11521 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaag agtttatgca
    11581 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcac
    11641 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgccgt
    11701 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
    11761 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttccctg
    11821 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
    11881 gaagaggcat aaattccgtc agccagttta gcctgaccat ctcatctgta acatcattgg
    11941 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
    12001 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
    12061 cagcatccat gttggaattt aatcgcggct tcaagcaaga cgtttcccgt tgaatatggc
    12121 tcattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
    12181 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaagt caaaagcctc
    12241 cggtcggagg cttttgactt tctgctatgg aggtcaggta tgatttaaat ggtcagtatt
    12301 gagcctcagg aaacagctat gacatcaagc tgactagata atctagctga tcgtggaccg
    12361 atcatacgta taatgccgta agatcacgcg aagctattct gcattaacgt cagagcacag
    12421 gctggttaac atgaccagac tgatcgccat aggctcatgc tccgcagctg taggcaattt
    12481 gggtatggtt tccacgtgca cttacatgtc atggagaagc gcaagaggtg gagtaattgg
    12541 aatctaccag cttgatctat aaacagaaat tttggttgcg atggatcagt acagccatgt
    12601 tgcaccccgt gtatcctcat tcaagcaagg ttgcgcagtt ggcaaagccc tgctcctgtc
    12661 acgacgtgct ttcgctggta ctcggagttg ttatacgcta ctcgactgcc gaatcgatct
    12721 ttagatcaca gacacagttg actttattct cggagtatgt ctaatagaca ctcgacattg
    12781 tggatagatg ttctctctct ctcactagct actgatcgac aaagaccatc catgtgaatt
    12841 atagtatcgc tggcaacaat ccatcagttg ctcatttgcg gtctaccgac ctgcgacaga
    12901 caaccaaagt agacaaagac tccgccaacc gggaaccaca ccatcgcaca tcattgtttg
    12961 aagcccggtc acgaaagcca tgcatctatt cctttgccct cggacgagtg ctggggcgtc
    13021 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc
    13081 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac
    13141 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa
    13201 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc gggtgcctcc
    13261 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt
    13321 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta
    13381 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga
    13441 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac
    13501 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata
    13561 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc
    13621 tcgtctggct gagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa
    13681 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga
    13741 tgcaataggt caggctctcg ctgaattccc caatgtcaag cacttccgga atcgggagcg
    13801 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat
    13861 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc
    13921 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcagg aacttctcga
    13981 cagacgtcgc ggtgagttca ggctttttca tctagaggtt aagaggttct tccggcttcg
    14041 cgataaggta ctggaggaga aacaaaagtc aagccggt
//
